I want to know who uses Tissue culturing. If you know anyone that uses it. Those that don't use it why. DO you think there a waste of $$$ so not even worth trying ??
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Who uses Tissue Culturing
- Thread starter Hammerhead
- Start date
I think you need info that isn't neccesarily public to be successful..
I went all out with a laminar flow hood I borrowed from a friend, complete sterile procedure and everything else and with all that a succesful hormone combination/ratio isn't just going to jump out at you..
Im sure with this you may get a few of the links to the Chinese Hemp TC procedures but those don't really work IME..
I got annoyed at proper procedure still resulting in failure, and the lack of instant gratification made me move on to other things..
Over all I would say it's only really useful in life or death situations with plants or commercial applications.
If you need 5000 clones from a plant in 5-9 months id say go for it. Lol
I went all out with a laminar flow hood I borrowed from a friend, complete sterile procedure and everything else and with all that a succesful hormone combination/ratio isn't just going to jump out at you..
Im sure with this you may get a few of the links to the Chinese Hemp TC procedures but those don't really work IME..
I got annoyed at proper procedure still resulting in failure, and the lack of instant gratification made me move on to other things..
Over all I would say it's only really useful in life or death situations with plants or commercial applications.
If you need 5000 clones from a plant in 5-9 months id say go for it. Lol
The kits I'm familiar with are from Bill Graham.. Didn't even know there was any Chinese knockoffs. In not referring to those. I just want to know from the normal everyday people if they use one for cloning . If any breeders use one would be interesting to know. I don't think any breeder uses 1 of these. At least none I know use 1.
AS for be public ?? I don't know any other way to get a response from thousands quickly...I have a kit but want to know how popular this method really is or is it just a passing oddity that most wont use.
AS for be public ?? I don't know any other way to get a response from thousands quickly...I have a kit but want to know how popular this method really is or is it just a passing oddity that most wont use.
I used kitchen kulture..
Im saying there are cannabis "conglomerates" who very likely know how to do this but most likely haven't released it publicly due to money invested privately er whatever.
Im talking about hormone ratios.. There isn't a "cannabis" kit for TC to my knowledge.. Your using the same materials as anyone else doing TC on any other plant just different chemicals and RATIOS per L of agar and nutrient mix.. Im not talking about some knock off kit im talking about a ratio of hormone/medium that is actually succesful in shooting (creating new growth -leaves) and roots (making it root)
Basically you can buy everything you need but that doesn't mean you'll be succesful.. I've heard the ratios can even be slightly strain specific.. I don't know if that true but just a lil nugget.
You may get it to root, you may only get it to shoot.. Or it may just sit in a sterile medium for a year and do nothing..
Im saying there are cannabis "conglomerates" who very likely know how to do this but most likely haven't released it publicly due to money invested privately er whatever.
Im talking about hormone ratios.. There isn't a "cannabis" kit for TC to my knowledge.. Your using the same materials as anyone else doing TC on any other plant just different chemicals and RATIOS per L of agar and nutrient mix.. Im not talking about some knock off kit im talking about a ratio of hormone/medium that is actually succesful in shooting (creating new growth -leaves) and roots (making it root)
Basically you can buy everything you need but that doesn't mean you'll be succesful.. I've heard the ratios can even be slightly strain specific.. I don't know if that true but just a lil nugget.
You may get it to root, you may only get it to shoot.. Or it may just sit in a sterile medium for a year and do nothing..
I've met two people who use it, over a 7 year period.
I have no Knowledge of this either.. I wanted to see how many use this regularly or is it just to much effort.. I talked t o my Local store they have 2 of the large 220$ kits they cant sale. They said they had them for 2 years
For cannabis? @floral
I have about 15 different hormones And PPM (plant preservatives) agar, everything one would need minis a impropto glove box/hood.. If you end up wanting to try it I could prolly send ha what you need if you figure out a ratio..
You shouldn't be spending more than 50-100 if you buy it all new anywho..
Look up PF tek for mushrooms so you get an idea of the procedure then just swap the medium and a few of the steps and there ya go.. Lol @frostbyte
I have about 15 different hormones And PPM (plant preservatives) agar, everything one would need minis a impropto glove box/hood.. If you end up wanting to try it I could prolly send ha what you need if you figure out a ratio..
You shouldn't be spending more than 50-100 if you buy it all new anywho..
Look up PF tek for mushrooms so you get an idea of the procedure then just swap the medium and a few of the steps and there ya go.. Lol @frostbyte
yes just for cannabis
Dready_jake
Member
I have done very limited research on tissue culture but a small amount. And from what I know forcing a plant to grow from a hand full of cells results in slightly more stress extenuating genetic drift. Now if I can throw a cutting in my homemade aerocloner with nothing more than a scrape and have roots in 6 days with very little risk of gentic drift, why would I take it that much smaller and increase the veg time and risk of genetic drift in My opinion.
I believe that it should maybe only be used in dire situations such as cloning normally is never working or the plant is late flower and its your last chance. But only more research and/or someone telling me I'm wrong and an asshole will tell. Lol
I believe that it should maybe only be used in dire situations such as cloning normally is never working or the plant is late flower and its your last chance. But only more research and/or someone telling me I'm wrong and an asshole will tell. Lol
I thought that was the hole point in tissue culturing no genetic drift. Perfect healthy copies with no drift is what they advertise?.. I don't believe in drift anyway no matter how long one keeps cloning the same cut.
Dready_jake
Member
Then I guess its just a matter of if you want to feel like a scientist. Lol jk. But how long until a tc reaches the point of vegging like an aero clone? Such ad I can have a clone in soil from roughly 7-10 days and its off and vegging. How long for a tc?
I'd like to see someone on IC figure the TC thing out. GrayWolf was on it but I think he got distracted with extracting.
I paid extra at Disneyworld in FLA to tour their hydroponic grow op.
they had a big tissue culture lab there and I asked a tech if what they used would work on cannabis and she said yes.
nothing looked all that high tech about the TC besides the clean room.
the tour was really cool and if you ever go to disney check it out.
https://disneyworld.disney.go.com/events-tours/epcot/behind-the-seeds/
You could grow serious trees in here.
They have every type of tricked out hydro tech on display here.
I paid extra at Disneyworld in FLA to tour their hydroponic grow op.
they had a big tissue culture lab there and I asked a tech if what they used would work on cannabis and she said yes.
nothing looked all that high tech about the TC besides the clean room.
the tour was really cool and if you ever go to disney check it out.
https://disneyworld.disney.go.com/events-tours/epcot/behind-the-seeds/
You could grow serious trees in here.
They have every type of tricked out hydro tech on display here.
OgRastaman
Member
I have read a few books on Tchaikovsky but have not,had the proper time yet to set up my sterile lab space to start my project , from what I understand it will work on cannabis and if done correctly it can bring up UN seen genetic traits or hidden traits from the strain such as different phenos and also brings out stronger faster growing plants but once again I do not have experience with this yet if you already have the kit try it out PF what do you have to loose but a little time but if you can get it to work than it could bring about many good things for you
BLESS!
BLESS!
I watched the vid from Mr.Graham he did not use anything special like flow hood. He used a Tuff tote on its side. Cleaned off all his stuff with bleach and RO. He washed the cuttings in RO with peroxide. It was not a sterile environment. He was in his kitchen. It looks like a lengthy process just to clean the stuff and cuttings. I just wanted to see how much other use this. I didn't think its widely excepted and wanted to see if my thoughts where correct. Some idiot was saying there used all the time for cannabis cloning and all breeders use them if they don't they cant be trusted.. That leaves out all breeders I know. I dont know anyone that uses this to clone with. I got curious so I started a poll.
Dready_jake
Member
Cloning produces perfect genetic copies it doesnt bring out different phenotypes of a strain.
Correct its suppose to make perfect copies with no worries of insects or pathogens(assuming you don't have it to begin with). Keeping a healthy stock in a very small space. I will give it a shot. I just don't see how this would replace the normal cloning practices we currently do.
OgRastaman
Member
/-I got these as torrents off the internet but they are also text books you could get on paper/hard cover some really in depth stuff
1) Plant Biotechnology and Genetics
Principles, techniques, and Applications edited by C. Neal Stewart Jr.
2) Dictionary of Plant Breeding 2nd edition (For all plants) By : Rolf H.J. Schlegel
3) Principles of Plant Genetics and Breeding
(For all plants) by : George Acquaah
4) Plants From Testubes : an introduction to Micropropagation 3rd edition By : Lydiane Kyte and John Kleyn
T.c.is a little,different than clo)ning with t.c. you 're generate a tap root as a seed start does , you can also have more rooted cuttings w/ T.c. in a smaller space than you can with traditional cloning, from what I have read in these books you can take a cut from a unhealthy plant and,use T.c to bring it back to its full vigor and strength this,cut must be from the apical stem I belive
1) Plant Biotechnology and Genetics
Principles, techniques, and Applications edited by C. Neal Stewart Jr.
2) Dictionary of Plant Breeding 2nd edition (For all plants) By : Rolf H.J. Schlegel
3) Principles of Plant Genetics and Breeding
(For all plants) by : George Acquaah
4) Plants From Testubes : an introduction to Micropropagation 3rd edition By : Lydiane Kyte and John Kleyn
T.c.is a little,different than clo)ning with t.c. you 're generate a tap root as a seed start does , you can also have more rooted cuttings w/ T.c. in a smaller space than you can with traditional cloning, from what I have read in these books you can take a cut from a unhealthy plant and,use T.c to bring it back to its full vigor and strength this,cut must be from the apical stem I belive
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I want to see someone start up a business providing tissue culture service along with long term genetic storage and sales.
So you could pay a fee to have your gear backed up in a legal state and also purchase all the clone onlys you always wanted to run, clean and the same forever.
The real thing would be seed to bud testing and backup. You give them a sample at the seedling stage and they test and culture.
You have the chance to flower or not based on sex and early cannabinoid info.
Then they test again your finished bud for all terps and esoteric cannabinoids, maybe DNA too eventually. also they could do pesticide and mold testing.
Now if your finished seed turned into something exceptional in any way they would have it on a shelf and you could order as many clones as you wanted to run.
I think something like this in going to be inevitable if patients continue to get the right to grow and be care givers.
I suspect that "clone-only" grade gear gets popped all the time but not preserved.
In the future things will get much more technical with people finding their ideal plant profiles to fit their personal needs.
Or maybe it will be more like cigarettes with just a few generic blends ala NY's mmj program to be.
Somebody needs to get this together in Denver or NorCal and hire lobbyists to allow interstate mailing of genetic cannabis material.
People are going to have to get used to sharing strains quick because in a few years you will be competing against
the richest corporate growers and their insect killing robots, perfect light systems and molecular extract machines.
They will pop thousands of seeds at a time looking for killer phenos.
We got maybe 5-8 years left of cannabis as we knew it if it keeps getting more legal.
Maybe we get pushed deeper underground. Maybe I smoke too many cookies...
So you could pay a fee to have your gear backed up in a legal state and also purchase all the clone onlys you always wanted to run, clean and the same forever.
The real thing would be seed to bud testing and backup. You give them a sample at the seedling stage and they test and culture.
You have the chance to flower or not based on sex and early cannabinoid info.
Then they test again your finished bud for all terps and esoteric cannabinoids, maybe DNA too eventually. also they could do pesticide and mold testing.
Now if your finished seed turned into something exceptional in any way they would have it on a shelf and you could order as many clones as you wanted to run.
I think something like this in going to be inevitable if patients continue to get the right to grow and be care givers.
I suspect that "clone-only" grade gear gets popped all the time but not preserved.
In the future things will get much more technical with people finding their ideal plant profiles to fit their personal needs.
Or maybe it will be more like cigarettes with just a few generic blends ala NY's mmj program to be.
Somebody needs to get this together in Denver or NorCal and hire lobbyists to allow interstate mailing of genetic cannabis material.
People are going to have to get used to sharing strains quick because in a few years you will be competing against
the richest corporate growers and their insect killing robots, perfect light systems and molecular extract machines.
They will pop thousands of seeds at a time looking for killer phenos.
We got maybe 5-8 years left of cannabis as we knew it if it keeps getting more legal.
Maybe we get pushed deeper underground. Maybe I smoke too many cookies...
