breeding for terpenes

[Sam_Skunkman]

"Nobody said that beta-caryophyllene gets you high. It is a full CB2 agonist that has been shown to displace other cannabinoids. It's also been proven that CB1 and CB2 form functional heteromers and negatively modulate one another. Therefor it's not much of a leap of faith to assume that beta-caryophyllene present in high enough amounts modulates THC in at least those two ways. That's just one example. "

I have not tested if beta-caryophyllene effects the high from Cannabis in a negative way, with a test of 100% pure THC (25mg) spiked with beta-caryophyllene and vaporized I did not notice it. I was really looking for any positive effects on the THC effects, there were none.
I do not like the smell or taste of beta-caryophyllene, it smells like hemp to me. I have smelled a lot of hemp in my life....
-SamS

it seems like a critical thinker might review the available evidence, explore these compounds themselves, analyze their current strains to become familiar with the most prominent compounds present, and not simply draw a line in the sand which seems tantamount to waiting for clinically available cannabis.

There's plenty of double blind studies. They're just not focusing on the psychoactive characteristics. It's not as if GW Pharma hasn't studied their area of expertise extensively. I wonder why there isn't more research into the psychoactive effects, I heard they're passing out licenses like candy. I also wonder how so many pharmaceutical companies can still be in business basing their businesses on such quasi scientific approaches.

The Wachtel study seems to fall short of anything remotely close to conclusive. It's not much of a placebo if you can identify a difference based on taste. Also the lack of less prominent cannabinoids, and unknown levels of terpenoids seem like a significant issue when attempting to identify ways in which they might modulate THC.

It's not difficult to order some essential oil and prove anecdotally to oneself that terpenes matter. Nobody said that beta-caryophyllene gets you high. It is a full CB2 agonist that has been shown to displace other cannabinoids. It's also been proven that CB1 and CB2 form functional heteromers and negatively modulate one another. Therefor it's not much of a leap of faith to assume that beta-caryophyllene present in high enough amounts modulates THC in at least those two ways. That's just one example. Are we better off remaining in the dark age until the gatekeepers provide clinically available cannabis?

Does it make sense to claim that all of the scientific evidence is worthless and that these compounds simply aren't relevant, even though there is absolutely no evidence to support that?

 

[Sam_Skunkman]

Was the THCA 100% pure? How was it purified to 100% THC, was it tested? by whom?? Otherwise it is not really a fair test. You need the 100% pure THC to understand THC's effect alone.
Wachtel's work was just scratching the surface it was poorly designed and executed, the materials used were a joke.
That said what about my work, does that count at all? Or all the people that have confirmed what I said about terpenes modifying THC effects?
I am pretty good friends with both Dr ElSohly and Dr Russo, I told them both about my work as soon as I did it.
You do not want a mixture of essential oils like hemp oil, try just limonene with THC or just Myrcene with THC, they work for sure. A terpene is not a terpene anymore then a Cannabinoid like THC is not the same as a Cannabinoid like CBD. A mix of essential oils extracted from a very potent highly fragrant bud would do it for you with pure THC. I know because I have tried that. As well as trying 25mg pure THC first then adding 12.5mg 100% pure THC to 12.5mg dry sifted resin, that was naturally high in terpenes that I tested at 50% THC on my GC, so the mix was 75 % THC and 25mg of it were way stronger then the 25mg 100% pure THC when vaporized, why is that? All 12 testers said there was a huge difference. all preferred the mix over the same wight of pure THC. Why?
Don't worry about if it won't work for you, that is called projecting, you know the results so why try? I don't think you are crazy, lying, or even fucked up. I think you could be using the wrong materials to test the validity of the idea.
As for a cart before the horse, you mean like all these years Cannabis smokers have presumed that it was THC interacting with all the other Cannabinoids in Cannabis that were creating all the effects found in Cannabis?, two problems with that, Cannabis bred and grown in the west is basically only THC with little or none of the other Cannabinoids, so where did all the different effects from different Cannabis come from? That and if you find a 10% THC only Indica WLD variety like Afghan and a 10% THC only Sativa NLD variety like Thai the effects are very different, the WLD is narcotic, physical, couch-lock, the NLD can be up, clear, speedy, soaring, euphoric, psychedelic, with no ceiling. Why is that? They both have exactly the same Cannabinoids, 10% THC. I did this test also with a dozen others experienced smokers that agreed in their findings.
People also presumed that THCV was psychoactive, more then THC even, it is not even psychoactive, I smoked 100% pure THCV, it is more like CBD. And before people all thought that all the Cannabinoids were made by the plant from CBD. Now we know it is in fact CBG they are all derived from. Life changes, I bet you can too if you try, or want to.

Enough, I can lead to water but I can not make anyone drink....
-SamS

Well I have smoked THCA and found it no different than the NL5xHaze buds it came from, and said so here. It's the foundation of my doubt. I don't think there's anything for me to be wrong about Russo since all I said was he hasn't produced anything like Wachtel, and he hasn't as far as anyone is saying. That's all I've said in all of my posts, but Only Ornamental and especially in vivo seem intent on putting words and thoughts that I didn't say in my mouth. There might be enough material to try another THCA extraction in a few weeks, but there is a scheduled UA after that, so it might be a while. Essential oils - perhaps hemp oil itself - could be arranged. What if it's the same result again? Am I totally fucked up? Lying? Crazy? This is why something definitive on the terpene subject such as addressing Russo's concerns on Wachtel et al. is necessary. This whole pharmacological basis that needn't have even come up here is built up and ready to go, yet it's the cart before the horse.

 

[harry74]

People also presumed that THCV was psychoactive, more then THC even, it is not even psychoactive, I smoked 100% pure THCV, it is more like CBD


Sorry, what you understand for psychoactive ?

Even tobacco ( nicotine) is psychoactive IMO

 

[Sam_Skunkman]

People thought that THCV was an even stronger version of THC, and it was responsible for much of the potency found in really strong Cannabis. By Psychoactive I mean in this case it gets you high, THCV does not. I do agree that native tobacco's are psychoactive at first at least until you become addicted, but it is not for me at all. I can not stand tobacco or its effects at all, makes violently ill, just the smell burning or not, the living flowering plant is real bad for me. Maybe it is the tobacco terpenes? I grew a whole bunch of different wild and cultivated tobacco's some were so strong that if I touched the living flowering plant my hand turned red with a rash.
-SamS

People also presumed that THCV was psychoactive, more then THC even, it is not even psychoactive, I smoked 100% pure THCV, it is more like CBD


Sorry, what you understand for psychoactive ?

Even tobacco ( nicotine) is psychoactive IMO

 

[harry74]

Ok. Tobacco does get you high; may be is a very subtle high.
I´ve been a smoker for 25 years and If I stop smoking 24H and smoke again I can feel the high.

 

[Crocodile OG]

Is it the type or the amount of terpenes that affect the high?

 

[Sam_Skunkman]

Type for sure, I am not sure about amounts.
-SamS

 

[Dropped Cat]

Sam, I have this thunk of yours from a freebie pack, thanks!
First grow from seed in soil. The scent was crushed black pepper.
It was splendid. Cured several weeks, smoke was extraordinary.

I re vegged that plant after harvest.

Fast forward four years. I have yet to flower a cutting
from that plant that produces that crushed black pepper
smell again. Smoke is still extraordinary. It's my go to.

I've self-ed it and crossed it, still no pepper.

How can I get that darn plant to produce that scent again,
where did it go?

 

[Sam_Skunkman]

And all the other grows were they in soil?
If I select a clone by smell normally every time I flower the same clone the smells are just the same. Anything different in the later grows? It is not so easy to predict smells even with crosses or selfings, but I am really surprise the flowering smells just changed, I have not see this. I wish I could help you, glad you still like the Thunk clone. Is the maturity? Is the pepper smell from young or real mature live buds, or from the dry bud? I love a great THUNK!!!
-SamS

Sam, I have this thunk of yours from a freebie pack, thanks!
First grow from seed in soil. The scent was crushed black pepper.
It was splendid. Cured several weeks, smoke was extraordinary.

I re vegged that plant after harvest.

Fast forward four years. I have yet to flower a cutting
from that plant that produces that crushed black pepper
smell again. Smoke is still extraordinary. It's my go to.

I've self-ed it and crossed it, still no pepper.

How can I get that darn plant to produce that scent again,
where did it go?

 

[Crocodile OG]

Type for sure, I am not sure about amounts.
-SamS

I guess the better question is what is the golden ratio of terpenes to THC?

Also excuse my ignorance but does cannabis produce all terpenes? If not can the ones it does not produce be added to affect the high?

 

[Sam_Skunkman]

I guess the better question is what is the golden ratio of terpenes to THC?

Also excuse my ignorance but does cannabis produce all terpenes? If not can the ones it does not produce be added to affect the high?

No Idea, you can see in papers published what the % of a given terpene is, and then figure it out yourself. maybe .15% or .30 of fresh Cannabis plants, depending if they were seeded or not.
The yield of hemp essential oil amounted to approximately 1.3 liter/ton fresh weight, which corresponds to about 10 liters per hectare. This was for fresh seeded plants, unseeded the yield was double this. With dry plants the yield is much higher as the dry plants are 90% lighter.

No, maybe 200 out of the 55,000 known terpenes.
Look up terpenes and Cannabis or just terpenes.
No idea, I have not tried any.
Be careful, with terpenes that are not found in Cannabis, are they safe to smoke? Even some found in Cannabis made me feel ill or cough real bad.
-SamS

 

[Crocodile OG]

Thanks for the response Sam

 

[in vivo]

I was being a bit snide. I shouldn't have put words in your mouth. My apologies.

This whole pharmacological basis that needn't have even come up here is built up and ready to go, yet it's the cart before the horse.

I'm not sure that I completely understand your point here. I have no doubt of the effects of terpenes not solely based on reading, but through the process of bioassay as well as having analysis run on some of my material. I think it's likely to become apparent to anyone who begins to view the cannabinoid profile of their chemotypes. I mean, it's been shown that strains can be identified by the terpene profile. If the effects are simply cannabinoids it seems like you'd be able to identify them by such. Maybe that's not an accurate assumption.

When I read Taming THC I thought that the terpenes modulated the affinity of cannabinoids at CB1. That concept kinda sucked. It meant that it's all a big mystery for the foreseeable future. After stumbling across the study from Bedrocan proving that the affinity of cannabinoids aren't being modulated by the terpenes present at CB1, I got a bit more excited. If their effects are primarily independent of the cannabinoids then it's more of a matter of piecing known information about them together, and checking to see how that jives with our anecdotal experiences. The only way to do that is by getting into the pharmacological basis for these interactions.

I didn't realize your foundation of doubt was based on personal experience. I should have come right out and asked. You're obviously educated and I thought you just weren't giving this the time of day.

I noticed that you said you tried this with a nl strain. If you look on the 360analytics site and view the profiles for nl you'll see that they're all high in linalool.

I've smoked two previous bowls today, and now I just put some "Ho Wood" essential oil (98% linalool) on top of the same flowers I've been smoking today. My first impression was an expectorant sensation. Similar to the difference between flowers and concentrate, but more pronounced. As I'm writing this that has all but faded and seems to be taking on more of a sedative like quality. I also had to get up to get some more coffee to get the taste out of my mouth. It's not one of the worst, kinda a floral.

I agree that essential oils are likely no substitute for isolated terpenes. I'm really interested in steam distillation, and recreating aroma, but I've got my hands full with projects currently as is. Limonene can be found easily in high concentrations, linalool is relatively high is some oils, there looks like there's some steam distilled oils from cannabis online. Other than that I think you've either got to jump through a few minor hoops or settle.

If a study looking at less than 20 terpenes in profiles from 17 "mostly sativa" or "mostly indica" chemotypes can begin to identify patterns, I can only imagine what we'd being able to learn from a couple hundred more complete profiles, sharing anecdotal reviews on each.

 

[in vivo]

People thought that THCV was an even stronger version of THC,

THCV has been a bit of a thorn in my side. I keep trying to get the lab I work with to quantify it so I could investigate it. They were all over the place with the pharmacology of it for a while. Now the consensus seems to be that it's a CB1 antagonist. I'm more interested in trying to work in CBDV at this point for the anti-epileptic characteristics. I'm glad that you can confirm the findings on THCV.

You need the 100% pure THC

I'd love to run a solid phase extraction to procure some pure THC, I think understanding it would be of immense value. It seems like if we're talking about the effects of terpenes in cannabis that have other cannabinoids present, that it's not necessarily required. I think there are much better ways than to simply put some on top of flowers, but you could likely clean up any oil with EtOH a number of times until the terpene content was practically nill.

I just took the same flowers again and put some balsam copaiba (primarily beta-caryophyllene and caryophyllene). No expectorant qualities at all. Not noticeable for the fist few minutes. The high seems to shift from behind the eyes to the back of the head. It's difficult for me to describe, but it doesn't feel normal. It could just be my imagination, but I don't think it is.

Joe,

I just noticed that you've got a nl x haze and not a nl. My bad. I have a amnesia that's similar, but it's not high in linalool, it's sky high in limonene. You might be able to look it up to see what others are seeing.

 

[Sam_Skunkman]

THCV has been a bit of a thorn in my side. I keep trying to get the lab I work with to quantify it so I could investigate it. They were all over the place with the pharmacology of it for a while. Now the consensus seems to be that it's a CB1 antagonist. I'm more interested in trying to work in CBDV at this point for the anti-epileptic characteristics. I'm glad that you can confirm the findings on THCV.



I'd love to run a solid phase extraction to procure some pure THC, I think understanding it would be of immense value. It seems like if we're talking about the effects of terpenes in cannabis that have other cannabinoids present, that it's not necessarily required.


GOOD LUCK FINDING CANNABIS WITH OTHER CANNABINOIDS BESIDES THC, IT REALLY IS NOT SO EASY IF YOU ARE LOOKING AT WESTERN BRED CANNABIS THEN IT IS REALLY HARD.
-SAMS


I think there are much better ways than to simply put some on top of flowers, but you could likely clean up any oil with EtOH a number of times until the terpene content was practically nill.

I just took the same flowers again and put some balsam copaiba (primarily beta-caryophyllene and caryophyllene). No expectorant qualities at all. Not noticeable for the fist few minutes. The high seems to shift from behind the eyes to the back of the head. It's difficult for me to describe, but it doesn't feel normal. It could just be my imagination, but I don't think it is.

Joe,

I just noticed that you've got a nl x haze and not a nl. My bad. I have a amnesia that's similar, but it's not high in linalool, it's sky high in limonene. You might be able to look it up to see what others are seeing.

WHEN YOU TRY PURE THC + THE RIGHT TERPENES THERE IS NO QUESTION IF IT WORKED.

 

[in vivo]

I don't believe there's any question when you simply put essential oils on top of flowers. The beta-caryophyllene is more subtle because it doesn't seem active in and of itself.

I have an amnesia that has been tested at over almost 4% between CBC and CBG which makes up over 12% of the cannabinoid profile. I also have a hindu kush that's been tested at over 8% CBG which makes up about 33% of the profile. The cannatonic (high CBD pheno) oil that I had tested showed everything including almost 1% delta8. They're all posted in the thread I linked.

If you look on the testing sites you can view the profiles and clearly see the presence of other cannabinoids, but I agree that they've practically been all but bred out.

The other interesting thing is that supposedly you can extract CBC from practically any high THC plant by harvesting the sessile trichomes in the vegetative stage. I've yet to do this, but I'm interested in it.

 

[Sam_Skunkman]

I don't believe there's any question when you simply put essential oils on top of flowers. The beta-caryophyllene is more subtle because it doesn't seem active in and of itself.

I have an amnesia that has been tested at over almost 4% between CBC and CBG which makes up over 12% of the cannabinoid profile. I also have a hindu kush that's been tested at over 8% CBG which makes up about 33% of the profile. The cannatonic (high CBD pheno) oil that I had tested showed everything including almost 1% delta8. They're all posted in the thread I linked.

If you look on the testing sites you can view the profiles and clearly see the presence of other cannabinoids, but I agree that they've practically been all but bred out.

The other interesting thing is that supposedly you can extract CBC from practically any high THC plant by harvesting the sessile trichomes in the vegetative stage. I've yet to do this, but I'm interested in it.

My earlier analysis's showed the Cannabinoid profile is pretty constant and does not change in qualitative profile during maturation just quantitative amounts. Either the CBC is there or not.
But this is newer then my older work. And done by someone who's work I respect. He used RJ97 for the work, that is a plant I know very well. And water sifting in some aspects, I know that pretty well.
I am not so sure the best way is to "extract CBC from practically any high THC plant by harvesting the sessile trichomes in the vegetative stage."
I still don't think you will get much CBC without keeping the plant vegetative and having at least genetic factors encoding prolonged juvenile chemotype (PJC),and it has a B0/B0 genotype.

How to do it:
a. Isolating/selecting a first plant comprising at least one genetic factor encoding prolonged juvenile chemotype (PJC);
b. Isolating/selecting a second plant comprising a B0/B0 genotype;
c. Crossing the first plant and second plant to obtain an F1; and
d. Self-fertilising selected F1 plants to obtain an F2 generation and selecting those plants with a high proportion of the cannabinoid CBC(A) relative to other cannabinoids.

See below:

SUMMARY OF THE INVENTION
According to a first aspect of the present invention there is provided a Cannabis sativa plant producing as its major cannabinoid CBC(A), characterised in that it comprises at least one genetic factor encoding prolonged juvenile chemotype (PJC) and it has a B0/B0 genotype.

In one embodiment the at least one genetic factor encoding prolonged juvenile chemotype (PJC) is monogenic.

The monogenic factor may derive from an Afghan lineage (CBD(A) dominant chemotype) such as, for example, that designated RJ97.11.

In an alternative embodiment the at least one genetic factor encoding prolonged juvenile chemotype (PJC) is polygenic.

The polygenic factor may derive from a Korean lineage (THC(A) dominant chemotype) such as, for example, that designated 2000.577.118.

Indeed, the Cannabis sativa plant may comprise a plurality of genetic factors encoding prolonged juvenile chemotype (PJC).

The Cannabis sativa plant additionally comprising a B0/B0 genotype, such as that derived from Italian fibre hemp, isolate ISCI529/72 (also referred to as 2001/25) or more preferably, from a Ukranian fibre hemp, such as isolate USO 31. This cultivar is amongst several varieties of hemp that have been approved for commercial cultivation under subsection 39(1) of the Industrial Hemp Regulations in Canada for the year 2007.

The Cannabis sativa plant phenotypically comprises leafy inflorescences with a few small bracteoles, and bracts that predominantly carried sessile glandular trichomes and substantially no stalked ones as illustrated in FIGS. 6 d-f.

Preferably, the Cannabis sativa plant comprises, at maturity, greater than 65%, though 70%, 75%, 80%, 85%, 90% and 95% by weight CBC(A) based on the total weight of cannabinoids and may comprise as much as 98% or more by weight CBC(A) based on the total weight of cannabinoids.

According to a fifth aspect of the present invention there is provided a method of deriving plants yielding a high proportion of the cannabinoid CBC(A) at the expense of other cannabinoids comprising:

a. Isolating/selecting a first plant comprising at least one genetic factor encoding prolonged juvenile chemotype (PJC);
b. Isolating/selecting a second plant comprising a B0/B0 genotype;
c. Crossing the first plant and second plant to obtain an F1; and
d. Self-fertilising selected F1 plants to obtain an F2 generation and selecting those plants with a high proportion of the cannabinoid CBC(A) relative to other cannabinoids.
According to a sixth aspect of the present invention there is provided methodology for cultivating plants such that they yield a high proportion of the cannabinoid CBC(A) at the expense of other cannabinoids comprising:

a. Growing the plants under a defined reduced light intensity, and/or
b. A defined reduced generative phase.
Light intensity can be defined by the level of photosynthetically active radiation PAR measured in W/m2 or cumulative PAR measured in MJ/m2. A reduced light intensity (for growing cannabis plants) would be less than 17.45 MJ/m2 or 67.4 W/m2

Typically a cannabis plant which is grown from cuttings is subject to 5 weeks (35 days) of vegetative growth (usually under 24 h light) and then 8 weeks (56 days) of generative growth (usually under 12 h light). Total 13 weeks (91 days).

A reduced generative phase is thus one of less than 8 weeks (from day 35), and may be measured in days or weeks. Preferably the reduced generative phase is less than 7 weeks, more preferably less than 6 weeks, more preferably still less than 5 weeks and most preferably about 4 weeks in length (See e.g. FIG. 3 d.)

According to a fifth aspect of the present invention there is provided a method of deriving plants yielding a high proportion of the cannabinoid CBC(A) at the expense of other cannabinoids comprising:

a. Isolating/selecting a first plant comprising at least one genetic factor encoding prolonged juvenile chemotype (PJC);
b. Isolating/selecting a second plant comprising a B0/B0 genotype;
c. Crossing the first plant and second plant to obtain an F1; and
d. Self-fertilising selected F1 plants to obtain an F2 generation and selecting those plants with a high proportion of the cannabinoid CBC(A) relative to other cannabinoids.
According to a sixth aspect of the present invention there is provided methodology for cultivating plants such that they yield a high proportion of the cannabinoid CBC(A) at the expense of other cannabinoids comprising:

a. Growing the plants under a defined reduced light intensity, and/or
b. A defined reduced generative phase.
Light intensity can be defined by the level of photosynthetically active radiation PAR measured in W/m2 or cumulative PAR measured in MJ/m2. A reduced light intensity (for growing cannabis plants) would be less than 17.45 MJ/m2 or 67.4 W/m2

Typically a cannabis plant which is grown from cuttings is subject to 5 weeks (35 days) of vegetative growth (usually under 24 h light) and then 8 weeks (56 days) of generative growth (usually under 12 h light). Total 13 weeks (91 days).

A reduced generative phase is thus one of less than 8 weeks (from day 35), and may be measured in days or weeks. Preferably the reduced generative phase is less than 7 weeks, more preferably less than 6 weeks, more preferably still less than 5 weeks and most preferably about 4 weeks in length (See e.g. FIG. 3 d.)

-SamS

 

[in vivo]

I understand where you're coming from. This is actually fairly interesting. Here's a thesis written by a guy with access to GW Pharma. He identifies the ability to procure CBC and/or CBG rich concentrates via bubble bags by filtering out the larger heads and capturing the 25 micron from plants in early veg. They did this test with 178 plants from a 'variety of sources' and found that CBC was the most prominent in 4.5% and second most prominent in 78%. I guess that makes me wrong then, maybe it's CBG that's easier to procure. Not long after this discovery GW Pharma patented this process for procuring CBC including further purification.

http://www.google.com/patents/US20110098348

http://www.scribd.com/doc/214031988...of-Cannabis-Sativa-l-as-a-Phytopharmaceutical

sessile trichomes, which have been shown to have a higher proportion of CBC to capitate stalked trichomes of the same age

from this test it was important to note that separating trichomes with sieves appeared to offer a means of producing phytopharmaceutical feedstocks with a favorably altered cannabinoid profiles

 

[Dropped Cat]

And all the other grows were they in soil?
If I select a clone by smell normally every time I flower the same clone the smells are just the same. Anything different in the later grows? It is not so easy to predict smells even with crosses or selfings, but I am really surprise the flowering smells just changed, I have not see this. I wish I could help you, glad you still like the Thunk clone. Is the maturity? Is the pepper smell from young or real mature live buds, or from the dry bud? I love a great THUNK!!!
-SamS

The original plant from seed was flowered in soil.

The smell came on virtually overnight. I believe
around the 60 day mark. So strong I laughed because
back then I had no idea cannabis could smell like
crushed black pepper!

The original cured 30 days and the pepper receded
to reveal some citrus and churchy scents. I still have
some of that stash, from February 2011. It's smooth,
but has lost some character. More of a novelty than anything
else. The more recent stash is obviously preferred.

The first cutting after reveg was in soil, no pepper.

Subsequent grows in coco hempys. I should note I grow
micro in 16 and 32 ounce pots under 150w. This thunk
flowers for 110 days minimum. I have let one go 130 days,
but that was just a what if.

The smell in flower since reveg is musky/hash with light
citrus comes up around 45-50 days. The cured stash smells
just like the original, minus pepper.

I rotate this thunk, that is to say I take cuttings,veg for
a few weeks, then take cuttings and flower the donor.

Maybe have done this 5 or 6 times.

And yes, I like the thunk, thanks again!

 

[Sam_Skunkman]

By any chance did you show the plant to a friend that was a chef just before it smelled like pepper?
Does it still smell a bit of pepper? Get it analyzed and then and see what the terpenes are? How long did the pepper smell plant flower? Were they different in size? The plants grown in soil?

I had a plant from seeds I collected in India back in the 70's that smelled just like Vic's vapo rub.
That has: camphor, menthol, spirits of turpentine, eucalyptus oil, cedar leaf oil, myristica oil, thymol.

Camphor 4.8% (Cough suppressant and topical analgesic)
Eucalyptol 1.2% (Cough suppressant)
Menthol 2.6% (Cough suppressant and topical analgesic)
Inactive Ingredients
Cedarleaf oil
Nutmeg oil
Thymol
Turpentine oil

It was a Sativa NLD variety grown in the ground, that gave me over 3 Lb back when people were getting 100 grams per plant, and it was really really strong and up, Rob Clarke loved it and drew a picture of a bud from it for Marijuana Botany. No clones back then so it is gone....
I can still smell the Camphor and Menthol...
Made me feel like a little kid again....
-SamS

The original plant from seed was flowered in soil.

The smell came on virtually overnight. I believe
around the 60 day mark. So strong I laughed because
back then I had no idea cannabis could smell like
crushed black pepper!

The original cured 30 days and the pepper receded
to reveal some citrus and churchy scents. I still have
some of that stash, from February 2011. It's smooth,
but has lost some character. More of a novelty than anything
else. The more recent stash is obviously preferred.

The first cutting after reveg was in soil, no pepper.

Subsequent grows in coco hempys. I should note I grow
micro in 16 and 32 ounce pots under 150w. This thunk
flowers for 110 days minimum. I have let one go 130 days,
but that was just a what if.

The smell in flower since reveg is musky/hash with light
citrus comes up around 45-50 days. The cured stash smells
just like the original, minus pepper.

I rotate this thunk, that is to say I take cuttings,veg for
a few weeks, then take cuttings and flower the donor.

Maybe have done this 5 or 6 times.

And yes, I like the thunk, thanks again!