RECENT interesting findings

[Brother Nature]

I love how this is a plant we've been breeding and manipulating for hundreds of years, if not more, in order to serve our needs yet we've only just recently started taking notes.

 

[Illuminate]

^^^sigged.

 

[Sam_Skunkman]

I added #299 & 277 at the bottom. Just drop the DOI number into sci-hub.cc and find them all complete.
Enjoy!!!
-SamS

Introduction to the Special Issue on Cannabis
Dennis J. Gray, Robert C. Clarke & Robert N. Trigiano
Critical Reviews in Plant Sciences,
DOI: 10.1080/07352689.2016.1267492


Current and Future Needs and Applications for Cannabis
Dennis J. Gray, Hailie Baker, Kayla Clancy, Robert C. Clarke, Kymron
deCesare, John Fike, Matthew J. Gibbs, Franjo Grotenhermen, Nolan C. Kane,
Kyle G. Keepers, Donald P. Land, Ryan C. Lynch, J. Paul Mendieta, Mark
Merlin, Kirsten Müller-Vahl, Christopher S. Pauli, Brian J. Pearson, Bailey
Rhan, Travis C. Ruthenberg, C. J. Schwartz,
Critical Reviews in Plant Sciences
DOI: 10.1080/07352689.2017.1284529



Genetic and Genomic Tools for Cannabis sativa
Daniela Vergara, Halie Baker, Kayla Clancy, Kyle G. Keepers, J. Paul Mendieta, Christopher S. Pauli, Silas B. Tittes, Kristin H. White & Nolan C. Kane
Critical Reviews in Plant Sciences,
DOI:10.1080/07352689.2016.1267496


Industrial Hemp: Renewed Opportunities for an Ancient Crop,
John Fike
Critical Reviews in Plant Sciences,
DOI: 10.1080/07352689.2016.1257842


Medicinal Uses of Marijuana and Cannabinoids
Franjo Grotenhermen Dr. med. & Kirsten Müller-Vahl
Critical Reviews in Plant Sciences,
DOI:10.1080/07352689.2016.1265360


The Derivation of Modern Cannabis Varieties
Bailey Rahn, Brian J. Pearson, Robert N. Trigiano & Dennis J. Gray
Critical Reviews in Plant Sciences,
DOI:10.1080/07352689.2016.1273626


Cannabis Domestication, Breeding History, Present-day Genetic Diversity, and Future Prospects
Robert C. Clarke & Mark D. Merlin
Critical Reviews in Plant Sciences
Doi: 10.1080/07352689.2016.1267498


Genomic and Chemical Diversity in Cannabis
Ryan C. Lynch, Daniela Vergara, Silas Tittes, Kristin White, C. J. Schwartz, Matthew J. Gibbs, Travis C. Ruthenburg, Kymron deCesare, Donald P. Land, Nolan C. Kane
Doi: 10.1080/07352689.2016.1265363

 

[emeraldcityog]

Holy shit, I didn't realize these were complete. Thank you!

 

[Sam_Skunkman]

Characterizing the Smell of Marijuana by Odor Impact of Volatile Compounds: An Application of Simultaneous Chemical and Sensory Analysis
Somchai Rice, Jacek A. Koziel
doi:10.1371/journal.pone.0144160

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144160

 

[Londinium]

Fascinating paper /\ thanks

 

[Sam_Skunkman]

I found pure THCV does not get me high. I did not like the effect of THCV/THC I find this similar to what happens with CBD/THC, it delays THC's onset, reduces peak experiences, and make the reduced effects last longer.
I look forward to many more studies with many more different Cannabinoids, we have a lot to learn. Medical uses of many different Cannabinoids is what I expect. For recreational, I do not want them, THC and terpenes are my goal.
-SamS


The effect of five day dosing with THCV on THC-induced cognitive, psychological and physiological effects in healthy male human volunteers: a placebo-controlled, double-blind, crossover pilot trial.
Amir Englund, Zerrin Atakan, Aleksandra Kralj, Nigel Tunstall, Robin Murray and Paul Morrison
DOI: 10.1177/0269881115615104

Conclusion
In this small pilot study with healthy infrequent cannabis users, results indicate that the dose of 10mg oral THCV is well tolerated with no serious adverse reactions, and was subjectively not distinguishable from placebo. Furthermore, the lower dose of 1mg iv THC did not produce any significant short-term memory impairment, or psychotic or paranoid reactions. THCV significantly inhibited THC-induced impairment to delayed recall as well as THC-induced increase of heart rate. THCV on its own showed signs towards improved performance on the harder working-memory task, while also producing a slight increase in
anxiety. However, these effects were small and need to be further studied in a larger sample.

 

[Gr33nSanta]

Thanks Sam, but this article was too short! What about THCV being an appetitite suppressant ?

Just like CBD, the minute I heard the word THCV I became obsessed and had to grow it/ find it.

I dont have a lab to test, but 2 varieties I was growing at a time were known to be high in THCV, blue city diesel, and jack the ripper. It was around this time last year that I ordered Chupacabra which could also test high THCV.

So far I found that the female chupacabra I kept is very mild, which was contradictory to the first bit I read about THCV which was supposed to create the no ceiling high effects.

Anyway, if my chupacabra is indeed high in THCV, Id have to agree with you that it feels CBDish.

EDIT sorry I found the full article. I was googling the DOI instead of going to sci-hub.cc. got some reading to do now Cheers

 

[numberguy]

If THCV and CBD delay onset, does THC alone cause instant onset or is there something else that can make it quicker?

 

[Sam_Skunkman]

If THCV and CBD delay onset, does THC alone cause instant onset or is there something else that can make it quicker?

The right terpenes can.
-SamS

 

[G.O. Joe]

Identification of Terpenoid Chemotypes Among High (−)-trans-Δ9- Tetrahydrocannabinol-Producing Cannabis sativa L. Cultivars
Fischedick Justin T.
Cannabis and Cannabinoid Research. March 2017, 2(1): 34-47.
http://dx.doi.org/10.1089/can.2016.0040

Introduction: With laws changing around the world regarding the legal status of Cannabis sativa (cannabis) it is important to develop objective classification systems that help explain the chemical variation found among various cultivars. Currently cannabis cultivars are named using obscure and inconsistent nomenclature. Terpenoids, responsible for the aroma of cannabis, are a useful group of compounds for distinguishing cannabis cultivars with similar cannabinoid content.

Methods: In this study we analyzed terpenoid content of cannabis samples obtained from a single medical cannabis dispensary in California over the course of a year. Terpenoids were quantified by gas chromatography with flame ionization detection and peak identification was confirmed with gas chromatography mass spectrometry. Quantitative data from 16 major terpenoids were analyzed using hierarchical clustering analysis (HCA), principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA).

Results: A total of 233 samples representing 30 cultivars were used to develop a classification scheme based on quantitative data, HCA, PCA, and OPLS-DA. Initially cultivars were divided into five major groups, which were subdivided into 13 classes based on differences in terpenoid profile. Different classification models were compared with PLS-DA and found to perform best when many representative samples of a particular class were included.

Conclusion: A hierarchy of terpenoid chemotypes was observed in the data set. Some cultivars fit into distinct chemotypes, whereas others seemed to represent a continuum of chemotypes. This study has demonstrated an approach to classifying cannabis cultivars based on terpenoid profile.

 

[Sam_Skunkman]

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0173911

Terpene synthases from Cannabis sativa
Judith K. Booth, Jonathan E. Page, Jörg Bohlmann
Published: March 29, 2017http://dx.doi.org/10.1371/journal.pone.017391



I liked this paper because I bred FINOLA, will be interesting when they do the same with more Cannabis varieties that yield closer to 10% terpenes, instead of the normal 1-2% terpenes found in most. They also show work with Skunk #1 and Purple Kush, I did not breed Purple Kush, I think I did all the others. Breeding for terpene profiles and %'s will be the future, work like this a very small step in that direction.
-SamS

 

[Nirrity]

SamS, did you find if any environmental factor can increase terpenes' content?

 

[angelgoob]

They say increased light can do that. I forget the source though.

 

[Nirrity]

angelgoob, never heard of light intensity plays role, nice to know.
there has been report on fs+red leds vs hps with clear limonene spike from leds, so i assume full spectrum does not only increase overall terpenes content, but what's more important make it fuller.
since monoterpenes are quite volatile perhaps grow space temerature, vpd/humidity, lower nutrients or who knows what else can increase terpene production.

would be nice to hear from Sam.

 

[maximinio]

Love these data!
Where do these come from?

 

[Nirrity]

it's from tags420 from thc farmer thread name spektrumkings led something

 

[Stan G.]

Wow! Sam, thanks for these articles. Makes interesting reading material when not chasing terpenes.

 

[Sam_Skunkman]

I have never seen an identical clone produce such different terpenes regardless of the light sources. Lights, sunlight, temps, soil, nutrients, humidity all affect the terpene profiles but I do not see new or different terpenes with the different environmental factors it does alter the ratios but not eliminate any or create new ones not found under the other conditions. I do not get it, to be honest.
-SamS

angelgoob, never heard of light intensity plays role, nice to know.
there has been report on fs+red leds vs hps with clear limonene spike from leds, so i assume full spectrum does not only increase overall terpenes content, but what's more important make it fuller.
since monoterpenes are quite volatile perhaps grow space temerature, vpd/humidity, lower nutrients or who knows what else can increase terpene production.

would be nice to hear from Sam.

 

[Sativied]

That appears to be greengenes' test result. No controls, no consistency in growing, no repeat results, could be a lot of other things. On the other hand, I would not be surprised at all if environmental influences can change the ratios to a point where one is not detected. Unless you can reproduce the results it's rather meaningless.

There is plenty of research that shows certain light recipes and intensity as well as nutrient availability heavily influence secondary metabolites production.