Type of explant | Genotype (Type) | Number of shoots obtained | References |
---|---|---|---|
Nodal segments | MX-1 (drug) | 13.8 shoots/explant | |
Shoot tips | Changtu (fiber) | 3.22 shoots/shoot tip | |
Nodal segments | Mexican variety (drug, THC rich) | 13.44 shoots/explant | |
Immature and mature floral explants | 1KG2TF (drug, THC rich) | 4a shoots/immature inflorescence | |
S1525 (drug, THC rich) | < 1a shoot/immature inflorescence | ||
Isolated meristem | USO-31 (fiber, < 3% CBD) | 4.4 shoots/explant | |
Shoot apex | 3.2 shoots/explant | ||
Cotyledonary node | 2.4 shoots/explant | ||
Nodal segments | High CBD variety | 3.63 shoots/explant | |
High CBG variety | 3.38 shoots/explant | ||
Nodal segments | MX-CBD-11 (drug, CBD rich) | 3.00 shoots/explant | |
MX-CBD-707 (drug, CBD rich) | 2.43 shoots/explant | ||
Shoot tips of epicotyl | Diana | 3.4 shoots/explant | |
Fedora 17 | 3.2 shoots/explant | ||
Finola (all hemp) | 3.7 shoots/explant | ||
2 cm stem tips | Wife (hemp) | 8.4 micro-cuttings/4 explants | |
Meristems | Pure CBD | 1.9 shoots/explant | |
Nodal segments | Cheesequake | 0.5 shoots/explant | |
| Moby Dick | 1.9 shoots/explant | |
| Space Queen | 0.2 shoots/explant | |
| Pennywise (all drug type) | 2.0 shoots/explant | |
| BLD | 0.4 shoots/explant | |
| SWD | 0.4 shoots/explant | |
| Moby Dick (all drug type) | 0.3 shoots/explant | |
Single floret | U82 (drug, THC rich) | 18.2 shoots/explant | |
Pair of florets | 14.7 shoots/explant | ||
Shoot tip | US Nursery Cherry I (drug) | 5.75 shoots/explant, over 4 cycles | |
Two-node explants | BA-21 (drug, code name) | 2.23 shoots/explant | |
BA-41 (drug, code name) | |||
BA-49 (drug, code name) | |||
BA-61 (drug, code name) | |||
BA-71 (drug, code name) | |||
Tip cuttings | Epsilon 68 (fiber, CBD rich) | 2.5 shoots/explant | |
Nodal cuttings | 1.3 shoot/explant | ||
Secondary tip and nodal cuttings | 3 shoots/explant | ||
Microshoots | Abacus (hemp) | 2.6 shoots/explant | |
Wife (hemp) | 3.7 shoots/explant | ||
Stem segments with two nodes | Honey Banana (THC-rich) | 1.5 shoot/explant | |
1-cm single-node stem segment | TJ’s CBD (CBD-rich) | >20a shoots/explant (TDZ, low quality) | |
8a shoots/explant (BA, high quality) | |||
Shoot segments with one node | Safari Punch (THC-dominant) | 5.33 shoots/explant | |
Peanut Sundae (THC-dominant) | 2a shoots/explant | ||
Master Hemp (CBD-dominant) | 5.4 shoots/explant | ||
Orange Mimosa Purple (THC rich) | 2.4a shoots/explant | ||
Golden Papaya (THC-dominant) | 3.33 shoots/explant |
Type of explant | Genotype | Type | Callus stage | Best outcome | References |
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