Who Are The Coconut Crew? #TCUK

[englishrick]

Indirect oganogenisis is callus culture BTW bro,, did you notice that?

 

[ReikoX]

I have a feeling its a hormone,, similar to what builds up in darkness when we are in 1212,,, i bet it just builds up in the tissue with autos and there is some sort of reuptake system that stops normal veging plants from flowering,, just a guess

This is exactly what my intuition tells me. Somehow this hormone is not getting destroyed with light like a photoperiod plant. Autos grow very similar to a plant put on 12/12 from seed (though still finish faster).

 

[englishrick]

This is exactly what my intuition tells me. Somehow this hormone is not getting destroyed with light like a photoperiod plant. Autos grow very similar to a plant put on 12/12 from seed (though still finish faster).

Yes,,, that makes alot of sense,,, so the theory for me is that the hormone is killed in normal veg plants but the autos somehow hold this hormone and In turn it builds up and flowers,, makes alot of sense,,, the reuptake model is far more believable to me than anything else

 

[ReikoX]

Yes,,, that makes alot of sense,,, so the theory for me is that the hormone is killed in normal veg plants but the autos somehow hold this hormone and In turn it builds up and flowers,, makes alot of sense,,, the reuptake model is far more believable to me than anything else

Interesting article about the subject.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903977/#!po=11.9048

Particularly this:

A grafting experiment in tobacco plants with different photoperiodic responses strongly supported this hypothesis; a floral inhibitor produced in the leaves of LD tobacco under non-inductive short day (SD) systemically inhibited the flowering of the grafted day-neutral (DN) tobacco plants (Lang et al. 1977).

 

[Spaventa]

A grafting experiment in tobacco plants with different photoperiodic responses strongly supported this hypothesis; a floral inhibitor produced in the leaves of LD tobacco under non-inductive short day (SD) systemically inhibited the flowering of the grafted day-neutral (DN) tobacco plants (Lang et al. 1977).

Nice find ReikoX. So you could graft some leaves from a photoperiod strain onto an auto to keep it as a mother.... the cuts from it would still be auto and flower once over the stress of rooting probably. A longer flowering auto would be best so the clones get big enough. Sounds doable. Yet more experiments to do!
The auto strain I’ve ordered to test flower inhibitors are quite fast so I might order a longer one for this grafting thing. It’s an exscuse to buy seeds if nothing else, right?!

 

[englishrick]

Very very very very very very interesting

 

[englishrick]

Do you think it's possible,,??

The flowering of rudaralis is (possibly) directly related to ethylene??? , plants sense ethylene in the environment by using protein receptors inside the cell,,, is it possible that autos just produce / store huge amounts of ethylene or have overactive receptors ????????? ,,

one way to inhibit the ethylene response is to occupy the receptors with a structurally similar but non-bioactive compound,,,

has anyone tried ethylene inhibitors or receptor plugs in relation to keeping autos in veg?

So you Could potentially do a root or folar application of an ethylene inhibitor or receptor plug to keep an auto in eternal colonial / multiplication mode

I propose this,,, you can induce callus state via leaf samples taken from late late late flowering plants,,let's say you just grew 100 and you want to reveg 1 prized indervidual ,, ,you could take a leaf sample or even a root tip and induce callus state,, maintain callus state by ballance of auxin : cytokinin,,, the multiplication callus mode give you the opportunity to "clone",, ,, ethylene inhibitors and receptor plugs can be used (potentially) to maintain veg state,,, problem solved????

This is all stuff allready in the kit,,, ethylene inhibitors in the form of nano Ag suspended in H2o is essential in my kit for making female seeds,, the ethylene itself is for turning males to female,, it's also used as a force floweing teck and potentially even a defolar tek,,, ethylene inhibitors should delay flower,, in conjunction with a receptor plug it might just stop autos from flowering and keep them in eternal colonial/multiplication mode,,

 

[skotty]

So what happens when you graft a branch from an auto flowering plant onto a photoperiod in veg?

Does the photoperiod plant automatically start flowering or does the auto flowering plant continue to veg?

Or

Does that autoflower plant continue to flower regardless of what its attached to and the photoperiod continues to veg

Also what would happen if the donor plant was a male and you grafted onto him instead of her??

You dont really need tissue culture to answer these questions?

By feeding autoflowers extra nitrogen into the 5/6th week you can pro-long flowering. the sooner you give them bloom ratio the quicker they will flower but ultimatley they have adapted to just go for it..

Personally i think the trigger response is either gonna be on or off..

Turn it off you get autoflowering turn it on you get photoperiod i dont think theirs any in between..

How long does a male autoflower live for and what would happen if you grafted auto fem onto auto him?

 

[Spaventa]

ER, the first tests I’m doing are on controlling that hormone balance. If I can, then that’s it. If I can keep any cannabis plant really auxin heavy, it won’t flower, at all, even on 12/12! Then you just Mum one up and your sorted. You control the hormones on the clones till you want them to flower and then let them go. If they are autos you could time it all for best weather, longest days, highest temps, whatever your location got, whenever that is.

Equally If you want a photoperiodic plant to flower early, regardless of day length, you tip the balance the other way, simples. Just need the right gear in their water

Skotty, you should have a go at the grafting mate, make some Frankenstein shit

If ER wants to try tissue culturing loads of clones of an auto, I’ll supply leaves from my test subjects.

I’ll stick to trying stuff in the res because my aero set up is the perfect controlled environment for that and between us I’m sure we will turn 1 auto into a thousand by June

Edit - This will elevate auto strains to a whole new level of usefulness and loads more people will by them.

 

[englishrick]

I'm too busy to do it myself atm,, I'm flat out doing editing, the website, the kit,, the talk,,, il have more time in May

 

[englishrick]

What auxin?,, what action are you talking about exactly?,,

you might find it an easy job to plug the receptors and/or inhibit ethylene,, it could be an simple nonbioactive soloution for ethylene enduced flowering

 

[Spaventa]

Ethylene doesn’t trigger flower, it ends it. It brings on senescence, or ripening. Cytokinins bring on flower.
If auxin levels outway cytokinins levels, the plant will veg. I’m looking at chemicals that might trigger production of auxins. I’m not looking to supply the hormones, just induce the plant to make its own.

 

[englishrick]

Remember we are talking cannabis specific so you can't rely on non canbabis papers,,, some plants litrally react totally different

Canna plants can be induced to flower by treatment with ethylene gas in the chamber,,, please check

I also read that ethylene interferes with auxin transport (with high auxin concentrations)

 

[Spaventa]

Remember we are talking cannabis specific so you can't rely on non canbabis papers,,, some plants litrally react totally different

Canna plants can be induced to flower by treatment with ethylene gas in the chamber,,, please check

I also read that ethylene interferes with auxin transport (with high auxin concentrations)

That’s fair comment. I’m only just starting so far from certain of anything specific.

I’m pretty sure GH Ripen has ethylene in it
and whilst it forces plants to finish, they don’t claim it forces flower.

All the same I have loads yet to learn so it’s another avenue to explore properly.

Right, back to tissue culture!

Here’s one for ya... IF I was able to get a barely dry bud of cheese (none of ya knockoffs) could you culture THAT back to clone??? If so, I know a fucker who punts it but don’t share the cut... can it be done? Cannnabis necromancy?! The Frankenstein grafting thing and now this lol

 

[Harry Lime]

I am hopefully going to be doing a full talk at Britain's got dank,, so by then the kit will be fully explained and detailed for the release of the kit

I'd like to hear this talk but what's Britain's Got Dank?

 

[englishrick]

It's an event,, i was just speaking to the guy who runs it,, it's a secret cup,

 

[Harry Lime]

So the talk will be in secret too? Shame. Maybe you could stick it in a podcast or similar.

 

[englishrick]

Secret yes,, quiet, no,, il be recording it so don't worry,, il put a lil camara in front of me,, remember I'm doing videos for YouTube and Instagram

 

[Badfishy1]

Rick- have been looking into tc and most Canna related info I find is on meristem propagation. Is there a way to go with an even smaller explant (like a fan leaf or say all the way down to a single cell)

 

[siftedunity]

The main and initial motivation on this kit was down to a couple of factors,, it was and still is entirely driven by the need for soloutions to problems we collectively face,,

The business is taking the form of a Cooperative,, iP is another thread,, the only IP applicable to this kit is specific formulations nothing genetic,, but that's not to say you can't go gm with the help of this kit,, you could,, iP is litrally not applicable to this thread unless we start talking gm,, to be fair this thread is actually quite the opposite of what private property laws attempt to uphold,, this is a group,, this is support,,it's almost a Marxist ideology,,, this is me personally upping people's game,, it takes effort and I give it with no expectations,, nothing in return, just respect and mutual interest,, please don't get twisted by people who babble into the abyss,,

The main motivation for creating this kit was to store huge germplasm librarys in a small space,, stasis for prolonged periods with little to no effort was the goal,, after digging into science papers I noticed potentially interesting tek,, when attempting to simplify I started to solve multiple problems,, eventually I thought "shit',, this is a game change,, il have to put this out,, i owe it to people ,,

this isn't new in the uk. in the usa its already becoming quite mainstream. and uk hobbists have been doing this with orchids etc for years. its just that most of the canna community doesn't look to scientific horticulture for anything related to growing weed. tissue culture kits are available online in the uk already, all you would really need is an autoclave or pressure cooker to start yourself up. but this kit will be nice for a newbie to start up before getting some better equipment.