Very interesting subject,,, tc applications in reference to auto flower genetics,,
Has it been established what the real trigger is,, hormone,, chemical,, auxin, etc,,, ratios?
Since ruderallis is a recessive trait, and limited time before plant expires (unlike photoperiods or annual) I've not heard of autos successfully being part of TC. Not to say it can't be done....I'll see what I can discover.
When you Google different forums from several years ago, people are excited to try with autos....but nothing definitive....posts fall off. There are no results I found. Will keep digging.
It's not as easy as it sounds....tissue culture.
The concept of synthetic seeds is what first attracted me to learn more about TC.
Well auxin/hormones is the morphogen than makes the plant flower but autos just start making them, ya know...automatically so nothing triggers flowering but age/time. Just like humans and adolescence/puberty.
Interestingly, transfusions of blood from young people into old people actually rejuvenates the recipient AND vice versa ie. old folks blood prematurely aged young recipients.
It would appear autos are like us... they have a genetic timer/clock.
The other side of the coin with this idea is that, in theory, if humans could adapt to be like photoperiodic plants, we could live indefinitely as kids as long as we get 18/6
They have drugs these days they are giving to kids to stop them developing sexually. It’s an evil practice that facilitates subsequent gender “reassignment” once the child is legally adult, and completely brainwashed.
Anyway, I wonder if such a drug could be found to “pause” the development of autoflowering plants?! Then you could keep auto mothers and make clones normally. You just stop giving the chemical when you want them to flower.
I'm wondering if a root or folar application of hormone inhibitors could prove the triggers
It's actually easy,, just difficult to maintain,, people can't just keep a sterile zone clean for extended periods of time,, that's why the bags are brilliant [URL=https://www.icmag.com/ic/picture.php?albumid=79105&pictureid=1921118&thumb=1]View Image[/url] [URL=https://www.icmag.com/ic/picture.php?albumid=79105&pictureid=1921117&thumb=1]View Image[/url] [URL=https://www.icmag.com/ic/picture.php?albumid=79105&pictureid=1921119&thumb=1]View Image[/url]
[URL=https://www.icmag.com/ic/picture.php?albumid=79105&pictureid=1920605&thumb=1]View Image[/url] [URL=https://www.icmag.com/ic/picture.php?albumid=79105&pictureid=1921117&thumb=1]View Image[/url] [URL=https://www.icmag.com/ic/picture.php?albumid=79105&pictureid=1921118&thumb=1]View Image[/url]
This is where the bag and the internal bags are a pretty big evolution on the game,, the blue bag is self sterilising with uvc,,it's an incubation chamber,, it packs away and pops up totally sterile
It's a problem solver,,
With that....go for it!!
mycotopia.net doesn't show any worthy info on autoflowers tissue culture.
Try it and report back.
We need to figure out the triggers,, if it's just a build up of hormone inside the tissue similar to rooting without stims,, then maybe a inhibitor could bring a true auto flower back to veg
If you forced calus culture from a leaf explant,, i don't see why you couldn't hold it in callus state,,,
Have you guys thought much about callus culture,, it's an important part of the kit,, it's called indirect oganogenisis