Who uses Tissue Culturing

[Chimera]

Tissue culture is a whole series of practices, it can be much more complicated than just "cloning".

Meristem culture, callus induction and regeneration, embryogenesis, organogenesis, suspension culture, microspore or anther culture, cryopreservation... I've been playing with TC for about 15 years. For most cultivators there is no benefit to TC as stem cuttings are easy and low-input in terms of resources, labour, etc, but it does provide some benefits in various research paradigms for germplasm storage, etc.

-Chimera

 

[Hammerhead]

Thanks for Chiming in Chimera I was hoping someone with your knowledge would post.. Much appreciated. I ordered the starter kit 60$ but the place I ordered it from sent me the big 220$ kit for 60$ instead? Its much more involved..I have been reading and watching the video's it is a very involved process just to clean even the parts you TC get washed. I will try it at the very least If I know how to use it I can when I find plants I cant clone for what ever reason.

 

[DoubleTripleOG]

Tissue culture is a whole series of practices, it can be much more complicated than just "cloning".

Meristem culture, callus induction and regeneration, embryogenesis, organogenesis, suspension culture, microspore or anther culture, cryopreservation... I've been playing with TC for about 15 years. For most cultivators there is no benefit to TC as stem cuttings are easy and low-input in terms of resources, labour, etc, but it does provide some benefits in various research paradigms for germplasm storage, etc.


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-Chimera

That has to be the smallest complete leaf I have ever seen, lol!

I would definitely try doing tissue cuture sometime. The thought of it reminds me of 10th grade bio class. We were given the little petri dishes pre-loaded agar. Then had to find our own unique source of bacteria to grow in the dish. Mine grew yellow and purple for some weird reason.

From the few things I have read about tissue culture, I understand that it takes quite a while until you actually have a plant. But as a last resort, or for a fun project it sounds great.

Think of the thievery that will happen if TC ever goes mainstream for cannabis. Dudes won't have to steal cuts, they could just rip off a little chunk of leaf, and that's all it will take, lol!

 

[Ollie]

Bomb Info and Pictures, those seeds Agains NICE.

Keep it going

 

[JointOperation]

I was going to try it.. but only was going to do it to get the vigor back in a few clones I was passed.. then a friend of mine. got a kit.. and did it.. so I just grabbed a new cut after he was done..

the vigor was back.. the fucking plant did soooo much better in flower. better yields.. frostier.. so something was totally wrong with her maybe old age.. was supposed to be 10 years old.. clone of clone of clone of clone and so on .

 

[Dready_jake]

Correct its suppose to make perfect copies with no worries of insects or pathogens(assuming you don't have it to begin with). Keeping a healthy stock in a very small space. I will give it a shot. I just don't see how this would replace the normal cloning practices we currently do.

That's pretty Mich exactly how I feel about it

 

[Tonygreen]

there are alot of protocols out there you can find.

 

[Mr. Majestyk]

So its the hood/ glove box that you really need right? Well washed samples in agar, held in low light?

 

[Hammerhead]

Not according to Bill Graham. He is the vendor for these kits. I watched a few of his vid's he was in his kitchen using a tote on its side for his working space.

 

[ronbo51]

I think you would need a hood to have consistent results. I did mushrooms for 7 years and the first piece of equipment I bought was a hood. You need it to do transfers and you can store plates and sterilized equipment in it after you take it out of your pressure vessel which is the second piece you have to buy. I had a large autoclave for bulk sterilizing but also had several large pressure cookers for other things. You need to take stuff out of your pressure vessel directly to cool under the hood. Agar is pretty much sugar and even one bacteria will contaminate your plate and ruin everything. I knew folks who tried the peroxide methods and they never worked, although people still claim it does and perhaps, but you would never find a lab that counted on things going right head down that road. I had a clean room, practiced clean room techniques, did everything i could to keep the filthy world out of my lab but still had random contaminates. To me the biggest advantage of tissue culture would be combined with long term storage so as to insure that your genetic library remains intact and viable. This would allow you to let cuts come and go knowing you could always get it back. Also people could trade cuts much more easily by just mailing plates around.

 

[Backyard Farmer]

I think you need info that isn't neccesarily public to be successful..
I went all out with a laminar flow hood I borrowed from a friend, complete sterile procedure and everything else and with all that a succesful hormone combination/ratio isn't just going to jump out at you..
Im sure with this you may get a few of the links to the Chinese Hemp TC procedures but those don't really work IME..
I got annoyed at proper procedure still resulting in failure, and the lack of instant gratification made me move on to other things..

Over all I would say it's only really useful in life or death situations with plants or commercial applications.
If you need 5000 clones from a plant in 5-9 months id say go for it. Lol

There's papers from UMiss on culturing , organogenesis with TDZ , and encapsulation all available for download for free online.

 

[ronbo51]

for what it's worth, I built my hood. I bought what is called a "drop in" unit made for drop ceilings in clean rooms. They come 2'x2' or 2'x4'. I used a 2x4 unit laid on its side and built a cabinet out of inert pvc sheets and plexiglass. The unit comes pre wired with a matched epoxy fan and is hospital/ clean room quality for maybe less than a grand. The pressure cookers were specially made to form a vacuum so as to not suck in contaminated air as it cooled. The company that made them is called "Mushroom People". They are associated with The Farm in Tennessee and are good folks. A hood and a pressure cooker is what you need.

 

[Gil Tokerson]

I'm no help with the tc, but I have dabbled in mycology, and it is possible to stay sterile without spending a bunch of money on a hood, etc. It would make it easier, but not at all necessary.
I do have one question for anyone that might know:
Someone on page 1 mentioned that you might end up with a sample sitting for a year without doing anything. That is more interesting to me than using tc for making clones. I would love to be able to save a cutting from a plant in a kind of suspended animation, then pull it out and root it a year or 2 later. Like the "clone bank"that someone mentioned. Rooting cuttings isn't that hard, but maintaining mothers, and constantly taking cuttings is a pain. Especially for small time guys like me. It would make the process of sorting through packs of seeds looking for the keepers much easier too. I know everyone has lost "the one" at least once due to not keeping a cut.
This is probably all way of topic, so sorry about that. But if anyone knows, please chime in.

 

[Dready_jake]

I'm no help with the tc, but I have dabbled in mycology, and it is possible to stay sterile without spending a bunch of money on a hood, etc. It would make it easier, but not at all necessary.
I do have one question for anyone that might know:
Someone on page 1 mentioned that you might end up with a sample sitting for a year without doing anything. That is more interesting to me than using tc for making clones. I would love to be able to save a cutting from a plant in a kind of suspended animation, then pull it out and root it a year or 2 later. Like the "clone bank"that someone mentioned. Rooting cuttings isn't that hard, but maintaining mothers, and constantly taking cuttings is a pain. Especially for small time guys like me. It would make the process of sorting through packs of seeds looking for the keepers much easier too. I know everyone has lost "the one" at least once due to not keeping a cut.
This is probably all way of topic, so sorry about that. But if anyone knows, please chime in.

That's a great point. Especially if you are dealing with pest or pm. You could suspend all of your genetics indefinitely and sterilize everything including the genetics in the process.

Now you got me thinking. But I'm broke so simple aero cloning it is still

 

[Hammerhead]

This is really good data.. I want to thank all who participated in this poll.

They do sale a starter kit for 60$. This is what I ordered but they sent the larger 220$ kit. Dont know why. I can only assume they couldn't sale it like my local store. So they sent it to me for 60$ instead of taking a total loss on it ? I'm just guessing. I do know my local store cant sale the 2 they have had for 2 years.

 

[Chimera]

It's one thing to be able to grow and multiply some shoots, another to successfully root them, and even another still to keep plants in jars for long term storage without contamination. Subculturing is required at relative frequencies, and each subculture is an opportunity for contamination.

Most gardeners will have a much better result preserving germplasm as stem cuttings that are heavily pinched and bonzai'd under low light. I keep backups in 36 cell nursery inserts with very little lighting, and they can last well over a year given a proper diet and watering, and regular pruning. This is much easier, less likely to fail, and less labour and resource intensive.

I like your sig BF -what is it they say... that imitation is the most sincere form of flattery?

-Chimera

 

[Backyard Farmer]

Chimera , listen to sizzla album with "throw away the guns" , he throws the line numerous times , what's the saying ... Nothing is original ...

 

[xxxstr8edgexxx]

its totally worth doing in the sense that even if youre not a corporate funded small biotech firm trying to work projects that are beyond the scope of the average grower you can still play with some otherwise unobtainable goals without. its really not that necessary to have 100% success if you're just tinkering.

so commercially it requires a hood because you cant run a deficit that would be easily cured, at home with a side ways tote and some creative techniques you can acheive enough success that you can experiment with the technique and expand if it seems to inspire you. a flow hood that gets the job done isnt hard to construct either.

 

[Highsenburg831]

I want to know who uses Tissue culturing. If you know anyone that uses it. Those that don't use it why. DO you think there a waste of $$$ so not even worth trying ??

i used to work for a berry marketing company with an extensive breeding program and tissue culture lab. the company breeds high end proprietary fruit and contracts with growers around the world to grow the fruit.

the lab produces several million plants per year via TC. but that's just the beginning of the propagation cycle... the plants propagated in the lab are brought to nurseries to grow out. as these mother plants are grown out, cuttings are taken and rooted. and then cuttings off those plants are taken and rooted.

when i started growing (a few years ago) i bought a TC kit and got it to work at home. as i learned more about the agricultural propagation process, i saw that TC for cannabis was kind of a waste of resources for most growers.

where it could make sense would be in the discreet transfer of starts around the world.

as chimera mentioned, it's more efficient to preserve genetics with more conventional methods. and just knowing what i know about the process in both applications - propagation is more efficient with traditional cloning methods too.

just my observations having seen the process in action

 

[floralheart]

For cannabis? @floral

I have about 15 different hormones And PPM (plant preservatives) agar, everything one would need minis a impropto glove box/hood.. If you end up wanting to try it I could prolly send ha what you need if you figure out a ratio..

You shouldn't be spending more than 50-100 if you buy it all new anywho..

Look up PF tek for mushrooms so you get an idea of the procedure then just swap the medium and a few of the steps and there ya go.. Lol @frostbyte

a person i saw on a local board can do about anything with tissue culture... very sharp

then i met another dude at a cannabis club meeting who was deep into it, and we stayed in contact for a while

he ran one of the clubs, and was pushing to get people together to do it and split costs

never tried it, tried mushrooms twice, never was entirely successful - so i tossed them and never retried. My first try, i got about 50/50 contamination of a few jars, using the poor mans method of a rigged up plastic glovebox.