growing cannabis inside, especially sativa landraces is a labor of love. it can be very frustrating especially to newbies hoping to supply their own stash. however hermies and seeds can discourage new growers invariably they either quit or adapt and learn. a big part of the lack in true sativa effect cannabis is due to the difficulty growing inside and the lack of climate to grow them outside. unless your breeding, a seeded crop is your worst nightmare.
even tho most people grow from clones seed plants ive found to be far superior in growth but unpredictable sexual stability. until a full run is done with the females, the stability is a complete mystery. only takes a couple bannanas to seed the whole op. we even see this outside with pollen drift from cbd hemp ruining cannabis crops. there is a very simple solution- create sterile lines! no seeds or pollinated pistils. this would revolutionize home growing further shifting the paradigm away from the corporate swine back to the passionate potheads. how to accomplish this?
example from landracemafia-`which is naturally occuring
how could this be accomplished? several methods but all involve chromosome mutations/number change. we are looking for females which cannot be pollinated so the only foolproof selection is to use the 3 methods and handpick females which are sterile but also have improved traits due to gene dosage changes affecting quantative traits.
option 1- hunt landraces like landrace mafia and maintain by clone- most time consuming, limited trait improvement. however it is the least complicated and shortest process
2. triploids- this method is used in the fruit industry like watermelon. accomplished by crossing a diploid plant to tetraploid. the progeny will be mostly triploid(2 +4=6/2=3) another way to produce a triploid is using endosperm rescue which is naturally triploid. endosperm is what the seed uses for nourishment before germinaton. since the tissue is naturally triploid, rescuing it from a nearly ripe seed is a short process with predictable triploid outcome but very technically advanced.
3. aneuploid swarm- best inhanced trait results, most diversity previously successful in various haze lines. aneuploidity is a chromosome mutation where the plant has extra chromosomes but not a full set.
for example a plant could have 22 chromosomes 1 copy each of 20 and then an extra copy of 2 and 4. this will throw off the genes balance leading to extreme phenotypes not observed in either parent. the traits changed will be specific to the quantative traits located on 2 and 4. these are called karyotypes which describe how many chromosomes, which are extra and the associated morphology/secondary metabolite phenotypes
to create aneuploids you have 2 options- self seed a triploid or cross a diploid to a triploid. these changes in chromosome count are caused by the complicated and difficult meiosis that triploids go thru. since there are 3 sets of chromosomes but only 2 poles alot of unequal distribution occurs. the results of these triploid crosses is called a hybrid aneuploid swarm and it will contain diploids, triploids, aneuploids of varying karyotype and even some tetraploids. this type of mutation also occurs naturally in hybrid zones where several ploidities are present.
anybody run into a sterile female before?? closest production clone now would be mac1 which is a triploid. this comes from the colombian "miracle" parent. it most likely went thru chromosome doubling due to unreduced gametes in the challenging enviorment or spontaneous doubling due to elements of challenging enviorment(i think all the colombian plants died but this one hence the "miracle" while mac1 is not completly sterile it has difficulty being pollinated and performing reversals.
4. mutated pollen. same idea to change chromosome arrangement but a different tactic. less predictable then karyotypes but possibly more diverse. radiating the donor pollen will cause deletions, additions and even broken chromosomes. however this would probly be the highest rate of sterile females due to the massive damage caused to pollen by radiation. also worth mentioning that aneuplooid swarm progeny can also throw out different ploidity pollen grains visibly different sizes.
even tho most people grow from clones seed plants ive found to be far superior in growth but unpredictable sexual stability. until a full run is done with the females, the stability is a complete mystery. only takes a couple bannanas to seed the whole op. we even see this outside with pollen drift from cbd hemp ruining cannabis crops. there is a very simple solution- create sterile lines! no seeds or pollinated pistils. this would revolutionize home growing further shifting the paradigm away from the corporate swine back to the passionate potheads. how to accomplish this?
example from landracemafia-`which is naturally occuring
how could this be accomplished? several methods but all involve chromosome mutations/number change. we are looking for females which cannot be pollinated so the only foolproof selection is to use the 3 methods and handpick females which are sterile but also have improved traits due to gene dosage changes affecting quantative traits.
option 1- hunt landraces like landrace mafia and maintain by clone- most time consuming, limited trait improvement. however it is the least complicated and shortest process
2. triploids- this method is used in the fruit industry like watermelon. accomplished by crossing a diploid plant to tetraploid. the progeny will be mostly triploid(2 +4=6/2=3) another way to produce a triploid is using endosperm rescue which is naturally triploid. endosperm is what the seed uses for nourishment before germinaton. since the tissue is naturally triploid, rescuing it from a nearly ripe seed is a short process with predictable triploid outcome but very technically advanced.
3. aneuploid swarm- best inhanced trait results, most diversity previously successful in various haze lines. aneuploidity is a chromosome mutation where the plant has extra chromosomes but not a full set.
for example a plant could have 22 chromosomes 1 copy each of 20 and then an extra copy of 2 and 4. this will throw off the genes balance leading to extreme phenotypes not observed in either parent. the traits changed will be specific to the quantative traits located on 2 and 4. these are called karyotypes which describe how many chromosomes, which are extra and the associated morphology/secondary metabolite phenotypes
to create aneuploids you have 2 options- self seed a triploid or cross a diploid to a triploid. these changes in chromosome count are caused by the complicated and difficult meiosis that triploids go thru. since there are 3 sets of chromosomes but only 2 poles alot of unequal distribution occurs. the results of these triploid crosses is called a hybrid aneuploid swarm and it will contain diploids, triploids, aneuploids of varying karyotype and even some tetraploids. this type of mutation also occurs naturally in hybrid zones where several ploidities are present.
anybody run into a sterile female before?? closest production clone now would be mac1 which is a triploid. this comes from the colombian "miracle" parent. it most likely went thru chromosome doubling due to unreduced gametes in the challenging enviorment or spontaneous doubling due to elements of challenging enviorment(i think all the colombian plants died but this one hence the "miracle" while mac1 is not completly sterile it has difficulty being pollinated and performing reversals.
4. mutated pollen. same idea to change chromosome arrangement but a different tactic. less predictable then karyotypes but possibly more diverse. radiating the donor pollen will cause deletions, additions and even broken chromosomes. however this would probly be the highest rate of sterile females due to the massive damage caused to pollen by radiation. also worth mentioning that aneuplooid swarm progeny can also throw out different ploidity pollen grains visibly different sizes.
