acespicoli
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Hemp Husbandry
Robert A. Nelson
Copyright 1999 ~ All rights reserved.
Table of Contents
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:D Genetic Preservation :D - Breeding
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Robert A. Nelson
Copyright 1999 ~ All rights reserved.
Table of Contents
acespicoli
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What happens if you have tetraploid?
It is usually associated with miscarriage. Tetraploidy is an extremely rare chromosomal anomaly, polyploidy, when an affected individual has four copies of each chromosome, instead of two, resulting in total of 92 chromosomes in each cell.
It is usually associated with miscarriage. Tetraploidy is an extremely rare chromosomal anomaly, polyploidy, when an affected individual has four copies of each chromosome, instead of two, resulting in total of 92 chromosomes in each cell.
acespicoli
Well-known member
Tetraploid cannabis plants are similar to triploids in the sense that they are also polyploids. However, instead of three sets of chromosomes, each cell possesses four—two sets from each parent. These varieties are somewhat easier to create than triploid ones; you can view them as a botanical precursor.
acespicoli
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Tetraploidy is formed from diploid cells through mechanisms such as cell fusion, endoreduplication, mitotic slippage, or cytokinetic failure, the latter two being the main routes (Figure 1).2, 3 Mitotic slippage is a phenomenon in which mitotic cells enter the next cell cycle without undergoing chromosome segregation ...
acespicoli
Well-known member
Mitotic slippage is a phenomenon in which mitotic cells enter the next cell cycle without undergoing chromosome segregation ...
Imagine tetraploid male strain...
Naturally occuring what are the odds?
Something is producing natural triploid sterile females...
Seed treatment In the second experiment, different concentrations of colchicine, oryzalin and trifluralin were used to treat the seeds by soaking them in the antimitotic solutions for 6, 12 or 24 h. In each treatment, 50 seeds were used, and Tween “20” was added as surfactant for better efficiency of the treatments. The treated seeds were washed with distilled water and planted in the trays filled with peat moss
erlite:soil (1:1:1) in greenhouse under 16 h light period, 27–24°C day/night temperature and 63% humid- ity. This experiment was arranged in a factorial based on a completely randomized design with five replications. Seedling treatment In the third experiment, the seeds were planted in plant- ing trays. At the emergence of two true leaves stage, the seedlings were immerged in the above mentioned con- centrations of colchicine, oryzalin and trifluralin for 6, 12 or 24 h. In each treatment, 50 seedlings were used. The treated seedlings were washed with distilled water and carefully planted in the pots filled with a mixture of leaf mold:sand:loam soil (1:1:1). The pots were put in greenhouse with 16 h light period, 27–24°C day/night temperature and 63% humidity. This experiment was a factorial arrangement based on a completely rand- omized design with five replications. Flow cytometry Ploidy was assessed by flow cytometry (FCM) using the same procedures, reagents and cytometer employed by Yokoya et al. (2000). Petroselinum crispum “Champion Moss Curled” (2n=2x=22; 2C DNA amount=4.46 pg) was used as an internal calibration standard, and 4′,6-diamidino-2-phenylindole (DAPI) was used as the fluorochrome (Yokoya et al. 2000). Nuclei of anise hyssop leaves were mostly at the G1 (pre-replicative) stage of cell division, and only few of them were at the G2 (post-replicative) stage so that mixoploids could be identified in the FCM histograms by the presence of two large peaks. Size and density of stomata For this purpose, six diploid plants (control) and six plants of tetraploid plants were randomly selected. Measurement and scoring were performed for four well-expanded leaves of each plant. Three samples of epidermal cells were obtained from lower surface by nail varnish technique. A small area of the abaxial side of leaves was covered with a thin layer of clear nail polish and left to dry. Then, it was removed with a pair of fine tip forceps. The polish strips were mounted on a micro- scope slide and then evaluated for the density and size of leaf stomata under the light microscope (Olympus BX40
Imagine tetraploid male strain...
Naturally occuring what are the odds?
Something is producing natural triploid sterile females...
Seed treatment In the second experiment, different concentrations of colchicine, oryzalin and trifluralin were used to treat the seeds by soaking them in the antimitotic solutions for 6, 12 or 24 h. In each treatment, 50 seeds were used, and Tween “20” was added as surfactant for better efficiency of the treatments. The treated seeds were washed with distilled water and planted in the trays filled with peat moss
erlite:soil (1:1:1) in greenhouse under 16 h light period, 27–24°C day/night temperature and 63% humid- ity. This experiment was arranged in a factorial based on a completely randomized design with five replications. Seedling treatment In the third experiment, the seeds were planted in plant- ing trays. At the emergence of two true leaves stage, the seedlings were immerged in the above mentioned con- centrations of colchicine, oryzalin and trifluralin for 6, 12 or 24 h. In each treatment, 50 seedlings were used. The treated seedlings were washed with distilled water and carefully planted in the pots filled with a mixture of leaf mold:sand:loam soil (1:1:1). The pots were put in greenhouse with 16 h light period, 27–24°C day/night temperature and 63% humidity. This experiment was a factorial arrangement based on a completely rand- omized design with five replications. Flow cytometry Ploidy was assessed by flow cytometry (FCM) using the same procedures, reagents and cytometer employed by Yokoya et al. (2000). Petroselinum crispum “Champion Moss Curled” (2n=2x=22; 2C DNA amount=4.46 pg) was used as an internal calibration standard, and 4′,6-diamidino-2-phenylindole (DAPI) was used as the fluorochrome (Yokoya et al. 2000). Nuclei of anise hyssop leaves were mostly at the G1 (pre-replicative) stage of cell division, and only few of them were at the G2 (post-replicative) stage so that mixoploids could be identified in the FCM histograms by the presence of two large peaks. Size and density of stomata For this purpose, six diploid plants (control) and six plants of tetraploid plants were randomly selected. Measurement and scoring were performed for four well-expanded leaves of each plant. Three samples of epidermal cells were obtained from lower surface by nail varnish technique. A small area of the abaxial side of leaves was covered with a thin layer of clear nail polish and left to dry. Then, it was removed with a pair of fine tip forceps. The polish strips were mounted on a micro- scope slide and then evaluated for the density and size of leaf stomata under the light microscope (Olympus BX40
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heirloomganja
Active member
acespicoli
Well-known member
H9 PK ZC (Nh)
edited thx @pipeline
Front. Plant Sci., 20 December 2018
Sec. Crop and Product Physiology
Volume 9 - 2018 | https://doi.org/10.3389/fpls.2018.01876
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acespicoli
Well-known member
acespicoli
Well-known member
Good catch my friend I meant to add the citation and had a distraction
Front. Plant Sci., 20 December 2018
Sec. Crop and Product Physiology
Volume 9 - 2018 | https://doi.org/10.3389/fpls.2018.01876
Ace has one of the most diverse Yunnan hybrids in the catalog they say its kinda complex in aroma ???
Description copy pasted below
Looks like it throws phenos from sativa to indica and in between.... what id expect in a landrace
China Yunnan Regular - aceseeds
EXOTIC AND RESINOUS ASIAN VARIETY WITH PENETRATING AROMAS AND PLEASANT EFFECTS
www.aceseeds.org
if you try this feedback is appreciated its on the list of todo's
ACE Seeds' China Yunnan Description
Landrace / Inbred Line (20% Sativa / 80% Indica)
This exotic plant, a possible connection between the classical indicas and the South Asian sativas, shows features of both sides of the cannabis. On one hand, it grows strong and compact with beautiful wide leaves and a hash plant look. Its flower ripens quickly with a high resin production and a creamy scent.On the other hand, it shows some Asian sativa features which can be appreciated in the beginning of its effect and in certain incensed and woody aromas. With all our fondness, we offer to the growers’ community this rarity, hardly introduced in the modern breed. We hope you preserve it and use it for your breeding projects. Excellent adapted to indoor grows. For outdoor grows, warm and dry climates are recommended. Medium resistance against spider mite, oidium and Botrytis. Low resistance against cold.
Structure: Robust, short-knoted. Indoors, with short veg cycles, it produces columnar plants with a big main bud. Outdoors, with longer veg cycles, it develops a branched, ball-shaped structure.
Bouquet: Dense, sweet and creamy indica aroma, with woody and spicy Asian sativa touches.
High: Happy and visual at first, then warm, relaxing and pleasant in the end.
Genetics: Dominant indica line coming from Yunnan, a southern China province which borders on Birmania/Myanmar.
Flowering indoors: 8-9 weeks.
Flowering outdoors: End of September / Early October.
Yield per m²: Medium-High
China Yunnan (ACE Seeds) :: Cannabis Strain Gallery
All ACE Seeds China Yunnan plant pictures in the SeedFinder cannabis strain gallery.
- China Yunnan »»» China Yunnan IBL
- China Yunnan »»» Mostly Indica
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acespicoli
Well-known member
Xmas is a cross made by subcool and ndn79. The Christmas used that they made has Gainesville Green, mycanopie and moonbeam. Gainesville green was a term used to describe kind cannabis in Florida like the name OG kush in California. UM took a male and female from this progeny and crossed it to the f2 duckfoot. Out of 6 seeds one showed a full web mutation that turned male the others shown recessive boxing glove web mutations. These were crossed and made the web mutated X-Masduck chemovar. It smells of creamy xmas ribbon candy with a dank skunk aroma. The taste is creamy with hints of pepper and anise. Used to help inflammation headaches, body aches, to helping you sleep at night. This had spina bifida, epilepsy and A.D.D. patients in mind to help calm the brain.
XmasDuck Lineage / Genealogy
acespicoli
Well-known member
What do we know about the unknown/legendary Ducksfoot?
Ducksfoot is named for its unique leaves, which have webbing between the leaflets, evoking the feet of waterfowl, rather than the fingerlike morphology of the elaves that has become one of the most recognizable icons to identify pot.In Australia, Ducksfoot is an outdoor variety only. This strain seems more at home in warmer climates that don’t suffer frosts too early. Several outdoor grows in temperate regions of the Northern hemisphere showed good growth but the quantity and the quality of the buds were poor when compared with its native climate of Australia.
Ducksfoot Hybrids / Crossings Selections / Direct Descendents Agosto Pato Duck » Ducksfoot x Ducksfoot Ducksfoot » Ducksfoot x Ducksfoot F5 Ducksfoot » Ducksfoot
Northern Lights #2 »»» Northern Lights #2
Murphy Stevens x ndn79
dogzter
Drapetomaniac
I gave the 79xmas seeds to ndnguy.
Subcool didn't have anything to do with them either.
acespicoli
Well-known member
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acespicoli
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The first time I smoked those wasnt till 88' - 90' pretty sure it was the same line
Lots of brass looking hair and smelled of pine just like a fresh cut xmas tree
Was thinking about picking some up to reminisce.
The old strain I got was stony laid lots of people down for a nap
Imagine it would be good for med patient pain?
Wont ask too many questions
Just that one
acespicoli
Well-known member
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heirloomganja
Active member
Seed catalog from Reeferman around 2005.
'79 Xmas Bud IBL - Humboldt CSI
1979 Xmas Bud IBL Open pollinated reproduction of the pure […]
humboldtcsi.com
Got this as well as some others as freebies when I placed a couple orders last year. I messed up and let them get too cool as young seedlings, so they got scrapped for the outdoor project last year. Not sure if I have any left. I need to look!
I love origin of species studies. Really interesting stuff. Thats my type of history. Thanks!
acespicoli
Well-known member
Cannabinoid Inheritance Relies on Complex Genetic Architecture - PMC
Introduction: Understanding the inheritance of cannabinoid compounds in Cannabis sativa will facilitate effective crop breeding and careful regulation of controlled substances. The production of two key cannabinoids, Δ-9-tetrahydrocannabinol (THC) ...
www.ncbi.nlm.nih.gov
Results: Unlike previous research, we note nonadditive components of cannabinoid inheritance. Concentration of THC is a polygenic trait (three to four genetic factors). Both additive and dominance CGEs best explained THC expression patterns. In contrast, cytoplasmic genomes and additive genes may influence CBD concentration. Maternal additive effects and additive genetic effects apparently influence CBC expression.
