I think the people that needed to see that information were paying attention at the time. Thanks again Tom for taking the time to spread the knowledge/information. I particularly enjoyed the exchange with GMT. That, along with the link to ebay, was pure gold. (Note: Tom advised using a ratio of 3:9 rather than the 1:9 that is recommended by BudResearch.com).
M.
"if you start out with a set goal in mind and go after just that one thing then your eventually are going to be lacking in another area."
there is no way to understand what your intent is when on one hand you want zero phenos yet you say this ^.
ime, if you are selecting something youre choosing from a personalized set of criteria that has no choice but be different from one seedmaker to another. after growing a plant from seed i feel a level of proprietorship towards the future of its being in my care. having grown a few plants of diverse lineage i can get a phenotypical map of crude sorts and i recognize these different qualities in the various crosses going around and from there i am seeing the way theyre combing having made a bunch of polyhybrids myself. i love polyhybrids lol. i love the variety. freaks inc. hehe. a good breeder to me is someone who grows and smokes and evaluates a variety even up to handing out smoke report check lists to folks hehe. and they do this with as many different variety as they can working backwards into say a cross they like alot like blueberry say, and they decide they wanna check out chocolate thai. then they hear a cut of juicy fruit thai is around. then they start getting into SOL stuff where one frosty bb male was used in many varied crosses. you can see the "blue" influenced by that male distinctly in Steves crosses and bx's where he back crossed with the same male. the grapefruit clone was used in a couple crosses and now that ive toke a few haze crosses i can tell you i believe the sweetskunk clone is actually gfxnl/haze. this can only come from growing the actual plants for years. nevils haze is from 2 males and one female. i think his is actually just the males. i can't remember but i got it in my notes somewhere. this is alot less than we're led to believe by his saying he starts with thousands. he did, but only 3-4 popped, after he used gibberlic acid i think, im not exact here. i love his haze tho, i got decent plants in mns nevsf3 6/6 2 males 4 females. all pretty nice with the usual sandal wood, spice, citrus. one male threw pistils and was killed, the other is being used in various chucks. i grew a cpl s1 chem91 and they were like the mom. one thing i found in the butterscotch plants is that many of the males- and in crosses using a male butterscotch- produce pistils. i just had a [(chem91xbutterscotch)x(pre'88g13/hashplant)]xbutterscotch male reverse under stress and throw pistils resulting in 2 seeds. maybe oneday i'll get a chance to open those. ime there isn't enough time to be speculating about what a cross is going to be like before i do it because i'm very busy trying to catalog and experience the "pheno" i'm growing atm. cannabis is good all mixed up, it'll sort itself with a little help from us conscientiously or accidentaly. i think it matters if a person evaluating with their senses, is a cigarette smoker or a patient on pharma medications. opiates alter tastes like brushing ones teeth would before "smoking to evaluate". if this mundane "scratch and sniff" is done well and can be coupled with labratory testing and then these findings can be compared to others done on the same variety etc building a data base because no one person is going to be able to do this with any accuracy. team work and a desire to move forward would need to be the guiding force so that individual personalities wont interfere too much in the process of sharing our findings.
pie in the sky when capitalism trumps science.
i like my methods.
peace,
pwf
PWF:
Punctuation, bro. Not sure how many peeps labored through your post. I sure as hell didn't.
Friend in deed @ post #38:
Yeah, Tom, Chimera, SamS, GW Pharma & every other crop seed supplier watching MMJ news. Minus GC/LC testing: Charlie/Kaiki, Dubi, myself, Verdant Green, probably a some others.
@ICMAG community:
FWIW:
Tom is the most helping Breeder on these boards. This community comes here and asks for help, insights, etc. then when it's laid out for them they jump his shit cuz it's not what they want to hear. Maybe he seems opinionated but, he's the only breeder who doesn't currently sidestep that shit-storm! I for one am greatful for learning what I have over the years by searching for and reading his and others posts. It's helped fill in gaps left from reading other breeding related literature. I've had a lot of "aha!" moments studying multiple sources.
Maybe we should be asking why versus touting why not?
in the end it must come down to INTENSIVE progeny testing with huge # (100s of thousands and more)... hence it is nearly impossible for canna breeders to do real breeding when it comes to achieving the set breeding programm goals (think about how many folks would be needed to at least document the physiological differences amongst the phenos?), next step would be evaluating the effect/flavor/etc... as well as having to deal with traits which are percieved very subjectivly (taste, effect)...
hence the easiest and most prevelant/common route is pollenchucking, hoping for "bag appeal"/high resin ammount/stank/etc... and if those traits ain't there, just give it a crazy name and hope rappers will jump on the bandwagon
blessss
Here's the straight dope....
this whole IBL tag that popped up for marketing purposes a few years back did nothing but make a whole generation of newbs and wannabe breeders more ignorant, it set them about chasing a wagon that was heading squarely down the wrong path.
IBL was intended to denote InBred Line. How you inbred the line was moot, the point being that the line underwent intense selection for a specific set of traits.
The absolute fastest and tightest methodology for said task is the self-cross. The problem is that any trait that is the result of a heterozygous gene condition at a specific locus, falls apart in 50% of the progeny. Sure, it may also re-occur in %50 of the resulting as well, but it is by no means stable in those individuals, it's simply present.
The only way to recapitulate the hybrid (Aa) at that locus, is to take the 2x (25% classes, AA and aa) and intermate them to get the desired Aa genetic condition again.
A perfect example of this is the condition that leads to a mixed THC:CBD chemotype; if you self cross a THC:CBD individual, 25% will be CBD 'pure', 50% will recapitulate the hybrid condition (THC:CBD), and a further 25% will be THC 'pure'.
What I'm really getting at here is to back up Tom on a specific point, that the most reliable method for fixing a trait in a line is to cross to a plant that is a KNOWN carrier for that trait, IE- itself. If you were to chose a male sibling from the same population, there is no guarantee that such a male would also possess the same genetic condition that resulted in the trait appearing in your female. However, you KNOW the female possesses said trait, therefor mating the plant to itself is the surefire'est way to re-enforce the trait in the following generation.
The major drawback of this methodology is that you also fix undesirable traits in the line, at a rate of an increase of %50 homozygosity at every generation, therefor you require a serious selection pool to maintain your traits of interest, but purge the traits that are undesirable to the line. While I agree with Tom that the degree to shich you will see issues is very much attributed by the genetic burden hidden within the line, because at this point most cannabis individuals and lines, contain a relatively large genetic burden of undesirable alleles due to the obligate outcrossing nature of the species (coupled with the haphazard mating schemes she's been forced into for decades).
Yes, genetic gain is increased with respect to a very specific locus with each self-cross, but there is also a significant genetic burden placed on populations derived from such pool, to the increasing homozygosity of undesirable alleles @ 50% increase per generation. This is where Tom's families #'d 1-5 come into play... and while 1-5 is simply a number to demonstrate a point, I would suggest that %5 of 500 would be more appropriate to create these families, rather than say 5% of 50 or 100.
This is where IMO lab testing becomes truly advantageous- individuals can be scored on a whole range of traits, like cannabinoid and terpene profile, and evaluated on these characters alone to see if they fit the bill for further selfing or inclusion in the breeding regimen. If you don't have your own lab, this can get really expensive... because it's what we call a "brute force" screening method... it takes a pretty hefty resource budget to be able to screen hundreds of individuals by GC / LC... and a cost which most aren't interested in bearing when suckers line up to toss benjamins at you at trade shows because you told them your OG-blahblah was 30% THC (LOL!).
While you can make some progress SLOWLY by using the methods the naysayers and science-shunning types (from the Everybody a breeder thread) purport, you simply can't achieve the genetic gains associated with proper 'learned' scientific breeding methodologies coupled with solid analytical tools, which again is simply a fact in my world... having access to both methods. To paraphrase our old friend pnwhyb, we've made the same hack crosses years ago, we know it leads to dead ends compared to more appropriate, accepted breeding methods.
It's not just about selfing for homozygosity, it's about knowing what you are doing, understanding all the genetic possibilities, paying attention to phenoypes (chemotypes), and having the skills to read the cues that the genetics are telling you, by following traits/characters and spotting known accepted patterns (1:2:1, 9:3:3:1) when and where they arrise. If you make use of all this information, you'll be much further ahead of the curve than if you simply follow the 'I like this smell, it makes me feel good' breeding methods espoused by others. I can't emphasize enough that these types of insights are not precluded by developing an understanding of genetics, selection methodologies etc... you can still evaluate plants based on your chosen set of criteria and personal tastes, science doesn't take any of that away... much to the dismay of those trying to shun it as a useful tool in understanding just what the fuck we are trying to do.
-Chimera
Breeding flowering plants is mostly about luck.
Therefore the best breeders are the ones with the biggest populations, which plays towards a lucky find, a lucky cross which gives relatively high incidence of desired traits.
It's more about NOT distributing seeds, being strict with selection, testing huge numbers of crosses and re-making the best ones for bulking up seed pools.
IMO intensive breeding is a cheap shortcut and its failure is measured in the vast amounts of sick/weak lines which must be ended. If you instead breed less intensively, you can still go forwards towards enriching your traits, seeing which crosses turned out best and bulking them up, with the right kind of variation around those traits.
Understanding ratios of simplistic inheritance actually means very little and certainly is less valuable than trying more kin crosses out to check for better parent plants.
So for all the angry folk who really need to blame someone for a perceived decline, please aim it at intensive indoor breeders who talk about homozygosity as though it were desirable or possible, because it is neither.
And aim it at yourselves for not demonstrating how you think it should be done instead.
breeding weed is like breeding dogs , its like breeding anything, all the laws are the same. people that bred dogs didnt grow out thousands of dogs to get the dogs they wanted, they did it few dogs at a time, few litters at a time. yall guys are fuckin nuts. check out the story of dobermans. anyway im not a pro, i just know it aint as complicated as yall act, people been breeding flowers cats, dogs, veggies for thousands of years with no confusion, they know what they like and they try to make more !
http://en.wikipedia.org/wiki/Dog_breeding
No one shunned the use of science I just called Tom out on claiming it is the absolute difference between success and failure.Here's the straight dope....
this whole IBL tag that popped up for marketing purposes a few years back did nothing but make a whole generation of newbs and wannabe breeders more ignorant, it set them about chasing a wagon that was heading squarely down the wrong path.
IBL was intended to denote InBred Line. How you inbred the line was moot, the point being that the line underwent intense selection for a specific set of traits.
The absolute fastest and tightest methodology for said task is the self-cross. The problem is that any trait that is the result of a heterozygous gene condition at a specific locus, falls apart in 50% of the progeny. Sure, it may also re-occur in %50 of the resulting as well, but it is by no means stable in those individuals, it's simply present.
The only way to recapitulate the hybrid (Aa) at that locus, is to take the 2x (25% classes, AA and aa) and intermate them to get the desired Aa genetic condition again.
A perfect example of this is the condition that leads to a mixed THC:CBD chemotype; if you self cross a THC:CBD individual, 25% will be CBD 'pure', 50% will recapitulate the hybrid condition (THC:CBD), and a further 25% will be THC 'pure'.
What I'm really getting at here is to back up Tom on a specific point, that the most reliable method for fixing a trait in a line is to cross to a plant that is a KNOWN carrier for that trait, IE- itself. If you were to chose a male sibling from the same population, there is no guarantee that such a male would also possess the same genetic condition that resulted in the trait appearing in your female. However, you KNOW the female possesses said trait, therefor mating the plant to itself is the surefire'est way to re-enforce the trait in the following generation.
The major drawback of this methodology is that you also fix undesirable traits in the line, at a rate of an increase of %50 homozygosity at every generation, therefor you require a serious selection pool to maintain your traits of interest, but purge the traits that are undesirable to the line. While I agree with Tom that the degree to shich you will see issues is very much attributed by the genetic burden hidden within the line, because at this point most cannabis individuals and lines, contain a relatively large genetic burden of undesirable alleles due to the obligate outcrossing nature of the species (coupled with the haphazard mating schemes she's been forced into for decades).
Yes, genetic gain is increased with respect to a very specific locus with each self-cross, but there is also a significant genetic burden placed on populations derived from such pool, to the increasing homozygosity of undesirable alleles @ 50% increase per generation. This is where Tom's families #'d 1-5 come into play... and while 1-5 is simply a number to demonstrate a point, I would suggest that %5 of 500 would be more appropriate to create these families, rather than say 5% of 50 or 100.
This is where IMO lab testing becomes truly advantageous- individuals can be scored on a whole range of traits, like cannabinoid and terpene profile, and evaluated on these characters alone to see if they fit the bill for further selfing or inclusion in the breeding regimen. If you don't have your own lab, this can get really expensive... because it's what we call a "brute force" screening method... it takes a pretty hefty resource budget to be able to screen hundreds of individuals by GC / LC... and a cost which most aren't interested in bearing when suckers line up to toss benjamins at you at trade shows because you told them your OG-blahblah was 30% THC (LOL!).
While you can make some progress SLOWLY by using the methods the naysayers and science-shunning types (from the Everybody a breeder thread) purport, you simply can't achieve the genetic gains associated with proper 'learned' scientific breeding methodologies coupled with solid analytical tools, which again is simply a fact in my world... having access to both methods. To paraphrase our old friend pnwhyb, we've made the same hack crosses years ago, we know it leads to dead ends compared to more appropriate, accepted breeding methods.
It's not just about selfing for homozygosity, it's about knowing what you are doing, understanding all the genetic possibilities, paying attention to phenoypes (chemotypes), and having the skills to read the cues that the genetics are telling you, by following traits/characters and spotting known accepted patterns (1:2:1, 9:3:3:1) when and where they arrise. If you make use of all this information, you'll be much further ahead of the curve than if you simply follow the 'I like this smell, it makes me feel good' breeding methods espoused by others. I can't emphasize enough that these types of insights are not precluded by developing an understanding of genetics, selection methodologies etc... you can still evaluate plants based on your chosen set of criteria and personal tastes, science doesn't take any of that away... much to the dismay of those trying to shun it as a useful tool in understanding just what the fuck we are trying to do.
-Chimera
No offense but this is not dogs. Some are trying to find the right combination of cannabinoids for they're particular needs. Peace and thanks to all the breeders