Hi Sam how you been?
How is the DNA project going? Anything new to share results wise?
Cheers!
How is the DNA project going? Anything new to share results wise?
Cheers!
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CANNABIS DNA PROJECT
- Thread starter Sam_Skunkman
- Start date
It is not as easy cut as that.
You first need a data base to reference your findings to.
So you first run the stuff you know for sure the origins. and if you have enough true representations of such, you have your reference point.
Lets say you even have a verified Greek variety or landrace, and your sample is very closely related, or not... or lets say you don't have in the reference data base anything from Greece, but you have from very close regions, and according to what is known historically about cannabis migration, it makes sense that those seeds contain dna from those populations etc...
I dunno how is Phylos doing it, but it should be something or other like this. established reference points etc...
Etruscan,
I do not steal.
Did you send me something I stole? What? I never got anything from you did I? I looked and you never sent me anything and if you do I would use it for the Phylos DNA work, that is why I say I can use dead seeds because I do not grow the materials, a dry leaf will also do, I can not grow or steal anything from a dead seed or dry leaf can I? Many folks have sent in viable seeds, again I do not need to grow them. A few folks have sent me seeds and they said I could grow them if I want to. I have a database divided into dead seeds, dry leaves, live seeds and these are divided into ones to test and ones to test and maybe grow, all decided by the grower that donated the seeds.
I do not need seeds except for the DNA project, I have well over a ton of my own seeds made by me that I have never grown, I just did not have time or space to do them all, more then a thousand hybrids I made in the last 3 decades, as well as what I collected in my travels. More like two tons.
If you have a Greek landrace I want it, pre 1920 the Greeks were the biggest hashish producers in Europe, I wonder if the landrace is from rope or dope? DNA analysis would be able to tell this, and when enough samples have been collected and analyzed what it evolved from and what related to on a Cannabis family tree.
-SamS
I do not steal.
Did you send me something I stole? What? I never got anything from you did I? I looked and you never sent me anything and if you do I would use it for the Phylos DNA work, that is why I say I can use dead seeds because I do not grow the materials, a dry leaf will also do, I can not grow or steal anything from a dead seed or dry leaf can I? Many folks have sent in viable seeds, again I do not need to grow them. A few folks have sent me seeds and they said I could grow them if I want to. I have a database divided into dead seeds, dry leaves, live seeds and these are divided into ones to test and ones to test and maybe grow, all decided by the grower that donated the seeds.
I do not need seeds except for the DNA project, I have well over a ton of my own seeds made by me that I have never grown, I just did not have time or space to do them all, more then a thousand hybrids I made in the last 3 decades, as well as what I collected in my travels. More like two tons.
If you have a Greek landrace I want it, pre 1920 the Greeks were the biggest hashish producers in Europe, I wonder if the landrace is from rope or dope? DNA analysis would be able to tell this, and when enough samples have been collected and analyzed what it evolved from and what related to on a Cannabis family tree.
-SamS
Well maybe some good news about the PHYLOS DNA Project.
They will begin to release the info:
"We’re going to make all of our sequencing data public in about a week, through the Open Cannabis Project website, which is being rebuilt now. It will be our current PacBio genome assembly and a growing set of raw short-read data from targeted amplicon sequencing and GBS.
The OCP doesn’t have the funding or infrastructure to host the data itself, and in any case we’d prefer that all the data be searchable, etc. So the OCP website will just contain links to the data on NCBI. There will be a list of contributors, each of which will link to a contributor page, where there will be a list of links to each accession."
I am not sure how this will work and when you can search the PHYLOS Galaxy by a PHYLOS sample number, as I have only given out a little more then 100 Phylos sample ID #'s, the rest I still have the samples and am waiting for Phylos to send a lab tech to Amsterdam to extract the DNA so it can be transported legally to the USA. That should happen very soon. Then they will be given a Phylos sample ID # and I will let each contributor know what theirs is. There is also the issue of privacy, both for the donator and the DNA info generated, the info is yours, but we would like to share the info through the OCP, if you do not want this for any reason you need to inform me. I will try and ask everyone by PM. Up until now they will all be listed as given by me, but I am happy to change that if you desire it to be your name or whatever. For me the focus has been to get the info up in a searchable form with donators being able to see where their variety fits into the big picture in terms of relationships and evolution.
This has take a lot longer then we had hoped, but the light is at the end of the tunnel.
-SamS
They will begin to release the info:
"We’re going to make all of our sequencing data public in about a week, through the Open Cannabis Project website, which is being rebuilt now. It will be our current PacBio genome assembly and a growing set of raw short-read data from targeted amplicon sequencing and GBS.
The OCP doesn’t have the funding or infrastructure to host the data itself, and in any case we’d prefer that all the data be searchable, etc. So the OCP website will just contain links to the data on NCBI. There will be a list of contributors, each of which will link to a contributor page, where there will be a list of links to each accession."
I am not sure how this will work and when you can search the PHYLOS Galaxy by a PHYLOS sample number, as I have only given out a little more then 100 Phylos sample ID #'s, the rest I still have the samples and am waiting for Phylos to send a lab tech to Amsterdam to extract the DNA so it can be transported legally to the USA. That should happen very soon. Then they will be given a Phylos sample ID # and I will let each contributor know what theirs is. There is also the issue of privacy, both for the donator and the DNA info generated, the info is yours, but we would like to share the info through the OCP, if you do not want this for any reason you need to inform me. I will try and ask everyone by PM. Up until now they will all be listed as given by me, but I am happy to change that if you desire it to be your name or whatever. For me the focus has been to get the info up in a searchable form with donators being able to see where their variety fits into the big picture in terms of relationships and evolution.
This has take a lot longer then we had hoped, but the light is at the end of the tunnel.
-SamS
lots of new entry. i'm in heaven . thanks sam . 
do you know if they will make a map for landrace only ? and make the link between them more visible ? because we can't decrypt the infos for now . it's not clear ..
the best would be to be able to change the lvl of the linkage. ; making the link visible for different lvl of proximity. 1 alot of connexions ,2,3,4, to 5 for not a lot of connexions. i don't know howthey have programmed the galaxy but if it could be possible .. ( sorry for my bad english i don't know how to say what i would t say).
do you know if they will make a map for landrace only ? and make the link between them more visible ? because we can't decrypt the infos for now . it's not clear ..
the best would be to be able to change the lvl of the linkage. ; making the link visible for different lvl of proximity. 1 alot of connexions ,2,3,4, to 5 for not a lot of connexions. i don't know howthey have programmed the galaxy but if it could be possible .. ( sorry for my bad english i don't know how to say what i would t say).
Thank you to all those involved in this project! It is wonderful to see the work come to fruition.
I see Sam, you are still being plagued by the people who think you are Mr. Burns, and have dark motives lol
Best vibes to the OCP!
I see Sam, you are still being plagued by the people who think you are Mr. Burns, and have dark motives lol
Best vibes to the OCP!
frostqueen
Active member
The data is up. It turns out there are like 10 versions of every modern popular strain, thus rendering most names pretty much useless. Which 'Obama Kush' is the real deal? Or which 'Blue Dream'? Who decides which of the ten different submitted versions is the legit one?
Unless any breeder still has the parent stock of any given hybrid, all bets are off. The re-name game over the years has been much worse than anyone ever imagined.
That aside, it will be great to start seeing more data on older landrace varieties, and how they relate to modern ones.
Unless any breeder still has the parent stock of any given hybrid, all bets are off. The re-name game over the years has been much worse than anyone ever imagined.
That aside, it will be great to start seeing more data on older landrace varieties, and how they relate to modern ones.
PHYLOS CANNABIS DNA PROJECT
I am starting to get the data but still not as easy to use as I would like, I have another project that is taking all my time for the next few weeks then I will contact everyone that has data up on the Galaxy that I gave to Phylos, about 100, right now they are all in my name but they do have the Phylos ID number I gave to the samples and to each of the contributors. I will explain how to find each sample to any contributor.
I will number the rest, a few hundred more, when Phylos has a plan to come to Amsterdam and extract the DNA so it can be legally sent to the USA. Then we will have a much better picture of Landrace populations as the Galaxy is really at this point mostly Multi-Poly-Hybrids, except for a few like RCC and I gave them, less then 200 I think. This will change and the Galaxy will become searchable by the Phylos ID number, as well as having many more landrace accessions sequenced and added to the Galaxy.
I want to thank all that have contributed DNA to the Phylos Project, and hope your efforts help us to understand the Cannabis family tree, relationships and evolution. If anyone has additional landrace varieties we are still interested, and can use seeds, even dead seeds, as well as dry leafs for the project.
What we want the most is seeds collected in landrace areas, the longer ago the better. The landrace populations are now being polluted world wide with western bred Multi-Poly-Hybrids, it has happened in Mexico, Jamaica, Morocco, and many other countries. This is a race against time to find unpolluted landraces before it is to late and they are all hybridized. Sad but true. We are just custodians, caretakers of the genetic heritage, to pass on to our children, family, friends, and community. We should do all we can to assure that traditional areas of Cannabis landrace cultivation do not allow imported males to be grown and flowered, really the same with imported females if they are allowed to set any seed from local landrace males.
I was lucky to collect seeds in the 70's and 80's but I did not have a chance to go everywhere, or even collect all the different populations in a traditional Cannabis area like India, where I have been 15 times and lived there several years in total. I spent a lot of time collecting seeds, but you must be there at harvest time or buy product to get the seeds, I have done both when needed, and will until I am gone.
Just PM me if you have any landrace seeds to contribute, even if old and dead. We just need the DNA. I have never given a single viable seed to Phylos just dead ones, dry leafs, or DNA from live seeds, so no fears they are using the seeds to develop anything from them, they can't.
Both Phylos and anyone can try and use the DNA info to develop improved varieties, but that is fine with me as long as the info is being shared, and it is.
-SamS
I am starting to get the data but still not as easy to use as I would like, I have another project that is taking all my time for the next few weeks then I will contact everyone that has data up on the Galaxy that I gave to Phylos, about 100, right now they are all in my name but they do have the Phylos ID number I gave to the samples and to each of the contributors. I will explain how to find each sample to any contributor.
I will number the rest, a few hundred more, when Phylos has a plan to come to Amsterdam and extract the DNA so it can be legally sent to the USA. Then we will have a much better picture of Landrace populations as the Galaxy is really at this point mostly Multi-Poly-Hybrids, except for a few like RCC and I gave them, less then 200 I think. This will change and the Galaxy will become searchable by the Phylos ID number, as well as having many more landrace accessions sequenced and added to the Galaxy.
I want to thank all that have contributed DNA to the Phylos Project, and hope your efforts help us to understand the Cannabis family tree, relationships and evolution. If anyone has additional landrace varieties we are still interested, and can use seeds, even dead seeds, as well as dry leafs for the project.
What we want the most is seeds collected in landrace areas, the longer ago the better. The landrace populations are now being polluted world wide with western bred Multi-Poly-Hybrids, it has happened in Mexico, Jamaica, Morocco, and many other countries. This is a race against time to find unpolluted landraces before it is to late and they are all hybridized. Sad but true. We are just custodians, caretakers of the genetic heritage, to pass on to our children, family, friends, and community. We should do all we can to assure that traditional areas of Cannabis landrace cultivation do not allow imported males to be grown and flowered, really the same with imported females if they are allowed to set any seed from local landrace males.
I was lucky to collect seeds in the 70's and 80's but I did not have a chance to go everywhere, or even collect all the different populations in a traditional Cannabis area like India, where I have been 15 times and lived there several years in total. I spent a lot of time collecting seeds, but you must be there at harvest time or buy product to get the seeds, I have done both when needed, and will until I am gone.
Just PM me if you have any landrace seeds to contribute, even if old and dead. We just need the DNA. I have never given a single viable seed to Phylos just dead ones, dry leafs, or DNA from live seeds, so no fears they are using the seeds to develop anything from them, they can't.
Both Phylos and anyone can try and use the DNA info to develop improved varieties, but that is fine with me as long as the info is being shared, and it is.
-SamS
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Genghis Kush
Active member
some very interesting information in the galaxy!
thank you for your work.
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interesting to see how closely related a lot of the landraces are from different parts of the world.
Must have been a relatively recent diaspora for the bulk of the tropical varieties.
thank you for your work.
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interesting to see how closely related a lot of the landraces are from different parts of the world.
Must have been a relatively recent diaspora for the bulk of the tropical varieties.
GMO weed? i hope not. I'm glad europe is kinda protecting us against gmo. From what i heard, US gmo were supposed to bring more yield and resistance to pests and diseases, but it's quite the opposite, europe has now better yields and less pesticides...
I have never worked with GMO and will never do so. I really do not even eat GMO foods like most Americans. I love DNA research that reveals the inner works of Cannabis and will allow me to breed using classical breeding enhanced by the DNA info gained. 100% classical breeding will still be what I know and love. But I will have tools that allow screening of thousands of individuals when they are very very small, allowing larger populations to be screened, and the very best used instead of a sister that is not quite as good for the goal.
We have just scratched the surface with Cannabis, we will find many more interesting possibilities, remember there were no all female Cannabis seeds before the late 80's, the same with Autos, none before the late 1980's. I can think of several new Cannabis techs and understanding Cannabis DNA will be at the core of them.
-SamS
Will you be working with gene editing? It sounds like to me at least, that you will be using markers that you or others have identified as desirable traits will be screened for so thousands of seedlings can be tested by dna and undesirables will be culled then crosses made and start the process over again after all analysis is complete. If this is true I am very excited, the gene editing is really a good promise too, being able to knock out genes to get the attributes you want in a more controlled and quicker succession is really progress. It is better than GMO in my opinion as you still do the classical breeding but you have tools to help do selections with solid evidence and not just eyes, and lets face it everyone's eyes change every day depending on what your mood is or sleep you have had, and I have yet to meet anyone who has a pcr or gc in their eyes.
What's this about GW Pharma, Hortapharm BV and GMO's?
Australia legislates for 100% GMO Biotech Cannabis not Marijuana
Yeah, that article clearly states synthetic strains from Hortipharm,
That's you and Rob,is it not? so can you clarify please Sam.
Is there any published info on this dna project? I mean actual usable data?
What's to bet some of those strains sent in (for dna testing)start get used in these
Frankenstein big pharma strains.
That's you and Rob,is it not? so can you clarify please Sam.
Is there any published info on this dna project? I mean actual usable data?
What's to bet some of those strains sent in (for dna testing)start get used in these
Frankenstein big pharma strains.
Exactly on par with your usual level of contribution Corky - in other words complete bullshit & distraction that has zero basis in fact.
When you continue to let your idiocy and anger towards specific people drive your conclusions and posts, rather than do any research or attempt to inform yourself & understand a topic yourself, you end up posting a bunch of delusional nonsense that lacks any factual basis.
That article references "GMO Cannabis", but doesn't cite a single source or incidence of GMO Cannabis. The closest it comes to defining such is this paragraph:
"GMO Cannabis – Cannabis made from genetically modified seed science and used by the likes of GW UK, some call this synthetic Cannabis mostly from seeds by Hortapharm BV in the Netherlands, and the preferred supplier per the Australian Government and is legal in Australia when provided by an authorized supplier in a non-flower form."
Neither Hortipharm nor GW Pharma use any GMO Cannabis, nor biotech methods in producing cannabis varieties with altered chemical profiles. The article incorrectly refers to Epidiolex & Sativex as GMO, & then states that people have died during the clinical trials for Epidiolex, another complete fabrication.
1) Hortapharm is not in negotiations to provide seeds to the Australian government.
2) Hortapharm has specialized (for the past 20 years) in creating cannabis varieties exclusively using classical breeding techniques while eschewing biotechnological means.
3) The article makes the incorrect assumption that non-GMO cannabis is THC-predominant, with varieties producing significant amounts of CBD as being assumed to be GMO. This is patently false, and should have been picked up on by anyone with even a modicum of understanding about cannabis.
It's really sad that the same idiots repeatedly post mis-information trying to slander the better and more educated contributors from our community.
People should actually the read articles they post as reference to their argument, or better yet refrain from posting in threads which they clearly have zero understanding of the topics at hand.
I read that article and.... I believe they are not using "GMO" correctly. I belive everything in that article can and more than likely is accomplished using classical breeding practices. I've noticed the term used incorrectly many times in England and South America.
Sams gonna bet you a naked run through the streets! Oh god.... I just swallowed a little bit of throw up!
Sams gonna bet you a naked run through the streets! Oh god.... I just swallowed a little bit of throw up!
All the data for the OCP will be published here:
http://opencannabisproject.org/data/
Total speculation with zero basis in fact. There are no "strains" sent in to Phylos, just DNA samples. No living material from any samples sent in by IC growers to Sam_Skunkman ever made it to Phylos, IE- not a single living leaf, cell, or seed. Not one. If that is the case, how could "big pharma" use the DNA to produce anything? DO we now live in some Jurassic Park world where DNA can be assembled and turned into franken-plants? Laughable science fiction.
Let's keep this discussion on topic and try to keep the thread limited to facts and science, and leave the speculation and paranoid stoner-musings elsewhere...
