Tutorial DIY STS Mixing/Using Guide for Feminized Pollen/Seeds R.C.Clarke Method as Base

[LostTribe]

More pollen started dropping but not much. I do se plenty of young seed growing. So I removed the lowers that had been reversed, dried them in a dehydrator to get them a bit drier (probably will forgo that next time) then ran it through a ninja food processor just enough to break it all up good. Then I put it in an old 220u bubble bag and shook it over a table. As you can see from the color there is still some plant matter left behind but the cup from the ninja was heavily coated in yellow powder so I know there is plenty of pollen in there also have had a terrible headache and some sinus issues since I collected. Will test it out with a brush on a fresh cut of C99#1 that is 3-4 weeks into bloom tomorrow.

Thanks for everyone contributing to this thread. I'd also like to say that the spray bottle I had the STS in has turned dark this time was left outside the tent which is mostly dark, while the processor fluids have remained clear.

LT

 

[Freakazoid44]

Nervous again

Nervous again

Looks like quite a few flowers have opened, and few pods dropped, but I'm not seeing any kind of a dusting, at all.

I went through and tried to collect the opened flowers or ones that opened to the touch and fell off.

What do you think - empty flower pods, or do I stand a good chance of getting pollen from opened pods?

I have them in a jar with dried raw rice and in the fridge. Should I let them dry out a bit (if so, how long) before putting them against a screen, or should I try that, immediately?

Here's a photo of what they look like.....

 

[LostTribe]

Looks like quite a few flowers have opened, and few pods dropped, but I'm not seeing any kind of a dusting, at all.

I went through and tried to collect the opened flowers or ones that opened to the touch and fell off.

What do you think - empty flower pods, or do I stand a good chance of getting pollen from opened pods?

I have them in a jar with dried raw rice and in the fridge. Should I let them dry out a bit (if so, how long) before putting them against a screen, or should I try that, immediately?

Here's a photo of what they look like.....[URL=https://www.icmag.com/ic/picture.php?albumid=84345&pictureid=2088457&thumb=1]View Image[/url]

Plants take time to show they've been successfully pollenated. My recent reversal even with just 2 sprays total took longer than expected to drop pollen. The plants now show plenty of young seed starting to grow. More than I would have expected. I can always reverse another cut so decided it was time ~6 weeks to try a different approach and dry, grind and sieve. Tried brushing some yesterday and already can confirm a few hairs have gone bright orange and its still very early on with that fresh cutting at 3.5 weeks in.

 

[Freakazoid44]

Plants take time to show they've been successfully pollenated. My recent reversal even with just 2 sprays total took longer than expected to drop pollen. The plants now show plenty of young seed starting to grow. More than I would have expected. I can always reverse another cut so decided it was time ~6 weeks to try a different approach and dry, grind and sieve. Tried brushing some yesterday and already can confirm a few hairs have gone bright orange and its still very early on with that fresh cutting at 3.5 weeks in.

I'm not talking about pollinated, I'm talking about pollen. I can't try to pollinate anything if I don't have any pollen. I'm talking about my plants, and the flowers in the picture I posted, not yours.

I'm not seeing any pollen in those male flowers that opened and/or fell off. There's no dusting on surrounding leaves.

If it's just not dropping out, but it's there, do I need to dry out the pods before rubbing them through a mesh screen? If I need to dry out those pods, how long are we talking about if it's in a closed jar with raw dried rice?

 

[brickweeder]

Freak, if the fresh pods are full yellow and very soft and puffy, then you can just sieve them and use the pollen fresh. I've done this and its very effective. When I dry the pods, I let them sit in a cup cake paper thing out in the open (not sealed in a container with rice), for 1-2 days, then sieve. Hope you get some viable pollen soon.

Based on your pic, I'd take half of those pods and sieve them now to see what they yield....just need a small amount.


Another way is to examine the pods closely with a magnifying glass, and for any that are open or on the verge, take tweezers to the pods and and brush them up against your bud of choice (the pods work just as well or better than a paint brush for dusting your fems and are less likely to break stigmas).

 

[Freakazoid44]

Freak, if the fresh pods are full yellow and very soft and puffy, then you can just sieve them and use the pollen fresh. I've done this and its very effective. When I dry the pods, I let them sit in a cup cake paper thing out in the open (not sealed in a container with rice), for 1-2 days, then sieve. Hope you get some viable pollen soon.

Based on your pic, I'd take half of those pods and sieve them now to see what they yield....just need a small amount.


Another way is to examine the pods closely with a magnifying glass, and for any that are open or on the verge, take tweezers to the pods and and brush them up against your bud of choice (the pods work just as well or better than a paint brush for dusting your fems and are less likely to break stigmas).

Cool. Thanks for the advice. If I seem like I'm flailing, it's because I'm a newb at this.

 

[Freakazoid44]

Meh. I'm pessimistic. Rubbed it through a mesh screen (I need to get one with a finer screen), looked at it under a microscope, and it looks like it's all plant matter. Not seeing anything that looks like pollen grains or dust. I think the fact that I kept spraying it, out of ignorance, for almost three weeks, every five days after the switch to flowering lighting might have rendered this one impotent. From what I'm reading, that's twice as many spray applications as is needed, or even more.

Still, I mixed it with a bit of flower and dusted one of the non-sprayed buds on the plant. We'll see. If this one's a failure, not the end of the world. I'll post back in a couple days and let you know how the dusted flower bud is looking. I have a couple more seeds of this variety, so I can still get pollen/seeds in the future.

I have a C99 that's almost ready to flip, and I have two more of those that will stay, a couple weeks behind, so I will apply the lessons learned. I'm going to spray 5 days before, day of switch, 5 days after and that's the max.

 

[brickweeder]

Keep picking pods and chrcking for pollen content over the next several weeks...u may get some yet.

 

[Freakazoid44]

Keep picking pods and chrcking for pollen content over the next several weeks...u may get some yet.

Sounds like a plan.

 

[LostTribe]

Meh. I'm pessimistic. Rubbed it through a mesh screen (I need to get one with a finer screen), looked at it under a microscope, and it looks like it's all plant matter. Not seeing anything that looks like pollen grains or dust. I think the fact that I kept spraying it, out of ignorance, for almost three weeks, every five days after the switch to flowering lighting might have rendered this one impotent. From what I'm reading, that's twice as many spray applications as is needed, or even more.

Still, I mixed it with a bit of flower and dusted one of the non-sprayed buds on the plant. We'll see. If this one's a failure, not the end of the world. I'll post back in a couple days and let you know how the dusted flower bud is looking. I have a couple more seeds of this variety, so I can still get pollen/seeds in the future.

I have a C99 that's almost ready to flip, and I have two more of those that will stay, a couple weeks behind, so I will apply the lessons learned. I'm going to spray 5 days before, day of switch, 5 days after and that's the max.

You have 2 choices son. Wait and hope or wait and cut off the pollen sacks. Dry them, grind them, sieve them. Who's Cinderella anyway? Mine is the Original.

This is not rocket science by any means! HAHAHA!

Cheers!

 

[LostTribe]

After 48 hours I am seeing bright orange hairs on all lower pistils I brushed and a few on the tops. This is a cutting that has only been pushing pistols for ~2 weeks and only 1 month of 12/12. Strain finishes in 52-58 days.

Think outside the box or you will always be a slave to it.
LT

 

[CannaRed]

I want to try this on my PurpAl cut. I ordered the silver and such long ago.
I just received a 2x2x4 tent that I set up this week to quarantine her.

Now I need to figure out which recipe to follow.

Are certain methods better for plants that lean more indica or sativa?

 

[Cvh]

To all who is having empty pollen sacks, pollen sacks not opening or infertile pollen.
Can you confirm that you followed the exact procedure that's written in the Op post? Or did you spray more then was written down?

The current idea is that overspraying might lead to empty pollensacks and/or infertile pollen. It's been stated several times here in the thread.

Also me and several others have also stated that the current spraying regime that is written down is already very strong. A bit less might possibly even be better.

Could all who is having troubles confirm they sprayed more or that you followed the exact procedure? This is very interesting information to data collect and might help other growers.

 

[TychoMonolyth]

To all who is having empty pollen sacks, pollen sacks not opening or infertile pollen.
Can you confirm that you followed the exact procedure that's written in the Op post? Or did you spray more then was written down?

The current idea is that overspraying might lead to empty pollensacks and/or infertile pollen. It's been stated several times here in the thread.

Also me and several others have also stated that the current spraying regime that is written down is already very strong. A bit less might possibly even be better.

Could all who is having troubles confirm they sprayed more or that you followed the exact procedure? This is very interesting information to data collect and might help other growers.

The first time I did this 3 years ago, i not only sprayed every couple days, but with a very strong mixture. I got enough pollen sacs to fill a jug, but zero pollen.

The next year I used a 1:5 ratio, sprayed 5 to 10 times, and still got empty pollen sacs.

My behavior is a true addiction behavior. MORE IS BETTER. Clearly, recovering addicts and AA members know that's definitely not true.

Last year I threw caution to the wind and made a 1:6 ratio, sprayed once, waited 2 weeks and sprayed again. I waited another two weeks and was going to spray again, but only inspected while resisting the intense urge to give it another little spritz.

Eureka!

This is this year's haul from only a few branches.

30k Honduran female seeds

All I can say is "Read the fucking manual." Lol

 

[Freakazoid44]

You have 2 choices son. Wait and hope or wait and cut off the pollen sacks. Dry them, grind them, sieve them. Who's Cinderella anyway? Mine is the Original.

This is not rocket science by any means! HAHAHA!

Cheers!

I actually had the original going for a while - I was able to pop some 12 year old seeds a while back, but then I killed my clones by, you got it, being impatient. I added a touch of rooting hormone solution to my water cloner setup to see if that would get some stubborn cuts to shoot roots faster, and the clones were all rotten and dead within a week.

The one I'm currently prepping is from Female Seeds. Not sure if you have any insight into how "legit" or like the original it is.

I'm thinking in April or May, I'm going to start a grow with multiple Original Haze plants (if the 15 year old seeds pop), where I spray the some lower branches on all the plants, and then pull any authentically, originally males when they show sex (maybe wait until they are relatively mature so I can keep flowered cuttings and harvest ordinary, non-feminized pollen in case I can't get any feminized), and see if I can harvest some Original Haze feminized pollen and/or seeds. Also, never smoked Original Haze, so I wouldn't mind trying some buds from the other end of the plant.

 

[Freakazoid44]

To all who is having empty pollen sacks, pollen sacks not opening or infertile pollen.
Can you confirm that you followed the exact procedure that's written in the Op post? Or did you spray more then was written down?

The current idea is that overspraying might lead to empty pollensacks and/or infertile pollen. It's been stated several times here in the thread.

Also me and several others have also stated that the current spraying regime that is written down is already very strong. A bit less might possibly even be better.

Could all who is having troubles confirm they sprayed more or that you followed the exact procedure? This is very interesting information to data collect and might help other growers.

Yes and no on the OP post. I didn't see anything on there that indicated when one should STOP spraying. So I used the correct ratios, but kept spraying away.

Spray every 5 days until major male flower formation has begun

I took "major" to mean the flowers were getting bigger, not just generally widespread.

So in my case, I sprayed an additional 3 or 4 times, over the course of an additional 2 1/2 weeks beyond where it now seems I should have stopped.

My difficulties with this, my first plant that I've tried this on, definitely seem to be from too much spraying.

 

[CannaRed]

The first time I did this 3 years ago, i not only sprayed every couple days, but with a very strong mixture. I got enough pollen sacs to fill a jug, but zero pollen.

The next year I used a 1:5 ratio, sprayed 5 to 10 times, and still got empty pollen sacs.

My behavior is a true addiction behavior. MORE IS BETTER. Clearly, recovering addicts and AA members know that's definitely not true.

Last year I threw caution to the wind and made a 1:6 ratio, sprayed once, waited 2 weeks and sprayed again. I waited another two weeks and was going to spray again, but only inspected while resisting the intense urge to give it another little spritz.

Eureka!

This is this year's haul from only a few branches.
View Image

30k Honduran female seeds
View Image


All I can say is "Read the fucking manual." Lol

Tycho, to get to the 1:6 ratio that works for you, you used 50ml of each stock solution and added that to 600ml of distilled/to?

 

[Tonygreen]

Has anyone tried this guys spray schedule?
https://twenty20mendocino.com/sts

Spray every 3rd day until pollen drops.
Think it would be a weaker formula to spray so often?

Getting ready to take an attempt. I'll post results.
Entirely unsure what recipe I'm going to use but I'm about ready to give it a shot.

 

[Tonygreen]

I started as soon as I saw the first hairs. Maybe I should have sprayed sooner with a softer concentration. The plant gave me tons of pollen, but I really only wanted one branch to blow up. Not the whole plant. I will get a few seed out of it because a few buds weren't affected. I hope there are a few seeds under all those pollen sacs.

LSD-25 Autos.

View Image
View Image
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Was this the 1:6 ratio?

 

[LostTribe]

I used DC's 1:9 ratio from page 1. Sprayed 5 days prior to flip, day of flip and day 5 only. I had sacks open just not as much as the last time. This plant blooms in 50 days so that might have been some of the issues and I would have waited but I can always do another cut if this dried pollen doesnt work well.

However, I already have brushed the ground up sacks and haven a decent amount of pistils prematurely going bright orange in only 72 hours.

The other half of the plant, the upper, was not sprayed and was unaffected just the same as with my other c99 mom.

Just takes more time for them to open. I have read that when they look like this that they have a hard time opening. I will not spray 3 times ever again. 1 time 5 days before flip will get the job done.

Never had true males that were so ballsy!