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Dankwolfs rks breeding project and breeding info discussion

MindEater

Member
Unfortunately it seems either very hard to positively test for thiols in cannabis or something else odd is at play. I fully agree with the concept of thiols but have only seen one test that showed a thiol possibly from cannabis.

My guess is that it may be a thioester/thioflavanoid/alkanethiol etc that may be coming up as other things in tests. There has never been a definitive study that showed thiols or any other constituent as being responsible for the "skunky" smell in cannabis that I am aware of, and if you were to find such a thing showing it to me would make me a very happy person.

Whatever it is that causes it, it seems to be very elusive, sometimes evaporating before people can accurately test it say some extractors. We do know that cannabis loses its skunky smell and flavour more quickly then other strong flavour/smell elements present in cannabis.

There is something odd at play. It's the fact that Cannabis labs only test for terpenes,which have very little to do with natural Cannabis. And the fact that Cannabis breeders don't consult science specialists.

"It is not obvious how the mono- and sesquiterpenes in cannabis can be responsible for the skunk, diesel, and earthy notes observed in our samples. These characteristic notes may result from synergistic effects among terpenes or from the overlooked presence of non-terpene compounds" Dr Avery N. Gilbert

Gee I wonder which possibility it is, since Cannabis labs only test for 16 terpenes prominent in other industries..? It's the same problem as the Thc myth. The most abundant molecules by volume must be the most potent, most characteristic, right? A perfect example of flawed science.

Every other plant containing thiols has its constituents quantified via HD, GC-O AEDA and SAFE analysis, why doesn't Cannabis? We need to know what thiols are in Sesame seeds, but not Cannabis? I'm not sure why the entire scene took this terpene nonsense and ran with it like they did, other than the obvious (the perfumed distillate market). What are the most coveted strains? Chem Dawg, Bubba Kush, Sour Diesel, Skunk #1, no terpenes in those.

The fact is, skunk is not delicate or elusive. A pure skunk will get louder over time, as it's mechanism has nothing to do with terpene biosynthesis, but is the result of enzymes breaking down fatty acids. RKS had multiple genes for the right fatty acid and multiple genes for the right enzyme. I'm sure those marker assisted operations are all over this. For the backyard breeder you'd have better luck avoiding anything with terpenes. Run a thousand seeds and cull anything that smells like a mango when rubbed. That's only a week of work and not much space.

It's not trivial, to anyone rolling their eyes. RKS is not a novelty. It has a use and a purpose. This terpene nonsense is literally holding back research, holding back cancer cures, and medicinal dispensaries are making millions selling useless placebo crap to sick patients by the ton. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6749504/

" Two recent studies challenged the concept of the inter-entourage effect and concluded against its reliability. Blasco-Benito et al. added a cocktail of several terpenoids, including ?-pinene, linalool, ?-caryophyllene, ?-humulene, and nerolidol to THC at the same concentrations present in the inflorescence extract but did not record any enhancement in cytotoxic activity over that of THC by itself. It was suggested by the authors that other compounds (or compound combinations) present in the crude extract of cannabis are responsible for the superior potency over purified THC "

If you ask me, the commodization/normalization of Cannabis is anything but beneficial to humans, only to corporate drug dealers. Seems there's an agenda to make Cannabis a medicinally, therapeutically useless, socially accepted substance with lame terpene smells that do not travel, have no flavor, and don't smell naughty whatsoever.



Sorry to derail but this post just as much to do with RKS as crossing a bunch of polyhybrid and praying to the weed gods that it'll come out pure skunk. Some people just can't see how jacked up this scene has become how misled everyone is. One big clue is the patron Saint of Skunk takes his namesake from a strain he stole, and doesn't like, oh and he coincidentally has tentacles all throughout the community, connections to agencies who specifically raid skunk growers, and once grew for a government sponsored pharmaceutical outfit that holds patents on Cannabis derivatives. The fact he's even allowed in this community keeps every Skunk grower on earth away from these data gathering forums.
 

nepalnt21

FRRRRRResh!
Veteran
no terpenes in chemdawg or sour diesel? not even remotely close to being true. chem (and hybrids like stardawg and onycd) and sd/ hybrids like sour jack always tick high on terp tests when i see the results.

pretty sure bubba and skunk have terps, pretty sure most non hemp cannabis sativa has terps
 

PDX Dopesmoker

Active member
…Cannabis breeders don't consult science specialists.

Cannabis breeders are specialist scientists. Twats with establishment degree mill licenses generally aren't scientific thinkers, they tend towards regurgitating the expensive dogma they're so proud of having memorized rather than making use of any grey matter they might have been born with.
 

Dankwolf

Active member
Ogkb 2.0 x amsestrial skunk #3

Ogkb 2.0 x amsestrial skunk #3

5 days before harvest

Her and my cottoncandy have a love date at some point

This one has the terps has structure and yield


picture.php



Will throw up some currant cottoncandy pics soon
 

Thesearch

Active member
There is something odd at play. It's the fact that Cannabis labs only test for terpenes,which have very little to do with natural Cannabis. And the fact that Cannabis breeders don't consult science specialists.

"It is not obvious how the mono- and sesquiterpenes in cannabis can be responsible for the skunk, diesel, and earthy notes observed in our samples. These characteristic notes may result from synergistic effects among terpenes or from the overlooked presence of non-terpene compounds" Dr Avery N. Gilbert
...

Without derailing this thread too much I implore you to take all perspectives into account here. Terpenes definitely do have medical and synergistic effects and I can prove this with many studies as well. Feel free to PM me for links. I suggest you join future 4200 where craft and industry producers and extractors discuss methodology, testing, terpenes and other individual compounds. More deep work has been done on these subjects there and I don't want to take away from this thread. There is certainly more at play in cannabis then terpenes, THC and CBD, ie. flavonoids, esters, alkanes, hormones, as well as many trace cannabinoids and many others that do not get discussed like various azeotropes of these compounds etc. Feel free to PM me for extended details.

@dankwolf
Looking good brother! The look of the bud is reminding me of that old school skunk look.
Breeding makes me happy to watch, keep the smoke reports rolling!
 

BigPhil

Well-known member
Veteran
Road Kill Skunk.

A cultivar known for the distinct odor of North American skunk spray,
once prevalent years ago, but lost.

I see could it of been a skunk x chem D.

A lot of good genetics have been lost of the years. Shame. But I bet some old timers have a few gems hidden away in their seed collection's
 

J-Icky

Active member
I see could it of been a skunk x chem D.

A lot of good genetics have been lost of the years. Shame. But I bet some old timers have a few gems hidden away in their seed collection's

The alleged RKS was from WAY WAY WAY before chem D was even a thing. But it’s possible the chem D has some RKS genes in it but without real genetic testing, not the bs Phyloswas selling as genetic testing, we will never know what chem/dog bud actually was.
 

BigPhil

Well-known member
Veteran
The alleged RKS was from WAY WAY WAY before chem D was even a thing. But it’s possible the chem D has some RKS genes in it but without real genetic testing, not the bs Phyloswas selling as genetic testing, we will never know what chem/dog bud actually was.

I was listening to a pod cast on YouTube couple of days ago. And they was talking about rks on that. They think it was an Afghan cross. Here is the link if anyone interested, They mention it within the 1st hour, Can't really narrow it down anymore than that

https://www.youtube.com/watch?v=VtWFFu6OMHw&t=10020s
 

Dankwolf

Active member
Interesting quest you are on. I would chime in that most folks for what ever reason, will not ever see even half the genetic potential if the soil is not balanced correctly.

The best varieties in the world are often discarded due to their unknown nutritional demands. Seen it way too many times in melons, snow peas, onions, even avocado.

I am running a small experiment with two clones from one OGK "supposedly". One is in Ca calculated at 85% plus gypsum spiked in with OM. A second clone, Ca only brought up to 68% Ca (using [email protected] not M3) which is where a number of "scientists" and I use the term loosely, choose to have their Ca due to lack of understanding.

The difference so far in genetic expression is quite clear. In the plant with the higher Ca, leaves 8 and 9 came out quickly. In the clone without the high Ca, those two leaves so far have not showed up.

If we are talking leaves, can you imagine the other genetics that will not be expressed?

In the produce industry we call the concept nutrient density. Often the term is not used correctly and folks try to pound with K to get nutrient density. Doesn't work that way. To reach the genetic potential the plant needs Ca. As do humans!

In Albrechts' work from the Univ of Missouri, he found that where there is more Ca on the west coast vs the east coast, that the US military soldiers were taller and had less cavities. The genetic potential was in the population, just the food supply didn't have enough Ca. Are the guys from the east coast genetically missing the height and tooth health gene? No. Just couldn't get what was necessary to express the genetics.

If Dank sees these traits and others do not, this may say more about growing techniques and soils than genetics....

Keep going Dankster. Many will never understand much less appreciate what you are trying to do. Don't let them get you down.... stay the course. Ignore ignorance. Don't waste your time nor effort.

Peace.


Had to repost this for motivation. Life has been tough but i am not giving up on this quest genetically/ or soil chemistry wise . i will get it

So a littel break down on my life . wife of 12 years is fucking some one els and drianed my bank account . and she has not worked in over a year . she is mia as of a few days ago . got to love life . so i am going to do what i do best and put my head down and keep working 12 gour days and handel garden ,pets and bills and wait for the to finish its course
 

Dropped Cat

Six Gummi Bears and Some Scotch
Veteran
Damn bro, but new challenge presents new solutions.

Had one like that years ago, don't take it personally,
she is moving on, you can move on too.

Going to take some adjustments, and not easy.

God bless, and good luck.
 

HAZENACIOUS

Member
Hi Dankwolf,
Gotta love the married life. You are not alone bro, women can be so cold, I've heard there are loyal ones out there somewhere... If it weren't for the dope, keeping me mellow, I probably would have ditched out by now (but I'm still bearing the yolk)


Buds are looking great, it seems you are on the right track.



Cool thread, I have experience with the original skunk bud from the 80s, wanted to share with you, maybe it can help. It has nothing to do with sk#1.
It was 85-88, I had the hookup, it was old school Humboldt County Death bud, smelled just like a skunk. Stunk through baggies, no way to hide the smell except with a film can. Everybody called it skunk bud. Anyway, years later I wound up hooking up with a network of old school growers in the Bay, a group that had been growing since the 60s, one of the older men, he told me that most of the original California kind bud could be traced to Big Sur, and spiraled out from there, a single 70s cross of Hawaiian sativas and a type of Afghan seed that was brought in large quantity by a hash smuggler in the 60s, he had purchased thousands of seeds from his connection in Afghanistan. I can't remember the name of the hash but it always came in red paper with a wax seal and was some of the best hash and was fairly common in California back in the day. The name of this hash is on the tip of my tongue, but I can't remember. I've actually smoked plenty of it, it was the most common of the really fine old-school Afghan hash.

Weed from this type of hash crossed with the best Hawaiian.

This was a very diverse cross but this cross is where the the old school giant California kind bud comes from, and there are 2 basic types over the years, the more popular and because of this, much more common type was the skunk which was Afghan dominant, and it was the most popular for its smell and resin content but mostly it was a giant Indica with huge buds and unheard of yields and ready in early September, sometimes before. The other pheno was also a giant and fairly early but more sweet with Hawaiian style buds, not as heavy or dense etc, but it was that special stock of Afghan dominant wide leaf plants that got massive like

Hawaiian but gave super skunky dense Afghan style buds with volume of fat Hawaiian buds, and this skunk line became the must have strain to grow because of its hardiness, yield, potency and wicked smell. It was so much heavier and more hardy, and superior than any other strain that it took over and was proliferated all over the northwest by the 80s, and didn't become a rarity until the Dutch weed took over in the 90s
 
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