True enough about the lab results being the best way to tell. I've got a scope but haven't actually identified nematodes or broad mites. But according to what I've read on the UC Davis website 111 degrees for 30 minutes kills about eight to ten different nematodes and broad mites. Seems simple and non lethal to me just to be sure I don't have either. Now in all honesty I've torched a bunch of cuttings trying to maintain that 111 degree water temp. Can't walk away while doing it and it can't go above 112.5, really no higher than 112. Double boiler is best for better temp stability