Mosaic Virus in Cannabis pics

[Buckowens]

Fucking TMV again.... Looks like a pH problem every time and usually is.
i have done a few tests in my garden. All of my varieties and all of the other plants I received from other gardens had some sort of endemic penicillium infection. anyone else see this in cultures from tissue?

 

[raphenilweed]

Im sorry I offended you so much.

Im sorry I offended you so much.

We dont need to see plants,, we need to see elisa test results or something or for cried pete.. That somebody somewhere was at some time successful at purposely infecting cannabis with TMV is not quite equally as useless, but of dubious worth indeed.

I am sorry I offended you so much. I was in error of posting pics of verified TMV in a thread titled "Mosaic Virus in Cannabis pics". I wish I did have the test results. The person in which I got the genetics from had it tested and it was positive for Mosaic Virus. The lab tested it twice even because they thought the first test may have been in error. Another person with the same genetics had it tested as well and confirmed positive.

Next time I have something to contribute to the growing community I will be sure to run it by you first for approval.

 

[Buckowens]

Which mosaic? hemp? tomato? tobacco? cucumber? was the test from a real lab? or was it a rapid test kit?

 

[ExEcutioner]

Fucking TMV again.... Looks like a pH problem every time and usually is.
i have done a few tests in my garden. All of my varieties and all of the other plants I received from other gardens had some sort of endemic penicillium infection. anyone else see this in cultures from tissue?

I like this about pH. ....can u expound on the infection, im curious....

 

[Buckowens]

Not yet.
but soon.

 

[P. Dondrub]

These days, I moonlight in a virology lab as an assistant, and I would like to remark that some of the pictures (certainly not all) in this thread are provocative.

Any discussion of negative or positive 'test results' are limited in meaning without a discussion of the testing method. I appreciate those that say they are using Agdia ImmunoStrips, though I certainly hope that the directions are followed carefully and that the control line is well developed.

Also, did you use symptomatic tissue? When or what to sample can be important with some viruses, though TMV may be more forgiving in this regard.

Those test strips do work well for high titer viruses when used correctly. The fact that titer or virus concentration matters at all should indicate though that symptomatic tissue must be used for them!

In general though, there is such a wide range of symptoms being shown here, some are clearly not viral in origin, while others may certainly be.

Certainly we could be dealing with different viruses. I appreciate the paper that Sam the Skunkman uploaded. Also, McPartland references some of the same papers in his important review book, "Hemp Diseases and Pests."

Because of the different symptoms that people show here, as well as very poor nature of the state of research into Cannabis virology, the question here is open. I can run ELISA on a sample, but the question is, what am I testing for? I can only include relatively specific antibodies in my ELISA plate. Hypothetically, I could test for TMV with ELISA, but I personally think that testing for that alone means very little unless we get lucky with a positive result.

Whatever I would do, it better be worth it. Most of these tests require expensive equipment to perform. Antibodies of rare viruses are highly valuable, though cheap for common viruses like TMV. However, depending on the antibody, it may so specific as to record a negative result at the variant level. The test strips and other antibody based methods have to therefore be chosen carefully.

Because of the labor and thought and equipment, the starting point must ultimately be, Is there economic damage to the plant being produced?

I grew the White for years, Tahoe OG too. Though they exhibited some similarity in symptoms to what others have shown in this thread, it did not appear to inhibit growth and production.

That doesn't mean that they don't have a virus. It could indicate that they don't have a virus of economic concern.

What I would love to do personally is to deep sequence one of these suspect plants. That would tell us all even if it has a virus not previously characterized. However, the cost is minimally 800-1000$ USD, finding a lab would be hard, and that's all not to mention the data analysis.

Short of that, I would prefer to run different PCR assays on plant samples. I can run a half a dozen or so, and check for certain virus types. I will do so as soon as possible, but it may take me a few months as I don't currently have access to an operational PCR.

Even if I do though, we won't be able to rule out the possibility of another virus. I simply don't have the ultracentrifuges and other equipment necessary to identify an uncharacterized and unidentified virus.

I appreciate the thread, and I ask that any future Agdia test user please include a picture of your results! I will try to update here when possible.

PS: to the most recent two posters, see this paper: http://www.tandfonline.com/doi/abs/10.1080/03235408.2012.749696

 

[expealadocious]

What an epic thread...
I wonder about the lines of broad mites and this tmv being blurred...

Wild shit , all of it...

 

[birdman_]

Remember a While Ago Seeing the Mosaic Virus - tmv? Tyedye ?? on Mack10's Chem D. So Plain to See " No Extra Zoom Needed ." Just saying..

 

[P. Dondrub]

Please let me reiterate: from a pathologist's perspective, the phrase "a mosaic virus" has little meaning. Preferred is an unidentified cause producing mosaic symptoms. Even if we knew it were a virus, it is remarkable that Cauliflower mosaic virus, Tobacco mosaic virus, and Cucumber mosaic virus have more differences than similarities. Nonetheless, they may have similar symptoms, and oftentimes with viruses, similar management strategies.

Beyond that, let me throw a little heat to the fire. This computer doesn't work well to post the following link, but I think there are several critical implications for us all from studies such as these:


"Complete nucleotide sequence of a cryptic virus from hemp"

http://www.ncbi.nlm.nih.gov/pubmed/22075921

 

[ashna]

Even if I do though, we won't be able to rule out the possibility of another virus
[...]
Please let me reiterate: from a pathologist's perspective, the phrase "a mosaic virus" has little meaning. Preferred is an unidentified cause producing mosaic symptoms. Even if we knew it were a virus, it is remarkable that Cauliflower mosaic virus, Tobacco mosaic virus, and Cucumber mosaic virus have more differences than similarities. Nonetheless, they may have similar symptoms, and oftentimes with viruses, similar management strategies.

Awesome info, thanks.

 

[Bongstar420]

TMV in Petunia

Yeah it does seem like its just a matter of time before mosaic viruses are in ALL cannabis plants and it is getting to the point of being a very serious problem. The reason is so many people grow nowadays that it's spreading rapidly. Most these people are uneducated and/or dont care.



Of course, stress weakens the immune system and gives pathogens and other problems a window of opportunity. It's the same as viruses in humans.




Dude, get some perlite in that medium. Thats no virus. Lack of oxygen in the root zone fasho.

 

[Bongstar420]

Broad mite are not known vectors of virus.

There are some eriophyid mite that do vector though. Rose Rosette Disease is the big one.

http://www.missouribotanicalgarden....d-problems/diseases/viruses/rose-rosette.aspx

Finally, I have broad mite...saw nothing like that from mite.

I did see something nearly identical due to outgassing plastic. Vented the room, plants recovered.

Unless you have a TMV test, you confirmed nothing.

Thanks both for reply.

First , i know broad mites but i want to focus in TMV first altough we know broad mite and thrips are vectors of TMV , so is a problem too and a big one but we can control them with a clean room.

Broad mites come with cuts , my error. But the thing is when arrives TMV and the main thing is not to save these genetics , but what to do in the future.

And after read and see another pics , i can confirm this is TMV in whole garden , not sure which version of five that can attack cannabis plants.

Tomorrow i will upload another pics , specifically after virus come to all plants in the room.

I will show flowering period where TMV is present too so we can see together how evolved in veg and flower room.

For now , while i read and think , i will try to flower all veg room and try to start again new seeds.

 

[_tessarecting]

What was offgassing plastic in your room?

 

[fatalxerror]

Considering the OP put pics of a broad mite infested plant saying it was TMV really has me guessing whether he can identify TMV in cannabis correctly.

 

[Hash Zeppelin]

I had this happen to some purple kush plants once. I killed them all after trying very hard to cure them. I thought it was broad mites. My friend that was growing with me was pissed, but I knew I did the right thing. That shit is nasty.

I'm not a conspiracy theorist, but I'm 99 percent sure that powdery mildew and broad mites are released in to areas with large amounts of growers by the government to fuck up our crops. I talk to growers from the 70s and 80s and they never saw any pm or broad mites until the 2000's. Maybe it's because they were growing resistant land races though, so I could be wrong.

 

[mojave green]

The nasty habit of passing clones around caused the increase, plain and simple.

 

[Hash Zeppelin]

The nasty habit of passing clones around caused the increase, plain and simple.

You're probably right, but clones are required for indoor growing, therefore it's still the governments fault. There would be almost no need to grow indoors if it didn't need to be hidden. You know unless you're in an area far north, that is to cold and has short days. My out door is better than my indoor this year. I have fallen in love with growing out doors from seed. The plants are just so much stronger, and bigger. Plus you get a new type of bud every time you pop a seed. It's exciting.

 

[Ganja baba]

Im curious...
What do you call a 'mutation' that passes to other individuals that had not previously shown any 'disorders' asexually?
What would you call that?

And im not saying it is tmv or isnt tmv...

Im saying its an mv of some sort fyi...

Im curious...
What do you call a 'mutation' that passes to other individuals that had not previously shown any 'disorders' asexually?
What would you call that?



A perfect example of a mutated or genetics that look exactly like tmv is the coffee shop cut o f chocolope , it has genetic mutations but grows fine and flowers big , it's a dead ringer for tmv.
Also does tmv make plants want to pre flower ?

 

[faroutfarms]

TMV?? Help

TMV?? Help

Hello, my name is Eric and I am a caregiver with the state's medical marijuana program.* Let me start by giving you some pertinent*details.* I grow*with CANNA coco coir in 7gal fabric pots.* I use*all high end hydroponic fertilizers and make sure to supplement in silica.* I*monitor nutrient solutions for ph and ec.* I run sealed rooms with CO2.* Temps are low 80's lights on and low*to mid 70's lights off.* Humidity is around 50% in flower and high*50% to low 60% in veg.* Plants flowered under 11 non-air cooled*1000w HPS*lights and vegged with mainly T5 fluorescent with some HID supplementing.* Reservoirs are cleaned weekly and rooms are tore down and scrubbed every 8-10 weeks.* Foliar feed once per week and maintenance spray for pests every 10-14 days.* Plants are watered to runoff 2 times/day, feed in the morning plain water at night(0.3-0.4EC)
*
**I am currently having a problem in my gardens that is getting out of control.* I have been growing indoors for almost 15yrs and thousands of plants and have never seen an issue like I'm currently having.* The plants will thrive throughout the cloning and vegetative processes with little or no plants displaying issues.* within 14-21 days of switching to 12/12 photoperiod I will notice only the leaves protruding from the forming flower start to show signs of chlorosis starting closest to the flower and moving out towards the tip of the leaf.* As it progresses the flower sites will become almost completely "yellowed out" while the fan leaves remain relatively healthy.* Towards the end of flower it becomes apparent that the flowers are failing to bulk up and oil production is severely reduced.* The fan leaves also start to show signs of heat stress during the final weeks.
*
I have been having this issue for over 5 months now.* I*have tried ruling out over fertilization with testing EC of nutrient solution and runoff.* I keep my PH in a constant range of 5.9-6.1 so have ruled out any lockouts due to that.* In the past, I have been able to solve ALL issues I have encountered by either flushing to remove excess salts, paying closer attention to ph, or by foliar feeding for macro/micro deficiencies.* However, with my current situation the problem is only getting worse.
*
I have contacted several testing facilities across the state attempting to find one to test for pathogens.* After speaking with several microbiologists they have led me to the conclusion that I am dealing with an infection by some strain of the Tobamovirus(Sunn Hemp*Mosaic*Virus best guess)*of some sort for 2 reasons. First, not all plants across my 2 flower rooms are affected equally.. Some plants are mostly healthy and some are mostly affected.* There may be a plant that all but 1 branch is severely affected and the branch that isn't seems completely unaffected and is thriving.* On the other hand there may be a perfectly healthy plant with 1 affected branch.* The affectation seems to be indiscriminate across strains and location in flowering room.* Secondly, I have recently put some of my plants in a room with plants from another caregiver that has never had this issue before and within 21 days of flower his plants were also exhibiting signs.* This was done at a completely different location.
*
I have ordered a TMV immunostrip test kit(ELISA) from AGDIA to try and test on my own, but would be more confident in a neg/pos result from a lab rather than an at home test.* As such, I have talked with Alliance Analytical Labs in Coopersville and will be having them verify my at home test with a more controlled laboratory test.
*
I guess my question is has anyone on here ever dealt with some sort of a pathogen in their grow?* If so how did you treat it?* I understand the virus will always exist on some level in my grow, but can it be controlled to the point that I rarely see signs of it?* I was told when I first encountered PM that the only way to never see it was to get rid of everything and start from scratch and found out that wasn't necessary and I have been controlling it for years without seeing any signs.* I'm hoping the same is possible with a viral infection?!

 

[G.O. Joe]

There are many mosaic viruses so testing for TMV may not be very helpful. One thing they probably have in common is a mosaic on the leaves at some point, in my experience nearest to the light, also clones will have it. I treat seeds tools and surfaces with TSP, bleach, then instant dry milk whether it helps or not. Viruses only enter damaged plant parts I think, so I don't even like taking clones much less defoliating, and if that's silly paranoid stupid then so be it.