acespicoli
Well-known member
NICE collection they look well stored reminds me of grandmas cookie jar
be hard to pick, which one of those to try
Skinny Leaf
If you have a moment and see this please give me a explanation of the filling and sealing of the Ampoules you use for seed storage.
As well as the opening procedure you use.
Those containers are the best example of a long term storage solution in my opinion it took me awhile to realize they are lab grade cryo storage containers
Found a case of 1ml Ampoules 144/ 35$ USD on Amazon and might give them a go was thinking about a 19.99 opener and the glass rod to seal them
What size exactly are you using and do you find that even large cannabis seeds will fit ?
Q- 'Hi (chimera). I was wondering if you can tell me what is the best way to freeze pollen in?
what should the pollen be put in ive read a few different ways but i thought you would know best."
A- Flower pollen donors. Cut the stalks of large branches at a 45 degree angle just before the flowers begin to open. Place in a vase of fresh water, stem down, as you would with cut flowers.
When the staminate flowers begin to point downwards, remove all but the floral material (ie leaves etc) with sharp scissors... be gentle as the more you knock the flowers around, the more pollen you'll lose.
When the pollen starts to drop, place the branches on a sheet of aluminum foil or a dry piece of paper in a room with no draft or air currents. Leave for 12 hours and shake very gently, flip them over on their backs and repeat the process..
Collect the pollen, remove debris (plant matter, flowers etc), screen it with a 40u screen or the like, and dry it for a day in a low humidity room on a piece of printer paper laid over some cardboard stock- this will help dry out the pollen.
Collect the pollen and freeze it in an airtight container like an old film canister or a small tupperware container. Dip the top of the container in liquid wax if you think the seal is not airtight. Freeze in a stable temperature freezer, -20 C or deeper is best.
Dividing the pollen into multiple 'satchels' or small envelopes allows you to break into your pollen collection and pull some out a little bit at a time without contaminating the whole batch. Work quickly when the pollen is pulled out of the freezer! Put the remaining 'packets' back in the freezer ASAP.
Remember, a little goes a long way so don't put it all in a single envelope.
Pollen stored using these principles has allowed breeders to store pollen for up to 17 years and still remain viable. I suspect it would last longer still, but these are the results we currently have to work with.
Simple as.
You can use the old paint brush method to apply the pollen, but Mel Frank recently showed me that using a clean finger dipped into the pollen can be more effective and give more control over the pollen, since the natural oils on our skin act as a 'sticker' and keeps the pollen from flying around where it might not be wanted. Just gently rub your fingers along the stigmatic surface of the pistils, and you should get a pretty decent seed set. Certainly enough for home crosses, preservation, or test crosses.
Enjoy!
-Chimera
Also would like to say this is a rad fucking thread! Way to be acespicoli, those ampoules are so cool. First time I have ever seen anything like that, thanks for the introduction. Hope your day or night is as awesome as you!
indicates that these average 2.2 mm in length (dime is 18 mm, and Chinese culinary seeds are 7.2 mm