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Male clones transformed to Female to judge male smoking quality

Sam_Skunkman

"RESIN BREEDER"
Moderator
Veteran
Well cannabis is determined by an XX/XY system, it's been tested check for some abstracts ;)
The odds of getting 6/20 instead of 10/20 is not significant. Trust me, plenty of people have bought 2 packs of seeds and only gotten 6 females. I say luck of the draw, you want to redefine your conception of cannabis sex from this?

The environment has a significant effect on sex ratios in Cannabis spp. This is not luck of the draw as much as one might think.

I don't see it. I don't understand it in Sam's OH but he was running big numbers not making his judgments off a sample of 20.

The 6/20 number is a reference to the sex ratio that I observed after several applications of BAP. This ratio contradicts the understanding that BAP induces higher then normal female/male ratios.

My observation for an all male population was 6/6 plants being male. The odds of observing this in a pure XY inheritance pattern is ~.5^6 or ~1.6%. I figure I had odds of .5-3% of finding 6 males of 6 plants from "random" bag seed of a single cross. My real odds were probably closer to .5% because, if I recall correctly, I grew another unrelated cross along with the all male population and got over 50% female. The environment was an unlikely factor in the development of 6/6 males.

I think that Cannabis spp. is better represented by an x1x1,x2x2 xx/xX denotation or something like that. I am not disputing a sex chrosome that caries a male gene that is not subject to recombination exists. I am disputing how we reference it. The Cannabis sex chromosome is actually bigger then the rest of the 19, not smaller. Hence, it cannot be considered a Y unless Y means male chromosome that can be influenced by recombinant genes. I think xx/xX is more befitting because the male chromosome in Cannabis spp. is larger then the rest, not smaller. Also the x1,x2, etc... would reference modifier genes in the autosomes that do exist in the world wide gene pool.

Anyways, I still think that it would be adventageous if one were to apply some BAP and Auxins in combination with the Ethephon.

Also, one might just get some fertile seed off of their male and grow those out. The resulting selfed seed should give a fair sample of the likely recombinants and the reletive homogenosity of its genome.

I think you should try the tests with Ethephon + BAP/Auxins if you think they will be interesting. Will they be safe to consume? Ethephon is.
FYI, I have gotten all female or all male many times if I grow 10 seeds or less, if I grow lots more of the same batch of seeds the sex ration is 50/50. Its like flipping a coin, do it enough and you will get 6 heads or tales in a row.
I have selfed females many times and can say there are zero homogeneous Cannabis varieties, all are heterogeneous, regardless if wild or cultivated, hemp or drug. A homogeneous variety can be man made by selfing to S5 or S6 generation, but the plants will have zero vigor. I would suggest selfing a female not a male as males are harder to self and work with as well as 25% of the seed will be YY which may or may-not grow. I confirmed my experimental selfed S5's were homogeneous by sending the materials to a lab for testing.
I did just self my male turned female and the seeds are setting.
Can you list or give examples of any "modifier genes in the autosomes that do exist in the world wide Cannabis gene pool"?

Oh, and BTW, the male transformed to female does have typical Skunk #1 terpene smells, but like I said the bracts are much smaller, maybe because of the transitory effects of Ethephon, next time I will spray 4 times 1 week apart, not three, maybe it will help with the female bract size. Also I will not spray the leaves as it killed them, that did not help.

-SamS
 
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elmanito

Interesting thread.I had a male Danish Gold strain with some female flowers.Never seen this before.I used this strain to pollinate my Lebanese strain.Plenty of seeds are harvested, so we will see what will happen with the next generation.

picture.php


Namaste :smoweed: :canabis:


 

Sam_Skunkman

"RESIN BREEDER"
Moderator
Veteran
What you have done is to breed with an inter-sexed male. This will pass on inter-sexed genes to the progeny.
-SamS
 
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elmanito

What you have done is to breed with an inter-sexed male. This will pass on inter-sexed genes to the progeny.
-SamS

So this means i can expect a monoecious line of the next generation, like what is done in the modern hemp industry today.I assume you can breed it out again.

Namaste :smoweed: :canabis:

 

Mr. Stinky

Member
elmanito, please post what actually happens with your seeds. theres so much conflicting information on this site, its hard to decide whats true and whats false :)
 
E

elmanito

elmanito, please post what actually happens with your seeds. theres so much conflicting informations on this site, its hard to decide whats true and whats false :)

I will do that.Most of the time there is a difference between theory and practice.;)

Namaste :smoweed: :canabis:

 

GreenintheThumb

fuck the ticket, bought the ride
Veteran
Hi, Sam
Appreciate what you're doing here. Think you could get us a full shot of the he/she? I'd love to see some of the damage caused to the leaves and it'd be nice to have a shot of the structure of the male pods to compare to the structure of the nice lady parts. Thanks a bunch.
 

Kanye WeED

Active member
WOW, just read the first 5-10 posts or so and i am amazed by how science or chemistry which ever of the 2 can be manipulated by us soo very much and its soo cool to do, and id so like to try this stuff but i was wondering would it be worth it on a small scale grow?

and can u pic this stuff u guys talk about up at a WALMART or lowes or something

ETS i think it was and what ever turns men into women and the women into men

thats kinda funny to say and really unnatural sounding

i would dare not smoke anyhting i have chemically altered, but im wondering once tanted for traits that one likes, wont the pollen and thus the off spring be tainted as well or will it be pollution free from seed?

thanks !!!
 

GreenintheThumb

fuck the ticket, bought the ride
Veteran
Have the seed set? Been reading all of Charles old posts and thought this could fit here.

"Whether 'male' plants can produce what appear to be female flowers is not in question; that's been documented. True 'male' plants will not produce pistillate flowers unless chemically/hormonally manipulated. Furthermore, these plants cannot produce seed, because two 'X' chromosomes are needed in order to produce ovules. The predominantly staminate flower producing plants (with late pistillate flowers) that Clarke refers to, are examples of an extreme expression of the intersex trait and as such, are not true 'males'.

Sincerely,
Charles."

I guess he just meant true males can't naturally self or cross to another male because Chimera made s1s of Dj's blueberry dad.
 

*mr.mike*

Member
Cannabis certainly isn't x/y sex determined. Sure, there are markers for male plants, but not as sex chromosomes, like in people, or even other plants.

Males have the markers for maleness, and females don't have them.

"Hermies" DON'T have any male-related markers.

Here are some funny pics of male plants induced to be female, and the transitory effect of ethylene.

I don'y know if you can tell by the pics, but the female flowers that did form really didn't get any trichomes, but did grow lots of little cystolith-type hairs.

When the treatment was stopped, the male flowers grew out of the female flower bracts.

I was going to post my "clone from a root" picture, but I couldn't find it.
 

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Sam_Skunkman

"RESIN BREEDER"
Moderator
Veteran
I did finish the plants.
They are very resinous and very strong smelling, sweet by the way, not surprising.
I will try smoking them soon, but as a non-pot smoker, I am only doing for science.
They are almost dry and did set some seed that I made by turning the top of the male clone to female and using the pollen from the bottom male flowers to pollinate the "female" flowers. The seeds should be 25% YY super male if I am lucky. If I breed with these to a normal XX female all the seed produced should be XY, male.
Then I guess I need to transform all the males to females to get a smoke???
Just joking....
-SamS
 

GreenintheThumb

fuck the ticket, bought the ride
Veteran
How do you plan to authenticate the YYs, says the guy jealous to your access to lab equipment. :D Thanks for the update.
 

Sam_Skunkman

"RESIN BREEDER"
Moderator
Veteran
So I did smoke a few bongs of the female flowers from the transformed male, sweet, great taste and smell, great potency, typical Skunk #1. I also ground up the rest and made one bong of resin, total FMCD, but not quite as good as my best female Skunk#1 resin.
This method can be used both for judging what smells and tastes are passed on by males as well as the type of high and strength.
-SamS
 

GreenintheThumb

fuck the ticket, bought the ride
Veteran
:D cool beans, thanks for the updates.

Now about your plans with the potential YYs :) The lab'll just tell you how many Y chromosomes? Can you tell me how many viable (looking) seeds you were able to harvest? Pretty cool stuff IMO.
 
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