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Piff_cat

Well-known member
considering she was a lone survivor its very likely that
a) already had extra chromosome(s) (21 to 29)leading to larger cell organ size allowing survival. or
b) the stress led to spontaneous chromosome doubling during mitosis and became a tetraploid

there have been many instances of aneuploids in landraces but only one true tetraploid population has been found so far. it is located in darcha a cold high altitude desert biome in himalayas. this inhospitable environment is famous for creating / encouraging tetraploids due to the survival factor better water retention of larger cells etc.
the areas around darcha in himchal pradesh(shimla) there are tons of chromosome mutants of all different types. a real grab bag. landrace mafia has documented quite a few sterile females/faciated/abnormally large plants in malana/wailing farmers even point out specific areas of the mountain which are more likely to produce these outliers.
when there is a polyploid population close to a normal diploid wind pollination/seed dispersal of the tetraploid pollen will create a butterfly effect on the surrounding populations making hybrid swarms just like those seen in himchal pradesh.
pollen size and genome content is directly related to chromosome counts so its much more effective then females at passing on extra chromosomes this may be a large part of why nevils 69 males retained more of these primal traits. even without intentional selection this mixoploid pollen will persist for couple generations.
triploids meiosis(embryo creation) is the main driver of aneuploids(more then 20 but less then 30/incomplete extra set). this is due to 3 sets of chromosomes with only 2 poles to migrate towards. inevitably there are laggards,broekn chromosome arms etc.
one cool way to figure out if its an aneuploid is to self it- just like we are seeing in a5s1 a selfed aneuploid will have huge pheno variation and mutations far beyond a normal f2 spread.
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this is a crazy mango haze seedling which matches the abnomal tumor like germination in the article. the endosperm on this thing was out of control i accidentally detached it too early and the monster never recovered. dumb ass. lol
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%20Suman%20Kaushal%20Kangra.pdf
 

The Zientist

Well-known member
Veteran
It's day 45 of flowering, and 5 days since I've switched to 11/13.

A5HzS1#1​

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  • Unfortunately I had to top this one, as she was reaching the led panel. It was one of the few clones I managed to flower without topping this time, and she was displaying a beautiful upright growth.
  • She has a peculiar leaf hang, as @dank.frank commented on, and it's the least quirky, if at all, of my batch. Not much to say about this one that you can't see from the pics.
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The Zientist

Well-known member
Veteran

This is #2. Beware of the extremes.​

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  • Nightmare material for some.
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  • Her leaves only develop to have three leaflets. Sectorial and twisted variegation. She self-topped twice. Has 6 to 8 internodes on her latest.
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  • She's the queen of all freaks.
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The Zientist

Well-known member
Veteran

#4
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  • She seems to me like a bushy Colombian leaning. Gonna be a yielder, amazing structure. Thinking she could perform pretty well outdoors, despite all the variegation that's ongoing.
  • As you can see, this one experiences the photodamage I was talking about before, and she did suffer a bit of hydric stress while I was away although she bounced back quickly only losing a few bottom fan leaves and such.
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The Zientist

Well-known member
Veteran

#5

A5HzS1 mix-21.jpg

  • Another that I had to top to contain vertical growth. She was impaired by taking the same EC as all others while she was secluded in a pretty shaded corner, which led her to develop those nasty clawed leaves. She has recovered since and is stacking lil haze nuggies.
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  • Her leaves always had a distinct leathery texture and wedgy curl, not the most visually appealing and healthy looking, besides the environmentally induced claws.
 
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The Zientist

Well-known member
Veteran
That's sums it up for now. Hope you enjoyed the freakshow and my summary on them. :)

For the curious and attentive crowd, #6 is currently really hating the high output of light close to the LED panel, though I have no more room to move her around this time. I topped her but she still found a way of spearing it up. Like @Piff_cat said it was known to occur, her bottom inflorescences are developing at a superior pace in comparison with the apical. Her flower development still doesn't justify any pictures. #8 is a following the same trend, though with a better response. They both look gnarly though.


Interesting comment again @Piff_cat, about selfing aneuploids to observe how their progeny segregates.
My sample size is pretty small but the phenotypical variation among sibs, the incidence and both the quantity and diversity of these mutations/unstable variations should provide some insight about the original A5 being an aneuploid.
The dispersion is all over the place! And the rate of mutants is far beyond the scope of a normal F2, just look at my limited sample and how many are present.

It's nice to see you around @Fitzera , thanks for sharing your Golden Tiger x A5. You really did well growing her out. How did she do for you? :)
 
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Piff_cat

Well-known member

This is #2. Beware of the extremes.​

View attachment 18799445


  • Nightmare material for some.
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  • Her leaves only develop to have three leaflets. Sectorial and twisted variegation. She self-topped twice. Has 6 to 8 internodes on her latest.
View attachment 18799451
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  • She's the queen of all freaks.
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These are truly special plants the self toppers always my favorite. Also on the clawed ones this may sound crazy but it's genetic in this case. They always start off broadleaf at bottom with a thicker then normal stem as they grow internodes get very long plants stink and appear overwatered. It's very desirable karyotype been found in the doors cartel haze(a5 x cbh) Mexican death sativa . Technically they're called concave leaves. Some of the first ganja lines in Colombia came at early 1900s from borneo/aceh from the abandoned pharma grows. But the se asian/indo mixing bowl had been put in motion and soon Mexican sativas also imported for cartel production in 60s. Of course Colombia enviorment will have huge effect on the imported germplasm but in this case the cherry picked ganja lines served as base instead of normal Indian types brought most other new world locations
 

Fitzera

Well-known member
Thanks man! I just wanted to say, it's not visible in the pictures I uploaded but I have the same leaf variegation in a few spots as your #2 where the middle leaflet and the one beside it twist towards each other. I'll try to get a picture tomorrow.

She's still going right now, I've cut the lights back to 10/14 to try and get some of the more wild plants to finish in my time window. I havent noticed any maturing from her really and she hasn't begun swelling from what i can tell. Looking at her I'd say she has a few weeks left yet, seems to be growing bigger and bigger. I havent had to stake it but she's starting to sway quite a bit with the wind from the fan. Might have to before she's ready to chop.
Full on incense hazey type smell, not super pungent but you cant miss it either. Maybe a hint of citrus from the Thai side but very minimal.
Mine also must have alot of NL influence as she didn't stretch too much. Very easily manageable.

Your #5 has similar qualities to my Thai leaner in those curled leaves. What I've noticed about that on my end is it's only happening where the plant isn't getting an optimal amount of light. Ive adjusted lights and moved cobs around and that seems to alleviate the issue. Not sure if it's a similar reason as yours but I assume not.
 

Piff_cat

Well-known member
Screenshot 2022-10-17 at 12-29-59 Mexican Death Sativa repro - Overgrow.com.png



except the mexican death the rest have varying amounts of haze A and all have this weird mutant claw thing going. even if its just a sensitivity to water it still seems very genetic because they all start with broad leafs and tight nodes at bottom then flower starts internode length gets crazy. also the kartel haze (a5 x cbh) is the most mutant and has the highest haze a content. the other 3 are all males and look very similar


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Hammerhead

Disabled Farmer
ICMag Donor
Veteran
Thanks, it is the best looking plant from the lot. 3 will recover in time. these look OK. The last one is a hot mess, the worst of the bunch. Shaggy growth and very fragile. When it fully recovers ill decide its fate.

Heads up that clawing. There are 2 reasons that express as clawing..

1. Too much Nitrogen..
2. Overwatering..

The medium needs to dry well between feedings. Do not water until the pots are dry, just make sure to not wait too long or the plants will wilt. A dryer medium is always better than too wet. Soggy medium can cause root rot and other problems. A proper Wet/dry cycle has a huge impact on plant health. Feeding more water only will fix any N issues.

I would also check the ph on your runoff. Variegation can express when plants are sick. There's def some burning going on.


I feed an EC of 1.2 with a PH of 6.5 in promix hp.
 
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Piff_cat

Well-known member
ya kno i think that a modified nft/ebb and flow would be a great way to grow haze A you could even thro in the coco wicking mats underneath. i have 3 extreme nld outback viets right now in gallon pot only 2 inches of soil and they are the happiest plants in the whole tent. the leaves are 3 times bigger then anything else as is the main stem. whereever haze A originaly came from must have been a shallow horizontal type rooting system . and used to gettin indirect light as if under a forest canopy. rhododendrons for example would fit this bill and they also love acidic soil too. with ebb and flow that uses rockwool and coco capilary mats underneath would provide tons of oxygen fresh nutrient while still getting the benefits of drying root system out each time without the danger of over doing it. i can just feel the difference in a haze A plant when i pick it up. when soil is nearly dry they get the lushest.
 

Piff_cat

Well-known member
I've noticed the portion of haze A progeny that do not like direct light also tend to prefer acidic end of medium well drained lots of horizontal root action less deep roots. These viny types seem to be the leathery goodness of a5. Specifically the bh7 x Punto biches I have in veg
noticed they stand up completely straight even w fan. And then where stem meets media they have several large horizontal exposed anchor roots. I think if these get covered/stay too moist after transplant cause problems similar to over watering. My fenotrigo outbackviet female falls victim to this alot
 

The Zientist

Well-known member
Veteran
Hi everyone,

I haven't been much motivated to picture the A5 selfed progeny as they are looking far from ideal.

Nevertheless, I'll do it to document the journey and so you can also learn along my failures, while I get to know them.
I've been keeping track of how they react to changing growing parameters so I can steer their growth better in future cycles.
Turns out there a wide differential among siblings on how they react to growing conditions, pretty interesting gaps.

These measurements include EC, water temperature, leaf and air temperature, RH, VPD and PPFD. The incidence of variegation also seems to be a factor worth keeping track.

Don't get too excited, you're not getting to see any dank pictures. They are alive at least.

I'll see you all soon!
tZ
 
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Piff_cat

Well-known member
Out of my 4, 3 do not have the structure I like. These 3 are similar to your #1.. I have #8 in flower. She has the best structure. I'll flower #11 of the other 3 as these look the same. They are also very picky in their PH. #8 is the best of the bunch

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Your plants always look so happy! Best results I've had with haze A involves high temp/humidity loose soil well drained but shallow media letting feeder roots at top have full rein a mound works good. Slightly acidic root zone like a rhododendron. But the biggest single factor is keeping humidity above 60
 
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