What's snypes cloning method,, sounds good,,,I like over kill
What's snypes cloning method,, sounds good,,,I like over kill
That is about 14 years old. Not sure why banned.Snypes process was a good read,,,I have a few point tho
I don't think he understands tissue culture tech, ,he calls the callus that forms on the stem "root nubs",, that's not a big deal but u can tell he's not read some literature,, Anyways
I also don't think he understands the pressure principle,,,when he says that he wipes the condensation off the lids I can tell he's not understanding that one key principle is to make the pressure at the top of the prop higher than at the bottom,,that is why I suggest keeping the cubes pretty dry and the top of the cuts and the lid of the props kinda wet,,,
I love his antibiotic application,,that's a fukin genius move,,,I've considered using ppm (plant preservative mixture) ,,,but his antibiotics are very interesting
He's absolutely right about healthy mothers,,,he's absolutely right about sterilizing everything,,,
I'm working atm so can't go into more detail,,but I love his approach,,,I think he could actually go further,,,that guys a good dude tho,,,
He doesn't mention dunking,,,I think he would benefit from a hypochlorous acid dunk,,,few other things too ,,but his pretty good ,,I like the guy
Wish I had taken pictures. Greyish mold on outside of stems above coco bottom 1 foot of plant. Could be some other gray mold rotting stems. Tried taking very tops and cloning with 1 of 33 surviving. Wanted them out of house since looked hopeless and worried about health with so much mold. Had been spraying a lot to try and kill spider mites, which were immune to everything including 2 no pest strips in 5 X 5 sealed tent after 3 days and were not even phased. They were running around like on meth. Never saw them run before.Does botrytis kill mothers? I thought grey mold only took cuttings and flowers. A quick check seems to agree. Some types can cause problems with leaf spotting, but if it were taking out entire plants in veg, then it would be taking out entire plant populations.
I do now see the light.. Seeds is a russian roulette of infection rate, as most parent stock by my guesses is infected, and most seed now showing to carry infectious particles of viroid on their outside..Bleach be your best friend germinating seeds, fresh media, fresh pots, keeping drainwater away from each other for plants etc..one tool per plant or proper disinfection protocols. Clones always lose over time, as they all infected by the time we cloning them, already..it is just a slow downhill slide from there. Something that was a classic decades old, revived and clean of virii, viroid and systemic pathogenic bacteria and fungi, will completely shit on most modern stuff with varying degrees of viroid load. Though the first run from seed and maybe the second you will see amazing bud from the modern but it always goes downhill slowly from there...the pattern now in hindsight is pretty obvious..Clones are truly eternal if you just do multiplication invitro,,the only reasons they fail is they fall pray to nasties or epigenetic drift,,,most people maintain a full blown mother room and that is where the nasties get em,,,without the mother room an if you maintain invitro they are shielded,,,the only issue then is epigenetic drift and that's another topic ,,
I'd choose clones maintained invitro over seeds
They are now seeing in study on low end under 10% to high end low 40% I think transmission to seed depending on whether mom and pops are infected, and also to what degree their viral load is when the pollination occurs.Seeds are your best bet for long term growing. Clones eventually fail no matter what.
What would the real difference between peroxide and ozone in solution? completely ignorant here in that respect, would you know bro?Just wondering if anyone has used ozone water treatment??
I hear its better than hydrochloric acid and is inactive in an hour??
Cannabis does not need callous to produce roots.I don't think he understands tissue culture tech, ,he calls the callus that forms on the stem "root nubs",, that's not a big deal but u can tell he's not read some literature,, Anyways
Great info,,Cannabis does not need callous to produce roots.
Callous implies the cells are undifferentiated, but those plants are actively producing roots. It’s perfectly accurate to say those are root nubs popping straight out of the side of the stem.
Watched video and saw someone using dichlor to sanitize seeds since it weakens much faster than bleach. No idea how strong the solution was.I do now see the light.. Seeds is a russian roulette of infection rate, as most parent stock by my guesses is infected, and most seed now showing to carry infectious particles of viroid on their outside..Bleach be your best friend germinating seeds, fresh media, fresh pots, keeping drainwater away from each other for plants etc..one tool per plant or proper disinfection protocols. Clones always lose over time, as they all infected by the time we cloning them, already..it is just a slow downhill slide from there. Something that was a classic decades old, revived and clean of virii, viroid and systemic pathogenic bacteria and fungi, will completely shit on most modern stuff with varying degrees of viroid load. Though the first run from seed and maybe the second you will see amazing bud from the modern but it always goes downhill slowly from there...the pattern now in hindsight is pretty obvious..
And yes sorry guys not read the pages long discussion, just in a page back..but yes genetics can entirely be saved from fusarium/pythium/stem canker, but if it is, then most likely infected also with hlvd in my experience and then it will cost you and there is only very few doing it. And you better be testing the "clean" material for months after to make sure unless its from one of the proper places who are not BS artists or flybynites...you have to go invitro..or seed too you can as troutman says but either way it will involve lots of testing. and time and hence the cost if you outsource. But the layman only really has access to seed, though there are those who do invitro at home, its the clean up protocols that are not accessible, so seed is the only way possible for laymen. But it is not possible without lots of testing.
Good question. I'm pretty sure I read somewhere not too long ago about research that was done showing it's best to clone right after feeding the mother plant. If that's the case - why not soak the cuts in a weak nutrient solution before cloning?Can cuts from a plant that is likely infected with mold be soaked in some nutrients to give it enough strength to be healthy until roots form. Thought I read that using anything but pure water would cause problems. The tops do not look 100% probably from mold cutting off flow of water and nutrients to top of plant.
That was what I was thinking, like 300 PPM. Have 4 from one plant and might try 2 with and 2 without. Saw a product recently called lalstim, that is supposed to help cells retain water, to keep them healthier.Good question. I'm pretty sure I read somewhere not too long ago about research that was done showing it's best to clone right after feeding the mother plant. If that's the case - why not soak the cuts in a weak nutrient solution before cloning?