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question for sam the skunkman on the original haze

LostTribe

Well-known member
Premium user
I havent smoked in 3 weeks and having a hard time deciding which flavor to try first....The Mint it shall be and I will go ahead and offer an apology just in case I get a little raving mad shortly. Here we go!

edit: I didnt want to chance having a less than spectacular time so decided to wait until tomorrow. Last time I smoked a whole joint of it I swore I was done smoking and that was with smoking haze on a daily basis for a few months.
 

harvestreaper

Well-known member
Veteran
20220420_175651.jpg

first to show sex a male from macs selection of thh
 

funkyhorse

Well-known member
I am absolutely clueless why this year my haze males dont go intersex like last year
They are having the same treatment, they sexed outdoors and grow indoors, the only difference is they are in 1,3 liter pots instead of 5 liters
Any clue why this happens would be very helpful
Smoh BX
Seedsman Haze july pheno 1 x (Seedsman Haze x Ohz90)
Smoh bx.jpeg


have a nice weekend everybody and enjoy your hazes
 

Goodherb

Well-known member
I am absolutely clueless why this year my haze males dont go intersex like last year
They are having the same treatment, they sexed outdoors and grow indoors, the only difference is they are in 1,3 liter pots instead of 5 liters
Any clue why this happens would be very helpful
Smoh BX
Seedsman Haze july pheno 1 x (Seedsman Haze x Ohz90)
View attachment 18125730

have a nice weekend everybody and enjoy your hazes
Funkyhorse,the backdrop and the geometric sequence of the plant ,make for a lovely artwork.

Positive vibration ,all the way .
 

Goodherb

Well-known member

JohnnyChicago

Well-known member
View attachment 18120090 It was labeled purple haze v2 I believe.
I would like to know the pedigree too.
JohnnyChicago

The exact pedigree of "Purple Haze #12" or Multi Haze = "Purple (OH90 x THH)" x male#12 (ACE Purple Haze *green x Seedsman OH *incense)

To clear up what the #12 does mean.


v2 is only the new version of Pan-o-Haze, that I have made with the Haze#12 male. Could be called Pan-o-Haze #12 too. But it needs to be distinguished from the first version as it is different. Therefore v2.
Will also bring you to a different destination than the v1 does. :)

Here two Purple Haze #12 plants that according to the growers Instagram account have been sold in a dispensary. Haven't tested it myelf yet and this is the only grow of it I have seen.

multihaze (2).jpg
 

Sub24ox7

Well-known member
Tom hill haze f2
Males
And three females
 

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funkyhorse

Well-known member
Ohz the next generation
These girls are 5 and 6 weeks from the day they showed first pistil
All these mutants have been born tri
This phenomena is coming from one of the males of the Seedsman Hz x Ohz90 cross
It seems males are dominant in haze crosses. I had the same phenomena in the progeny of the NL5xH F4 x same boys and I will probably find it in some of the seed made last year
Seedsman Hz BX II This is Seedsman Hz july pheno girl 3 x (Seedsman Hz x Ohz90)
Plant is 2,5 meters tall
Smoh bx 2.jpeg
Smoh bx 2 (2).jpeg

Seedsman Haze X ohz90 girl 9 F2
She is 2,8 meters tall
Seedsman hz mm fem 9 tri.jpeg
Seedsman hz mm fem 9 tri (2).jpeg

Pachamama Haze F2
Top shot
Pachamama haze f2.jpeg

Lower
Pachamama haze f2 (2).jpeg

This is Pachamama Haze now, she is a cut from the second best girl coming from MadMac's Seedman Hz x Ohz90 cross
This is the first time I see such difference from cut and from seed. If the cut is a couple of months old I didnt experience this difference I am seeing this year. The cuts kept from last year are going much much faster than all the rest, even they showed first pistils at the same time as sisters and daughters
Pachamama haze (7).jpeg
Pachamama haze (8).jpeg

Pachamama Haze 2, second cut from last year
Pachamama haze 2.jpeg
Pachamama haze 2 (2).jpeg

Have a nice week everybody
 

Piff_cat

Well-known member
Ohz the next generation
These girls are 5 and 6 weeks from the day they showed first pistil
All these mutants have been born tri
This phenomena is coming from one of the males of the Seedsman Hz x Ohz90 cross
It seems males are dominant in haze crosses. I had the same phenomena in the progeny of the NL5xH F4 x same boys and I will probably find it in some of the seed made last year
Seedsman Hz BX II This is Seedsman Hz july pheno girl 3 x (Seedsman Hz x Ohz90)
Plant is 2,5 meters tall
View attachment 18711735 View attachment 18711736
Seedsman Haze X ohz90 girl 9 F2
She is 2,8 meters tall
View attachment 18711737 View attachment 18711738
Pachamama Haze F2
Top shot
View attachment 18711739
Lower
View attachment 18711740
This is Pachamama Haze now, she is a cut from the second best girl coming from MadMac's Seedman Hz x Ohz90 cross
This is the first time I see such difference from cut and from seed. If the cut is a couple of months old I didnt experience this difference I am seeing this year. The cuts kept from last year are going much much faster than all the rest, even they showed first pistils at the same time as sisters and daughters
View attachment 18711743 View attachment 18711744
Pachamama Haze 2, second cut from last year
View attachment 18711741 View attachment 18711742
Have a nice week everybody
really nice work! i would take those triploids and hit them with your favorite male. what your seeing is a result of sex chromosome aneuploidy, the same phenomenon that led to ojds freak male from haze a g13 x gsc. when the extra chromosome/part of chromosome involved with the aneuploidy condition resides on the y chromosome it throws off the gene dosage and creates more males. your triploids are the result of additive traits in both plants in pedigree combining gene copy numbers to make a full 3rd set. take a microscope and look at the stomatas on those and a regular plant. you should see larger stomata on the triploids. if you hit them with a diploid it will create a hybrid swarm. a way more extreme f2 pheno spread.
 

djonkoman

Active member
Veteran
really nice work! i would take those triploids and hit them with your favorite male. what your seeing is a result of sex chromosome aneuploidy, the same phenomenon that led to ojds freak male from haze a g13 x gsc. when the extra chromosome/part of chromosome involved with the aneuploidy condition resides on the y chromosome it throws off the gene dosage and creates more males. your triploids are the result of additive traits in both plants in pedigree combining gene copy numbers to make a full 3rd set. take a microscope and look at the stomatas on those and a regular plant. you should see larger stomata on the triploids. if you hit them with a diploid it will create a hybrid swarm. a way more extreme f2 pheno spread.
hi, I don't want to be a dick, but I also have a big passion for science, and so I love spreading the understanding of science, and don't like to see the science misinterpreted.

and I'm strugling a bit how I can say this in a not so dickish way, but what you wrote in this post doesn't really make a lot of sense to me. for sure I think it's an overly certain/confident/rash conclusion (science has a lot of maybe, might, could be, but, if), but some of the things you write here really don't make sense at all to me.
and again, not saying this to be a dick at all, I love how you are interested in the science and read up on it, you clearly do have a passion for learning. but I'd also hate to see other people reading this and spreading it further.

so to break down, my criticism in points:

-you are assuming this male in the picture is triploid, and forming the rest of the reasoning on this. this already creates a weakness in the reasoning, as just proving this male is not triploid would make the whole building come down.

-sex chromosome aneuploidy: again a fast, certain conclusion. you are right that if you would have a triploid plant (that is actually triploid, not just called triploid by some dude), that would suggest indeed (one of) the sex chromosomes is double too. aneuploidy as a term though is used when just 1 chromosome (or part of) is double, like in down syndrome with humans (trisomy chromosome 21). but, not for all chromosomes aneuploids occur, for example in humans I think the trisomy of chromosome 21 is the most common, and then there are 2-3 other aneuploid conditions that occur somewhat regularly. but a lot of aneuploids simply never make it that far, they either abort already as an embryo (or even before) or die soon after. anyway, point for the terminology is that aneuploidy and polyploidy are 2 different things, although both can lead to a higher gene dosage.
afaik there isn't much (or any really) research available on aneuploids in cannabis. afaik, no one has ever proven the existence of sex chromosome aneuploids in cannabis. afaik, we also don't know whether having double Y is even viable in cannabis (I think there's some discussions to be found of people looking for them in selfed males, from what I re,ember the male/female ration reported in those seeds would suggest . double Y is lethal). so, never speak with too much certaintity about such details unless you can back it up with a research paper, because it's very easy to help a new myth into the world if you're not speaking carefully.
speaking about gene dosage and sex chromosomes, this also opens up a whole other rabbit hole btw. there was some talk about this somewhere from tom hill (although I didn't fully agree with what he said). namely, what actually determines the sex expression? is it the lack of a y chromosome/presence of y chromosome, or is it the dosage of the x chromosome? (let's say the y is a complete dummy chromosome, than just it's presence instead of another X would lead to half the gene dosage of a female, XX, plant. thus, sex determination could be decided only by the dosage of X theoretically).
in mammals, there is a mechanism to compensate for the increased X-chromosome dosage in females: one X gets 'locked up', making it inactive (visible under a microscope as barr bodies), so males and females both end up with the same dosage of genes on the X.
but plants don't have barr bodies, and cannabis evolved seperate sexes independently from us mammals. it could be cannabis does have other mechanisms to reduce dosage of the X in females/increase expression of genes on the x chromosome in males. but it's also possible instead dosage IS actually different between the sexes, and is involved in determing the sex expression.

which of these 2 options actually determines the sex would matter for how xxy and xyy plants would behave:
-if the Y is a dummy chromosome and dosage of x matters for sex expression, than xxy would show as a female plant. since the y chromosome would be 'empty' of actual information, having 2 of them would likely not be lethal, as long as it's also accompanied ny at least 1 x.
-if presence/absence of y matters fir sex expression, than xxy would show as a male plant, and there is a greater likelyhood xyy is not viable.

then this sentence:
your triploids are the result of additive traits in both plants in pedigree combining gene copy numbers to make a full 3rd set.
I'm not really sure if you are misinterpreting here, or just the way the sentence is worded that confuses me so that I misinterpret your words.
because, the other way around it does make sense: triploidy would lead to increased gene dosage, and gene dosage of specific genes where the effect of copy number is additive (i.e. one allele/copy gives +, 2 copies of the right allele gives ++) could then indeed lead to the triploid's value going beyond the diploid, if it has 3 copies of the right additive allele.
but the way you write it it sounds to me more that you mean you can create triploids from diploids by using parents that have additive genes/alleles for the same trait?? (I'm assuming you mean additive genes/additive alleles that are additive towards the same trait when you say 'additive traits')

(also, keep in mind copy number doesn't matter for all genes. for example sometimes I still see people speculating about copy number variation for the thc gene. but from the info that is available in research papers, my bet would be that focussing on copy number variation for the thc gene would be a pretty fruitless endeavour. I'd rather go for expression variation for the CBG synthase gene, and maybe if expression can't be taken high enough from just natural variation in the expression level(promoter, regulatory elements), only then look at if increased copy number would work. but still for the CBG synthase gene, not THC synthase gene)

it throws off the gene dosage and creates more males.
while my previous criticism was maybe a bit more about use of terminology (which I think is important in science, but it's also ok to be a bit loose with it sometimes especially in an informal place like here), this really is illogical info.
gene dosage is about what happens in a single plant. with some genes, dosage doesn't matter. with others, it does.
but, regardless of what effect the dosage of a gene has on a trait, it doesn't create non-mendellian inheritance.
the only way you can be right (more copies of sex chromosome=more males) is if double Y is viable, because then you could have a xyy male, assuming proper and equal segregation that would result in 2/3 male offspring.
but seeing as it's not so sure doule y can even live, the other potential sex chromosome aneuploidy (xxy) would actually result in more females with that same logic.

BUT

all of that is meaningless since triploids are mostly sterile. so, you don't even get offspring to look at sex ratio. it also means that if this male doesn't have fertility issues, you can be pretty sure it's not triploid.

which also brings me to this:
if you hit them with a diploid it will create a hybrid swarm. a way more extreme f2 pheno spread.
more likely, you will be left without any seeds, or maybe a bunch of aborted/underdeveloped seeds that won't germ. if you're lucky you'll get that 1 or a few rare viable seeds. not much variation to hunt that way.


(btw, when funkyhorse spoke about 'tri' I interpreted it as him just pointing towards the three fingered leaves on the plant in his picture, not anything related to triploidy, or even the threeway-branching that some people mistakenly call triploidy)
 
Last edited:

funkyhorse

Well-known member
Hi guys
I never liked botany in my life, at high school I copied at the examinations...I simply cant believe I love growing plants and I am discovering this so late in life
All the info you post is much appreciated even if I hardly can follow because of lack of knowledge

I call tri this
haze tri.jpeg
haze tri (2).jpeg

I call this quad, I have no clue of the scientific name. Sadly didnt survive, very difficult to grow and make them into adulthood.
quad haze.jpeg
quad haze (2).jpeg

I had quite a lot of tris. very difficult ones, I had tris with NL5xHz F4 progeny too crossed with same mixed pollen haze males
They are much much slower than normal plants and this is the first time I see the haze tris on flower
I never had a male tri. All of them of the different genetics that made it into adulthood were girls

Edit: I just remembered the name. SamS taught me, I am not good student. Whorled phillotaxy
It happened also from pack with CG72 and Fighting Buddha
Edit 2: I just checked the plants, they made seed. One of them is crossed to Oldtimer Green Haze low productivity male and the bigger girl is crossed with the selected male Smoh BX posted in this thread a few pages back. Pachamama Haze F2 is not pollinated yet. I hope to get a little bit of pollen from my Zamal boy in time for them
 
Last edited:

Piff_cat

Well-known member
hi, I don't want to be a dick, but I also have a big passion for science, and so I love spreading the understanding of science, and don't like to see the science misinterpreted.

and I'm strugling a bit how I can say this in a not so dickish way, but what you wrote in this post doesn't really make a lot of sense to me. for sure I think it's an overly certain/confident/rash conclusion (science has a lot of maybe, might, could be, but, if), but some of the things you write here really don't make sense at all to me.
and again, not saying this to be a dick at all, I love how you are interested in the science and read up on it, you clearly do have a passion for learning. but I'd also hate to see other people reading this and spreading it further.

so to break down, my criticism in points:

-you are assuming this male in the picture is triploid, and forming the rest of the reasoning on this. this already creates a weakness in the reasoning, as just proving this male is not triploid would make the whole building come down.

-sex chromosome aneuploidy: again a fast, certain conclusion. you are right that if you would have a triploid plant (that is actually triploid, not just called triploid by some dude), that would suggest indeed (one of) the sex chromosomes is double too. aneuploidy as a term though is used when just 1 chromosome (or part of) is double, like in down syndrome with humans (trisomy chromosome 21). but, not for all chromosomes aneuploids occur, for example in humans I think the trisomy of chromosome 21 is the most common, and then there are 2-3 other aneuploid conditions that occur somewhat regularly. but a lot of aneuploids simply never make it that far, they either abort already as an embryo (or even before) or die soon after. anyway, point for the terminology is that aneuploidy and polyploidy are 2 different things, although both can lead to a higher gene dosage.
afaik there isn't much (or any really) research available on aneuploids in cannabis. afaik, no one has ever proven the existence of sex chromosome aneuploids in cannabis. afaik, we also don't know whether having double Y is even viable in cannabis (I think there's some discussions to be found of people looking for them in selfed males, from what I re,ember the male/female ration reported in those seeds would suggest . double Y is lethal). so, never speak with too much certaintity about such details unless you can back it up with a research paper, because it's very easy to help a new myth into the world if you're not speaking carefully.
speaking about gene dosage and sex chromosomes, this also opens up a whole other rabbit hole btw. there was some talk about this somewhere from tom hill (although I didn't fully agree with what he said). namely, what actually determines the sex expression? is it the lack of a y chromosome/presence of y chromosome, or is it the dosage of the x chromosome? (let's say the y is a complete dummy chromosome, than just it's presence instead of another X would lead to half the gene dosage of a female, XX, plant. thus, sex determination could be decided only by the dosage of X theoretically).
in mammals, there is a mechanism to compensate for the increased X-chromosome dosage in females: one X gets 'locked up', making it inactive (visible under a microscope as barr bodies), so males and females both end up with the same dosage of genes on the X.
but plants don't have barr bodies, and cannabis evolved seperate sexes independently from us mammals. it could be cannabis does have other mechanisms to reduce dosage of the X in females/increase expression of genes on the x chromosome in males. but it's also possible instead dosage IS actually different between the sexes, and is involved in determing the sex expression.

which of these 2 options actually determines the sex would matter for how xxy and xyy plants would behave:
-if the Y is a dummy chromosome and dosage of x matters for sex expression, than xxy would show as a female plant. since the y chromosome would be 'empty' of actual information, having 2 of them would likely not be lethal, as long as it's also accompanied ny at least 1 x.
-if presence/absence of y matters fir sex expression, than xxy would show as a male plant, and there is a greater likelyhood xyy is not viable.

then this sentence:

I'm not really sure if you are misinterpreting here, or just the way the sentence is worded that confuses me so that I misinterpret your words.
because, the other way around it does make sense: triploidy would lead to increased gene dosage, and gene dosage of specific genes where the effect of copy number is additive (i.e. one allele/copy gives +, 2 copies of the right allele gives ++) could then indeed lead to the triploid's value going beyond the diploid, if it has 3 copies of the right additive allele.
but the way you write it it sounds to me more that you mean you can create triploids from diploids by using parents that have additive genes/alleles for the same trait?? (I'm assuming you mean additive genes/additive alleles that are additive towards the same trait when you say 'additive traits')

(also, keep in mind copy number doesn't matter for all genes. for example sometimes I still see people speculating about copy number variation for the thc gene. but from the info that is available in research papers, my bet would be that focussing on copy number variation for the thc gene would be a pretty fruitless endeavour. I'd rather go for expression variation for the CBG synthase gene, and maybe if expression can't be taken high enough from just natural variation in the expression level(promoter, regulatory elements), only then look at if increased copy number would work. but still for the CBG synthase gene, not THC synthase gene)


while my previous criticism was maybe a bit more about use of terminology (which I think is important in science, but it's also ok to be a bit loose with it sometimes especially in an informal place like here), this really is illogical info.
gene dosage is about what happens in a single plant. with some genes, dosage doesn't matter. with others, it does.
but, regardless of what effect the dosage of a gene has on a trait, it doesn't create non-mendellian inheritance.
the only way you can be right (more copies of sex chromosome=more males) is if double Y is viable, because then you could have a xyy male, assuming proper and equal segregation that would result in 2/3 male offspring.
but seeing as it's not so sure doule y can even live, the other potential sex chromosome aneuploidy (xxy) would actually result in more females with that same logic.

BUT

all of that is meaningless since triploids are mostly sterile. so, you don't even get offspring to look at sex ratio. it also means that if this male doesn't have fertility issues, you can be pretty sure it's not triploid.

which also brings me to this:

more likely, you will be left without any seeds, or maybe a bunch of aborted/underdeveloped seeds that won't germ. if you're lucky you'll get that 1 or a few rare viable seeds. not much variation to hunt that way.


(btw, when funkyhorse spoke about 'tri' I interpreted it as him just pointing towards the three fingered leaves on the plant in his picture, not anything related to triploidy, or even the threeway-branching that some people mistakenly call triploidy)
im not even going to credit this with a response. this is all 100 percent true and i have tons more data to prove it, but honestly i couldnt care less what you think. im just trying to help funkyhorse out but you are no where near as smart as youo think you are.its pretty simple. nevils 69 males are aneuploids
 
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