Found my long lost seed collection

[Katsu]

Looks like I'm getting some tails. I'm cautiously optimistic. Ended up using rubber plugs because I was concerned about burning the tap root with soil. I stuck another batch in a temp controlled jar with some nutes and hormones for about 5 days, going to stick those in some plugs today. If the plug thing doesn't work, I'll use light soil next time.

 

[Hundred Gram Oz]

Nice one bro, sounds like the green thumbs are busting to get back in action again. Did ya scuff the beans?

 

[neongreen]

Shedding the shell/membrane is usually the main stumbling block with old seeds. If you do use soil try and plant ~1/2 inch deep and keep the soil surface moist - that kind of depth should give the seedling a better chance of shedding it's shell/membrane when it works it's way up. Without the moisture the membrane won't split, and the cotyledons won't be able to push the shell off.

If you do get any coming up with their shells on, mist them (use a suffocant/wetting agent), get a dome on them to increase the humidity and soften the membrane, and they should shed on their own. If not you can try micro-surgery. Just make sure the membrane is soft before you do. If you use a dome, once the shells are off, gradually acclimatize them to the dry air by raising the dome(to leave a larger gap between dome and soil) a little more each day.

 

[apeshot]

The best way I've found if a seed has popped with it's shell still on, is to mist with water from a spray bottle. Leave it for a couple of minutes and you will generally notice that the shell has opened up, ready to be gently picked off. Same for if the membrane remains attached.

 

[The Apprentice]

What a collection!
Good luck brotha man

 

[Cuzin_Dave]

I was house cleaning this past weekend and found some old Bros. Grimm beans from Heaven's Stairway circa start of the new millennium. One of those frozen in time gifts. I gave away virtually all my spare beans and breeding and stabilisation efforts to ingrates and hacks regrettably so. Haven't popped a bean in ages here. I'm hoping at least a few want to peep out of their shell. I have Cinderella, Apollo 11,Rosetta Stone and Sugar Blossoms soaking right now. Got two old packs of Apollo 13 with ctg on them and one pack of Grimm White Widow waiting their turn if none of the current ones make it.

 

[Scrappy-doo]

I'm looking for ways to germinate some stubborn seeds and came across this.

https://m.youtube.com/watch?v=uLRWrjy8UGg

Basically a tissue culture kit adapted for old seed germination.

Don't think I'll be buying it but should be easy enough to do a diy version. Looks pretty simple. Some interesting info at least.

 

[Cannavore]

Popping Old Marijuana Seeds with GA3
[YOUTUBEIF]tehCb0Zbi1M[/YOUTUBEIF]

http://www.ebay.com/itm/Gibberellic...nt-Growth-Hormone-international-/322015376039


Effects of Gibberellic Acid



1) Overcoming dormancy. Treatment with high concentrations of GA is effective in overcoming dormancy and causing rapid germination of seed. Concentrations of about 2 ppm can cause tubers to sprout earlier.

2) Premature flowering. If a plant is sufficiently developed, premature flowering may be induced by direct application of GA to young plants. This action is not sustained and treatment may have to be repeated. Formation of male flowers is generally promoted by concentrations of 10 to 200 ppm., female flowers by concentrations of 200 to 300 ppm. Concentrations of more than 600 ppm markedly suppresses initiation of both male and female flowers.

3) Increased fruit set. When there is difficulty with fruit set because of incomplete pollination, GA may be effectively used to increase fruit set. The resulting fruit maybe partially or entirely seedless. GA has increased the total yield in greenhouse tomato crops both as a result of increased fruit set and more rapid growth of the fruit.

4) Hybridizing. Pollination within self-incompatible clones and between closely related species may some times be forced by the application of GA and cytokinin to the blooms at the time of hand pollination.

5) Increased growth. GA applied near the terminal bud of trees may increase the rate of growth by stimulating more or less constant growth during the season. In a Department of Agriculture experiment, the GA was applied as a 1% paste in a band around the terminal bud of trees. Treatment was repeated three times during the summer. Walnut tee growth was 8.5 ft. for treated trees, 1.5 ft. for untreated trees.

6) Frost protection. Spraying fruit trees at full-blossom or when the blossoms begin to wither can offset the detrimental effects of frost.

7) Root formation. GA inhibits the formation of roots in cuttings.

 

[Katsu]

I've got a few tails. I really want to try to break off the outer shell on a couple of them, but I'm afraid my gorilla hands will crush the fragile beans. Batch one soaked for about 18 hours. Bach 2 soaked for about 6 days.

 

[FireIn.TheSky]

Look up seed germing machines. I have seen home made machines made from a tupperware, heat mat and a sponge. I don't rember but I think gibberish acid was used as well. I would venture out of the stoner forum and look up some real scientific techniques.

Maybe use a pair of sterilized forceps to remove the shells.

 

[Katsu]

Look up seed germing machines. I have seen home made machines made from a tupperware, heat mat and a sponge. I don't rember but I think gibberish acid was used as well. I would venture out of the stoner forum and look up some real scientific techniques.

Maybe use a pair of sterilized forceps to remove the shells.

I've ordered hemostats and gibberellic acid to mess around with. I've read mixed results on both. Most of the discussions regarding germinating old seeds are typically MJ related as other seeds are more often than not pretty readily available.

I'm starting with the least aggressive techniques first, using benign and proven techniques including heat, clean water, nutes, and hormones. I've read in a number of places that carbohydrate absorption can become an issue with older beans, and that a sugar/molasses soak can be beneficial as well.

Based on early results, I feel pretty confident I'll be able to get a reasonable percentage of these beans to pop.

 

[Morphote]

Popping Old Marijuana Seeds with GA3
[YOUTUBEIF]tehCb0Zbi1M[/YOUTUBEIF]

View Image

http://www.ebay.com/itm/Gibberellic...nt-Growth-Hormone-international-/322015376039


Effects of Gibberellic Acid



1) Overcoming dormancy. Treatment with high concentrations of GA is effective in overcoming dormancy and causing rapid germination of seed. Concentrations of about 2 ppm can cause tubers to sprout earlier.

2) Premature flowering. If a plant is sufficiently developed, premature flowering may be induced by direct application of GA to young plants. This action is not sustained and treatment may have to be repeated. Formation of male flowers is generally promoted by concentrations of 10 to 200 ppm., female flowers by concentrations of 200 to 300 ppm. Concentrations of more than 600 ppm markedly suppresses initiation of both male and female flowers.

3) Increased fruit set. When there is difficulty with fruit set because of incomplete pollination, GA may be effectively used to increase fruit set. The resulting fruit maybe partially or entirely seedless. GA has increased the total yield in greenhouse tomato crops both as a result of increased fruit set and more rapid growth of the fruit.

4) Hybridizing. Pollination within self-incompatible clones and between closely related species may some times be forced by the application of GA and cytokinin to the blooms at the time of hand pollination.

5) Increased growth. GA applied near the terminal bud of trees may increase the rate of growth by stimulating more or less constant growth during the season. In a Department of Agriculture experiment, the GA was applied as a 1% paste in a band around the terminal bud of trees. Treatment was repeated three times during the summer. Walnut tee growth was 8.5 ft. for treated trees, 1.5 ft. for untreated trees.

6) Frost protection. Spraying fruit trees at full-blossom or when the blossoms begin to wither can offset the detrimental effects of frost.

7) Root formation. GA inhibits the formation of roots in cuttings.


View Image

Directions say you have to dissolve it in alcohol first, then add it to water. Can anyone verify this, and that it works? Thanks.

M.

 

[Betterhaff]

It’s faster that way. It just takes longer to dissolve in water only. I always make a higher concentration stock solution and dilute to the appropriate concentrations to use.

 

[FireIn.TheSky]

I've ordered hemostats and gibberellic acid to mess around with. I've read mixed results on both. Most of the discussions regarding germinating old seeds are typically MJ related as other seeds are more often than not pretty readily available.

I'm starting with the least aggressive techniques first, using benign and proven techniques including heat, clean water, nutes, and hormones. I've read in a number of places that carbohydrate absorption can become an issue with older beans, and that a sugar/molasses soak can be beneficial as well.

Based on early results, I feel pretty confident I'll be able to get a reasonable percentage of these beans to pop.

I recently had seed I made in 2008 germ with 100% in 24hrs. I think a lot of it has to do with the quality of the seeds themselves.

If they were junk seeds they may not even have germed in their day.

I'm sure out of the entire lot you should get some worthwhile strains resurected.

 

[Katsu]

I would feel a lot more confident in the results if they had been refrigerated.

 

[Stash]

Katsu my friend, I still have the Bubba x's that you gave me many years ago:
Grapefruit Mango x Bubba, Oxygen 19 x Bubba, M39 x Bubba
I have grown out some in the last 2 years and all these seeds are very good. The only thing is that I make seeds and your Bubba pollen is S1 so these Bubba x's are all female seeds.
I have worked with GA3 and you don't want to use it on anything that you care about. It can kill your seeds and turn your plants into crap real fast. I'll never use it again.
Later, Stash

 

[Katsu]

Katsu my friend, I still have the Bubba x's that you gave me many years ago:
Grapefruit Mango x Bubba, Oxygen 19 x Bubba, M39 x Bubba
I have grown out some in the last 2 years and all these seeds are very good. The only thing is that I make seeds and your Bubba pollen is S1 so these Bubba x's are all female seeds.

Later, Stash

How did the crosses turn out? Anything special? I never had a chance to grow out any of them. I

 

[TnTLabs]

Katsu my friend, I still have the Bubba x's that you gave me many years ago:
Grapefruit Mango x Bubba, Oxygen 19 x Bubba, M39 x Bubba
I have grown out some in the last 2 years and all these seeds are very good. The only thing is that I make seeds and your Bubba pollen is S1 so these Bubba x's are all female seeds.
I have worked with GA3 and you don't want to use it on anything that you care about. It can kill your seeds and turn your plants into crap real fast. I'll never use it again.
Later, Stash

im with stash on this one, i would advise against using GA3, it will change hormone ratios and might induce hermaphroditism... not worth the risk!

For me its all about bacteria and enzymes, i soak them in a light kelp & enzyme mix for 24 hrs (not longer as its only to soak the shell and to get the enzyme activity going inside the shell and outside with the enzyme you added to keep the germs at bay) and then germ between a tissue in a covered saucer (id go for around 25*C) for max 2 days, checking twice a day and letting fresh air in is usefull but not a must, by then one should have cracked beans with a tap root coming through
ready to be planted in a solo cup with added bacteria and mycros

 

[Katsu]

im with stash on this one, i would advise against using GA3, it will change hormone ratios and might induce hermaphroditism... not worth the risk!

For me its all about bacteria and enzymes, i soak them in a light kelp & enzyme mix for 24 hrs (not longer as its only to soak the shell and to get the enzyme activity going inside the shell and outside with the enzyme you added to keep the germs at bay) and then germ between a tissue in a covered saucer (id go for around 25*C) for max 2 days, checking twice a day and letting fresh air in is usefull but not a must, by then one should have cracked beans with a tap root coming through
ready to be planted in a solo cup with added bacteria and mycros

Yeah. These beans are old (15+). I think I'll be lucky to get most of them to pop any sort of tail in under a week. I've read that beans this old can take 3+ weeks...

 

[TnTLabs]

Yeah. These beans are old (15+). I think I'll be lucky to get most of them to pop any sort of tail in under a week. I've read that beans this old can take 3+ weeks...

if im not mistaken, its the enzyme that wake up the embryo and start the whole process of growth, once water enters the shell.
Over the long storage time the shell has gotten really hard and this is where scuffing and soaking in a glass of water comes in handy..
Water has to enter the shell so the enzymes can begin to activate and feed the embryo. Possibly due to the long time, enzyme and food storages are hurt, so boosting with a enzyme supplement, using kelp (auxins and other growth hormones) and higher heat, you will speed up germination.
I propose you try this on a few you are not too fond of...
im positive you will see more pop than you thought