:D Genetic Preservation :D - Breeding

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Sesquiterpene lactone - Wikipedia

en.wikipedia.org

Putrescine - Wikipedia

en.wikipedia.org

Putrescine production in plants can also be promoted by fungi in the soil.[8] Piriformospora indica (P. indica) is one such fungus, found to promote putrescine production in Arabidopsis and common garden tomato plants. In a 2022 study it was shown that the presence of this fungus had a promotional effect on the growth of the root structure of plants. After gas chromatography testing, putrescine was found in higher amounts in these root structures.[9]

Plants that had been inoculated with P. indica had presented an excess of arginine decarboxylase.[9] This is used in the process of making putrescine in plant cells. One of the downstream effects of putrescine in root cells is the production of auxin. That same study found that putrescine added as a fertilizer showed the same results as if it was inoculated with the fungus, which was also shown in Arabidopsis and barley. The evolutionary foundations of this connection and putrescine are still unclear.

Putrescine is a component of bad breath and bacterial vaginosis.[10] It is also found in semen and some microalgae, together with spermine and spermidine.

Arabidopsis - Wikipedia

en.wikipedia.org

Arabidopsis (rockcress) is a genus in the family Brassicaceae. They are small flowering plants related to cabbage and mustard.

Serendipita indica - Wikipedia

en.wikipedia.org

discovered from orchid plants in the Thar desert in Rajasthan, India

S. indica can be easily grown on various substrates. It has been found to promote plant growth during its mutualistic symbiotic relationship with a wide variety of plants.[1] Experiments have shown that S. indica increases the resistance of colonized plants against fungal pathogens.[2

https://apsjournals.apsnet.org/doi/10.1094/PHYTO-97-4-0523

 

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Brazilian Seed Co.s Manga Rosa Description​

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Pure Brazilian sativa from the 70s, a classical around here and all the country too. Never was hybridized. Very nice and long lasting high, natural high for clean cerebral activity, delicious taste and bouquet of sweet mango fruit, fat long fox tail buds full of resin with orange to light red hairs.

This strain was used to breed White Widow.
Grows as a main stem with few side branches, better growing outdoor but indoor does fine too.

Shes performs well indoor cause she tends to produce few side branches and this is great when you don't have much space to crowd then together. Doesn't need much of attention and care, keep her very well irrigated with balanced nutrients and sufficient nitrogen when flowering, big fat and long buds should be expected.

Height: 2 meter
Flowering: 10 to 12 weeks
Yield: 300 grams

Aracaju Red	150
Pernambuco Gold	150
Santa Marta Colombian	150
Piff	110
Angola Red	100
Raxa Coconut	98
Bahia Blackhead	95
Amarelo	91
Local Sativa	84
Original Green	84
Trinity	80
Brazilian Haze	77
Brazilian Lemon	77
Manga Rosa	77
Choco Chiba	75
Golden Sativa	74
Super Brazil	74
Super Kimo	74
Colombian Haze	70
Early Chemo	70
Tropical Treat Special	70
Colombian Gold	67
Chemo X Jack Herer	63
Colombian Jack	63
Early Green	63
Green Medicine	63
Brazilian Skunk	60
Super Green	60
Early Girl	56

 

[Fillthavoid]

Read his ic info the thread on parental plants!
Not sure what DP has but id prefer the reg as well. in usa multiverse has seeds from dp
dude over there is cool!

I like tivas up motivational vibe, the dj strains are tasty!
One of the prettiest strains imo, bb nice nugs
I would def do the flo again, let me know if you find some regs
Compare some notes on those
Didnt you find some Purple Thai some where OPT

I bred a highland oaxcan gold (purple pheno) x snowhighs old skool chocolate thai ( old oregon chocolate thai) which means im going to hunt thru them and find some Oregon purple thai without the mutation in the leaf. Very stable and strong male and female.


On a side note... AK Bean Brains vintage blueberry is the best blueberry ive grown yet since the dabney blueberry we used to have back on the boards in the 2000s. If you are wondering who that was you can remember my brother and I were scaybeez. RIP to my bro.

 

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#24 K is for keeper

 

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SSH Photo By:Swifty

 

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Picture from: Guest1 Underground Seeds Collective Colombian Gold 72

 

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neutral (black box) and deleterious (red box) mutations

Arcata Trainwreck | Kannapedia

www.kannapedia.net

CGAT - Computational Genomics Analysis Tools — CGAT documentation

Galaxy

Galaxy is a community-driven web-based analysis platform for life science research.

usegalaxy.org

Tangie ​

Punta Roja ​

Purple Columbian ​

A5 Haze ​

C5 Haze ​

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How to read your Kannapedia® Report

This article will help customers to better understand what they are getting in their Kannapedia® report and where to locate said information.

help.medicinalgenomics.com

 

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Origin and Evolution of the Cannabinoid Oxidocyclase Gene Family - PMC

Cannabis is an ancient crop representing a rapidly increasing legal market, especially for medicinal purposes. Medicinal and psychoactive effects of Cannabis rely on specific terpenophenolic ligands named cannabinoids. Recent whole-genome sequencing ...

www.ncbi.nlm.nih.gov

 

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HP13 '96 (THAI X LEB) g13

 

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View attachment 18954128

What is the source of this diagram?

Says , "H5-H19 is homogeneous gene pool" and H20 Polyhybrid "AB x CD" = Landrace "AB x CD."

"Landrace strain with fully stabilized traits "20 generations of line breeding is generally considered to be adequate for landrace status" Homezygous for selected traits.

 

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BREEDING BETTER CANNABIS
October 1 2005 Subcool

BREEDING BETTER CANNABIS
COLLECTING ELITE STRAINS
Subcool
The creator of such pot varieties as Jack’s Cleaner and Danny Boy reveals the secrets to spawning your own sensi strains.
Many people collect things as a hobby. MzJill and I are no different, except we collect elite strains of cannabis—usually in clone-only format (i.e., available only as cuttings and not as seeds). Over the past 25 years, I've acquired and grown out hundreds of strains and attended the Cannabis Cup in Amsterdam seven times, even living in the Netherlands for a while. I still believe, however, that the best cannabis in the world is in North America, in some unknown garden cultivated by someone without Internet access.
We've been lucky enough to collect some amazing examples. A few of my all-time favorites are Genius and Apollo 13 by Brothers Grimm, as well as Killer Queen and Space
Queen from Vic High. Our most potent strain is Jack's Cleaner, a Pluton x Lamb's Bread x Purple Haze mutant that can literally make a non-grower puke if he's not used to its brutal, raw potency.
MzJill has some of her own favorites as well. The Black Russian strain that gives all of our Sputnik crosses their insane purple coloring came to MzJill from master grower NEBU. Over the past five years, we've worked with these varieties to create new and interesting breeds of pot. MzJill comes up with her own crosses using her favorites and helps me pollinate when the males are ready.
Breeding cannabis takes instinct and experience. Any person can indiscriminately cross a male and a female plant, producing seeds with many variations. It may stilt be possible to find a diamond in the rough, but a true breeder makes his (or her) decisions based on a disciplined selection process for the parents. By growing out many specimens and choosing only the best selections for males and mother plants, breeders improve their stock and make significant advances in ganja genetics.
WHEN TO BREED
While timing pollination is not an exact science, it's important to know the attributes of the male and female strains used in breeding projects. Learn the various flowering times and traits of your parent plants and you'll know when to impregnate each one for peak seed production.
Most males begin blooming and produce sex organs faster than females. With this in mind, we place the selected female plant into our flowering room seven days before the chosen male. With a head start in the 12hour-on/12-hour-off lighting cycle that forces pot plants to blossom, the mother will be ready for pollination just as the male matures. Always keep males separate from other fertile females; only the mother-to-be should be in the vicinity, to prevent random crosses and unintentionally seeded crops.
A month or so into the flowering cycle, the selected parents are prepared to mate. A wellplaced fan will help disperse the pollen, but we also carefully remove mature male stamens and lightly sprinkle the grains onto female buds to ensure insemination. After three or four days of intimate contact, kill the male and gently rinse the female with water to assure that all the pollen is either activated or washed away before any contact with other flowering plants.
Within a few days, the hairs on the pregnant female will begin to change color and the calyx will start to swell with seeds. When the bracts have opened, exposing mature seeds, you're ready to harvest. Cut down and hang the plants over large roasting pans until they're about 75% dry. Then simply squeeze the buds so that the seeds fall out into the pans. Label and store the seeds in a cool, dry place.
INTENT TO BREED
Our goal is quite simple: to combine an extremely potent sativa hybrid with a heavyyielding, ultra-purple indica variety. Our hope is that the result will be a short-flowering purple strain with potency and flavor added by the sativa parent. Predicting the outcome isn't quite so easy, however, and many factors need to be taken into account.
Some strains, such as G-13, are extremely dominant and can take over a cross. When you combine Blueberry and G-13, you're likely to get a nasty-tasting, watered-down version of both. This doesn't mean there aren't good Blueberry/G-13 crosses, only that they're few and far between. Parents with distinct differences are essential to achieve hybrid vigor in the first filial generation (F1 ), so choose parent strains wisely.
THE MOMMAS & THE POPPAS
Selection of the mother plant (or P1) is easy.
Apollo 13 is one of my top-three favorite varieties and our most unique strain. Its combination of intense potency and a bizarre, putrid rotting-fruit smell truly makes Apollo 13 one of a kind. It possesses a soaring buzz that can actually cause anxiety if too much is smoked.
The choice of the male pollen donor isn't quite so simple. The first mistake people make in selecting a male is to choose the most vigorous specimen. Potency is not evident in the strength and speed of a plant’s growth. In fact, the earliest-maturing males are very likely to be less potent than later ones. This is because the natural tendency of cannabis in the wild is to revert to less potent "acclimatized” varieties.
As Robert Clark states in his seminal book on breeding, Marijuana Botany, "The only way cannabis varieties ever get better is through human intervention in the natural order.”
In order to find the recessive trait of poten-
cy, we pick males based loosely on the following several criteria. At the time of germination, are there any capitate trichomes on the cotyledon leaves? Another great indicator of future resin production is the number and frequency of non-glandular trichomes on later-flowering males. The males showing flowers first are tossed (dominant males are useless for our purposes, and their inclusion in amateur breeding programs has caused more hermaphroditic strains than the world deserves). If these basics are adhered to each time during the male-selection process, we have a much better chance of finding fantastic genes.
Sometimes taste is the desired goal rather than potency. I've taken cherry-flavored Ortega and crossed it with Killer Queen to create a cherry phenotype called Danny Boy. It finishes after 48 days of flowering and tastes just like cherry candy. Our famed Jack's Cleaner was crossed with Blueberry to create Batgirl. The Jack's Cleaner x Blueberry male has blue pollen sacs and drips with resin. Though all
of these plants are heady and strong, it's their flavors and odors that distinguish them, making the buds unique and interesting. Ultimately, we chose Black Russian to be the male pollen donor for our new cross.
Also in the works is an Orange Velvet/Space Queen cross that will be our first pineapple-orange offering. This is MzJill's creation, so we've named it Jillybean. MzJill's an extremely accomplished grower, but she only recently began breeding. Hopefully, with the right guidance and choices, Jillybean will turn out better than anything we've created yet.
LAUNCHING SPUTNIK 1.0
Now that our Black Russian/Apollo 13 cross was completed, it was time to give it a name. Sputnik seemed like a logical title.
We grew out many seeds and found that there are two distinct phenotypes. The first—truly eye-candy, with pink calyxes and oozing resin—we called Pinky. We knew instantly we had achieved our desired results: It smells like blackberry jam and plumps up to a wonderful large cola covered in so much THC, I'll never tire of photographing this beauty. At four weeks, the pink colors started changing to magenta, and even the trichomes took on a pink-topurple coloring.
The other phenotype, Whitey, was a surprise to me and also a valuable learning experience. Whitey exhibited no purple coloring; the plant was ultra-white, and during flowering smelled almost like a cream soda. As Whitey matured, it took on a weird, pungent smell. Before it cured out a bit, MzJill and I both thought it smelled pretty foul. It was only after two weeks of curing, however, that I decided to give it another chance. The result was a super-potent smoke that had me floored. A grower friend who sampled it said it almost gave her visuals. Now that we knew our cross had potential, how could we improve on it again?
BACK-CROSSING
The concept of back-crossing is also simple in theory: You pollinate your original P1 mother plant (in this case, Apollo 13) using the male offspring. That may be unusual in our world, but plants respond to it favorably. Selecting a male from the Sputnik 1.0 cross, we then pollinated the Apollo 13 mom using the chosen male. The first cross can be explained as a 50/50 split, while the second is 25/75, with 75% of its makeup coming from the Apollo 13 mother. That 75% cross, or Sputnik 2.0, is now complete and available to the public.
You don't have to stop at three crosses—you can always do it again and see what happens. We have a different plan, though. Once Sputnik 3.0 is done, we'll then grow out 100 seeds and select 10 males and 10 females. The best will be used to create Apollo 13 cubed; these seeds will be even more stable and extremely valuable, representing thousands of hours of work. They can be used to create true breeding IBLs (inbred lines), or to find more P1s for future breeders to incorporate into their genetic pool. Team Green Avenger and Subcool continue to strive for the best of the new crosses in the search for recessive genetics. ^
Subcool, MzJill, SunyCheba and BadBoy (a.k.a. Team Green Avenger) can be found on Overgrow.com. Subcool seeds are available from Dr. Chronic at drchronic.com and Heaven's Stairway at hempqc.com.
BREEDER'S GLOSSARY
Allele - One member of a pair or series of genes that occupy a specific position on a specific chromosome.
Back-crossing - Breeding an individual plant with its own offspring.
Biosynthesis - Production of a chemical compound by a living organism.
Calyx - The sepals of a flower considered as a group.
Cubed - Your third back-cross, where you take the progeny (squared) from the second back-cross and cross back to the same parent (the great-grandfather now).
Cotyledon leaves - A leaf of the embryo of a seed plant which, upon germination, remains in the seed or emerges, enlarges and becomes green (also referred to as the seed leaf].
Dominant - Said of an allele which, by itself alone, will produce a particular phenotype regardless of whatever other allele may be present on the other matching chromosome of the diploid pair. It takes only one copy of the chromosome to cause a dominant trait to be expressed in the phenotype.
F1 generation - The progeny produced from a cross between two parents (PI ) is called the first filial or F1 generation.
F2 generation - The progeny resulting from selfhybridization or inbreeding of F1 individuals is called the second filial or F2 generation.
Genome - The total genetic information possessed by an individual, breed or species.
Genotype - The invisible genetic makeup of an individual organism, including alleles that may be recessive and therefore have no visible physical expression.
Hermaphrodite - A plant that has both male and female reproductive organs.
Homozygous - An individual possessing (receiving from its parents) identical alleles for a trait is said to be homozygous or pure for that trait (e.g., a plant with RR alleles is homozygous for the seed shape). A homozygous plant always breeds true for that trait.
Hybrid - The offspring of genetically dissimilar parents or stock, especially the offspring produced by breeding plants or animals of different varieties, species or races.
Inbred Une (IBL) - A line produced by at least five generations of sequential inbreeding, self-fertilization or back-crossing, accompanied by selection within and between lines so that the individuals are considered to be homozygous, or nearly so.
P1 - The name of the parent to which a hybrid is crossed in a back-cross.
Phenotype - The external appearance of an individual for any trait or traits (e.g., for seeds, round or wrinkled are two examples of a phenotype).
Recessive - Producing little or no phenotypic effect when occurring in heterozygous condition with a contrasting allele. Expressed only when the determining gene is in the homozygous condition.
Recessive trait - A genetically determined characteristic or condition.
Trichomes - Cannabis resin glands. They come in three basic varieties. The bulbous type is the smallest. Nextlargest is called capitate, with a globular-shaped head. The most abundant are capitate-stalked glands, which consist of a tier of secretor disk cells subtending a non-cellular secretor cavity.

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canna bible polly
big sur

• Big Sur Holyweed Phenotype: Zacatecas Purple Pheno

The female used in this hybrid was Big Sur Holyweed (Zacatecas Purple Pheno) passed down from renowned mystical Pot-ologist NDNGuy. It's a big massive sativa with HUGE buds, however like most wild mexican sativas, it had lower resin production but packed a huge wallop.

 

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Big Sur Hophead Harvest
October 1 1976 Ma, Pa Kettle


Big Sur Hophead Harvest
Ma and Pa Kettle grow their own dynamite domestic
Ma and Pa Kettle
A couple years back, we decided to hang up our nine-to-five east coast routing and go back to the earth in points west, We camped our way through every scenic place imaginable and got really hung up on the California Sierras. But we thought we’d check out the Big Sur coast before four-wheeling it through the forests in search of a likely site
for a homestead.
Within a month, we found ourselves looking out over the Pacific Ocean from a primitive shack among the coastal redwoods. We were awed with nature and high on life.
Fresh eggs and homegrown vegetables were top priorities on our planning list, and in that remote area another kind of homegrown quickly came to mind. I picked up a copy of a popular grass cultivator’s handbook and used an organic gardening encyclopedia for e tine points !ompletely absent. Th~roubIe w,L.~
and loadiest plant used to selectively pollinate the branchiest of the females when they reach full maturity.
Out of the hundred plants we grew, about 50 were females, and some of them yielded over a pound per plant. Our best ounce sold for $ 1 7 5, and we were party to a pound or two that went for $2,000.
A lot of the fun in growing is in the experimenting and the learning. Don’t do the same things with each plant. Pruning techniques, amount of water and nutrients, and methods of harvesting, alone, will produce huge variations in the end product. Above all, be sensitive —spend time with your plants exchanging good energy. Get the feeling and joy of getting back to the earth.

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edits*

Big Sur

BSHW is an old school heirloom sativa from the 70's (central coastal california).
used/available from from perry the monk, reeferman, danbo and hhf.

 

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llanos orientales

Santa Marta

Aracaju Red sister manga rosa

Pernambuco Gold​

WHP

 

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The Primary Colors of Cannabis​

The first modern cannabis hybrid was created in the summer of 1969 in the Santa Cruz mountains by a surfer we only know by his first initial “G”, and legend has it that he crossed together three varieties from Colombia and created what the world has come to know as Original Haze.

byTodd McCormick
June 6, 2023

This was a significant milestone for cannabis because during the 1960s, the majority of cannabis that was coming into the United States was coming from tropical regions such as Vietnam, Laos, Cambodia, Thailand, Colombia, Mexico, and Jamaica and were designated by names that indicated where they were grown such as Colombian Gold, Acapulco Gold, Michoacán, and Thai Stick.
Starting in the late 50s and going through the late 70s, people from the counterculture started navigating their way from London to India on what has become known as the “Hippie Trail”.
These traveling adventurers would play a significant role in the evolution of cannabis varieties because as they would travel through Afghanistan, they would pick up and bring back ancient varieties of cannabis that had long been bred for hashish and had broad leaves and short flowering times. Incorrectly identified as “Indica” because the leaf morphology was so different from the more common narrow leaf “sativa” varieties originating in the tropical/equatorial regions listed above. Afghan cannabis is so unique to the other varieties around the world that it has been argued that it should have its own classification.
Please see my article: Ditch the Old Terminology (An Indica / Sativa Response)

Original Haze / Courtesy Todd McCormick
Further east on the trail was India, where true “Indica” grows. In India, cannabis leaf morphology ranges from broad leaflet varieties in the north, to very narrow leaflet varieties down south and along the coast. I recently received seeds from Manipur India, which have been grown in the region for their drug content for thousands of years and they are the smallest seeds in my collection at 101 seeds per gram. Comparatively, “Purest Indica” and Northern Lights #2 are large and have approximately 38 seeds per g, followed by Northern Lights #5 and Skunk #1 at 50 seeds per g, and some of the smallest are Original Haze at 70 seeds per g.
One of the hippies who took the Hippie Trail back in the early 70s was Skunkman Sam and his wife of now 53 years, who hitchhiked from London to Morocco, and from Morocco back up to Germany, through Afghanistan and across to India, where they lived for over a year. Along the way picking up varieties of cannabis and saving the seeds.

When Skunkman Sam arrived back in California after his time in India, he just happened to move next to the breeder of Original Haze. Sam realized that the original combination of Haze was unique and he kept it isolated away from his other varieties so that it would not experience genetic drift, which would cause the loss of the variety over time.

Comparison between Original Haze seed and Northern Lights #2 / Courtesy Todd McCormick
During the early 70s, Skunkman Sam started breeding together some of the varieties he brought back on his journey along with some of the varieties that were being imported into California and created what would become known as Skunk #1, which is a combination of (Afghan x Colombian Gold) x Acapulco Gold.
Skunk #1 would revolutionize cannabis cultivation because it was a relatively consistent and uniform variety that had incredibly high-quality buds and flowered in a short amount of time. Fortunately, or unfortunately, depending on who you ask, the initial rendition of the variety favored the Afghan which was very pungent and smelled similar to a skunk scent, hence the name “Skunk #1”. This new variety which would harvest in late August compared to November or December for Haze, quickly took over as the favored variety by many growers.
We now understand that this funky acrid scent is caused by thiols which humans can detect in parts per trillion. Usually it is a scent that is released when food is going bad and it is a clue for humans to avoid it. Since the 80s, many breeders have selected away from plants with thiols and favored the terpenes which are responsible for the sweeter side of cannabis.
In 1976, Skunk #1 seeds were released by the first modern seed company which was started by Skunkman Sam and called “Sacred Seeds”. Sam would go on to collect varieties from the likes of Mel Frank and other lesser known cannabis cultivators to create one of the greatest genetic collections imaginable.

Skunk #1 / Courtesy Todd McCormick
In 1979 Sam authored an article titled “Sun, Soil, Seeds and Soul” for an LSD magazine called BLOTTER. The article was focused on educating the readers about breeding the best characteristics of cannabis. When I asked Skunkman Sam how he was able to breed Skunk #1 to be relatively true breeding, he very humbly said, “I didn’t, nature did. I just had to cross together and test enough plants until I found the combination that worked.”
In the mid 70s, Afghan varieties started becoming quite popular with growers who realized the advantages of an early harvest and heavy yield. Some of these Afghan seeds made their way up to the Pacific Northwest and became known as “Purest Indica” and “Hash Plant” eventually became the backbone of what we now know as the “Northern Lights” family of crosses.
The NL hybrids were created in the early 80s by a group of friends around a former Marine and Vietnam veteran nicknamed Seattle Greg. Greg told me that in 1979 he received just 4 seeds of the “Purest Indica” from the author Murphy Stevens, who published one of the most advanced cultivation books in the mid-70s titled: How to Grow Marijuana Indoors Under Lights.
Greg crossed these four seeds together as an IBL or “in-bred line” and passed the seeds that he made out to his friends who then out-bred the “Purest Indica” with the varieties that they were already growing. They shared some of their seed creations back with Greg, who grew them out and then numbered them from #1 to #11 based on #1 being the closest morphology to the “Purest Indica” and #11 being mostly tropical or narrow-leaf. The ever popular Northern Lights #5 was in the middle with a nice balanced combination of both the “Purest Indica” and equatorial genetics.

Seattle Greg standing next to Northern Lights plants / Courtesy Todd McCormick
In 1984, the work of these two breeders and collectors would converge in an unusual way when a heroin addict named Nevil used a grant from the Dutch government to get clean by growing cannabis seeds and starting a company that he called The Holland Seed Bank.
Nevil was inspired to start growing after reading a Mel Frank book in the early 80s and by 1984, he was growing indoors under lights, but not having the greatest success with the seeds available to him. It is interesting that Nevil would come to be regarded as a great breeder because if anybody started growing cannabis and then less than one year later, started a seed company, I don’t think anybody would think they were all that experienced as a breeder, but in the 1980s, it was easy to take credit for other people’s work and that’s exactly what Nevil and many others in the Amsterdam seed business did. Amusingly enough, I have often had to remind my European friends that there are no skunks in Europe and that none of them would know what one smelled like unless they visited the United States because a skunk is a North American animal.
Initially Nevil started collecting seeds from the local coffeeshops around the Netherlands and sold the seeds for just $.50 each with the disclaimer that he could not vouch for the quality of the seeds because he didn’t grow them, but that would all change when Nevil was introduced to Skunkman Sam and Seattle Greg who supplied Nevil with the genetics that they and their friends had been working on for years.
In 1984, Nevil acquired from Skunkman Sam Skunk #1, Hindu Kush, Afghan #1, Durban Poison, California Orange, Early Girl and many others. Later in 1987, Nevil would also be given a couple Haze males from Skunkman Sam that Nevil used for breeding, and hybrids with Haze first appeared in The Holland Seed Bank’s 1988 catalog.
In 1985, Nevil acquired the Northern Lights hybrids #1 through #11 from Seattle Greg, but he did not get the “Purest Indica” which is the parent variety of all Northern Lights hybrids because Greg did not want Nevil to be able to make the Northern Lights hybrids without him.

Northern Lights #2 leaf morphology is crazy / Courtesy Todd McCormick
Immediately after receiving these classic varieties, Nevil started crossing them together and creating secondary and tertiary colors such as Northern Lights #5 x Haze, NL#5 x Skunk #1, Silver Pearl which was Early Girl x Skunk #1 x NL#5 and many others.
While The Holland Seed Bank was not the first to sell cannabis seeds, it quickly became one of the most significant because it was the first seed company to advertise in High Times Magazine and sold seeds to America, which was not only the largest market, but also the most dangerous because seeds were completely illegal at the time in the USA. In March of 1987, High Times ran an extremely long and personal article interviewing Nevil, and focusing on The Holland Seed Bank which made what he was doing instantly legendary to the rest of us reading through the pages of the world’s only cannabis magazine at the time.
In the years that followed, this relatively small group of varieties would become the foundation of many of the modern hybrids that we have today. When science started looking at the DNA structure of cannabis and comparing the relationships of many of the cuttings that are commonly cultivated throughout California and many legal states, they realized that almost all of them were related and had either Skunk #1 or Northern Lights genetics, or in many examples, both.
Fortunately, these primary colors were tucked away in freezers and refrigerators and, decades later, I was honored to receive many of the same genetics shared with The Holland Seed Bank in ’84 & ’85 such as Afghan #1 and Durban Poison from Mel Frank, Skunk #1 & Original Haze directly from Skunkman Sam and “Purest Indica”, Northern Lights #2 & Northern Lights #5 directly from Seattle Greg, which are currently available through my seed company:

 

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[Mimpi Manis]

You are turning in some informative, intelligent post's here my friend. Nice work.