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Tutorial DIY STS Mixing/Using Guide for Feminized Pollen/Seeds R.C.Clarke Method as Base

TychoMonolyth

Boreal Curing
50 ml sts and 450 water would still be 1:9 right?
>>With 50ml STS, you do mean with that a combination of 25ml Base A and 25ml Base B mixed together?
If so, then yes, you just add then 450ml of distilled water.
(Also do not mix the remainder of you Base solution together. Keep them separated until you make more final solution.)

.5g of Silver Nitrate is indeed very very small. So don't worry if you think that you might have missed there.

You bought 10g of Silver Nitrate seeing on your picture That's enough to make 200 liters of final solution. You can experiment for a while. (Price/weight I would have probably bought the same, I'm just merely pointing out that you got lots).

Here a snippet of my personal notes that you might find interesting. I'll give some more information about it.


As you can see here, I germinated a seed and when it sprouted I planted it into soil.
I then started taken notes the day the seedling popped the surface of the soil. This was on date 10 April.

At date 24 April I switched the timers to 12/12, meaning it was 14 days old when I switched to flowering. It was still very small.
I didn't started spraying because it only had a few internodes and no side branching.

10 days after switching to 12/12 I sprayed a first time.
I then sprayed an additional 2 times (IIRC).

As for result see the previous picture I posted for you.
lmao...

That's why I cut my recipe down. I had a quart of the stuff hanging around for a year (still viable). Now I mix a very small batch to use and dump it when I see pollen sacs.
 

Tonygreen

Well-known member
ICMag Donor
Veteran
Since I have a separate plant to reverse and host seed I'm thinking I'll put the big mom in 7 days after.
My GB bx5 finishes in 9 weeks.
I think if I stop spraying around 14-17 days maybe pollen will drop by day 28 or 30 then that big flower growth around 35 to 42 days would be the time for major pollen like yours. The mom would be 28 to 35 days over that time.
That would be spray on day 7,12 and 17.
Probably over thinking it but it doesn't seem like I should be spraying past 21 you think on this 9 weeker? In other words stop around first balls and let flowers form not keep spraying until there are giant clusters.
 
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Cvh

Well-known member
Supermod
Free ☕ 🦫
I find it hard to say. I don't know your plant, I don't know your specific situation and I don't know that I fully understand you about when you plan on start spraying, how old and big it is and the frequency. (FYI, I'm non-english).

Also take in mind that pollen sacs need several weeks too to develop and mature.
A plant that flowers 9 weeks can easily go an additional month or more when you are letting seeds mature and you're not growing for seedless. It can continue on growing for quite a while beyond the typical seedless harvest point before dying.

As a tip, why don't you practice different spraying regimes on the same plant? Treat one branch in a certain way and treat another branch in a different way. Compare the results.

Maybe if you like you could document your experiment here. Do a little log. It might help future people too even if it's not a successful experiment in the end.
 

CannaRed

Cannabinerd
I don't have the data.

Also it's recommended that you should add a surfactant at this stage. It greatly helps with keeping the final solution physically on the plant material and having it better absorbed that way.

I use Yucca extract as what's recommended by the Op.

The only yucca I have is in a bottle of Sledgehammer.
I do have Optic Foliar Transport, and have heard of folks using it for this purpose.
 

TychoMonolyth

Boreal Curing
I don't know if anyone mentioned this so...

After a few years making seed, I have a pretty good way to clean my seeds, but there's always room for improvement right?

Once you collect your seeds, you still have to sort the mature from immature. That's to say, get rid of the pale immature seeds. It's not a big issue for only a few hundred seeds, but with thousands, it's yet another task this lazy stoner doesn't like to do.

If possible, grow your mother in one room. She should self seed and you will get a mix of mature and immature seeds.
picture.php


If you want to pollinate another girl, move it to the room/tent at peak pollen production for a couple days. Give the mom a good shake, then move the target plant out to make sure she doesn't get any more pollen as more white hairs appear. This way most seeds on the target plant will mature at the same rate and will reduce pale immature seed production. Of course, you want a lot of white hairs when you do this.



As for pollinating in the wild, I wonder if I can "spray" pollen so I can walk in, give the plant a couple shots, and walk out. Screw this paint brush shit. :D Anyone know if adding pollen to water in a hand sprayer would keep pollen viable?
picture.php
 

Cvh

Well-known member
Supermod
Free ☕ 🦫
Don't mix pollen with water. It will make the pollen sprout and render it useless.
Check out this thread, I have posted several pictures and a video about water and pollen for the exact same question if this could be done for outdoor pollination.
https://www.icmag.com/ic/showthread.php?p=8920907

To 'spray' pollen. Have you ever thought about using a powder blower? It's used in the garden to disperse powders also barbers use small ones to disperse talc powder. I haven't bought one because I'm a cheapskate. But I think it might work. Powder is powder no?

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TychoMonolyth

Boreal Curing
Don't mix pollen with water. It will make the pollen sprout and render it useless.
Check out this thread, I have posted several pictures and a video about water and pollen for the exact same question if this could be done for outdoor pollination.
https://www.icmag.com/ic/showthread.php?p=8920907

To 'spray' pollen. Have you ever thought about using a powder blower? It's used in the garden to disperse powders also barbers use small ones to disperse talc powder. I haven't bought one because I'm a cheapskate. But I think it might work. Powder is powder no?

View Image
View Image



Ah... I'll try it next season.

I love that powder blower. I'll look for one.

Add pollen, shake shake, check wind, puff puff. :headbange
0302411_1_S.jpg



EDIT: OK.. scratch my vinegar bottle idea. There are LOTS, in all sizes, on Amazon.
DV-119_Powder_Blower_cat_large.png
 

Cvh

Well-known member
Supermod
Free ☕ 🦫
Yh, maybe a little one like that may be better. Or you should have hands full of pollen for a bigger one.
 

TychoMonolyth

Boreal Curing
Yes. I cut it 4 to one. So I have lots.

But you really only need a bit with this. It's not like you're dusting for earwigs. lol
 

TychoMonolyth

Boreal Curing
So tyco, if I got it right your go to is 1:6 2 sprays 14 days apart?

Yes, but as you've seen in here, once a week for 3 weeks works as well.

When I go to spray the 3rd time I usually see pollen sacs starting. I definitely stop once I do see them forming.
 

Freakazoid44

Active member
Giving up on the first plant

Giving up on the first plant

Keep picking pods and chrcking for pollen content over the next several weeks...u may get some yet.


Not much change in the plant. Collected a bigger haul of the flower pods, not seeing any pollen. I figured I'd run this batch through the strainer, dust a couple flowers, move this plant to a different closet and hope for the best.

Things looked pretty much the same after rubbing it through the strainer..... except my finger.....



Look like we got a little pollen after all. There are still some more flower pods on there, so I'll collect some more next week and save that pollen for another time. The female flowers on that plant already started turning brown, because they're at the normal harvest time for that strain. I dusted a few small buds, harvested the rest, and if it looks like some seeds develop I'll keep that plant going for another month or two. I'll save the rest of my flour-diluted pollen from this batch and the remaining pods on the plant and I'll go for seeds next time I grow that variety.

Semi-successful, in spite of myself. Thanks to everyone for the help.

My C99 gets its 5-day pre-spray tomorrow. I expect much more reliable results for that plant.
 
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G

GYOweed

For us small timers who suck at cloning and dont have time n space what about a 75 Micron Nylon Mesh bag?
MMJ Pollen is 25-30 microns but we're not pushing anything through and u can double it anyway.

Then when you see balls the branch covered should still see light and get air.

You can stick a paint brush in there if you wanna make S1s or just leave it and cut the whole thing when it's ready. No more needing to clone or pollinate more than you want.

https://www.amazon.com/gp/product/B075DCGDWZ/ref=ox_sc_act_title_1?smid=AK0YZD7QTKE87&psc=1
thoughts?
 

gorilla ganja

Well-known member
I really think it's that day 14 spray when sex organs begin to really start that counts most. I cant see why 2 weeks before flower would help in my mind. He's got one looking just like cvh tho and what do I know. Lol


I think the early applications are important for a quick reversal. I work with Autoflowers a lot and start applying STS when they first show sex.
The autos show and then they are full-blown into flower so they develop quite a few female flowers before I can see any male pods show through. In my mind, this slows the reversal down much the same as taking clones when in flower. Adds a week or so if they are flowering female before they can develop the male sex organs.

By applying STS before flowering is initiated ethylene production is stopped early in the flower cycle less female flowers form and less confusion for the plant.

Just a theory based on what I have seen from reversing a few times.

Peace GG
 

Tonygreen

Well-known member
ICMag Donor
Veteran
Edit* nevermind, forum recipe seems totally diff than sigma. Trying to find the mM of forum strength.
Doesn't appear to be 1:4?
.5 silver to 500 ml
2.5 sodium thio anhydrous to 500 ml

Sodium stock appears to be roughly .0316M and Silver stock .0058M?
Is my math off anyone?

So is the 100 ml stock sts forum solution is what concentration?
Is it .0374 M ???


Silver nitrate 169.87 molar mass
Sodium thio anhydrous 158.11

Trying to figure it out so I can compare mM of the forum and all of the anecdotal stories of dilution rates and see where they fall compared to.3mM to 3mM hemp reversal study.
 
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LostTribe

Well-known member
Premium user
I am at 67 days tomorrow but not sure when they got pollenated. Guess I will try to open a few pods and check for maturity. What do you guys do? I normally wait 2 extra weeks than normal. Getting close.

Thanks
LT
 

brickweeder

Well-known member
Edit* nevermind, forum recipe seems totally diff than sigma. Trying to find the mM of forum strength.
Doesn't appear to be 1:4?
.5 silver to 500 ml
2.5 sodium thio anhydrous to 500 ml

Sodium stock appears to be roughly .0316M and Silver stock .0058M?
Is my math off anyone?

So is the 100 ml stock sts forum solution is what concentration?
Is it .0374 M ???


Silver nitrate 169.87 molar mass
Sodium thio anhydrous 158.11

Trying to figure it out so I can compare mM of the forum and all of the anecdotal stories of dilution rates and see where they fall compared to.3mM to 3mM hemp reversal study.

I prefer the sigma formula rather than the robt clarke/curtis formula because it makes a nice even .02M STS solution, which is very easy to dilute to your desired concentration. It's much simpler to get to a .01M or .005M solution starting from .02M than starting from .0374 M or whatever the clarke formula calcs out to be.

I think sigma uses the 4:1 silver to thiosulfate because silver thiosulfate is at max equilibrium at that ratio, but 5:1 works well too.
 
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Tonygreen

Well-known member
ICMag Donor
Veteran
I did notice a reaction while making sigma stock sts. Slight brownish tinge that seemed to go away as I began stirring faster. And staining from drops.
Didnt get that mixing Clark's or anecdotally the "forum" recipe.
Both resulted in a clear solution with a slight blueish tinge if it catches the light right.
 

LostTribe

Well-known member
Premium user
I did notice a reaction while making sigma stock sts. Slight brownish tinge that seemed to go away as I began stirring faster. And staining from drops.
Didnt get that mixing Clark's or anecdotally the "forum" recipe.
Both resulted in a clear solution with a slight blueish tinge if it catches the light right.

My 1:9 turned brownish this time it was outside the tent in the mostly dark. Last time not sure. The stock solutions are both still clear and there is a light leak in that storage.
 
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