NIKT
Active member
This is most likely a polygenic trait. A fairly wide range of variation with the conditions heavily influencing the frequency of appearance, especially those with little male flowers. The technique doesn't really matter at all.
It is selectable, resistance to sex change influenced by the growth conditions. Selection of offspring, multiple, not single screening. This technique was applied to cucumber e.g.
Possibility of obtaining fertile pollen is also "selectable". The text of GW pharma _ there is one sentence on the subject _ relating to the fertility of the CBD line.
The results of crossing monoecious and dioecious forms are known. Industrial varieties _ obtained results show that this is inherited on many genes. Obtained variability of forms in the first generation proves it very clearly. The results vary depending on the selection of components for crossing.
Polygenic inheritance with the norm of genotype response. Growth conditions strongly influence the appearance of male flowers, some plants have a low or no need for any disturbances anymore. [as well as specially bred industrial intersex forms where there are no male ones at all]
Polygenic inheritance, many genes influencing the frequency of showing intersex forms. This fits best with the observed effects. Analogy to cucumber
A similar mechanism, but here with clearly separated sex chromosomes.
Roots ??
https://forum.haszysz.com/threads/th...in-hemp.21202/
rather it is called => The Norm of Reaction .
For example, if plants that easily change sex are selected as a pollen source, this indicates a low reaction threshold. Which, in turn, may be related to the frequency of occurrence of monosexual plants in subsequent generations.
The selection of more difficult sex-changing plants was recommended as a method of selecting plants for feminised lines. Photoperiod torture, first method of development. In the case of cucumber, it is likely that the lowest doses of STS were used to screen the population, at least there are reports of such in the literature.
In the case of, for example, the use of Ga3 on a large number of plants, application of it via the roots, theoretically one would expect selection of plants with a low reaction norm. Ga3 on its own was rather much less effective as a gender changer. Rather large doses with strong side effects. Often at low rates, apart from strong growth, nothing.
Comparative presumptions. This picture, for example, Very high dose Ga3. Very strong effects, including reduction of leaf area and change of leaf structure.
The male flowers did not appear at all. When attaching two : sts and Ga3, male flowers would basically always appear. All forms of silver are much more effective than it, Ga3 does not always produce male flowers, rather high doses only.
I don't know what people do or how, but if they use a technique that works, for example, on 10% of fed plants, they will select pieces that can potentially produce offspring easily showing a male flower without externally giving hormones.
If we are looking for any relationship between the number of intersexes in the obtained offspring and the techniques used. Rather, you would have to look at this: potential selection of plants that may yield more offspring with the trend. When using as a pollen source of plants that change sex easily with a less certain technique, selection of plants with a low reaction norm can be expected. What is embedded in the genes of the plants we change sex, from the mere possibility of obtaining fertile pollen to the frequency of showing intersexes later.
If you choose pollen donors from plants that produce male flowers at higher doses, and are, for example, relatively resistant to sex change after Ga3, in my opinion, in this case, the probability of hermaphords occurrence in subsequent generations is lower. Same selection as for any other trait.
It seems to me that, from a purely practical point of view, the single spray technique is the most convenient. I have descriptions from a seedbank in the Netherlands, they where spray "buckets" in multiple repetitions. Probably very dilute solutions: no concentrations were given. I don't know why so: time-consuming and rather reminiscent of the techniques described for CS.
Methods for Ga3 _ descriptions _ have been available online for 20 years. At least _ their effectiveness was not 100% : with strong side effects, which are basically absent after STS.
Combining these substances makes sense, breaking up dense inflorescences. Potentially useful for seed production under field conditions. Better pollen release. It was tested by someone, I don't know with what effects. In general, some people have been producing feminized seeds not under lamps, but simply in the field.
As already mentioned, the hopes to improve pollen fertility have not come true. Otherwise, no improvement was obtained in the experiment. This does not mean that it will not improve your fertility somewhat otherwise. Only tests, in the case of cucumbers, I think fertility is better under STS, I don't remember, it was searched too long ago. The STS method works not only for parthenocarpic cucumbers and hemp. European hops reacted in the same way. This works for most dioecious plants, sometimes the opposite way in a few cases.
For example, it is impossible to obtain fertile pollen in white sapwood, as the genetic changes between male and female plants have gone too far. [some crazy people were playing with other genres during the tests, sometimes repeating tests from the literature, sometimes not]
Probably some scientific literature on the subject will appear in some time. Time and partial legalization.
The funny thing is that usually people who produced seeds commercially were not willing to share their experiences. And it really turned out that it is simply enough to replace the word cucumber with hemp, and apply all techniques for parthenocarpic cucumbers. Nothing new - just variations on recipes that were available before.
The "high-dose" technique was also available earlier.
The first description on the internet and the report on the use of STS is a former overgrow. Comparison of STS and Ga3, some photos. It is possible that it was 20 years ago? .. maybe it is possible ?? Sometimes a person does not know when and how time passes.
It was suspected that there was a link between too high STS doses and pollen lack of fertility, but this is unlikely to be confirmed.
At the concentration you are using, a single spray should be sufficient to change the sex of each plant. Not always 100%, some female flowers may remain, small amounts may remain intersexual, the seeds S1 from them are usually deformed. Often there are also twins, hearts, etc. That's more or less how it turned out in this dose range. Not entirely repetitive, people used different liquid expenses for spraying, whether the addition of surfactants or not.
Additionally, some varieties are a bit more reluctant than others. But a single 3 mM in my opinion is enough.
It's been a lot of years so I'm not 100% sure anymore. Time and it is a bit blurry, and it can even add false memories.
All that I am writing is more of an impression and my own interpretation. I can't prove it in any way. It seems to me that this is the case, somewhat from my own experience, a lot from the results of others, and a lot from the work of another plant, where breeders have created purely female lines that are highly resistant to external factors that might cause male flowers to emerge.
The cucumber data most closely matched the observed results for cannabis. Similar influence of developmental conditions on the appearance of male flowers, essentially identically acting sex-altering chemicals. A slightly different pattern of sex inheritance - no distinct chromosomes, the Y chromosome is distinctly different in cannabis.
I don't know about the negative influence of repeated high dose STS spraying on pollen fertility. Tests like clone and several techniques were not performed. Tests, a few plants, and the same procedure yes.
Rather, it seemed to me that whether the pollen is fertile or not depends on the plant, and not on the technique. One of the varieties did not create pollen at all regardless of what you did. The second is no problem also no matter what you do. I haven't checked dozens of varieties myself, so I don't know if it's a coincidence or ??
Uncertainty is something that always stays even after some tests. You never get an exact picture at a small scale. I guess ??
Photo, effect of combining Ga3 with silver nitrate. Ga3 later given to change the shape of the inflorescences to a stretched one similar to the real male. Not only was it suspected that this might improve fertility but also facilitate pollen separation. [ male flowers do not always open, it is described in the article which is linked here at the beginning _ and also in the thread on F #, some experimenters dried and crushed them ]
There is also info in the thread. on long-term storage of the obtained dried pollen. Freezing. Maybe not directly in the thread, but in one of the derivatives. A technique similar to that once shown at OG, where desiccators were used, with a desiccant and refrigeration for storage.
The descriptions of the stability of STS solutions stored in the dark, found in the scientific literature, also confirmed exactly. Poorly stored solutions decompose partially, giving off silver sulphide. But even they were still quite effective after more than a year of storage.
I don't remember any more at the moment.
Sorry for the language. Writing in this language is too difficult for me. Unfortunately, AI help gives poor results.
It is selectable, resistance to sex change influenced by the growth conditions. Selection of offspring, multiple, not single screening. This technique was applied to cucumber e.g.
Possibility of obtaining fertile pollen is also "selectable". The text of GW pharma _ there is one sentence on the subject _ relating to the fertility of the CBD line.
The results of crossing monoecious and dioecious forms are known. Industrial varieties _ obtained results show that this is inherited on many genes. Obtained variability of forms in the first generation proves it very clearly. The results vary depending on the selection of components for crossing.
Polygenic inheritance with the norm of genotype response. Growth conditions strongly influence the appearance of male flowers, some plants have a low or no need for any disturbances anymore. [as well as specially bred industrial intersex forms where there are no male ones at all]
Polygenic inheritance, many genes influencing the frequency of showing intersex forms. This fits best with the observed effects. Analogy to cucumber
A similar mechanism, but here with clearly separated sex chromosomes.Roots ??
https://forum.haszysz.com/threads/th...in-hemp.21202/
rather it is called => The Norm of Reaction .
For example, if plants that easily change sex are selected as a pollen source, this indicates a low reaction threshold. Which, in turn, may be related to the frequency of occurrence of monosexual plants in subsequent generations.
The selection of more difficult sex-changing plants was recommended as a method of selecting plants for feminised lines. Photoperiod torture, first method of development. In the case of cucumber, it is likely that the lowest doses of STS were used to screen the population, at least there are reports of such in the literature.
In the case of, for example, the use of Ga3 on a large number of plants, application of it via the roots, theoretically one would expect selection of plants with a low reaction norm. Ga3 on its own was rather much less effective as a gender changer. Rather large doses with strong side effects. Often at low rates, apart from strong growth, nothing.
Comparative presumptions. This picture, for example, Very high dose Ga3. Very strong effects, including reduction of leaf area and change of leaf structure.
The male flowers did not appear at all. When attaching two : sts and Ga3, male flowers would basically always appear. All forms of silver are much more effective than it, Ga3 does not always produce male flowers, rather high doses only.
I don't know what people do or how, but if they use a technique that works, for example, on 10% of fed plants, they will select pieces that can potentially produce offspring easily showing a male flower without externally giving hormones.
If we are looking for any relationship between the number of intersexes in the obtained offspring and the techniques used. Rather, you would have to look at this: potential selection of plants that may yield more offspring with the trend. When using as a pollen source of plants that change sex easily with a less certain technique, selection of plants with a low reaction norm can be expected. What is embedded in the genes of the plants we change sex, from the mere possibility of obtaining fertile pollen to the frequency of showing intersexes later.
If you choose pollen donors from plants that produce male flowers at higher doses, and are, for example, relatively resistant to sex change after Ga3, in my opinion, in this case, the probability of hermaphords occurrence in subsequent generations is lower. Same selection as for any other trait.
It seems to me that, from a purely practical point of view, the single spray technique is the most convenient. I have descriptions from a seedbank in the Netherlands, they where spray "buckets" in multiple repetitions. Probably very dilute solutions: no concentrations were given. I don't know why so: time-consuming and rather reminiscent of the techniques described for CS.
Methods for Ga3 _ descriptions _ have been available online for 20 years. At least _ their effectiveness was not 100% : with strong side effects, which are basically absent after STS.
Combining these substances makes sense, breaking up dense inflorescences. Potentially useful for seed production under field conditions. Better pollen release. It was tested by someone, I don't know with what effects. In general, some people have been producing feminized seeds not under lamps, but simply in the field.
As already mentioned, the hopes to improve pollen fertility have not come true. Otherwise, no improvement was obtained in the experiment. This does not mean that it will not improve your fertility somewhat otherwise. Only tests, in the case of cucumbers, I think fertility is better under STS, I don't remember, it was searched too long ago. The STS method works not only for parthenocarpic cucumbers and hemp. European hops reacted in the same way. This works for most dioecious plants, sometimes the opposite way in a few cases.
For example, it is impossible to obtain fertile pollen in white sapwood, as the genetic changes between male and female plants have gone too far. [some crazy people were playing with other genres during the tests, sometimes repeating tests from the literature, sometimes not]
Probably some scientific literature on the subject will appear in some time. Time and partial legalization.
The funny thing is that usually people who produced seeds commercially were not willing to share their experiences. And it really turned out that it is simply enough to replace the word cucumber with hemp, and apply all techniques for parthenocarpic cucumbers. Nothing new - just variations on recipes that were available before.
The "high-dose" technique was also available earlier.
The first description on the internet and the report on the use of STS is a former overgrow. Comparison of STS and Ga3, some photos. It is possible that it was 20 years ago? .. maybe it is possible ?? Sometimes a person does not know when and how time passes.
It was suspected that there was a link between too high STS doses and pollen lack of fertility, but this is unlikely to be confirmed.
At the concentration you are using, a single spray should be sufficient to change the sex of each plant. Not always 100%, some female flowers may remain, small amounts may remain intersexual, the seeds S1 from them are usually deformed. Often there are also twins, hearts, etc. That's more or less how it turned out in this dose range. Not entirely repetitive, people used different liquid expenses for spraying, whether the addition of surfactants or not.
Additionally, some varieties are a bit more reluctant than others. But a single 3 mM in my opinion is enough.
It's been a lot of years so I'm not 100% sure anymore. Time and it is a bit blurry, and it can even add false memories.
All that I am writing is more of an impression and my own interpretation. I can't prove it in any way. It seems to me that this is the case, somewhat from my own experience, a lot from the results of others, and a lot from the work of another plant, where breeders have created purely female lines that are highly resistant to external factors that might cause male flowers to emerge.
The cucumber data most closely matched the observed results for cannabis. Similar influence of developmental conditions on the appearance of male flowers, essentially identically acting sex-altering chemicals. A slightly different pattern of sex inheritance - no distinct chromosomes, the Y chromosome is distinctly different in cannabis.
I don't know about the negative influence of repeated high dose STS spraying on pollen fertility. Tests like clone and several techniques were not performed. Tests, a few plants, and the same procedure yes.
Rather, it seemed to me that whether the pollen is fertile or not depends on the plant, and not on the technique. One of the varieties did not create pollen at all regardless of what you did. The second is no problem also no matter what you do. I haven't checked dozens of varieties myself, so I don't know if it's a coincidence or ??
Uncertainty is something that always stays even after some tests. You never get an exact picture at a small scale. I guess ??
Photo, effect of combining Ga3 with silver nitrate. Ga3 later given to change the shape of the inflorescences to a stretched one similar to the real male. Not only was it suspected that this might improve fertility but also facilitate pollen separation. [ male flowers do not always open, it is described in the article which is linked here at the beginning _ and also in the thread on F #, some experimenters dried and crushed them ]
There is also info in the thread. on long-term storage of the obtained dried pollen. Freezing. Maybe not directly in the thread, but in one of the derivatives. A technique similar to that once shown at OG, where desiccators were used, with a desiccant and refrigeration for storage.
The descriptions of the stability of STS solutions stored in the dark, found in the scientific literature, also confirmed exactly. Poorly stored solutions decompose partially, giving off silver sulphide. But even they were still quite effective after more than a year of storage.
I don't remember any more at the moment.
Sorry for the language. Writing in this language is too difficult for me. Unfortunately, AI help gives poor results.
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