Dud Identification Collective Knowledge.

[Storm Shadow]

Good reading material to help understand everything on a more complex level

https://www.dmt-nexus.me/users/cosmicspore/The_Biotechnology_of_Cannabis_sativa_2nd_edition.pdf


Also a solid guide

http://www.ext.colostate.edu/mg/gardennotes/331.html

 

[Storm Shadow]

https://etd.ohiolink.edu/rws_etd/document/get/osu1101752677/inline


INSECT TRANSMITTED PLANT PATHOGENIC MOLLICUTES,
SPIROPLASMA KUNKELII AND
ASTER YELLOWS WITCHES' BROOM PHYTOPLASMA:
FROM STRUCTURAL GENOMICS TO FUNCTIONAL GENOMICS

The mollicutes,​

​

​

​

[FONT=TimesNewRoman,Italic][FONT=TimesNewRoman,Italic]Spiroplasma kunkelii [/FONT][/FONT]and aster yellows witches' broom (AY-WB)​

phytoplasma, are insect-transmitted plant pathogens. These mollicutes invade and​

replicate in cells of various insect organs and tissues, and inhabit and replicate in plant​

phloem tissues. They cause severe symptoms to many plant species worldwide, including​

economically important crops and ornamental plants. Their fastidious nature and lack of​

genetic tools have hampered the research on these plant pathogenic mollicutes.

 

[Storm Shadow]

http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=7642

yellow disease phytoplasmas (aster yellows phytoplasma group)

List of symptoms/signs

Fruit - abnormal patterns
Fruit - abnormal shape
Fruit - discoloration
Fruit - premature drop
Fruit - reduced size
Growing point - dieback
Growing point - discoloration
Growing point - distortion
Inflorescence - abnormal leaves (phyllody)
Inflorescence - discoloration (non-graminaceous plants)
Inflorescence - distortion (non-graminaceous plants)
Leaves - abnormal colours
Leaves - abnormal forms
Leaves - leaves rolled or folded
Leaves - yellowed or dead
Roots - hairy root
Roots - reduced root system
Roots - stubby roots
Stems - dieback
Stems - internal red necrosis
Stems - stunting or rosetting
Stems - witches broom
Whole plant - distortion; rosetting
Whole plant - dwarfing
Whole plant - early senescence
Whole plant - elongation
Whole plant - plant dead; dieback
Whole plant - uprooted or toppled


http://books.google.com/books?id=Ao1_X74EZ_oC&pg=PA102&lpg=PA102&dq=Plant-Pathogenic+Virus+witches+broom&source=bl&ots=7iQCOL0QI6&sig=oQP1H0NMPvH6ckWpf4EXTX9IrQA&hl=en&sa=X&ei=zw6OU6PBM4aIogSZrIHgCQ&ved=0CGgQ6AEwBw#v=onepage&q=Plant-Pathogenic%20Virus%20witches%20broom&f=false

http://books.google.com/books?id=ko...=Plant-Pathogenic Virus witches broom&f=false

http://books.google.com/books?id=-B...=Plant-Pathogenic Virus witches broom&f=false

 

[Storm Shadow]

Everyone should read this one

http://www.aphis.usda.gov/import_export/plants/manuals/emergency/downloads/nprg-phytoplasma.pdf

 

[Storm Shadow]

http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001835

Phytoplasma Effector SAP54 Hijacks Plant Reproduction by Degrading MADS-box Proteins and Promotes Insect Colonization in a RAD23-Dependent Manner


Abstract

Pathogens that rely upon multiple hosts to complete their life cycles often modify behavior and development of these hosts to coerce them into improving pathogen fitness. However, few studies describe mechanisms underlying host coercion. In this study, we elucidate the mechanism by which an insect-transmitted pathogen of plants alters floral development to convert flowers into vegetative tissues. We find that phytoplasma produce a novel effector protein (SAP54) that interacts with members of the MADS-domain transcription factor (MTF) family, including key regulators SEPALLATA3 and APETALA1, that occupy central positions in the regulation of floral development. SAP54 mediates degradation of MTFs by interacting with proteins of the RADIATION SENSITIVE23 (RAD23) family, eukaryotic proteins that shuttle substrates to the proteasome. Arabidopsis rad23 mutants do not show conversion of flowers into leaf-like tissues in the presence of SAP54 and during phytoplasma infection, emphasizing the importance of RAD23 to the activity of SAP54. Remarkably, plants with SAP54-induced leaf-like flowers are more attractive for colonization by phytoplasma leafhopper vectors and this colonization preference is dependent on RAD23. An effector that targets and suppresses flowering while simultaneously promoting insect herbivore colonization is unprecedented. Moreover, RAD23 proteins have, to our knowledge, no known roles in flower development, nor plant defence mechanisms against insects. Thus SAP54 generates a short circuit between two key pathways of the host to alter development, resulting in sterile plants, and promotes attractiveness of these plants to leafhopper vectors helping the obligate phytoplasmas reproduce and propagate (zombie plants).

 

[Storm Shadow]

If anyone wants to pay for this brand new article...or can get a link to it...post it up....Brand new article from May 16th 2014

http://www.nature.com/nrmicro/journal/v12/n6/full/nrmicro3283.html?message-global=remove

Bacterial pathogenicity: Phytoplasma converts plants into zombies.

 

[gaiusmarius]

will not let me buy it unless i have a US address it seems. it's only 8 bucks, hope someone gets it and posts it up, lol.

 

[dank.frank]

Making information like this accessible to the general public is what Aaron Swartz was persecuted for... R.I.P.



dank.Frank

 

[gaiusmarius]

sounds like it's exactly about what we are interested in.

 

[mofeta]

Now that article is what I've been talking about. Here is the actual research paper full text (free) that the linked article is based on. It's in the Public Library of Science (PLOS):

Phytoplasma Effector SAP54 Hijacks Plant Reproduction by Degrading MADS-box Proteins and Promotes Insect Colonization in a RAD23-Dependent Manner

 

[SaBeS]

After reading both those articles, it seems a good way to check if this is the cause it to try and pollinate a dud and see if you get seeds. Sterile plants though would go against the theory that this could pass through seeds.

 

[whatthe215]

here's a cool video on Regalia's mode of action, how it fights fungi and bacteria
http://www.youtube.com/watch?v=VP9MQpfU4e8

 

[mojave green]

Mojave green, sour sage dud from seed.
Looks like root problems if I had to guess from the photo, how do the roots look? Normal or off color and less roots?

lol, that's kind of a problem. I had to leave town unexpectedly so i shut down my system. the roots have been dry for over a week so i already fooked up my chance at any reliable root diag.

Yeah that is for sure root stress. The severe epinasty and premature leaf senescence are from a massive excess of ethylene. When roots are under stress or damaged they produce a compound called ACC, that is translocated to the rest of the plant, where it is turned into ethylene. The most common cause of this is hypoxia.

1st, thanx for the diag guys!

And i would absolutely concur with it, except that these 2 plants were in ebb and flo linked buckets full of clay pebbles, right next to each other.

the companion plant yielded 7oz's.
given the nature of my system, i can't reconcile how i starved one plant's roots of oxygen and none of the other 4 plants in the system.

It seems like I misunderstood the term "dud". I thought it described a specific phenomenon. Apparently it refers to a plant that doesn't produce right for any reason?

lol, i missed the part where there there was a consensus on what a 'dud' actually is. i thought my plant met some of the criteria. wasn't sure. if it do not i will gladly bow out and keep my hypoxiated plants to myself!

the 'dud' issue does seem to be root related though.

 

[RetroGrow]

lol, that's kind of a problem. I had to leave town unexpectedly so i shut down my system. the roots have been dry for over a week so i already fooked up my chance at any reliable root diag.

1st, thanx for the diag guys!

And i would absolutely concur with it, except that these 2 plants were in ebb and flo linked buckets full of clay pebbles, right next to each other.
View Image
the companion plant yielded 7oz's.
given the nature of my system, i can't reconcile how i starved one plant's roots of oxygen and none of the other 4 plants in the system.

lol, i missed the part where there there was a consensus on what a 'dud' actually is. i thought my plant met some of the criteria. wasn't sure. if it do not i will gladly bow out and keep my hypoxiated plants to myself!

the 'dud' issue does seem to be root related though.

What's that tripod holding the bulb? Is that a camera tripod that you adapted, or a product made specifically for that use?

 

[Tonygreen]

Asters yellow is what I posted about.
Little more to read without payin...

http://www.readcube.com/articles/10.1038/nrmicro3283

"Sap54 suppresses floral development by promoting RAD-23 dependant on degradation of plant MTFs"

 

[mofeta]

given the nature of my system, i can't reconcile how i starved one plant's roots of oxygen and none of the other 4 plants in the system.

I doesn't have to be hypoxia, other root damage can cause the same syndrome, but if i was you, I would check the inlet/outlet for a piece of hydroton that will move enough to let water in, but move back to plug the hole when the pump goes off, the pressure from the bucket full of water pushing it up against the hole, leaving the roots submerged for a sufficient amount of time each cycle to cause hypoxia.

i missed the part where there there was a consensus on what a 'dud' actually is.

Me too. That was my point, I realized that I am not sure there is one.

 

[mojave green]

I doesn't have to be hypoxia, other root damage can cause the same syndrome, but if i was you, I would check the inlet/outlet for a piece of hydroton that will move enough to let water in, but move back to plug the hole when the pump goes off, the pressure from the bucket full of water pushing it up against the hole, leaving the roots submerged for a sufficient amount of time each cycle to cause hypoxia.

once again, thanx for diag mofeta! think u r correct. tor down system tonight. roots in the fitting. will have to keep better eye on that.
can't give u no more rep for now.

 

[bobblehead]

nvm mofeta beat me to it...

 

[Storm Shadow]

For those that forgot.... the plant on the right is a DUD in veg

 

[Storm Shadow]

DUD plants will not produce Healthy Large Fan Leaves... Maybe Branches that haven't yet been effected...

This again ...DUD on the right... For those that doubt or who no idea.. here it is again ...