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Best Healthiest non smoke form? Hash Oil, RSO, Honey, Kif,etc - Treating pain and cancer

acespicoli

Well-known member
Smokin Moose

How to extract the essence of cannabis and keep it in a little glass bottle. @pipeline

A tincture is a medicinal extract in an alcohol solution. The alcohol is used to extract and preserve the resins and other soluble material from the plant. Cannabis tinctures are an excellent way to utilize the plant's medicinal ingredients, and a perfect alternative for those who find smoking difficult.

Until the 1920's, Cannabis Indica tincture was available at your neighborhood pharmacy. Queen Victoria used medicinal cannabis extracts to deal with chronic pain. "Good for what ails ya," cannabis tinctures and extracts commonly served as analgesics, sedatives and narcotics.

Tinctures connect us to our pharmacological past – sepia-toned prescription memories and faded daguerreotypes of ancient potions, forgotten remedies, salves, lotions, ointments, syrups and miraculous elixirs.

Curious Alice pondered a small corked bottle labeled "Drink Me" in Lewis Carroll's 1864 classic Alice's Adventures Underground. "Well at least it doesn't say 'Poison,'" Alice wisely reflected. Yet Victorian magician Aleister Crowley's bottle of laudanum was indeed labeled "Poison," to impart a sense of danger and magic.

During the 18th century, absinthe drinkers mixed their wormwood extract with water, transforming it into the "Green Faerie" – a thujone-rich instant psychedelic drink.

The alchemy of alcohol

Alchemy comes from the Arabic word Al-Kimiya. It is a scientific discipline with spiritual and mystical components connected to ancient teachings of metallurgy, smelting, chemistry, pharmacology and biology. Alchemy is the stepchild of chemistry and physics.

Alchemy and tincture medicines share a common heritage. Potions and poisons are all derived from plants grown and carefully tended. The ritual comes full circle in the growing, preparing, and ingesting of the plant medicine – our bodies become the alchemical alembic.

Paraceselus, a 15th century Swiss alchemist, made medicinal tinctures and adopted the Arabic term al-kohl. He is also said to have invented laudanum, tincture of o***m.

Alchemical procedures involve both wet and dry forms such as soaking, distillation and evaporation. The extraction of entheogenic plants requires similar steps. Distillation remained undiscovered until the 12th century, when alchemists first created aqua vitae – aqueous alcohol concentrated by distillations.

In the ancient world, wines derived from fermentation reached a maximum of only 14%. Yet Graeco-Roman wines were customarily diluted by three or four parts, sometimes even by eight, and Homer mentions that cutting 20 parts of water to wine is sufficient for deep intoxication. It is obvious that an exceptionally potent concoction was being consumed. Indeed, the wines of Greek and Roman Bacchanalia were commonly infused with psychoactive plants to make a kind of vinous tincture.

The herbs were macerated in distilled spirits and then distilled again. Sometimes the distillate was then reinfused with a second batch of herbs.

Fruits were also used, along with minerals and precious stones like ground pearls, lapis lazuli, and gold leaf. Sometimes animals were used to make "man's brains" and "viper" wine.

Bee tincture, listed in an early 1900 edition of Practical Formularies, involved capturing and drowning live bees in spirits. Scorpion tincture requires soaking the arachnid for six months in pure grain alcohol, resulting in a nutty flavored restorative. Scorpions feature often in Chinese medicine, along with snakes and other exotica.


Alcohol availability

Cannabis tincture works best with 90% pure alcohol, such as Everclear.

Mexican tincture, however, uses only 35% pure, while Parke Davies Pharmaceuticals – distributors of the original medicinal pharmaceutical extract to pharmacies during the late 19th century – used 80%.

Pure grain alcohol can be difficult to obtain, depending on where you are located. If it's unavailable at your local liquor store, try a duty-free shop.

Most Canadian provinces sell only the highest potency navy rum at 75 proof (32% pure). Further distillation will increase the purity.

Confusion often exists around "proof" and "percent." Percent is approximately half to proof. The origins of the term "proof" hearkens back to the practice of testing alcohol content by wetting gunpowder with it then lighting the powder. If the alcohol was more than 50% water, the explosive would not ignite.

When the gunpowder did spark it was "proof" that the booze in question was at least half alcohol. In the US, 100 proof is defined as 50% alcohol by volume.

Exercise extreme caution when evaporating high-proof alcohol due to its flammable nature. Remember "proof and poof!"

How to make tincture

The philosophy behind tincture is to capture the spiritual and physical essence of the plant. This is done by using the power of ethyl alcohol to dissolve and preserve the herb in question, in our case cannabis.

Ethyl alcohol, known as ethanol, is used for countless applications. Produced biologically by the fermentation of either sugar or starch, ethanol may be used as a solvent for organic chemicals, or as a starting compound for manufacturing dyes, drugs, perfumes, and explosives.

Different plant species demand different strengths of solvent or alcohol. For example, o***m requires 70% pure grain for effective alkaloid leaching. Resinous plants such as cannabis, along with countless other alkaloid-rich botanicals, are ideally suited for extraction in high-proof spirits such as 90% pure grain alcohol (such as Everclear).

The cannabis used for soaking must be dry. When fresh bud is used, the end result is disappointing. Scissoring up the plant material effectively facilitates extracting all psychoactive constituents.

Cannabis should soak anywhere from one to 10 days. Some folks soak it for up to four weeks, following that up with a secondary five day soak in fresh ethanol just to ensure all cannabinoids have been leached.

However, some others insist that the buds remain in the solvent no longer than six hours. They claim that solvents instantly grab onto THC molecules, and anything after this time frame benefits only terpene, oils, and chlorophylls, contaminating the final product.

From my personal experience seven days is adequate, but you should experiment with different time frames to see what works for you and your buds.
The recommended minimum cannabis to alcohol ratio is one gram of bud per 35ml (one fluid ounce). Some prefer up to seven grams per 35ml but others might find this too strong. Individual needs vary. Cautious experimentation is the key.

Throughout the soaking period use only enough ethanol to cover the plant material and occasionally agitate. In a nod to the Goddess, herbs still in solution at this stage are referred to as the "Menstruum."

After you've soaked the bud for the desired time, shake and strain the plant material. After filtering the cannabis solution, it is ready to be stored. This is done best within a blue apothecary medicine bottle. This will protect the precious mixture from degradation by light, while also imbuing mystery to the potion.

For further protection, the tincture should be kept in a cool, dark place. Yet cannabis preserved in ethanol has a long shelf life. Tincture medicines do not come with an expiry date. The fragrance and bouquet of mature tincture is floral akin to perfume.

As the mother tincture matures, new cannabis solution is added, and the final evaporated concentrate extract becomes a composite of many superior cannabis flowers.

The test for making sure all THC has been transferred into ethanol is to smoke the discarded plant material. If it tastes terrible, has a straw color, and is inactive, the operation has been successful.

The spent bud should be collected in a porcelain bowl for drying before mulching. Over several deposits, however, you will notice a residue mark from essential extracted cannabinoids adhering to the sides of the collection bowl. Simply add a small amount of alcohol, swirl, and redissolve this valuable material back into the original mother tincture. Waste not want not!

How to use and consume

Cannabis tincture tastes positively Dionysian, with the sweet earthy flavor of cannabinoids and a lingering bitter aftertaste. The effects are noticeable within 15 minutes, and are felt completely within a half-hour.

An advantage of tincture and extract preparations is their ease of dispensing, consumption, and rapid absorption. Tinctures can become very potent when concentrated, so adjust according to individual dosage requirements.

Tincture comes on fast but soon flattens out, unlike the sustained build and longevity of cooked cannabis products. Throughout the tincture experience one is imbued with great tranquility, able to drift in and out of contemplative reverie.

Care must be exercised, as the delayed onset time may possibly encourage overdosing among those unfamiliar with cannabis tinctures. Orally administered cannabis products may be very uncomfortable when too much has been consumed. Possible panic and anxiety reactions or physical malaise may occur.

Heating the potion may also increase tincture strength. Tincture can be added to cooking recipes by concentrating the tincture into a syrup consistency, further enhancing efficacy. This becomes a commitment when considering the tincture experience's duration and extended nature.

The imbibing vessel or chalice should be reserved for "ceremonial" tincturing. An accompanying dropper for drawing extract, along with small flask for dispensing water into the libation glass is necessary. Tincture first goes into the glass, followed by the water. Glorious green cannabis quintessence explodes upon mixing with water into cloudy green opalescence.

Alcohol awareness

Ethanol represents the least toxic of all the alcohols. The toxicity of medicines, drugs, and poisons is calibrated by the LD50, meaning the lethal dose for 50 percent, signifying the amount of substance necessary to be fatal. Alcohol is considered in the highly dangerous category. Just five times the amount needed to get you happy can be lethal.

Unlike cannabis, ethanol shares no receptor sites to which it connects. Alcohol intoxication represents a true poisoning rather than a key to our cerebral natural paradises. Humans possess an enzyme called alcohol dehydrogenase which helps metabolize ethanol by oxidizing it to acetaldehyde. Other alcohols like methanol, propanol, phenol, and ethylene are extremely poisonous and can cause blindness and death.

The term "denatured alcohol" means poisonous methanol has been added to prevent drinking, rendering it unsuitable for tinctures ingested orally. When considering dealing with pure grain spirits, it is essential to dilute it with an equal part of water. I once subjected myself to an 0.70ml squirt of 95% ethanol – my upper palette remained sensitive for days.

When it comes to making tinctures, solvent alcohols are essential. These methods require high-proof spirits. Although in small amounts they are indispensable to the extraction process, some individuals may have difficulty with this dual relationship and possibly slippery slope.

For example, absinthe drinkers regularly consumed high alcohol-containing beverages along with their psychoactive thujone-rich wormwood extract. As well, many poets from the Romantic and Gothic periods entertained the additional drug ethanol along with their daily dosages of opiate-ladened laudanum. Thomas D'Quincey's Confessions of an English O***m Eater eloquently portrays the heaven and hell of this dual addiction.

So be careful that you use alcohol for its true purpose, as a carrier for the essence of magic plants, and not for its poisonous intoxication.

Personal experience

A standard tincture is at a ratio of one gram of cannabis to 35ml (1 ounce) of pure grain ethanol. At this strength, a dosage of 1.4ml of extract (2 squirts) mixed with water is barely noticeable, although it is a very effective appetite stimulant.

A tincture of seven grams of cannabis flowers suspended in 50ml (1.5 ounces) of ethanol is definitely psychoactive at a dosage of 0.7oml (one squirt). Upon doubling the dosage to 1.4ml, I achieved a Buddha-trance state, ideal for serious meditation. It was a heavy physical sensation to experience, and I was able to go in and out of meditative state. I felt a warm glow to my extremities.

At this strength, a "heroic dose" is five squirts for the five points of the pentacle and five wounds of Christ. I felt a heightened sense of novelty, an oceanic quiet and inner peace. I was very contemplative.

Often a small amount of cannabis smoked after tincturing greatly potentiates the experience, but the desire for further cannabis smoking is usually lessened.

by Stan Czolowski
 
Last edited:

acespicoli

Well-known member

Bend Over, It's Time To Get High - The Low Down on Cannabis Suppositories​

1722340211722.png


Smokin Moose

Fallen Cannabis Warrior & Ex Moderator​

A most outrageous profession.

While at the Expo, Dan comes up with the most hilarious product idea I’ve ever heard. “My company is Cannabanus,” he says in his best Dutch accent, “and my product is the new cannabis suppositories. You put the cannabis into the plug, the plug goes into your butt. Then you are getting high very quickly and efficiently. There are many blood vessels in the anus and they are absorbing the THC at an extremely fast rate. It’s called the Assinator.”
suppos_closeup.jpg

How to make Suppositories
To make marijuana caps, buy capsules at a homeopathic pharmacy or through the internet. The vendor will also have a small manual capper that makes 100 capsules at a time. The device holds one piece of the cap in place so they can be filled easily. There are several sizes. The biggest is a double O. Some people find these hard to swallow. A smaller size capsule is easier to swallow. It also makes it easier to take the right amount, not too much and not too little.

The cannabis is dried and ground to a very fine consistency in a coffee grinder. After the cannabis is ground, let it sit for a few minutes so that the THC bearing glands settle, rather than float away.

Pour the ground cannabis into a small bowl. Add enough olive oil and mix it in so that the cannabis powder sticks together. Use this mixture to fill the capsules, then put the tops on. Keep refrigerated. For long-term storage, place the capsules in a container and keep frozen.

Any grade of cannabis can be used. Renowned author Tom Flowers, deceased, first made caps from medium-sized leaves trimmed during manicuring. Later he switched over to smaller trim collected from the last stages of preparing bud. Both the quality and the quantity affect the intensity of the experience.

Another method that you might use to get high without smoking is using a tincture administered sublingually, that is, under the tongue. Cannabinoids that have been extracted and dissolved in alcohol are quickly absorbed by the mucus membranes.

If you really want to have a blast, fill your capsule with Medical Marijuana's bum ringing blackout bud butter.


Here is the recipe

Black Out Bud Butter
An even better bud bum buster butter.

Improvement number one comes from using European sweet butter. Regular butter is about 80% butterfat while European butters range from 84-86%. This may seem trivial but remember it is the butterfat that exacts the cannabinoids so a 7-8 % increase in butterfat will result in a more efficient extraction and stronger butter. European style butter comes from a number of manufacturers including Plugra from upstate New York, Strauss Organic, and recently Challenge European Style. Yes, the best still comes from France but it is exorbitantly priced.

Improvement number two comes from using a Braun high speed coffee grinder to convert all cannabis plant material from whatever source to a fine powder. The use of powder means the butter will taste more like cannabis as some of the chlorophyll and a few terpenes get into the butter. It also means a vastly increased surface area where the butterfat meets the cannabinoids. Using powder or “flour” significantly increases the potency of the final butter.

Improvement number three comes from using the best starting materials available. In this case Train Wreck, California Orange, and Pooh Bear trim were ground up and supplemented by powdered California Orange flowers. Using bud as opposed to trim will increase the potency of the butter.

The three improvements noted above resulted in cannabutter that is markedly different from the normal (and quite wonderful) Better Bud Butter. Black Out Bud Butter (BOBB) is approximately 2oo-300% stronger. The name BOBB comes from the fact that this butter is midnight green and will cause blackouts if too much is consumed.

Ingredients:

2 ½ pounds (six cups) of European Style high butterfat unsalted butter. I combined 8 oz of Plugra with 1 pound of Strauss Organic and 1 pound of Challenge European Style. Everyone has there own favorites but mine is Strauss. Strauss is smoother and less greasy than the others.

4 oz powdered Train Wreck Trim

2 oz powdered Pooh Bear Trim (a cross of Train Wreck and Salmon Creek Big Bud)

2 oz powdered California Orange trim

1/3 oz powdered California Orange flowers

Method:

The butters were melted in a covered crock pot set on high. The powdered cannabis was gradually stirred in with a wood spoon. The mixture was frequently stirred and cooked covered on high for one hour followed by three hours set on low. Stirring was accomplished every 15 minutes.

After four hours the hot mixture was squeezed through cheese cloth into a bowl then the product was filtered once more through cheese cloth. All available butter was squeezed out producing approximately 3 ½ cups. Theoretically, the cloth balls remaining could be re-extracted with more butter but the product would not be nearly as strong. Approximately 75-85% of the available cannabinoids are extracted on the first pass.

Note on Preparation:

No gloves were used in squeezing the rather hot cloth balls into the collection vessel. If you go barehanded beware of burns. Usually the ball can be twisted and held at the top then you can press the ball with the wood spoon against the side of the collection vessel. Once the ball has cooled sufficiently you can “wring” out the last of the cannabutter. Yes, this is messy but will leave your hands oh so soft.

Note on Amount of powdered cannabis to use: I use as much as will go into solution with the butter. This varies depending upon the cannabis and source.

Note on Potency:

I know this cannabutter is potent simply from the extreme effects produced from handling it
 

CharlesU Farley

Well-known member
Smokin Moose

How to extract the essence of cannabis and keep it in a little glass bottle. @pipeline

A tincture is a medicinal extract in an alcohol solution. The alcohol is used to extract and preserve the resins and other soluble material from the plant. Cannabis tinctures are an excellent way to utilize the plant's medicinal ingredients, and a perfect alternative for those who find smoking difficult.

Until the 1920's, Cannabis Indica tincture was available at your neighborhood pharmacy. Queen Victoria used medicinal cannabis extracts to deal with chronic pain. "Good for what ails ya," cannabis tinctures and extracts commonly served as analgesics, sedatives and narcotics.

Tinctures connect us to our pharmacological past – sepia-toned prescription memories and faded daguerreotypes of ancient potions, forgotten remedies, salves, lotions, ointments, syrups and miraculous elixirs.

Curious Alice pondered a small corked bottle labeled "Drink Me" in Lewis Carroll's 1864 classic Alice's Adventures Underground. "Well at least it doesn't say 'Poison,'" Alice wisely reflected. Yet Victorian magician Aleister Crowley's bottle of laudanum was indeed labeled "Poison," to impart a sense of danger and magic.

During the 18th century, absinthe drinkers mixed their wormwood extract with water, transforming it into the "Green Faerie" – a thujone-rich instant psychedelic drink.

The alchemy of alcohol

Alchemy comes from the Arabic word Al-Kimiya. It is a scientific discipline with spiritual and mystical components connected to ancient teachings of metallurgy, smelting, chemistry, pharmacology and biology. Alchemy is the stepchild of chemistry and physics.

Alchemy and tincture medicines share a common heritage. Potions and poisons are all derived from plants grown and carefully tended. The ritual comes full circle in the growing, preparing, and ingesting of the plant medicine – our bodies become the alchemical alembic.

Paraceselus, a 15th century Swiss alchemist, made medicinal tinctures and adopted the Arabic term al-kohl. He is also said to have invented laudanum, tincture of o***m.

Alchemical procedures involve both wet and dry forms such as soaking, distillation and evaporation. The extraction of entheogenic plants requires similar steps. Distillation remained undiscovered until the 12th century, when alchemists first created aqua vitae – aqueous alcohol concentrated by distillations.

In the ancient world, wines derived from fermentation reached a maximum of only 14%. Yet Graeco-Roman wines were customarily diluted by three or four parts, sometimes even by eight, and Homer mentions that cutting 20 parts of water to wine is sufficient for deep intoxication. It is obvious that an exceptionally potent concoction was being consumed. Indeed, the wines of Greek and Roman Bacchanalia were commonly infused with psychoactive plants to make a kind of vinous tincture.

The herbs were macerated in distilled spirits and then distilled again. Sometimes the distillate was then reinfused with a second batch of herbs.

Fruits were also used, along with minerals and precious stones like ground pearls, lapis lazuli, and gold leaf. Sometimes animals were used to make "man's brains" and "viper" wine.

Bee tincture, listed in an early 1900 edition of Practical Formularies, involved capturing and drowning live bees in spirits. Scorpion tincture requires soaking the arachnid for six months in pure grain alcohol, resulting in a nutty flavored restorative. Scorpions feature often in Chinese medicine, along with snakes and other exotica.


Alcohol availability

Cannabis tincture works best with 90% pure alcohol, such as Everclear.

Mexican tincture, however, uses only 35% pure, while Parke Davies Pharmaceuticals – distributors of the original medicinal pharmaceutical extract to pharmacies during the late 19th century – used 80%.

Pure grain alcohol can be difficult to obtain, depending on where you are located. If it's unavailable at your local liquor store, try a duty-free shop.

Most Canadian provinces sell only the highest potency navy rum at 75 proof (32% pure). Further distillation will increase the purity.

Confusion often exists around "proof" and "percent." Percent is approximately half to proof. The origins of the term "proof" hearkens back to the practice of testing alcohol content by wetting gunpowder with it then lighting the powder. If the alcohol was more than 50% water, the explosive would not ignite.

When the gunpowder did spark it was "proof" that the booze in question was at least half alcohol. In the US, 100 proof is defined as 50% alcohol by volume.

Exercise extreme caution when evaporating high-proof alcohol due to its flammable nature. Remember "proof and poof!"

How to make tincture

The philosophy behind tincture is to capture the spiritual and physical essence of the plant. This is done by using the power of ethyl alcohol to dissolve and preserve the herb in question, in our case cannabis.

Ethyl alcohol, known as ethanol, is used for countless applications. Produced biologically by the fermentation of either sugar or starch, ethanol may be used as a solvent for organic chemicals, or as a starting compound for manufacturing dyes, drugs, perfumes, and explosives.

Different plant species demand different strengths of solvent or alcohol. For example, o***m requires 70% pure grain for effective alkaloid leaching. Resinous plants such as cannabis, along with countless other alkaloid-rich botanicals, are ideally suited for extraction in high-proof spirits such as 90% pure grain alcohol (such as Everclear).

The cannabis used for soaking must be dry. When fresh bud is used, the end result is disappointing. Scissoring up the plant material effectively facilitates extracting all psychoactive constituents.

Cannabis should soak anywhere from one to 10 days. Some folks soak it for up to four weeks, following that up with a secondary five day soak in fresh ethanol just to ensure all cannabinoids have been leached.

However, some others insist that the buds remain in the solvent no longer than six hours. They claim that solvents instantly grab onto THC molecules, and anything after this time frame benefits only terpene, oils, and chlorophylls, contaminating the final product.

From my personal experience seven days is adequate, but you should experiment with different time frames to see what works for you and your buds.
The recommended minimum cannabis to alcohol ratio is one gram of bud per 35ml (one fluid ounce). Some prefer up to seven grams per 35ml but others might find this too strong. Individual needs vary. Cautious experimentation is the key.

Throughout the soaking period use only enough ethanol to cover the plant material and occasionally agitate. In a nod to the Goddess, herbs still in solution at this stage are referred to as the "Menstruum."

After you've soaked the bud for the desired time, shake and strain the plant material. After filtering the cannabis solution, it is ready to be stored. This is done best within a blue apothecary medicine bottle. This will protect the precious mixture from degradation by light, while also imbuing mystery to the potion.

For further protection, the tincture should be kept in a cool, dark place. Yet cannabis preserved in ethanol has a long shelf life. Tincture medicines do not come with an expiry date. The fragrance and bouquet of mature tincture is floral akin to perfume.

As the mother tincture matures, new cannabis solution is added, and the final evaporated concentrate extract becomes a composite of many superior cannabis flowers.

The test for making sure all THC has been transferred into ethanol is to smoke the discarded plant material. If it tastes terrible, has a straw color, and is inactive, the operation has been successful.

The spent bud should be collected in a porcelain bowl for drying before mulching. Over several deposits, however, you will notice a residue mark from essential extracted cannabinoids adhering to the sides of the collection bowl. Simply add a small amount of alcohol, swirl, and redissolve this valuable material back into the original mother tincture. Waste not want not!

How to use and consume

Cannabis tincture tastes positively Dionysian, with the sweet earthy flavor of cannabinoids and a lingering bitter aftertaste. The effects are noticeable within 15 minutes, and are felt completely within a half-hour.

An advantage of tincture and extract preparations is their ease of dispensing, consumption, and rapid absorption. Tinctures can become very potent when concentrated, so adjust according to individual dosage requirements.

Tincture comes on fast but soon flattens out, unlike the sustained build and longevity of cooked cannabis products. Throughout the tincture experience one is imbued with great tranquility, able to drift in and out of contemplative reverie.

Care must be exercised, as the delayed onset time may possibly encourage overdosing among those unfamiliar with cannabis tinctures. Orally administered cannabis products may be very uncomfortable when too much has been consumed. Possible panic and anxiety reactions or physical malaise may occur.

Heating the potion may also increase tincture strength. Tincture can be added to cooking recipes by concentrating the tincture into a syrup consistency, further enhancing efficacy. This becomes a commitment when considering the tincture experience's duration and extended nature.

The imbibing vessel or chalice should be reserved for "ceremonial" tincturing. An accompanying dropper for drawing extract, along with small flask for dispensing water into the libation glass is necessary. Tincture first goes into the glass, followed by the water. Glorious green cannabis quintessence explodes upon mixing with water into cloudy green opalescence.

Alcohol awareness

Ethanol represents the least toxic of all the alcohols. The toxicity of medicines, drugs, and poisons is calibrated by the LD50, meaning the lethal dose for 50 percent, signifying the amount of substance necessary to be fatal. Alcohol is considered in the highly dangerous category. Just five times the amount needed to get you happy can be lethal.

Unlike cannabis, ethanol shares no receptor sites to which it connects. Alcohol intoxication represents a true poisoning rather than a key to our cerebral natural paradises. Humans possess an enzyme called alcohol dehydrogenase which helps metabolize ethanol by oxidizing it to acetaldehyde. Other alcohols like methanol, propanol, phenol, and ethylene are extremely poisonous and can cause blindness and death.

The term "denatured alcohol" means poisonous methanol has been added to prevent drinking, rendering it unsuitable for tinctures ingested orally. When considering dealing with pure grain spirits, it is essential to dilute it with an equal part of water. I once subjected myself to an 0.70ml squirt of 95% ethanol – my upper palette remained sensitive for days.

When it comes to making tinctures, solvent alcohols are essential. These methods require high-proof spirits. Although in small amounts they are indispensable to the extraction process, some individuals may have difficulty with this dual relationship and possibly slippery slope.

For example, absinthe drinkers regularly consumed high alcohol-containing beverages along with their psychoactive thujone-rich wormwood extract. As well, many poets from the Romantic and Gothic periods entertained the additional drug ethanol along with their daily dosages of opiate-ladened laudanum. Thomas D'Quincey's Confessions of an English O***m Eater eloquently portrays the heaven and hell of this dual addiction.

So be careful that you use alcohol for its true purpose, as a carrier for the essence of magic plants, and not for its poisonous intoxication.

Personal experience

A standard tincture is at a ratio of one gram of cannabis to 35ml (1 ounce) of pure grain ethanol. At this strength, a dosage of 1.4ml of extract (2 squirts) mixed with water is barely noticeable, although it is a very effective appetite stimulant.

A tincture of seven grams of cannabis flowers suspended in 50ml (1.5 ounces) of ethanol is definitely psychoactive at a dosage of 0.7oml (one squirt). Upon doubling the dosage to 1.4ml, I achieved a Buddha-trance state, ideal for serious meditation. It was a heavy physical sensation to experience, and I was able to go in and out of meditative state. I felt a warm glow to my extremities.

At this strength, a "heroic dose" is five squirts for the five points of the pentacle and five wounds of Christ. I felt a heightened sense of novelty, an oceanic quiet and inner peace. I was very contemplative.

Often a small amount of cannabis smoked after tincturing greatly potentiates the experience, but the desire for further cannabis smoking is usually lessened.

by Stan Czolowski
I decarb before I make a tincture, because once the tincture hits under my tongue, I start salivating profusely and eventually end up swallowing some of it.
 

acespicoli

Well-known member

Medicinal properties of terpenes found in Cannabis sativa and Humulus lupulus


Cite
https://doi.org/10.1016/j.ejmech.2018.07.076

Section snippets​

Myrcene​

Myrcene (MYR, the molecular structure of which is shown in Fig. 1A) is frequently the most abundant terpene encountered in cannabis and hops. For instance, the total terpenes of the cannabis drug chemotype 'blueberry' consist of up to 78% MYR [13]. Furthermore, some drug chemotypes, possibly due to the founder effect and selective breeding, show a high but stable composition with respect to this terpene; for instance, the medical cannabis chemotype, with low THC and high CBD, also known as

β-caryophyllene​

β-caryophyllene (BCP, Fig. 1B) is frequently the predominant terpenoid in cannabis and present in hops. For instance, it comprises 64% of terpenes in the cannabis drug chemotype 'gorilla glue' [13], but is almost absent (1.2 ± 0.2%) in some other samples [27]. It is typically less abundant in hops, making up to 15% of the EO of a wild-growing hop [3]. It is also widely present in a large number of plants e.g. clove, rosemary, black pepper and lavender. Unlike any other terpenes, BCP has a

Caryophyllene oxide​

The oxidized derivative of BCP, caryophyllene oxide (BCPO, Fig. 1C), is also found in plants outside of Cannabaceae, such as guava (Psidium guajava), oregano (Origanum vulgare), cinnamon (Cinnamomum spp.) clove (Eugenia caryophyllata), black pepper (Piper nigrum), lemon balm (Melissa officinalis) and eucalyptus (Melaleuca stypheloides), whose EO contains 44% BCPO [58]. BCPO is a non-toxic and non-sensitizing agent ([10] and refs), which is often used as a preservative in foods, drugs and

Humulene​

Humulene (HUM, Fig. 1D) is one of the predominant terpenes in C. sativa and H. lupulus: it makes up 52% [3] and 19 ± 7.6% [5] of their total volatile fraction, respectively. It is widely found across the planta e.g. sage, ginseng and Syzygium zeylanicum (Myrtaceae). HUM is also called α-caryophyllene, but it does not contain the cyclobutane ring as β-caryophyllene does, and has not yet been characterized as a CB2 ligand.

However, HUM possesses anti-inflammatory and anticancer properties. A study

α-Pinene​

α-Pinene (α-PN, Fig. 2A) is the most widely encountered terpenoid in nature, since it is found in conifers in large amounts. It is sometimes the dominant terpene in cannabis; for instance, an inflorescence sample of the drug chemotype 'bubba hash' contained 48% α-PN in the total terpenes [13] and the hemp cultivar finola contained 23 ± 17% [5]. It can be also found in the essential oils of salvia, Spanish sage, black plum and lesser galangal, etc. Interestingly, α-PN is source material for the

β-Pinene​

β-PN (Fig. 2B) is one of the most abundant compounds released by conifers, but it is regularly found in cannabis (6.1 ± 0.4%) [27] and hop cultivars in moderate amounts [93]. It is found also in e.g. Cuminum cyminum and Clausena anisata. In contact with air, it is oxidized to pinocarveol and myrtenol and other molecules and it is easily converted to other terpenes.

β-PN (100 mg/kg) showed antidepressant and sedative activities in mice with several experimental models [94]. Elsewhere, it was

Linalool​

D-linalool (LNL, Fig. 2C) is rarely found as a predominant terpene in cannabis; e.g. 25% of all terpenes found in the “Sour OG” drug chemotype [13]. It is a constituent of hop EO at least in minor amounts [99]. Lavender (Lavandula angustifolia) EO can contain around 50% linalyl acetate and 35% LNL; of particular note is that linalyl acetate is readily converted to LNL in the gastric system. Lavender (EO) is traditionally used for relaxation, treating parasitic infections, burns, insect bites

Limonene​

Limonene (LIM, Fig. 2D) is occasionally the predominant terpene in Cannabis; for instance, in the 'girl scout cookie' drug chemotype, it comprised 56% of all terpenes [13], whereas in hops, it seems to be less abundant. However, it is also found in lemon rind and in other citrus (up to 97%), ajwain, Bupleurum gibraltarium (up to 96%), celery (up to 66%), ebolo (up to 70%), Canadian horseweed (up to 70%), and Bolivian coriander (up to 75%) essential oils. R-limonene and d-limonene are

Perillyl alcohol​

While present in Cannabaceae, Perillyl Alcohol (POH, Fig. 2E) is an abundant terpene in lavender, sage, and peppermint, and especially in the EOs of mints, cherries, citrous fruits and lemon grass. It is common ingredient in cleaning products and cosmetics. It is also a metabolite of LIM via hydroxylation by cytochrome P450 enzymes. It has no known toxicities. POH and its derivatives hold strong promises in cancer treatments, especially against brain tumors [145]. These are under many

Terpinolene​

Terpinolene (TPL, Fig. 3A) is also known as δ-Terpinene due to its close similarity to other terpinenes. It is sometimes the chief terpene found in a cannabis sample e.g. in the 'durban poison' drug chemotype (55%) [13]. It has been found in a variety of plant sources such as sage, apple, cumin, lilac, tea tree and lemon, but it is primarily isolated from pine and fir trees.

Downstream of PI3K signaling, Akt is a regulatory protein involved in the metabolism, proliferation, cell survival, growth

γ-Terpinene​

In addition to cannabis and hops, γ-Terpinene (γ-TPN, Fig. 3B) has been isolated from a variety of plant sources such as savories (Satureja) and thyme. Terpene is used as a flavoring agent and is not acutely toxic at least at 2 g/kg in rats [180]. γ-TPN (1.6–50 mg/kg) showed an anti-nociceptive effect in the formalin, capsaicin, and glutamate-induced pain models in rats [180]. When the animals were pretreated with naloxone, glibenclamide, atropine and mecamylamine in the glutamate test, they

α-Terpinene​

α-Terpinene (α-TPN, Fig. 3C) is found in cannabis and hops and is commonly used as a fragrance compound. It is found in allspice and many EOs e.g. from tea tree and Litsea ceylanica (20%), but it is usually produced industrially from α-pinene. α-TPN shows no embryofetotoxicity following the oral administration of 30 mg/kg to rats [186] and is also not mutagenic according to the Ames tests [187]. α-TPN shows good ROS scavenging activity, trapping approximately 0.7 radicals when protecting

Terpineols​

α- and γ-terpineols (TOLs, molecular structure of α-terpineol is presented in Fig. 3C), and terpinen-4-ol (T4OL, Fig. 3E) isomers are likely derived from their terpinene counterparts by hydration in plants. They are present in a variety of plant sources such as tea tree oil, cajuput oil, pine oil, and petitgrain oils, lilacs, pine trees, lime blossoms and eucalyptus. T4OL is present at high concentrations (30–48%) in tea tree EO and at up to 29% in lavender EO [192]. They can be synthesized

Geraniol​

Geraniol (GOH, Fig. 4A) is used in perfumes and as a flavoring agent, and also as a preservative, insect repellent and attractant. It is the chief component of palmarosa oil and the second most abundant in rose oil, as well as being found in citronella oils (lemon grass) and Geranium. Actually, it is found at least in 160 plant EOs (references in Ref. [223]) including cannabis and hops. GOH is important in the biosynthesis of other terpenes. The LD50 value of GOH is 3.6 g/kg in rats [224]. To

Nerolidol​

Nerolidol (NOH, Fig. 4B) is herbivore and pathogen-induced volatile in plants, but it is regularly found across the planta, including Cannabaceae, and makes up 74–95% of the total terpenes in paper bark tea tree oil. It is found in the EOs of the ginger family (up to 90%), Siparuna (90%), Myrceugenia (90%), Piper claussenianum (83%), Melaleuca quinquenervia (87%), New England peppermint tree (80%), Salvia (72%) and in neroli, from which it got its name. NOH is commonly used across industries

Borneol​

Borneol (BOH, Fig. 4C) is found up to 14% in cannabis cultivars [251] and can be found in several species of Artemisia, Blumea and Kaempferia and Dryobalanops and EOs of many medicinal herbs, such as valerian. It has low toxicity: the oral LD50 of BOH is 3000–5800 mg/kg in rodents and 3200 mg/kg in rabbits. BOH has been and is still used in traditional Chinese medicine formulations as a drug enhancer and to mitigate the effects of heart disease. Indeed, it may play a role in the control of

α-Bisabolol​

Bisabolol (BISA, Fig. 4D) is widely used in the cosmetics industry. BISA was sometimes the second most abundant terpene (17%) out of the 200 analyzed samples of cannabis [13] and is present in hops (at levels of up to 16%) [3]. In addition to cannabis and hops, this unsaturated monocyclic sesquiterpene alcohol – also known as levomenol – is found in EOs from the candeia tree, salvia, Plinia, Eremanthus and cat's claw. It is the main constituent in some Eos; for instance, more than 80% of the EO

Bisabolenes​

α-, β- and γ-bisabolene (Fig. 4E) are found – in addition to in cannabis and hops – in a wide variety of plants including cubeb, lemon and oregano. Various natural derivatives of bisabolenes can function as pheromones in a variety of insects e.g. in fruit flies. They are also produced by several fungi, but their biological role in fungi remains unknown. β-Bisabolene has a balsamic odor and is approved in Europe as a food additive. β- and γ-bisabolenes have so far been found to possess

β-elemene​

β-elemene (ELE, Fig. 5A) is found in wild hops from Lithuania at levels of up to 14% [3] and in notable amounts in the medical cannabis cultivar 'bedropuur' [324]. It contributes to the floral aromas of many plants and is used as a pheromone by some insects. It is also found in the Chinese medical herb Rhizoma zedoariae, which has been used for its alleged properties to improve blood circulation and alleviate pain.

The efficacy of ELE in cancer treatments was reviewed in 2013 [325] and 2017 [326

Fenchone​

In addition to cannabis and hops, fenchone (Fig. 4B) is found in fennel, olive leaves, flowering parts of Lavandula stoechas while its EO can contain 30% of fenchone. Fenchone is used as a flavoring agent in foods and in perfumery. Traditionally fennel products are used for the improvement of food digestion and the prevention of flatulence. Biological effects of fenchone per se have so far been limited to two studies. First, fenchone was able to inhibit carcinoma progression in vivo by inducing

Pulegone​

In addition to Cannabaceae, pulegone (PUL, Fig. 5C) is widely present in the Mentha genus e.g. in Mentha pulegium, from which PUL gets its name, and in Calamintha nepeta (lesser calamint) oil, which contains PUL up to 85% [347]. It is also found in Agastache formosanum oil and rosemary for instance. This monoterpene ketone is used as a flavoring agent and in perfumery. It is claimed to possess antispasmodic, emmenagogue, diaphoretic, diuretic and CNS strengthening properties. The biological

α-Phellandrene​

α-Phellandrene (α-PA, Fig. 5D), in addition to C. sativa and H. lupulus, is found also in Eucalyptus phellandra, from which it got its name. α-PA has also been isolated from the oil of water fennel and Canada balsam oil and Schinus terebinthifolius (rose pepper) fruits. The phellandrenes are used in fragrances and are approved as flavoring agents in the EU.

It has been reported that α-PA modulates immune responses in mice. A previous study [356] demonstrated that α-PA (25 mg/kg) increased the

β-eudesmol​

In addition to cannabis and hops [363], β-eudesmol (β-EOH, Fig. 5E) is present in Atractylodes lancea and Zingiber (gingers), for example. A. lancea is used in traditional Chinese medicine to ease gastrointestinal problems, eliminate pathogens and treat headaches, body aches, fever and blocked nasal passages. β-EOH exhibits pro-apoptotic, anti-proliferative and antitumor properties. β-EOH inhibited the proliferation of human lung (A549) and colon (HT29 and Caco-2) cancer cells in vitro [364].

Other terpenes found in cannabis and hops​

Isopulegol (Fig. 6A), which differs from menthol only by a single extra double bond, presented depressant- and anxiolytic-like effects when it was administered to male mice with the i. p. dose of 25 mg/kg [375]. The effect was similar to that of diazepam (1 mg/kg), but without the loss in general motor activity in the OFT. However, the overall sedative effect was likely due to a similar mechanism of action to that of benzodiazepine i.e. the positive modulation on GABAergic receptors. This was

Discussion​

The terpenes reviewed herein show very low acute toxicity: typically, acute oral LD50 values are around 5000 mg/kg or higher e.g. for β-caryophyllene, myrcene, limonene, terpinolene, pinenes, nerolidol, ocimenes and fenchone [411]. This means that a good therapeutic index (LD50 of 1%) is achieved with the administration of 50 mg/kg of these terpenes – a typical amount for the biological activity for terpenes reviewed herein. Even lower effective doses were regularly met in vivo, for instance

Conclusions​

Terpenes are widely used in industry, perfumery, as food additives and in traditional medicines. They show low toxicity and high bioavailability and readily cross the skin and BBB. They have a good therapeutic index i.e. they are well tolerated without side effects and the therapeutic effects are gained far before the lethal dose. Many terpenes exhibit high selectivity over receptors such as TRP channels, and dopaminergic and GABAergic receptors. Only β-caryophyllene show high affinity to...

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acespicoli

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pipeline

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Trying to provide latest news, and best resources in a single thread.
Hope someone finds this post useful :huggg:

Wonder where they are getting their cannabis for the studies?

Should use coconut oil kief capsules. Wonder if there are studies on delivery systems with different types of carrier oils in edibles/capsules?

 

acespicoli

Well-known member
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Have a look at the contents, you may want to pick up a copy :huggg:

CONTENTS

About the Editors xiii
Contributors xv
Foreword xvii
Introduction 1

Franjo Grotenhermen
PART I: HISTORICAL NOTES
Chapter 1. The Therapeutic Use of Cannabis sativa (L.)
in Arabic Medicine 5

Indalecio Lozano

Introduction 5
Materials and Methods 6
Results 6
Conclusion 10
Update 12
Chapter 2. Cognoscenti of Cannabis I: Jacques-Joseph
Moreau (1804-1884) 13

Ethan B. Russo

Chapter 3. Cognoscenti of Cannabis II: Simeon Seth
on Cannabis 17

David Deakle

Chapter 4. The Medical Use of Cannabis Among
the Greeks and Romans 23

James L. Butrica

Introduction 23
Absence of Mention 24
The Emergence of Cannabis 25
Appendix I: Passages Discussing Wild Cannabis Possibly
Misunderstood As Discussing Domesticated Cannabis 40

Appendix II: Cannabis in Veterinary Medicine 41
Update 42
Chapter 5. A Homelie Herbe: Medicinal Cannabis
in Early England 43

Vivienne Crawford

Introduction 43
Cannabis History in England 45
The Modern Era 49
Update 51
Chapter 6. Future of Cannabis and Cannabinoids
in Therapeutics 53

Ethan B. Russo

Introduction 53
New Indications for Cannabinoid Pharmaceuticals 56
Cannabinoids and Neuroprotection 57
Spasmodic Disorders 58
Forbidden Territories 58
Update 63
PART II: PHARMACOLOGY
AND PHARMACOKINETICS
Chapter 7. Clinical Pharmacokinetics
of Cannabinoids 69

Franjo Grotenhermen

Introduction 69
Pharmacokinetics of ∆9

-THC 74
Absorption 75
Distribution 80
Metabolism 84
Course of Plasma Concentration of THC
and Metabolites 87
Elimination 88
Time Effect Relationship 94
Pharmacokinetics of Other Cannabinoids 99
Metabolic Interactions 101
Conclusion 103

Chapter 8. Clinical Pharmacodynamics
of Cannabinoids 117

Franjo Grotenhermen

Introduction 117
Mechanism of Action 119
Cannabinoid Receptors 121
Endocannabinoids 122
Pharmacological Effects of THC 125
Toxicity 125
Psyche, Cognition and Behavior 127
Central Nervous System
and Neurochemistry 128
Circulatory System 130
Some Other Organ Systems and Effects 131
Pharmacological Activity
of THC Metabolites 133
Pharmacological Effects
of Other Cannabinoids 135
Tolerance and Dependency 138
Therapeutic Uses 139
Hierarchy of Therapeutic Effects 140
Basic Research Stage 141
Drug Interactions 143
Conclusions 144
Update 165

Chapter 9. Cannabis and Cannabis Extracts:
Greater Than the Sum of Their Parts? 171

John M. McPartland
Ethan B. Russo

Introduction 171
Materials and Methods 172
Results and Discussion 172
Cannabinoids 173
Terpenoids 178
Flavonoids 187
Conclusions 190
Update 201

PART III: ENDOCANNABINOIDS
AND CANNABINOID RECEPTORS
Chapter 10. The Endocannabinoid System: Can It
Contribute to Cannabis Therapeutics? 207

Vincenzo Di Marzo

The Endocannabinoid System 207
Endocannabinoid Pharmacology: More Than
Meets the Eye 209
Levels of Endocannabinoids in Tissues: Physiology
and Pathology 211
New Drugs From the Endocannabinoid System: Curative
or Palliative? 216
Update 225
Chapter 11. Cannabinoids and Feeding: The Role
of the Endogenous Cannabinoid System As a Trigger
for Newborn Suckling 227

Ester Fride

Interactions of the Endocannabinoid System
with Hormones Regulating Food Intake 228
Endocannabinoids in Food Substances 229
Developmental Aspects of the Endocannabinoid-CB1
Receptor System 230
Blockade of CB1 Receptors in Newborn Mice 231
Mechanisms of the CB1 Receptor Blockade-Induced
Growth-Stunting Effects 233
Conclusions 234
Update 238
PART IV: MEDICINAL USES
Chapter 12. Marijuana (Cannabis) As Medicine 243

Leo E. Hollister

Introduction 243
Indications with Evidence for Medical Efficacy 245
Indications with Sparse Evidence of Efficacy 253
Discussion 257
Conclusion 258
Update 263

Chapter 13. Effects of Smoked Cannabis and Oral 9
-Tetrahydrocannabinol on Nausea and Emesis
After Cancer Chemotherapy: A Review of State
Clinical Trials 265

Richard E. Musty
Rita Rossi

Tennessee 266
Michigan 267
Georgia 268
New Mexico (1983) 270
New Mexico (1984) 271
California 272
New York 273
Discussion 275
Update 278
Chapter 14. Hyperemesis Gravidarum and Clinical
Cannabis: To Eat or Not to Eat? 281

Wei-Ni Lin Curry

HG, Its Medicalization, and the Survivors 282
Cannabis, Pregnancy, and HG 288
Two Women’s Stories of Using Folk,
Alternative Medicine 293
Conclusion 297
Update 302
Chapter 15. Therapeutic Cannabis (Marijuana)
As an Antiemetic and Appetite Stimulant
in Persons with Acquired Immunodeficiency Syndrome 303

Richard E. Bayer

AIDS in the United States 303
Cannabinoids as Antiemetic and Appetite Stimulant
in AIDS Wasting Syndrome 305
Case Reports (The Patients’ Perspective) 306
Recent Clinical Research on Cannabinoids, Immunity,
and Weight Gain 308
Cannabis and Harm Reduction Strategies for Persons
with AIDS 308
Conclusion 311
Update 313

Chapter 16. Cannabis Treatments in Obstetrics
and Gynecology: A Historical Review 315

Ethan B. Russo

Introduction 315
The Ancient World and Medieval Middle and Far East 315
European and Western Medicine 318
Modern Ethnobotany of Cannabis in Obstetrics
and Gynecology 331
Recent Theory and Clinical Data 334
Discussion and Conclusions 338
Update 345
Chapter 17. Crack Heads and Roots Daughters:
The Therapeutic Use of Cannabis in Jamaica 347

Melanie Dreher

Ganja 348
Cocaine 350
Women and Crack 351
Conclusions 357
Implications 358
Update 360
Chapter 18. Cannabis in Multiple Sclerosis:
Women’s Health Concerns 363

Denis J. Petro

Introduction 363
Treatment Options: Acute Episodes, Disease Modification
and Symptom Management 364
Cannabis in Acute Treatment and Disease Modification 365
Cannabis in Symptom Management 367
Impaired Mobility: Spasticity 368
Tremor 369
Nystagmus 369
Postural Regulation 370
Fatigue 370
Pain 371
Bladder Dysfunction 371
Sexual Dysfunction 372
Discussion 372

Conclusions 373
Update 377
PART V: SIDE EFFECTS
Chapter 19. Cannabis and Harm Reduction:
A Nursing Perspective 383

Mary Lynn Mathre

Introduction 383
Cannabis Was a Medicine in the United States 385
Cannabis As a Harm Reduction Medicine 386
Cannabis As a Social/Recreational Drug 389
Cannabis Prohibition Causes More Harm Than the Drug 393
Conclusions 396
Chapter 20. Chronic Cannabis Use in the Compassionate
Investigational New Drug Program: An Examination
of Benefits and Adverse Effects of Legal Clinical
Cannabis 399
Ethan B. Russo Paul J. Bach
Mary Lynn Mathre Juan Sanchez-Ramos
Al Byrne Kristin A. Kirlin
Robert Velin
Introduction 400
Previous Chronic Cannabis Use Studies 400
A Brief History of the Compassionate IND 401
Methods 403
Results And Discussion 404
Conclusions and Recommendations 442
Update 447
Afterword 449
Index 451
 

acespicoli

Well-known member
Front. Plant Sci., 29 April 2019
Sec. Plant Breeding
Volume 10 - 2019 | https://doi.org/10.3389/fpls.2019.00476
This article is part of the Research TopicCannabis Genomics, Breeding and ProductionView all 17 articles

Polyploidization for the Genetic Improvement of Cannabis sativa

Cannabis Synergy​

In 1998, Professors Raphael Mechoulam and Shimon Ben-Shabat posited that the endocannabinoid system demonstrated an “entourage effect” in which a variety of “inactive” metabolites and closely related molecules markedly increased the activity of the primary endogenous cannabinoids, anandamide and 2-arachidonoylglycerol (Ben-Shabat et al., 1998). They also postulated that this helped to explain how botanical drugs were often more efficacious than their isolated components (Mechoulam and Ben-Shabat, 1999). Although the single molecule synthesis remains the dominant model for pharmaceutical development (Bonn-Miller et al., 2018), the concept of botanical synergy has been amply demonstrated contemporaneously, invoking the pharmacological contributions of “minor cannabinoids” and Cannabis terpenoids to the plant’s overall pharmacological effect (McPartland and Pruitt, 1999; McPartland and Mediavilla, 2001; McPartland and Russo, 2001, 2014; Russo and McPartland, 2003; Wilkinson et al., 2003; Russo, 2011). Several pertinent examples of the entourage effect in Cannabis are illustrative:

In a randomized controlled trial of oromucosal Cannabis-based extracts in patients with intractable pain despite optimized opioid treatment, a THC-predominant extract failed to demarcate favorably from placebo, whereas a whole plant extract (nabiximols, vide infra) with both THC and cannabidiol (CBD) proved statistically significantly better than both (Johnson et al., 2010), the only salient difference being the presence of CBD in the latter.

In animal studies of analgesia, pure CBD produces a biphasic dose-response curve such that smaller doses reduce pain responses until a peak is reached, after which further increases in dose are ineffective. Interestingly, the application of a full spectrum Cannabis extract with equivalent doses of CBD eliminates the biphasic response in favor of a linear dose-response curve such that the botanical extract is analgesic at any dose with no observed ceiling effect (Gallily et al., 2014).

A recent study of several human breast cancer cell lines in culture and implanted tumors demonstrated superiority of a Cannabis extract treatment to pure THC, seemingly attributable in the former to the presence of small concentrations of cannabigerol (CBG) and tetrahydrocannabinolic acid (THCA) (Blasco-Benito et al., 2018).

Anticonvulsant effects of cannabidiol were noted in animals in the 1970s with the first human trials in 1980 (Cunha et al., 1980). A recent experiment in mice with seizures induced by pentylenetetrazole employed five different Cannabis extracts with equal CBD concentrations (Berman et al., 2018). Although all the extracts showed benefits compared to untreated controls, salient differences were observed in biochemical profiles of non-CBD cannabinoids, which, in turn, led to significant differences in numbers of mice developing tonic-clonic seizures (21.5–66.7%) and survival rates (85–100%), highlighting the relevance of these “minor” components. This study highlights the necessity of standardization in pharmaceutical development, and although it could be construed to support the single molecule therapeutic model (Bonn-Miller et al., 2018), it requires emphasis that complex botanicals can meet American FDA standards (Food and Drug Administration, 2015). Specifically, two Cannabis-based drugs have attained regulatory approval, Sativex®(nabiximols, US Adopted Name) in 30 countries, and Epidiolex®in the United States.

The question then arises: Can a Cannabis preparation or single molecule be too pure, thus reducing synergistic potential? Recent data support this as a distinct possibility. Anecdotal information from clinicians utilizing high-CBD Cannabis extracts to treat severe epilepsy, such as Dravet and Lennox-Gastaut syndromes, showed that their patients demonstrated notable improvement in seizure frequency (Goldstein, 2016; Russo, 2017; Sulak et al., 2017) with doses far lower than those reported in formal clinical trials of Epidiolex, a 97% pure CBD preparation with THC removed (Devinsky et al., 2016, 2017, 2018; Thiele et al., 2018). This observation was recently subjected to meta-analysis of 11 studies with 670 patients in aggregate (Pamplona et al., 2018). Those results showed that 71% of patients improved with CBD-predominant Cannabis extracts vs. 36% on purified CBD (p < 0.0001). The response rate at 50% improvement in seizure frequency was not statistically different in the two groups and both groups achieved seizure-free status in about 10% of patients. However, the mean daily doses were markedly divergent in the groups: 27.1 mg/kg/d for purified CBD vs. only 6.1 mg/kg/d. for CBD-rich Cannabis extracts, a dose only 22.5% of that for CBD alone. Furthermore, the incidence of mild and severe adverse events was demonstrably higher in purified CBD vs. high-CBD extract patients (p < 0.0001), a result that the authors attributed to the lower dose utilized, which was achieved in their opinion by the synergistic contributions of other entourage compounds. Such observations support the hypothesis of greater efficacy for Cannabis extracts combining multiple anticonvulsant components, such as CBD, THC, THCA, THCV, CBDV, linalool, and even caryophyllene (Lewis et al., 2018).

These studies and others provide a firm foundation for Cannabis synergy, and support for botanical drug development vs. that of single components (Bonn-Miller et al., 2018), or production via fermentation methods in yeast or other micro-organisms. An example of the power of conventional selective breeding is illustrated (Figure 2), in the form of a Cannabis chemovar named CaryodiolTM for its enhanced caryophyllene content (0.83%) as a CB2 agonist, along with highly favorable Type III THC:CBD ratio of 1:39.4. Such a preparation portends to be applicable to treatment of numerous clinical conditions including: pain, inflammation, fibrotic disorders, addiction, anxiety, depression, autoimmune diseases, dermatological conditions and cancer (Pacher and Mechoulam, 2011; Russo, 2011; Xi et al., 2011; Russo and Marcu, 2017; Lewis et al., 2018). Producing such a combination from microbial sources might require combinations of cannabinoids from multiple yeast species and, as a result, it would represent a combination product subject to a difficult regulatory path compared to Cannabis preparations from extracts of a single species (e.g., nabiximols) that has been accepted as a unitary formulation in 30 countries across the globe (Food and Drug Administration, 2015).
 

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Great article!

Wondering what is the best way to decarboxylize kief? Should it be covered or not?

I cook kief spread evenly in a baking pan for at 240F for 40 minutes.
 

pipeline

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My hope for the future is authorities will be allowing patients to breed using high plant counts to develop phenotypes and strains which have the best enterauge synergy effects to treat their issues. Also the strain would become acclimated to their local climate.

A lot of the strains I have tried are similar and lack some of the enterage effects of my personal cultivar Sativa Candy Chunk which is an F15.
 
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