Raistlin's Room '08

[Raistlin Majere]

Endo i found THIS and i really like it
i think thats the method i am going to use
only thing is i have no pressure cooker
i always steamed on my stove........................
think i am gonna register over there and have a talk with some of the people

you got any links you can provide for me bro ?
@ shroomery i could not find anything put simple enough for me to understand

 

[Endo]

absolutly brother.. first register over at www.mycotopia.net
2ndly. that tek looks good but a little more complicated than whats nessesary.

and you dont NEED a pressure cooker but it helps..
you can steam your LC just like you would your pints. actually less time is involved.. you can boil it for like 30 min and be done.
the earplugs part is a good idea.. ive never done it. nver needed to really but its a good idea.
also the potatoe is no needed.. just simple white karo syrup and water. will be sufficent.
sterilize and inject with spores and in one week.. you should be ready to shoot up 100's of jars.

also its a good idea to put a few pieces of broked glass in the bottom of the LC jar. it helps when you shake it to break up the clumps of mycilium.

once your good over at mycotopia... check out their archive section, theres so much good info and its organized awesomely. anything you need you can find there.
good luck..
Endo

 

[jipedestran]

Hi Ya Raist.

Ain't been around in a while.

Hope all is well with you and yours.

peace
jip

 

[Raistlin Majere]

endo does this sound right
using a 1/2 pint jar (steralized)glass inside
gonna do the ear plug in the top idea, it seems to work quite well for people
1 and 1/3 cup water (distilled)
add 3-4 Teaspoons of light karo syrup to the water and stir until mixed very well
steam on stove for 20-30 min, a question on this. should the lid be loose like when making cakes ?
inject 2-3 cc's of spore solution

incubate at 86F, how many days/weeks ?

seems easy i think, if this is what to do
my brain hurts from searching/reading

jipedestran ~ good 2 see ya again bro !

 

[Raistlin Majere]

ok guys one more post for the night, actually till sunday night probably
gonna cozy up with the wife a bit this weekend, she's been looking lonely and dropping hints
so its all chick flicks and foot rubs for me probably

anyhow 3 of my 4 PH cuts now have roots
sorry no pix but i'll get them up then
the 4th one got a rotted stem ?
i took these cuts about a week or so into flower

one of my AK showed male preflowers so i axed him, and pulled his two cuts
leaving me with 3 open spots in the EZ
we cannot have that so i took cuts from my runt CC and filled them up
now i have cuts for all the CC in there

pictures on sunday evening if i can, monday at the latest
and now i am finally going crazy with no smoke
quit the ciggs for 3 days now, thats good
but i got some nice bud formations going on with the PH, thats at only 25 daze flower i think
gonna be a rough next couple of weeks for this hound doggy

---------------------------
peace to you all this weekend, i see you sunday evening :friends:

 

[chubbynugs]

OMG it is so hard keeping up with things in your thread! LOL. Great how to on the muckers. My girl hasnt gave me the for sure go ahead yet on those. I think she wants my outdoor grow to finish first.

 

[vintner]

Hey Raistlin: Congrats on quiting smoking. I wish my wife would. Hang in there Bro. Eat some shrooms and try not to think about it. Have a great weekend Friend and pamper that little lady some! I'm taking mine golfing today, then to a wedding tomorrow. See you Monday!

 

[Endo]

endo does this sound right
using a 1/2 pint jar (steralized)glass inside
gonna do the ear plug in the top idea, it seems to work quite well for people
1 and 1/3 cup water (distilled)
add 3-4 Teaspoons of light karo syrup to the water and stir until mixed very well
steam on stove for 20-30 min, a question on this. should the lid be loose like when making cakes ?
inject 2-3 cc's of spore solution

incubate at 86F, how many days/weeks ?

seems easy i think, if this is what to do
my brain hurts from searching/reading

jipedestran ~ good 2 see ya again bro !

you seems to have it very close to right. i would cut the amt of karo in half, myself, i only use one heapin, teaspoon per 1 pint jar. and fill the pint jar up 3/4 of the way. seems like your recipe has alot of karo in a smaller amt of water... try it both ways.. see which works. but everything else seems correct, and yes leave the lids on like you would for making cakes. you can even cover the top in tinfoil to stop anything from getting in. after steaming let cool, and then innoculate with spores. incubate for at least a week or 10 days. you should see what resembles little floaty sperms. (excuse the term) those are your mycelium. shake them every once and a while to break them up, during the 10 days.

once you feel there is enough in there, shake it up again, and then draw into syringes and innoculate as usual using 1ml per hole. thats pretty much it. your pretty spot on with your tek. good research. when you do it though keep an eye on the karo, if it gets too hot it will turn a golden color, and that may not work so well because of the carmelization. let me know how it goes.
on another note. just got my delivery of syringes.. 7 of them for 50 bucks. good deal. gonna run some Golden Teachers next time, i hear they are very very nice.

Later
Endo

 

[accessndx]

I just wanted to add my experience and 2 cents here pertaining to the amount of fluid used from the syringes relative to the jars:
I was confused about this subject also...I so kind of played it by ear initially. Here's the simple fact: if you have as few as 2 spores innoculating your substrate/medium...that will be enough to sexually reproduce and create mycelium which will then asexually reproduce and "colonize" your medium. It will just take MUCH MUCH longer than adding more spores. Additionally, the faster the mycelium colonize your substrate forming a cake, the less opportunity for "opportunistic" organisms to develop such as virus, mold and bacteria (hence less contaminants). 1ml is perfectly fine for an entire jar of any size IMO. I've done it both ways: putting 1ml in each hole....and 1ml total for 1-4 holes. The concept of putting 1ml in each hole of the jar top is great if you want fast expeditious growth. If you want to stretch your syringes out, you can be a bit on the skimpy side...and it will still grow and develop....just alot slower as I indicated.
I have no knowledge about the "karo" mentioned earlier. I'll have to look into that...anything that can multiply the spores would be great.

Raist: it occurred to me that it may be possible to make crosses between two different mushroom species by innoculating with two different syringes. When people want to cross two strains of marijuana...it's done sexually by taking pollen from one male and hitting the female flowers with it. Then you get a cross between the two. However, since mushrooms reproduce both sexually and asexually.....it may be possible to combine the spores from one and the spores from another strain to make sexual crosses that we haven't tried before. I wouldn't mind taking that penis envy and mixing it with golden teacher, psylocibe atlanteans or mexicana or other strains. Could be kind of "mind blowing". Next time I run mushies, I'm gonna give it a a whirl....couldn't hurt and could be rather fascinating. You may be in a better position to try it right now.....would have to take place at the innoculation stage however...

Gotta recover from my escapades: NIN was awesome last night. I changed my mind about mushies and brought some...the combination of mushies, champagne, and killer smoke was a bit much.....I have some rather blurry pictures that actually looked "good" to me at the time. In the clarity of morning however...ooooooooofah.
We also lucked the hell out: we got to Mohegan sun real late....so they had sold out the rooms....and they upgraded us to a Sky Suite. Those rooms start at 1K/evening....all I can say is "holy crap". We were overlooking the Thames lake/river in CT....whole different energetic vibe up there. The casino I could leave or take....but I wouldn't mind living up there...I can see why the american indians would gravitate and have lived in that region of the country. It's just majestic looking and has that sense of tangible power that eminates from those areas people consider sacred. Nice place to trip man, really nice place.

 

[jipedestran]

Nice trippin balls, hope you won some loot.

not me, my wifey was away last week and i had to feed my jones. got cranked for a good bit of $$$ on the blackjack and dice tables.

Raist, I just read the part about you quitting cigs. Dude, you know that is so awesome. just be strong everyday, and all those times that you would be smoking a cigarette, just think, I can juggle or I can count sheep or I can just sit and watch tv or drive and I just don't need that cigarette.


as for your herbs, if you have trouble the offer I made a while back via pm still stands. You have made it this far, I am confident you will be ok.

Yeah No Tobacco.

peace
jip

 

[Endo]

I just wanted to add my experience and 2 cents here pertaining to the amount of fluid used from the syringes relative to the jars:
I was confused about this subject also...I so kind of played it by ear initially. Here's the simple fact: if you have as few as 2 spores innoculating your substrate/medium...that will be enough to sexually reproduce and create mycelium which will then asexually reproduce and "colonize" your medium. It will just take MUCH MUCH longer than adding more spores. Additionally, the faster the mycelium colonize your substrate forming a cake, the less opportunity for "opportunistic" organisms to develop such as virus, mold and bacteria (hence less contaminants). 1ml is perfectly fine for an entire jar of any size IMO. I've done it both ways: putting 1ml in each hole....and 1ml total for 1-4 holes. The concept of putting 1ml in each hole of the jar top is great if you want fast expeditious growth. If you want to stretch your syringes out, you can be a bit on the skimpy side...and it will still grow and develop....just alot slower as I indicated.
I have no knowledge about the "karo" mentioned earlier. I'll have to look into that...anything that can multiply the spores would be great.

im 99 percent sure it doesnt work that way with muchrooms.. basically from my readings all you will get is half a cake with one strain and half a cake with another strain.. no combining.. if they even survive.. there would be a noticeable deadzone between the 2. ill do a bit of search, for some concrete info.. but to my knowledge, it just dont work that way.

 

[accessndx]

im 99 percent sure it doesnt work that way with muchrooms.. basically from my readings all you will get is half a cake with one strain and half a cake with another strain.. no combining.. if they even survive.. there would be a noticeable deadzone between the 2. ill do a bit of search, for some concrete info.. but to my knowledge, it just dont work that way.

Endo: LOL. Apparently you quoted all the OTHER stuff and responded to the suggestion about mixing two strains. It's my understanding that spores germinate like other "seeds"....but two germinated spores must come together and exchange genetic material that creates mycelium through sexual reproduction. The rest of the mycelium that develops is created through asexual reproduction. Now....please consider the following fact: my brain is completely and totally addled by NO2, mushrooms....weed and other assorted goodies. Sometimes I don't have enough brain cells left to maintain respiration, balance, speech and cognition at the same time....
I don't remember WHERE I got this information.....but unless I have a completely implanted/false memory...I don't know why I'd have that level of detail in my brain at all. I usually forget the stuff I don't need to know.
Since we've been talking mushies, the stoned thought occurred to me "hey...if it develops this way....maybe we can mix things up and make a new strain". Please DO some research....and get a concrete answer. I'd like to know if I just made something up out of thin air or if it's something substantial. If I'm right however...that would be rather cool.

 

[YwouldntI]

-Y-sits with lost expression on face
im sure those posts will come in handy in a couple weeks and i'll even know what some of the terms are LOL.peace-Y-

 

[accessndx]

OK: Here's a brainfull....

Reproduction
Reproduction of fungi is complex, reflecting the heterogeneity in lifestyles and genetic make up within this group of organisms. Many fungi reproduce either sexually or asexually, depending on conditions in the environment. These conditions trigger genetically determined developmental programs leading to the expression of specialized structures for sexual or asexual reproduction. These structures aid both reproduction and efficient dissemination of spores or spore-containing propagules.

Asexual reproduction
Asexual reproduction via vegetative spores or through mycelial fragmentation is common in many fungal species and allows more rapid dispersal than sexual reproduction. In the case of the "Fungi imperfecti" or Deuteromycota, which lack a sexual cycle, it is the only means of propagation. Asexual spores, upon germination, may found a population that is clonal to the population from which the spore originated, and thus colonize new environments.

Sexual reproduction
Sexual reproduction with meiosis exists in all fungal phyla, except the Deuteromycota. It differs in many aspects from sexual reproduction in animals or plants. Many differences also exist between fungal groups and have been used to discriminate fungal clades and species based on morphological differences in sexual structures and reproductive strategies. Experimental crosses between fungal isolates can also be used to identify species based on biological species concepts. The major fungal clades have initially been delineated based on the morphology of their sexual structures and spores; for example, the spore-containing structures, asci and basidia, can be used in the identification of ascomycetes and basidiomycetes, respectively. Many fungal species have elaborate vegetative incompatibility systems that allow mating only between individuals of opposite mating type, while others can mate and sexually reproduce with any other individual or itself. Species of the former mating system are called heterothallic, and of the latter homothallic.

Most fungi have both a haploid and diploid stage in their life cycles. In all sexually reproducing fungi, compatible individuals combine by cell fusion of vegetative hyphae by anastomosis, required for the initiation of the sexual cycle. Ascomycetes and basidiomycetes go through a dikaryotic stage, in which the nuclei inherited from the two parents do not fuse immediately after cell fusion, but remain separate in the hyphal cells (see heterokaryosis).

In ascomycetes, dikaryotic hyphae of the hymenium form a characteristic hook at the hyphal septum. During cell division formation of the hook ensures proper distribution of the newly divided nuclei into the apical and basal hyphal compartments. An ascus (plural asci) is then formed, in which karyogamy (nuclear fusion) occurs. These asci are embedded in an ascocarp, or fruiting body, of the fungus. Karyogamy in the asci is followed immediately by meiosis and the production of ascospores. The ascospores are disseminated and germinate and may form a new haploid mycelium.

Sexual reproduction in basidiomycetes is similar to that of the ascomycetes. Compatible haploid hyphae fuse to produce a dikaryotic mycelium. However, the dikaryotic phase is more extensive in the basidiomycetes, in many cases also present in the vegetatively growing mycelium. A specialized anatomical structure, called a clamp connection, is formed at each hyphal septum. As with the structurally similar hook in the ascomycetes, formation of the clamp connection in the basidiomycetes is required for controlled transfer of nuclei during cell division, to maintain the dikaryotic stage with two genetically different nuclei in each hyphal compartment. A basidiocarp is formed in which club-like structures known as basidia generate haploid basidiospores after karyogamy and meiosis. The most commonly known basidiocarps are mushrooms, but they may also take many other forms (see Morphology section).

In zygomycetes, haploid hyphae of two individuals fuse, forming a zygote, which develops into a zygospore. When the zygospore germinates, it quickly undergoes meiosis, generating new haploid hyphae, which in turn may form asexual sporangiospores. These sporangiospores are means of rapid dispersal of the fungus and germinate into new genetically identical haploid fungal colonies, able to mate and undergo another sexual cycle followed by the generation of new zygospores, thus completing the lifecycle.

Spore dispersal
Both asexual and sexual spores or sporangiospores of many fungal species are actively dispersed by forcible ejection from their reproductive structures. This ejection ensures exit of the spores from the reproductive structures as well as travelling through the air over long distances. Many fungi thereby possess specialized mechanical and physiological mechanisms as well as spore-surface structures, such as hydrophobins, for spore ejection. These mechanisms include, for example, forcible discharge of ascospores enabled by the structure of the ascus and accumulation of osmolytes in the fluids of the ascus that lead to explosive discharge of the ascospores into the air. The forcible discharge of single spores termed ballistospores involves formation of a small drop of water (Buller's drop), which upon contact with the spore leads to its projectile release with an initial acceleration of more than 10,000 g. Other fungi rely on alternative mechanisms for spore release, such as external mechanical forces, exemplified by puffballs. Attracting insects, such as flies, to fruiting structures, by virtue of their having lively colours and a putrid odour, for dispersal of fungal spores is yet another strategy, most prominently used by the stinkhorns.

Other sexual processes
Besides regular sexual reproduction with meiosis, some fungal species may exchange genetic material via parasexual processes, initiated by anastomosis between hyphae and plasmogamy of fungal cells. The frequency and relative importance of parasexual events is unclear and may be lower than other sexual processes. However, it is known to play a role in intraspecific hybridization and is also likely required for hybridization between fungal species, which has been associated with major events in fungal evolution.

 

[Endo]

i read it but understand 0 of it.. so are you right or am i right? i understand that you have details in your head and im the same way as you.. cause the first thing i thought was hey lets mix the syringes.. then i read something about it not really working.. so i forgot about it and filed it under DO NOT TRY in my head. im gonna go look real quick..

admitedly this is some half assed searching on my part.. and honestly im just to damn lazy. but i found a few links on mycotopia.net asking the same ? and the simple answer seems to be no.. but there is a slight possiblilty they can hybridize... that being said.. i hope you have a nice lab set up, so you can actually tell the diffrence..
link 1
link 2
link3
link 4

the gist i get is that its POSSIBLE, yet not simple like with cannibus. they will mate and create hybrids, but it seems like its kinda going back to the creation of life on earth. like temp,timing and dinosaur farts must be in the right proportions for it to happen. good luch with that though.. your better off growing them separatly then eating them together. anyways.. if anything i said is wrong please correct me. im still a bit of a noob.

Endo
You must spread some Reputation around before giving it to accessndx again.

 

[accessndx]

I think it's not dissimilar from when you breed together animals from the Equidae family to produce something like a "mule". A mule is the offspring of a jack (male) donkey and a mare (female horse). The much rarer successful mating of a male horse and a female donkey produces a hinny.
I think the question that's raised here is how much of the "parasexual" processes which are not well defined or understood are occurring with potential mixes. Apparently the hyphae or little white "tentacles" that develop from the germinated spore go out in search of food to digest....and sometimes meet up and mix with other hyphae. The question is how compatible are two different hyphae. The answer seems equivocal right now without more research on my part....I know it's POSSIBLE, but how often this occurs is difficult to say. It's without doubt however that if they DO combine...and form a fruiting body (ie-mushroom), the fruiting body will be a combination of the genetics of both hyphae. The body can then develop more fully and produce its' own spores which would be a genetic hybridization of the two genetic lines. The spores would in fact be a new strain.
Bottom line is I need to do a bit of deeper research.

 

[Endo]

id like to change my previous statement, from cannot to "possible but unlikey and difficult to tell. " you sound actually pretty spot on with what you are saying. your correct its not impossible. id rather just grow a bunch of diffrent strains and call it a day. if it was as easy as mating white widow with northern lights.. everyone would have their own hybrid... but its not easy, probably why people dont really do it.
stop messin with nature people!

thought the hinny actually looks like a great workhorse/pet.

 

[cellardweller]

Hey Rip..er..umm Rais..
sorry I forgot whose thread I was in!
just wonderin how's things?
I know you wont be back till tomorrow or Monday, but that's ok . I can wait!

 

[sniffs]

Well Hello Raist !

I've been reading through alot of the pages of this thread and I must say it's a great one ! Keep up the good work my man and i'll be sticking around for the rest of it!

Sniffs

 

[YwouldntI]

wattup raist.i d/l 'd that video access was linkin up it explains alot of stuff im about to pop it on again...look for me to be leanin on you pros whenever i order the strynges.
enjoy the weekend yo.peace-Y-