Dankwolfs rks breeding project and breeding info discussion

[OregonBorn]

Was looking at getting some GA3 (gibberellic acid) to wake up some old tired seeds. However I read that it will also change the sex of Cannabis? Can you use GA3 on tired seeds to break dormancy and not do a sex change? And if so how?

 

[Cvh]

If I had gone to all the trouble to reverse males until I found one that was RKS when in female form and then it was time to breed that male I wouldn't be shy about violating the tenets of the breeding ethics religion by putting a female version of that male in the room when the pollen was being spread.

Wouldn't that then be a YY Karyotype? Are those seeds viable?

 

[zif]

Wouldn't that then be a YY Karyotype? Are those seeds viable?

Males donate X or Y chromosomes. Reversed males still donate X or Y chromosomes. So offspring would be 1/4 XX (female!), 1/2 XY (ordinary male), and 1/4 YY (super-males). I’m not sure if the latter are viable, but it’s very easy to test (1/4 of plants female? Yup, viable; 1/3? Not viable).

Either way, selfing a male starts a ‘regular’ seed line. Pretty cool.

 

[Cvh]

Ok. Thanks for explaining.

 

[Sam_Skunkman]

I have not grown enough YY seeds to be sure their viability is as high as an XY male seed, but I assume at least some are viable because they can be made. What should be used is a plant cytology lab that can test which is present cheaply, XY or YY.
If you make and grow enough and test them all you can see if they are 25% female XX, 50% male XY, and 25% YY. If the YY are not 25% of the total seeds I would suspect some of the YY were not viable and aborted.
Even without lab tests if you have enough seeds say 1000 to grow out and sex and you can see the 3/4 males are less then they should be, and then suspect the YY are not all viable.
-SamS

Males donate X or Y chromosomes. Reversed males still donate X or Y chromosomes. So offspring would be 1/4 XX (female!), 1/2 XY (ordinary male), and 1/4 YY (super-males). I’m not sure if the latter are viable, but it’s very easy to test (1/4 of plants female? Yup, viable; 1/3? Not viable).

Either way, selfing a male starts a ‘regular’ seed line. Pretty cool.

 

[zif]

Even without lab tests if you have enough seeds say 1000 to grow out and sex and you can see the 3/4 males are less then they should be, and then suspect the YY are not all viable.
-SamS

Making S2 lines might take fewer plants to show that some YY males are viable? Any all-male results would be from YY males...

Or, easy and fewer plants - cross suspected super males with a female. YY males will *still* make all males, but in this case all XY males.

 

[Miraculous Meds]

This seed run will be to look for rks profiles/terps to add to the project and show case the genetics stated below.I will be starting a thread just for tgis run soon . I will post up a link soon as I have the time .

A big thank you to mm for the genetics to benefit all.....

Seed strain #1. death star x( blue star f1 = ( death star x whitaker blues) .

Seed strain #2. ogkb 2.0 x Ancestrial skunk


Seed strian 3# . blue star x Ancestrial skunk

Big thanks to all that are helping with this life defining Project.

My Ancestrial crosses are on hold till I can finish sifting out the seed .

I will put a link to the mm thread soon .please,correct me,if I miss qouted genetic background

Looks good buddy, cant wait to see them up and running. Looks like you have everything right. I did misspell ancestral, but that's just a typo.



Did you get some of the death star x as as well? You should try and make room for that cross if you have it. I think she will be extra stinky.

 

[Dankwolf]

Looks good buddy, cant wait to see them up and running. Looks like you have everything right. I did misspell ancestral, but that's just a typo.



Did you get some of the death star x as as well? You should try and make room for that cross if you have it. I think she will be extra stinky.

I believe so .

Just got back from last min vaction (first one in 5 years ) . I will include all sent seeds . I am cleaning up grow area tomorrow in preparation for run . I do a in depth clean /sanitize twice a year not to mention the bimonthly routine wipe down / spray .

 

[Dropped Cat]

I believe so .

Just got back from last min vaction (first one in 5 years ) . I will include all sent seeds . I am cleaning up grow area tomorrow in preparation for run . I do a in depth clean /sanitize twice a year not to mention the bimonthly routine wipe down / spray .

Very good to see a grower reinforce the importance of a cleaning regiment.

Just as criticle as nutes, medium management, lighting, etc.

Excellent post.

 

[Dankwolf]

Rks project Update . the Ancestrial skunk x.........

Rks project Update . the Ancestrial skunk x.........

Bump

 

[Dankwolf]

The cure

The cure

 

[Dankwolf]

Rks project Update . the Ancestrial skunk x.........

Rks project Update . the Ancestrial skunk x.........

A big thank you to miraculous meds for his contributions .

1# i put seeds into a 220 grit sand paper envelope and shook them around for a min to prep for sproting.

2 # I rinsed all seed in a peroxide/ water solution at 1/10 then rinsed off with distilled ro water .

3# seed are now place in distilled ro water in solo cups that have been bleach sterilized / rinsed thorughly with distilled ro water

4 # seeds will soak for 24 hours in said solo cups of water at 70° .

5 # I will be placing seeds in clean non-bleached paper towel for 1.5 - 4 days then will be place in a seed starter mix in 4 in potters.

Miraculous meds strians ( 10 of each and will be labeled 1-10 should all of 10 of each strain pop)

Seed strain #1. death star x( blue star f1 = ( death star x whitaker blues) .

Seed strain #2. ogkb 2.0 x Ancestrial skunk


Seed strian 3# . blue star x Ancestrial skunk

Seed strain 4# death star x Ancestrial skunk

Mr nice strains ( only starting 5 of these should all pop they will be labeled 1-5 ) .

Seed strian 5 # "the cure " afghan/skunk x Haze AC

I am sure I missed something . so if you have any questions please ask ?

Also I will start a thread just for this seed run soon as it gets interesting.

 

[Dankwolf]

Been out awhile due to mite brought in fro the out door .

Been out awhile due to mite brought in fro the out door .

Been having a rough time lately . i honestly hate out door but it keeps my wifr in smoke so I can play indoor. Lol
She is a 1/4p- 1/2p a month yup of girl lol ( I give alot to my family as well ). Any way my mothers are looking sad hence the lack of updates lately .

My sad mothers ( mite free finally).

The mite culprits . ( trimmed and un trimmed ) need more room lol.

1 tote down lol

Over flow of un-trimmed in shower lol moths to go the rest of un trimmed are in dark I hate to love this time of year .

 

[Miraculous Meds]

A big thank you to miraculous meds for his contributions .

1# i put seeds into a 220 grit sand paper envelope and shook them around for a min to prep for sproting.

2 # I rinsed all seed in a peroxide/ water solution at 1/10 then rinsed off with distilled ro water .

3# seed are now place in distilled ro water in solo cups that have been bleach sterilized / rinsed thorughly with distilled ro water

4 # seeds will soak for 24 hours in said solo cups of water at 70° .

5 # I will be placing seeds in clean non-bleached paper towel for 1.5 - 4 days then will be place in a seed starter mix in 4 in potters.

Miraculous meds strians ( 10 of each and will be labeled 1-10 should all of 10 of each strain pop)

Seed strain #1. death star x( blue star f1 = ( death star x whitaker blues) .

Seed strain #2. ogkb 2.0 x Ancestrial skunk


Seed strian 3# . blue star x Ancestrial skunk

Seed strain 4# death star x Ancestrial skunk

View Image View Image

Mr nice strains ( only starting 5 of these should all pop they will be labeled 1-5 ) .

Seed strian 5 # "the cure " afghan/skunk x Haze AC

View Image


Whoah! Great start on these babies, thanks for giving them a go. Hoping for some serious skunk aromas.


Looks like you have a good clean germination planned out. You may already do this, but id recommend changing your paper towels every day to avoid mold. Its well worth the effort to make sure the paper towel doesn't grow anything we don't want, besides the seed.


Heres to a great hunt my friend! I will be eagerly following the journey.

 

[Dankwolf]

Whoah! Great start on these babies, thanks for giving them a go. Hoping for some serious skunk aromas.


Looks like you have a good clean germination planned out. You may already do this, but id recommend changing your paper towels every day to avoid mold. Its well worth the effort to make sure the paper towel doesn't grow anything we don't want, besides the seed.


Heres to a great hunt my friend! I will be eagerly following the journey.

I plan to swich paper towel out every 24 hours. I have had problems in the past if not changed out at least every 2 days .. I also try not to touch seeds or paper towel with my hands .

 

[Dankwolf]

24 hour soak results

24 hour soak results

Seed strain #1. death star x( blue star f1 = ( death star x whitaker blues) .

All 10 seeds sunk after 24 hours . 2 seeds have broke there shell . ( side note seeds have a s1 appearance) defiantly can see the back cross in these . (side note I am expecting less of a genome expression time will tell .



Seed strain #2. ogkb 2.0 x Ancestrial skunk

------- all seed sunk after 24 hours . 2 of 10 are showing tails.

Seed strian 3# . blue star x Ancestrial skunk

------ 9 out of 10 sunk after 24 hours . 3 out 0f 10 are showing tails.

Seed strain 4# death star x Ancestrial skunk

------ All seeds sunk after 24 hours .3 out of 10 are already showing tails

Seed strian 5 # "the cure " afghan/skunk x Haze AC

-------- All 5 seeds sunk after 24 hours . no tails yet .

 

[Dankwolf]

Solo cups half full of seed Starter mix

Solo cups half full of seed Starter mix

I fill solo cips half full so I can back fill with a bit richer soil as they grow and prevent lanky plants with weak root growth .

All seeds planted . lost a dew for a few different reasons mainly helper error .

 

['Boogieman']

Just curious, was this roadkill skunk brick weed from mexico? I had some foul skunky brickweed in the early 2000's I believe, you couldn't get rid of the skunk smell if the bag was put there, my friends always thought I had weed in my glovebox when it was empty.

 

[Dankwolf]

Just curious, was this roadkill skunk brick weed from mexico? I had some foul skunky brickweed in the early 2000's I believe, you couldn't get rid of the skunk smell if the bag was put there, my friends always thought I had weed in my glovebox when it was empty.

I believe the true rks was a Colombian gold pheno of skunk 1 or a Columbian gold project that knocked,up a afghan of a sweet/meaty profile and selected through many circle's .

there were for sure more then one rks . the one most speak of is thought to be from oregon/Cali "trinity" . me personally I don't think so . I grew trinity in early 2000 and she had it in her but it was not pure rks that I look for
( I think it originated in Washington , I think it may have been part of the original northern lights projects or possibly a shot off of a nl project time line fits .

Were I am lost is the fact I have found many rks terps in mazar-I-Sharif , and African sativa dated 2000-2005.many of the original rks plants exshibited mazar traits .

Take it for what you want that is my thoughts and direction

 

[GoatCheese]

I believe the true rks was a Colombian gold pheno of skunk 1 or a Columbian gold project that knocked,up a afghan of a sweet/meaty profile and selected through many circle's .


[...]

Were I am lost is the fact I have found many rks terps in mazar-I-Sharif , and African sativa dated 2000-2005.many of the original rks plants exshibited mazar traits .

Take it for what you want that is my thoughts and direction

Hi.
If you're wondering what gave Skunk#1 the skunky smell, it was the Mazari Afghan Sam used in the project. I already posted the quote from him on page 3 of this thread.


Also, if you've found skunky aromas from African strains, there's some Pakistani/N Indian genetics in Africa, according to Phylos Galaxy, which could explain this. Alot of Indians went to Africa to work during the British rule, Gandhi being one of them.
..atleast some Durban genetics look like genetics from Chitral, Pakistan.



Here's Sam about the Afghan in Skunk1

Q: "sam...was the afghan or columbian you used really skunky as hell to begin with?"

The Afghan was.
-SamS

https://www.icmag.com/ic/showpost.php?p=1865299&postcount=502


Q: "From which region of Afghanistan did the 'Afghan' in 'Skunk #1' come from?"

Mazar, but then crossed with a Columbian, then selfed, then the hybrid crossed with Acapulco Gold to make it a bit earlier.
But to be honest in the same seed batch from Afghanistan you will find many phenos, remember they may have thousands of females that the seeds came from, as well as thousands of males hitting every one of the females, so that is a lot of possible combos.....

[...]
-SamS

https://www.icmag.com/ic/showpost.php?p=1528952&postcount=163