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CANNABIS REFERENCES for breeding
- Thread starter Sam_Skunkman
- Start date
The inheritance of chemical phenotype in Cannabis sativa L. (IV): cannabinoid-free plants
E. P. M. de Meijer, K. M. Hammond and A. Sutton
[highlight]Euphytica 168, 95 (2009)[/highlight]
The inheritance of chemical phenotype in Cannabis sativa L. (III): variation in cannabichromene proportion
E. P. M. de Meijer, K. M. Hammond and M. Micheler
[highlight]Euphytica 165, 293 (2009)[/highlight]
E. P. M. de Meijer, K. M. Hammond and A. Sutton
[highlight]Euphytica 168, 95 (2009)[/highlight]
The inheritance of chemical phenotype in Cannabis sativa L. (III): variation in cannabichromene proportion
E. P. M. de Meijer, K. M. Hammond and M. Micheler
[highlight]Euphytica 165, 293 (2009)[/highlight]
just read through this, thanks heaps sam skunkman! bump 
M
madback
wow.. theres some great reading in those links! thanks!!!
love?
Member
Not a paper about breeding per se but I thought some might find it interesting or maybe useful as a scientific reference for stuff that's common knowledge in the growing world but maybe not outside it.
The results of an experimental indoor hydroponic Cannabis growing study, using the ‘Screen of Green’ (ScrOG) method—Yield, tetrahydrocannabinol (THC) and DNA analysis
Glenys Knight, Sean Hansen, Mark Connor, Helen Poulsen, Catherine McGovern and Janet Stacey
http://linkinghub.elsevier.com/retrieve/pii/S0379-0738(10)00196-9
Abstract
The results of an indoor hydroponic Cannabis growth study are presented. It is intended that this work will be of assistance to those with an interest in determining an estimation of yield and value of Cannabis crops. Three cycles of six plants were grown over a period of 1 year in order to ascertain the potential yield of female flowering head material from such an operation. The cultivation methods used were selected to replicate typical indoor hydroponic Cannabis growing operations, such as are commonly encountered by the New Zealand Police. The plants were also tested to ascertain the percentage of the psychoactive chemical Delta-9 tetrahydrocannabinol (THC) present in the flowering head material, and were genetically profiled by STR analysis. Phenotypic observations are related to the data collected. The inexperience of the growers was evidenced by different problems encountered in each of the three cycles, each of which would be expected to negatively impact the yield and THC data obtained. These data are therefore considered to be conservative. The most successful cycle yielded an average of 881g (31.1oz) of dry, groomed female flowering head per plant, and over the whole study the 18 plants yielded a total of 12,360g (436.0oz), or an average of 687g (24.2oz) of dry head per plant. THC data shows significant intra-plant variation and also demonstrates inter-varietal variation. THC values for individual plants ranged from 4.3 to 25.2%. The findings of this study and a separate ESR research project illustrate that the potency of Cannabis grown in New Zealand has dramatically increased in recent years. DNA analysis distinguished distinct groups in general agreement with the phenotypic variation observed. One plant however, exhibiting a unique triallelic pattern at two of the five loci tested, while remaining phenotypically indistinguishable from three other plants within the same grow.
Conclusion
The aim of this study was to ascertain the potential yield of Cannabis female flowering head which could be obtained from crops of six plants grown in a given area using a particular growing method (ScrOG). This information is needed by the New Zealand Courts when they are considering the severity of the offending, and in particular, how much income is being derived from such an illegal operation.
As an adjunct to the study, we made phenotypic observations, analysed the THC levels from the plants grown and undertook DNA analysis in order to see whether there was any genetic basis to the chemical and phenotypic differences observed.
Of the 18 plants grown, the yield varied between a maximum of 47.27 oz and 11.99 oz of dry, groomed female flowering head per plant, with an average of just over 1.5 lb of head per plant. This demonstrates that it is possible to obtain at least 47.27 oz from a single plant. However, it has not been possible to show whether greater yields are possible, or to determine the mean or variance to be expected from such a method. This is mainly for two reasons: (1) the problems encountered during the growing, and (2) the small number of plants grown in this study.
This study has demonstrated that it is possible to obtain THC levels up to a maximum of 30% from Cannabis plants available in New Zealand. However, further research is needed to ascertain what the mean and variance of THC levels in New Zealand Cannabis populations may be, and the work of Hassan and Sommerville, referred to in Section 3.4, is intending to address this question.
DNA analysis distinguished distinct groups of plants in general agreement with the phenotypic variation observed, with the exception of one plant of the indica-like group from Grow 1. For example, DNA analysis determined that the two plants that exhibited the characteristics of the subspecies sativa were closely related. These two plants produced significantly higher THC levels than the four plants with the physical characteristics of the subspecies indica. As all other environmental and nutritional parameters affecting the six plants were controlled, this suggests that plant variety influences THC levels. A much wider study would be required however, to ascertain whether varieties within these two subspecies consistently produce THC levels within distinct ranges, or whether there is also considerable variation in THC levels within these subspecies.
The results of an experimental indoor hydroponic Cannabis growing study, using the ‘Screen of Green’ (ScrOG) method—Yield, tetrahydrocannabinol (THC) and DNA analysis
Glenys Knight, Sean Hansen, Mark Connor, Helen Poulsen, Catherine McGovern and Janet Stacey
http://linkinghub.elsevier.com/retrieve/pii/S0379-0738(10)00196-9
Abstract
The results of an indoor hydroponic Cannabis growth study are presented. It is intended that this work will be of assistance to those with an interest in determining an estimation of yield and value of Cannabis crops. Three cycles of six plants were grown over a period of 1 year in order to ascertain the potential yield of female flowering head material from such an operation. The cultivation methods used were selected to replicate typical indoor hydroponic Cannabis growing operations, such as are commonly encountered by the New Zealand Police. The plants were also tested to ascertain the percentage of the psychoactive chemical Delta-9 tetrahydrocannabinol (THC) present in the flowering head material, and were genetically profiled by STR analysis. Phenotypic observations are related to the data collected. The inexperience of the growers was evidenced by different problems encountered in each of the three cycles, each of which would be expected to negatively impact the yield and THC data obtained. These data are therefore considered to be conservative. The most successful cycle yielded an average of 881g (31.1oz) of dry, groomed female flowering head per plant, and over the whole study the 18 plants yielded a total of 12,360g (436.0oz), or an average of 687g (24.2oz) of dry head per plant. THC data shows significant intra-plant variation and also demonstrates inter-varietal variation. THC values for individual plants ranged from 4.3 to 25.2%. The findings of this study and a separate ESR research project illustrate that the potency of Cannabis grown in New Zealand has dramatically increased in recent years. DNA analysis distinguished distinct groups in general agreement with the phenotypic variation observed. One plant however, exhibiting a unique triallelic pattern at two of the five loci tested, while remaining phenotypically indistinguishable from three other plants within the same grow.
Conclusion
The aim of this study was to ascertain the potential yield of Cannabis female flowering head which could be obtained from crops of six plants grown in a given area using a particular growing method (ScrOG). This information is needed by the New Zealand Courts when they are considering the severity of the offending, and in particular, how much income is being derived from such an illegal operation.
As an adjunct to the study, we made phenotypic observations, analysed the THC levels from the plants grown and undertook DNA analysis in order to see whether there was any genetic basis to the chemical and phenotypic differences observed.
Of the 18 plants grown, the yield varied between a maximum of 47.27 oz and 11.99 oz of dry, groomed female flowering head per plant, with an average of just over 1.5 lb of head per plant. This demonstrates that it is possible to obtain at least 47.27 oz from a single plant. However, it has not been possible to show whether greater yields are possible, or to determine the mean or variance to be expected from such a method. This is mainly for two reasons: (1) the problems encountered during the growing, and (2) the small number of plants grown in this study.
This study has demonstrated that it is possible to obtain THC levels up to a maximum of 30% from Cannabis plants available in New Zealand. However, further research is needed to ascertain what the mean and variance of THC levels in New Zealand Cannabis populations may be, and the work of Hassan and Sommerville, referred to in Section 3.4, is intending to address this question.
DNA analysis distinguished distinct groups of plants in general agreement with the phenotypic variation observed, with the exception of one plant of the indica-like group from Grow 1. For example, DNA analysis determined that the two plants that exhibited the characteristics of the subspecies sativa were closely related. These two plants produced significantly higher THC levels than the four plants with the physical characteristics of the subspecies indica. As all other environmental and nutritional parameters affecting the six plants were controlled, this suggests that plant variety influences THC levels. A much wider study would be required however, to ascertain whether varieties within these two subspecies consistently produce THC levels within distinct ranges, or whether there is also considerable variation in THC levels within these subspecies.
that's great love? i just downloaded it! cheers mate
Attached.
takkada
Member
thanks for some freebies, i got skunk #1 x haze germinating now. they sound awsome. thanks. your breeding info is very helpfull.
Cannabis - from cultivar to chemovar
A. Hazekamp and J. T. Fischedick
Drug Test. Analysis 2012, 4, 660–667
A. Hazekamp and J. T. Fischedick
Drug Test. Analysis 2012, 4, 660–667
Hi, just wanna post a mystical reference diametrically opposite to help all natural scientists people to better understand the workings of love, nature and thus cannabis!
Thus Spoke Zarathustra by Friedrich Nietzsche
Breeding is not just a method ; is also an empathy! Cannabis is there for helps!
Thus Spoke Zarathustra by Friedrich Nietzsche
Breeding is not just a method ; is also an empathy! Cannabis is there for helps!
G.O. Joe,
Just wanted to say thanks for all the links you posted to make it easier for people interested in reading the articles.
Now we need someone to organize by dates and subjects and to rate the articles. If there were abstracts and links, you are set.
My brain is just plain getting thicker, and slower and I am not so motivated as I used to be. But I know others are motivated and willing...
-SamS
Just wanted to say thanks for all the links you posted to make it easier for people interested in reading the articles.
Now we need someone to organize by dates and subjects and to rate the articles. If there were abstracts and links, you are set.
My brain is just plain getting thicker, and slower and I am not so motivated as I used to be. But I know others are motivated and willing...
-SamS
Excelent thread!
just wanted to thank Sam, G.O. Joe and all the colaboratos, really good info, i add it to my markers!!
Thanks
just wanted to thank Sam, G.O. Joe and all the colaboratos, really good info, i add it to my markers!!
Thanks
Could you please post it?
Clicking on this link takes me to the main page....
Quantitive analysis of Cannabinoids- https://www.icmag.com/ic/showthread....3725#post853752
Quantitive analysis of Cannabinoids- https://www.icmag.com/ic/showthread....3725#post853752
