Apple Fritter, Super Buff Cherry #26, Permanent Marker, Apples&Bananas - 32 Klone im Zelt - endless

[doc_loomis]

Sieht richtig nice aus. Ich schäle meine Steckies zwar nicht, aber ritze sie an, und habe auch immer das Gefühl, dass sie dadurch mehr und an allen Seiten austreiben und im Endeffekt stabileres Wurzelwerk bilden. #broscience #definitelymaybe

 

[Dr.Dutch]

#broscience #definitelymaybe

Hört sich hier ja nicht nur nach bro-science an. Mal gucken, wie die Ergebnisse jetzt ohne Chito in der NL werden.

Phenotypic plasticity influences the success of clonal propagation in industrial pharmaceutical Cannabis sativa

The burgeoning cannabis market requires evidence-based science such that farmers can quickly and efficiently generate new plants. In part, horticultural operations are limited by the success of cloning procedures. Here, we measured the role of environmental conditions and cultivar identity on...

journals.plos.org

[...]
Aus post 76 hier - hatte in die Studie noch gar nicht reingeguckt bis gerade - real science, no bro science

Does rooting vary among genotypes and environments?​

Cultivars differed significantly in the tendency for cut stems to produce roots and the speed with which they rooted (Table 2). Of the cultivars we tested, Ghost Train Haze rooted most frequently and most quickly (Fig 4). Stem wounding improved rooting success (Table 2) such that wounded plants were 162% more likely to root than unwounded stems and rooted 1.5 days earlier (Fig 4). There was no significant interaction of cultivar and wounding for the tendency to root (Table 2A); however, there was a significant interaction for days to rooting (Table 2B). Specifically, days to rooting for Headband was unresponsive to wounding treatment, whereas days to rooting was shortened for Bubba Kush and Ghost Train Haze when wounding was applied (Fig 4).

Wounding the Base Speeds Rooting By 160%​

“Wounding” by removing epidermal tissue from the bottom 5cm of a clone’s stem by scraping a scalpel parallel to the stem surface, caused clones to pop roots 1.5 days faster, and with a 160% increase in rooting success (see the data).

It is important to note, that while wounding was found to increase the rate of rooting, (the speed) the study did not measure the ability to root, which means that if the test groups had all been left alone indefinitely, they might have all had a 100% success rate. To measure the speed difference, the test was stopped at a certain point when most of them were rooted, but before they all rooted. At that point they were dissected and measured.

It is theorized that wounding largely acts to break the very formidable barrier formed by the cuticle and make pericycle cells (which initiate lateral root formation) more accessible to external applications of rooting hormone.

My own tests with wounding…​

I find that scraping the surface of the stem does work as mentioned here, but it also increases the chance of pathogen invasion compared to other types of wounding. After performing several sets of side-by-side tests and observing the root growth from the stem under a microscope, I’ve found that scraping creates a section with no growth in the center of the scraped area, and growth around the border of the scrape. If the scraping action is prolific enough to create overlapping wounds that cover the majority of the stem, I see growth appear only at the top of scraped area and not within or below the scraped zone. This indicates that the scraping technique itself is actually inhibiting root growth, but at the same time exposing the pericycle around the edges of the scrape. Based on this observation, the pericycle exposure is the important part. Root growth appearing only at the top of the scrape, indicates that the nutrient flow which fuels root growth comes down from the leaves. Root growth does not ever appear in the middle of the scraped off areas.

Scraping is very susceptible to human error, so that no two technicians will ever scrape to the same degree or depth, and even the same technician would have a hard time replicating a scraping action with any degree of accuracy. If slightly overdone, the scraping action removes the phloem and exposes the woody xylem of the clone. If the clone is allowed to run over 10 days, this large open wound will frequently become infected with a bacterial pathogen. This elevated rot probability seems to be associated with exposing a large open area to the growing medium without the protection normally provided by the cuticle.

In an effort to find a wounding method that provides the least exposure to pathogens, while still exposing the pericycle to the rooting medium, I’ve tried all sorts of wounding styles…

Circular and spiral cuts around the stalk produced roots in a ring above the wounded area and nothing below that point. It seems clear that the flow of plant fluids is stopped or inhibited by any wounding style that circles the stalk.

The same effect was caused by an abundance of shallow cuts all around the stem. Roots only formed above the cut area.

Split Stalks performed the worst of all methods attempted. For unknown reasons, if your wound divides the stalk completely through the middle to create two separate pieces, that cutting will not grow roots.

The magic combo is 3 lengthwise cuts spaced evenly around the stem and only deep enough to break through the thin phloem layer. With this pattern, there is ample undamaged area in the phloem to allow the unrestricted flow of nutrition coming down from the leaves. The thin opening made by a longitudinal razor blade cut is enough of a break in the cuticle to allow entry of the rooting gel, while not so big as to provide a large open surface wound for bacterial invasion. Prolific root growth bursts directly out of the full length of the cuts and rarely ever from the uncut cuticle.

Caution: Wounded areas must be set fully into the media. Wounds that are exposed above the media after the clone is set, become doorways for fungus invasion.

https://cannabiscultivationinstitute.com/cannabis-cloning-studies-reveal-surprises/

Darf ich fragen was du bei 7 Tage Wachstum an Ertrag an einer Pflanze hast? Bei mir waren es ja ein paar Tage mehr - wegen dem Lichtstress nicht optimale Bedingungen. An meiner PM waren 21 Gramm was ich für eine kleine Pflanze beachtlich finde. Da merkt man wie dicht die Buds sind.

700g/m² - 7 Pflanzen bei 16 auf 1,44m² = 7 Pflanzen auf 0,63m² = 441 / 7 = 61g je Plant ... Bei der AF, PM war ja minimal über 700, also auch minimal über 61g je plant.