:D Genetic Preservation :D - Breeding

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'2006 HT

 

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https://www.researchgate.net/figure/Percentage-of-recurrent-parent-genome-after-backcrossing_tbl3_226925645

 

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​

BC's best Strain. This variety is a mostly indica heavyweight plant that grows and finishes fast. It's easy to grow and versatile. You'll love the extremely resinous sticky buds and the strong cerebral high. Obviously an excellent hash plant. An absolute must for beginners or experts alike. Highly renowned throughout British Columbia and the world for its fast finishing times, abundant resin production and pleasant stone everytime.

THC: 18-25%

• BC HashPlant »»» BC Hash Plant
• BC Hash Plant IBL
  • Hash Plant
    • USA, Kalifornien »»» Indica

The Seed Banks Hash Plant Description​

May 1, 1987
Our line of Hash Plant seeds originated with a cutting of the original Hash Plant clone, one of the top indoor varieties from the Northwest USA. The seeds from this clone, fertilized with NL#1, resulted in the strongest, most resinous plants that we have ever grown. The quality of the finished bud was astonishing; these are undoubtedly the most potent plants in the world at the moment. The buds finished beautifully, even under lower light levels, and they were quick, finishing in 50 +/- days of flowering. Every seedling was excellent, and 1 out of 4 was truly outstanding. The seeds now available are the result of an backcross of a male from this generation back to the original clone. This should therefore result in 50% outstanding Hash Plant types.
An absolute must for the connoisseur,

but please handle with care, inexperienced smokers may have problems.

• Hash Plant »»» Hash Plant x {Hash Plant x NL #1}

 

[Old Piney]

View attachment 18894783
Good news ApeOrigin has done a reproduction of blue hemps thai
They have them over at cannapot atm as well as some other classic by the same

ApeOrigins Thaifun Horizon Description​

Thaifun Horizon is one of the legendary outdoor-strains that was created by Bluehemp Genetics in Switzerland at the early century, this one is pure Thai of best quality. But this landrace is a very special one that was attapted to Suisse area, it is a pure one but it is much faster as other sativas from Thailand. The flavour is strong but not too strong, it reminds on sweet n sour thaigras from the 70s, absolutely delicious and mouthwatering - but beware, even if the smell is not very strong, this pure sativa offers a massive power that is not easy to handle, you will think you are on a magic carpet ride.

This ...

ApeOrigin is the union of ApeGenetics and Originseeds - a new breedercollective located all over the world with the target to reproduce medicinal, old, legendary and rare strains and landraces as well. Every breeder has his own individual target - creating strains for outdoor- or indoorgrowers. The collective tries to offer more and more rare strains in future - especially landraces which are not available anywhere else.

ApeOrigin :: Breeder Info & Cannabis Strains

ApeOrigin is the union of ApeGenetics and Originseeds - a new breedercollective located all over the world with the target to reproduce medicinal, old, legen...

en.seedfinder.eu

Cannapot Onlineshop SLU - Thaifun Horizon (Bluehemp repro)

Thaifun Horizon - Ape OriginThaifun Horizon is one of the legendary outdoor-strains that was created by Bluehemp Genetics in Switzerland at the early century, this one is pure Thai of best quality. But this landrace is a very special one that was attapted to Suisse area, it is a pure one but it...

www.cannapot.com

and if you get some holdme down on the f2
got to much thai weed going on right now but it would be nice to hold on to for summer
Really a struggle not to buy these up....hopefully someone does and shares them around
Does the growth pattern look sativa thai ?
10/ $28 its like a sore cant beat it

ApeOrigin - rare cannabisseeds, cheap but with real high quality

A Breedercollective with the target to reproduce medicinal, old, legendary and rare strains and landraces as well - creating strains for outdoor- and indoorgrowers.

www.cannapot.com

their bushmans and g13 look like winners as well

Just received these in the mail today will definitely be giving it a go outside this season

 

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Just received these in the mail today will definitely be giving it a go outside this season View attachment 19011802

Have a few old ones glad you posted reminds me to grab some fresh stock while its available, looking foward to seeing these outdoors!

Best

 

[mudballs]

A true landrace isn't heterozygous it's homozygous...after 10yrs left alone it is well past F6. From what i looked at you can preserve with around 250 plants, BUT, you have to leave any hermaphrodites in the population ..yeah...those matter for true genetic diversity in the group.

 

[pipeline]

Cannapot Onlineshop SLU - Blueberry

The DJ Short Blueberry is a mostly indica (bout 80% indica and 20% sativa) that comes from the late 70s. This is the original and great Blueberry with the great smell and the touch of oldschool many gardeners are searching for. A great winnerstrain!!!

www.cannapot.com

 

[acespicoli]

I read somewhere that DJ Short and Dutch Passion had a distribution agreement and now DP is in te USA direct distributing genetics. The Blue Indica is kinda calling I grew flo it was nice. Someone else has the Colorado Blueberry cut and used it for a release https://www.greenmanorganics.net/products email him

https://dcseedexchange.com/product-category/dj-short-old-world-genetics/

Cannapot is a solid source these were my wish list been awhile since I had some DJ stuff

• 
  
  Dj Short's Old World Genetics
  

  The Original Blueberry F5 6 Regular Seeds​

• 
  Dj Short's Old World Genetics
  

  The Original Flo F5 6 Regular Seeds​

  
  
  
  
  
  
  
  OUT OF STOCK
  Dj Short's Old World Genetics
  

  DJ Short’s Breeders’ Choice Mix # 4 16 Regular Seeds​

 

[acespicoli]

The Inheritance of Chemical Phenotype in Cannabis sativa L.

Etienne P. M. de Meijer,*,1 Manuela Bagatta,† Andrea Carboni,† Paola Crucitti,† V. M. Cristiana Moliterni,† Paolo Ranalli† and Giuseppe Mandolino†,2 *HortaPharm B.V., 1075 VS, Amsterdam,

The Netherlands and † Istituto Sperimentale per le Colture Industriali, 40128 Bologna, Italy Manuscript received April 23, 2002 Accepted for publication October 16, 2002

ABSTRACT Four crosses were made between inbred Cannabis sativa plants with pure cannabidiol (CBD) and pure -9-tetrahydrocannabinol (THC) chemotypes. All the plants belonging to the F1’s were analyzed by gas chromatography for cannabinoid composition and constantly found to have a mixed CBD-THC chemotype. Ten individual F1 plants were self-fertilized, and 10 inbred F2 offspring were collected and analyzed. In all cases, a segregation of the three chemotypes (pure CBD, mixed CBD-THC, and pure THC) fitting a 1:2:1 proportion was observed. The CBD/THC ratio was found to be significantly progeny specific and transmitted from each F1 to the F2’s derived from it. A model involving one locus, B, with two alleles, BD and BT, is proposed, with the two alleles being codominant. The mixed chemotypes are interpreted as due to the genotype BD/BT at the B locus, while the pure-chemotype plants are due to homozygosity at the B locus (either BD/BD or BT/BT). It is suggested that such codominance is due to the codification by the two alleles for different isoforms of the same synthase, having different specificity for the conversion of the common precursor cannabigerol into CBD or THC, respectively. The F2 segregating groups were used in a bulk segregant analysis of the pooled DNAs for screening RAPD primers; three chemotypeassociated markers are described, one of which has been transformed in a sequence-characterized amplified region (SCAR) marker and shows tight linkage to the chemotype and codominance.

DISCUSSION Within Cannabis populations, large variations in cannabinoid composition and content can be found among individual plants. Therefore, to study the inheritance of the chemotype trait, we chose to use inbred, female lines with fixed, pure chemotype. RAPD data confirmed the relative narrowing of the genetic basis of the parental plants due to this strategy (Table 1). In the parental S2 lines, the percentage of the major cannabinoid (PCn) ranged from 84 to 98% of the total cannabinoid fraction Ctot. For the F1 99.3, 99.5, and 99.6 and for their F2 offspring, the sum of CBD and THC reached proportions of 95% of Ctot. The remaining fractions were composed of varying mixtures of CBG, CBC, THCV, and CBDV. The 99.4 F1’s and their F2 descendants had a lower proportion of CBD THC (91%). This was due to a consistent presence of higher amounts of CBG, a feature inherited from both parents of this progeny. The uniformity of F1 chemotypes and the F2 segregation ratios demonstrate the presence of a single locus, which is referred to as B, showing simple Mendelian inheritance of the two alleles, BD and BT, evidenced by this study. The model proposes that a pure CBD plant has a BD/BD genotype at the B locus, while a pure THC plant has a BT/BT genotype. F1 and 50% of the F2 plants are therefore heterozygous BD/BT, with the two alleles being codominant and therefore simultaneously expressed in the hybrids. The hypothesis of two alleles at one locus was accepted by 2 tests for all the F2

 

[mudballs]

Damn...we lost @dx4 didn't we?
"It is suggested that such codominance is due to the codification by the two alleles for different isoforms of the same synthase"
He knows this stuff..

 

[pipeline]

Yeah thats a pretty old study. Wonder how the inheritance is for other cannabinoids and terpene profiles? Terpenes and flavinoids and ketones probably get complicated.

Seems like a pretty straight forward inheritance for THC and CBD. Makes it convenient how they segregate for breeding.

 

[mudballs]

...@dx4 knows...

 

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All these locations matter...not just CBD/THC chemovar...if we want to trully control the plant

 

[mudballs]

And the isoform of the synthase? ..yeah im out of my depth now..

 

[pipeline]

isoform , the synthase is what reads the DNA to create the protein

It is suggested that such codominance is due to the codification by the two alleles for different isoforms of the same synthase, having different specificity for the conversion of the common precursor cannabigerol into CBD or THC, respectively.

Isoform is the type of enzyme synthase that converts cannabigerol. Allele is a form of a gene.

 

[mudballs]

isoform , the synthase is what reads the DNA to create the protein

Isoform is the type of enzyme synthase that converts cannabigerol. Allele is a form of a gene.

I think I've contributed in our game, i can pass on this exam and let another colleague pick up the interest

 

[acespicoli]

Gene isoform - Wikipedia

en.wikipedia.org

Locus (genetics) - Wikipedia

en.wikipedia.org

Allele - Wikipedia

en.wikipedia.org

Yeah thats a pretty old study. Wonder how the inheritance is for other cannabinoids and terpene profiles? Terpenes and flavinoids and ketones probably get complicated.

Seems like a pretty straight forward inheritance for THC and CBD. Makes it convenient how they segregate for breeding.

The biosynthesis of the cannabinoids - PMC

Cannabis has been integral to Eurasian civilization for millennia, but a century of prohibition has limited investigation. With spreading legalization, science is pivoting to study the pharmacopeia of the cannabinoids, and a thorough understanding ...

www.ncbi.nlm.nih.gov

Frontiers | Cannabinoids and Terpenes: How Production of Photo-Protectants Can Be Manipulated to Enhance Cannabis sativa L. Phytochemistry

Cannabis sativa L. is cultivated for its secondary metabolites, of which the cannabinoids have documented health benefits and growing pharmaceutical potentia...

www.frontiersin.org

I liked what @dubi said in one of his posts about the longer flowering sativa effects were preferred
In the biosynthesis some of the terpenes and cannabinoids are very complex and long chain then you think
How long do these chemical reactions take to complete in days weeks months, in varying conditions

THCV ? what others ... cannabis science is in its infancy just scratching the surface
now that its not so taboo


https://en.wikipedia.org/wiki/Polyketide (good read)
Polyketides can be produced in bacteria, fungi, plants, and certain marine organisms.[6]

Applications​

Polyketide antibiotics,[22] antifungals,[23] cytostatics,[24] anticholesteremic,[25] antiparasitics,[23] coccidiostats, animal growth promoters and natural insecticides[26] are in commercial use.

Medicinal​

There are more than 10,000 known polyketides, 1% of which are known to have potential for drug activity.[27] Polyketides comprise 20% of the top-selling pharmaceuticals with combined worldwide revenues of over USD 18 billion per year.[28]
TOOK A FEW MINUTES TO SUMMARIZE IT FOR EVERYONE

Sesquiterpene - Wikipedia

en.wikipedia.org

(im working these now)

Geranyl pyrophosphate - Wikipedia

en.wikipedia.org

A recent study conducted in the Cosmics Leaving Outdoor Droplets large cloud chamber at CERN, has identified sesquiterpenes—gaseous hydrocarbons that are released by plants—as potentially playing a major role in cloud formation in relatively pristine regions of the atmosphere.[2]

alot of biosynthesis potential in the garden,
One pill makes you larger, and one pill makes you small

CBD THC

Traditional Chinese Medicine

The exhibit Traditional Chinese Medicine compiles early influences, ancient texts, and public health posters to illustrate Chinese public health campaigns.

www.nlm.nih.gov

 

[mudballs]

We're just now getting to work with these concepts, 2022 study

Cannabidiol, ∆9-tetrahydrocannabinol, and metabolites in human blood by volumetric absorptive microsampling and LC-MS/MS following controlled administration in epilepsy patients

Cannabidiol (CBD) exhibits anti-inflammatory, anxiolytic, antiseizure, and neuroprotective proprieties without addictive or psychotropic side effects, as opposed to Δ[9] -tetrahydrocannabinol (THC). While recreational cannabis contains higher ...

www.ncbi.nlm.nih.gov

Epilepsy kids treated with CBD for blood testing...so now, IF we identify a cannabinoid profile that helps more, we gotta know what loci alleles or gene group combination creates a plant to produce more of that cannabinoid profile.."ok we have CBD pheno here and not a THC"...good for you, does it have the right chromosomal make up to produce more 6-alpha-hydroxy-cannabidiol?
"Fk if i know. How do we do that"

 

[acespicoli]

We're just now getting to work with these concepts, 2022 study

Cannabidiol, ∆9-tetrahydrocannabinol, and metabolites in human blood by volumetric absorptive microsampling and LC-MS/MS following controlled administration in epilepsy patients

Cannabidiol (CBD) exhibits anti-inflammatory, anxiolytic, antiseizure, and neuroprotective proprieties without addictive or psychotropic side effects, as opposed to Δ[9] -tetrahydrocannabinol (THC). While recreational cannabis contains higher ...

www.ncbi.nlm.nih.gov

Epilepsy kids treated with CBD for blood testing...so now, IF we identify a cannabinoid profile that helps more, we gotta know what loci alleles or gene group combination creates a plant to produce more of that cannabinoid profile.."ok we have CBD pheno here and not a THC"...good for you, does it have the right chromosomal make up to produce more 6-alpha-hydroxy-cannabidiol?
"Fk if i know. How do we do that"

Right?

Medical Strain Finder :: Medicinal marijuana varieties

Here the SeedFinder gathers and shares information on individual medical cannabis varieties.

en.seedfinder.eu

Leafly offers a good cloud selection helpful in finding those strains
Not having advanced DNA resources, I collect plant lab results for thc cbd and terpenes

Best Healthiest non smoke form? Hash Oil, RSO, Honey, Kif,etc - Treating pain and cancer

https://cannabisdatabase.ca/unearth/q?utf8=%E2%9C%93&query=CANCER&searcher=compounds&button= https://drive.google.com/file/d/1Ub4MS9m2e6NuBmm7gXAVvn9II-e5JdFQ/view?usp=sharing As promised edits... an index to the thread as its posted by users key sections last update 7.25.2023 Most important...

www.icmag.com

Collect alot of that type data here ^

Best