When you created s2 did you use s1 sister plants or self a selected s1? Same for s3?I haven't noticed. Most of the s1, and s2 seeds were fine. The S3 seeds are not made yet so I can't say until I grow some. The s1 and s2 plants had the same variegation as the clone. I had/have two s2 that had this and seemed to have less vigor than the siblings. The 1st was tossed. I'm not sure if the 2nd will be flowered. Most are fine. I think the selections made have the biggest impact. Some would keep the slower variegated phenos. I think those are poor choices to use for breeding.
I had been thinking about this in regards to f2ing a fem hybrid a la open pollination style to preserve genetic diversity…
-DD