CANNABIS DNA PROJECT

[Rolando Mota]

Thought this was cool, the "phylos galaxy" will be made public on their website April 20!


http://phylosbioscience.com/the-phylos-galaxy/

 

[Londinium]

So we have a date...thats good news,thanks

 

[Manivelle]

hi sam
i would know your experience with herbarium museum etc.. please ?
you said they are hard to locate.
if you're interested i can give you a few places in france where you'll be able to find some .
let me know.

 

[FvG]

Hi Sam,

This looks like a really cool project! I am a biologist and interested in the cannabis genome sequence (see also my forum post here). Do you know what the status is of the PacBio sequencing and assembly?

I would love to get in touch

 

[Sam_Skunkman]

Send me a list of Herbariums with the accessions names and numbers if you know them, yes we are interested and have contacted many herbariums mostly online with databases we can review.
-SamS

hi sam
i would know your experience with herbarium museum etc.. please ?
you said they are hard to locate.
if you're interested i can give you a few places in france where you'll be able to find some .
let me know.

 

[Manivelle]

it is 1:55 the 20th of april here in france . can't wait

 

[Manivelle]

i'm really disapointed

where are the landraces ? found thai and laos....
did i missed something?

a lot of US strain some with multiple entry and none marked as "original" or "creator" or legit .

navigation in the galaxy is not optimal. i was not able to read when there was too much strains in an area.
not able to translate on XY axis

i hope you will make a special galaxy for landraces only because all those renamed strain are annoying as hell .....

crawling back under my rock now ....

 

[Mustafunk]

Yeah that Phylos Galaxy thing looks like a gimmick for newbies, it totally looks just like genealogic trees at Seed Finder... and we knew the family trees of modern hybrids already.

So is this the Evolution Project we've sent samples for? I was expecting some serious research paper releases on Cannabis evolution to be honest. At least a cladogram or something instead of that fancy galaxy thing that seems to be pretty useless for the researcher and going through the results of the DNA tests.

I really hope they will publish some papers anytime soon or at least something similar to what Kevin McKernan was doing at Medicinal Genomics.

Vibes.

 

[PrimalHaze]

I think the phylos galaxy is pretty cool. Although I thought there would be more landraces represented, it looks like it is more california slanted.

There seems to be big spikes for trainwreck, OG , Diesels and the cannatonics with a big glob of hybrids in the middle. Afgoo is everywhere on there. Trainwreck is wayyy out there in space. I need to get me some. HAppy 420!!

-pH

big spike on GDP too

 

[CosmicGiggle]

I think the phylos galaxy is pretty cool. Although I thought there would be more landraces represented, it looks like it is more california slanted.

It seems to be a tease for more to come in the future but looking at that 3D revolving map it does seem as though California is the center of the Galaxy.

 

[Manivelle]

APRIL FOOLS

 

[99%]

What do the experts see in this 3D map of Cannabis DNA? I'm really interested to read their analysis.
Is Skunk the ancestor to most modern hybrids?
Where did cannabis originate from?
Just how polluted are the world's landraces?
Are Colombian, Indian and Thai the building blocks to Haze?

I bet there are lots of other questions too.

 

[99%]

also which modern seed lines are marketed as having parents for example A and B but actually are C and D?

 

[99%]

What about seeds from 100, 200 and 1000 years ago that were tested...what can we see from their DNA?

 

[99%]

Where did Moroccan strains originate from?

Are there any landraces or strains that are totally unique and very different from the DNA of all the other strains or landraces?

Does this 3D map show that almost all modern strain's DNA are contained in a narrow bottleneck?

 

[99%]

I can't believe the lack of comments on the the 3D DNA map of cannabis strains since it was published...I'm still waiting on those smarter than me to explain all the findings.

One thing is for sure that they need to (if they haven't already) show a DNA map of only landrace strains, I thought the main thing this project was going to show was the origin of cannabis, where it first started. has this been addressed yet?

 

[Thule]

I can't believe the lack of comments on the the 3D DNA map of cannabis strains since it was published...

No useful data, so no comments I guess. If there are only a hand full of landraces on the map one can't make many assumptons, and to be frank, mapping the relationships between established hybrids is not very interesting as they're basically all ghani hybrids.

Are they going to add more landraces on the map or is this it?

 

[Sam_Skunkman]

Phylos will add the first 112 samples I gave them soon, they have DNA sequenced them and many are landraces, I have several hundred more samples from contributors that need the DNA extracted so the DNA can be posted to the USA. We also need a real version of the Phylos Galaxy, (the version released is just a Beta version) so in a real version contributors can search by Phylos sample numbers, so far #2000-2111 from me. I am sorry things take so long but it is the problem of extracting the DNA as that is all that can be sent legally to the USA for analysis and sequencing. Almost everything on the Galaxy so far is Poly-Multi-Hybrid so it is not helping with understanding evolution or building a Cannabis family Tree that will show relationships. We need thousands of landrace samples, from every Cannabis traditional area, be they Ganja, Hashish, Hemp, or just wild. Most of the present Galaxy samples were from American N West growers that grow Poly-Multi-Hybrids, but they do not help with evolution or a family tree, maybe a bit with relationship studies to see where they come from?
I will test my really old seeds when we are sure they can do it and not waste them, I just have a few of them, 500-2500 years old, maybe 10 different batches. For example I have 8 Cannabis seeds from the Barrows 2-5 they were excavated by Sergei Ivanovich Rudenko in 1947-1949 Pazyryk burial tombs 2500 years old. In a corner of one grave chamber of the Pazyryk cemetery was a fur bag containing cannabis seed, a censer filled with stones, and the hexapod frame of an inhalation tent - these are believed to have been utilized at the end of the funerary ritual for purification. (My 8 seeds are from that bag, given to me by the Hermitage Museum)
I did heat treat some of my different fresh hemp seeds to make them unviable and legal to post to the USA but heating them also, when we tested them, we found the heat damaged the DNA by fragmenting it even more. So we do not want to do that, we need to extract the DNA in Amsterdam and send just the DNA to Phylos, we have started doing that.
-SamS

 

[Genghis Kush]

super interesting!!

thanks for all your work Sam Skunkman!

 

[99%]

Awesome Sam.