A post on the subject from Vic High
RRS Improved Breeding for Closet Hacks
Here is a simple model that can help explain how someone with as little as a 400watt garden and a flo clone bench could pull off an improved breeding project utilizing a derivitive of the RRS concept. This is not a strict blueprint, but just a general model that can be scaled up or down depending on the realities of the seed maker. In this case, I will assume that the clone bench is limited to 30 clones and the flower room limited to 20 flowering plants. Purists will find flaws in the model and and their insight is welcome as it can only add depth. However, I request that any that are not interested in the concept of improved breeding techniques to simple limit their participation to read only status. By all means questions about clarification are welcome, but ignorant attempts to discredit are not. As the starter of this thread, I ask the breeder mod to respect this wish and delete any extra "noise". If this isn't possible because of site policy, I will just remove this post and take it to a more selfish location FPRIVATE "TYPE=PICT;ALT="
Hope this enlightens some to the possibilities.
*********************************************
Starting with two unrelated inbred lines, A and B.
1) Grow out the A line and select the top 5 males (Am1-5) and top 5 females (Af1-5) to use later:
a) plant a little over twice the seeds your flower room can handle
b) veg seedlings
c) sex the seedlings
d) select females for flower room and repot into final pots
e) take 4 clones of each of these females
f) flower males and females, may be cramped with the males to start, don't worry
g) just before the males explode, remove, grade, select 5 best.
h) prune these 5 males to remove all flowers and return to veg area
i)harvest females, grade, and identify the top 5
j) keep the best two clones that represents each of the 5 selected females, discard the rest
What you finish with is 15 clones and some sinse to enjoy personally or sell if that is your game. Whatever the route, this step has not cost the grower anything but a bit of space in the flo bench.
2) Grow out the B line and select the top 5 males (Bm) and top 5 females (Bf) to use later: Follow the procedure used in step 1 above.
The net result should be another sinse crop and the flow bench contain 30 clones now.
5 Am clones labelled Am1 - Am5
2 sets of 5-Af clones labelled Af1-Af5
5 Bm clones labelled Bm1 - Bm5
2 sets of 5-Bf clones labelled Bf1-Bf5
3) Making the A and the BA seedlines Take the 5 Am and 1 copy of each of the Bf and Af clones and flower them out (15 total) allowing random pollination. You should end up with 10 seedlots divided into two groups of 5, the A group labelled A1-A5 and the BA group labelled BA1-BA5
4) Making the B and the AB seedlines Take the 5 Bm and 1 copy of each of the Bf and Af clones and flower them out (15 total) allowing random pollination. You should end up with 10 seedlots divided into two groups of 5, the B group labelled B1-B5 and the AB group labelled AB1-AB5.
Be sure to make sure that the end numbers correspond to the same initial female clones. For instance, be sure that AB1 and A1 seedlines are from the same A1 mother. And that the BA1 and B1 seedlines came from the same B1 mother.
The clone bench should be empty now and the breeder should have 5 packs of A seedline, 5 packs of the B seedline, 5 packs of AB seedline and 5 packs of the BA seedline making 20 packs of seed.
5) Grow out and rate the 5 packs of AB seedline. One could do them all at ounce, or divide it up over 6 sinse crops. Nothing wrong with a few sinse crops is there? For this model I will divide the task into the 6 sinse crops.
5a) Grow out as many of the AB1 seeds as your flower room can handle. This usually means planting a little over more than double the number of plants your room can handle and then just selecting the best females for flowering. Make backup clones of each female to be flowered and store them on the flo bench. Now take the number of plants your flowering room can hold (say 20 in this model) and divide this number by 5 and this is the number of keeper plants you want to identify from this crop (say 4). Use a standard score sheet system to score this crop and make notes for future reference if necessary. Identify and select the clones representing these 4 plants and toss the rest. Be sure to label them AB1.
5b-5e) repeat the 5a process for each of the seedlots labelled AB2 to AB5. You have now enjoyed 7 sinse crops and should end up with 20 clones on your clone bench if your flower room can hold 20 plants. There should be 4 of each group, be sure each group is properly labelled from AB1 - AB5.
5f) This is your eighth sinse crop and is where you grow the best of the best of the AB seedline. Randomly arrange these plants in your grow room and then flower them out. Score each plant and use the totals of each group of 4 to score each of the 5 groups. Rank the AB seedlines from best to worst. You can help your decision by referring to the data you also collected in the previous 5 grows. The point is to determine which of the 5 seedlots provided the best OVERALL results, not the best individuals
Your clone bench should be empty again.
6a-6f) repeat the entire process from 5) above, but using the BA1 - BA5 seedlines instead.
7) with reference to steps 5a-5f, use the data data collected to determine your two top A seedlines, lets say that A5 ranked tops and A3 ranked second. This means that you can discard lots A1, A2 and lot A4
7a) Grow out as many plants of the A5 seedbatch as you can and select your top 3 males and top 3 females. Maintain clones of these 6 plants on your clone bench, cleartly labelled as Af1-Af3 and Am1-Am3.
7b) Grow out as many plants of the A3 seedline and select the best 2 males and best 2 females, clearly labelling them Af4-Af5 and Am4-Am5.
Now be sure to have two copies of each Af clone for a total of 15 clones on the clone bench. Sound familiar?
8) Now repeat the processes in step 7 but with using the data collected in step6 to choose the top two B seedlines.
Now you should have 30 clones on your clone bench representing:
5 Am clones labelled Am1 - Am5
2 sets of 5-Af clones labelled Af1-Af5
5 Bm clones labelled Bm1 - Bm5
2 sets of 5-Bf clones labelled Bf1-Bf5
Repeat the process in steps 3) and 4) so that you once again have seedlines labelled at A, B, AB, and BA.
You have finished the first cycle of the breeding program and ready to start a second. It hasn't been quick but you have enjoyed alot of sinse along the way and the combined AB and BA seedlines represent a superior F1 seed product. Each step of RRS will just keep making it better.
Now it's time to post, proof, and go back and edit the mistakes, haha.
figure1.jpg.0bfdea55422fc2240b43fb0bb750877f.jpg
figure2.jpg.ec9514833b521cd13567ba2a666d5b69.jpg
RRS Improved Breeding for Closet Hacks
Here is a simple model that can help explain how someone with as little as a 400watt garden and a flo clone bench could pull off an improved breeding project utilizing a derivitive of the RRS concept. This is not a strict blueprint, but just a general model that can be scaled up or down depending on the realities of the seed maker. In this case, I will assume that the clone bench is limited to 30 clones and the flower room limited to 20 flowering plants. Purists will find flaws in the model and and their insight is welcome as it can only add depth. However, I request that any that are not interested in the concept of improved breeding techniques to simple limit their participation to read only status. By all means questions about clarification are welcome, but ignorant attempts to discredit are not. As the starter of this thread, I ask the breeder mod to respect this wish and delete any extra "noise". If this isn't possible because of site policy, I will just remove this post and take it to a more selfish location FPRIVATE "TYPE=PICT;ALT="
Hope this enlightens some to the possibilities.
*********************************************
Starting with two unrelated inbred lines, A and B.
1) Grow out the A line and select the top 5 males (Am1-5) and top 5 females (Af1-5) to use later:
a) plant a little over twice the seeds your flower room can handle
b) veg seedlings
c) sex the seedlings
d) select females for flower room and repot into final pots
e) take 4 clones of each of these females
f) flower males and females, may be cramped with the males to start, don't worry
g) just before the males explode, remove, grade, select 5 best.
h) prune these 5 males to remove all flowers and return to veg area
i)harvest females, grade, and identify the top 5
j) keep the best two clones that represents each of the 5 selected females, discard the rest
What you finish with is 15 clones and some sinse to enjoy personally or sell if that is your game. Whatever the route, this step has not cost the grower anything but a bit of space in the flo bench.
2) Grow out the B line and select the top 5 males (Bm) and top 5 females (Bf) to use later: Follow the procedure used in step 1 above.
The net result should be another sinse crop and the flow bench contain 30 clones now.
5 Am clones labelled Am1 - Am5
2 sets of 5-Af clones labelled Af1-Af5
5 Bm clones labelled Bm1 - Bm5
2 sets of 5-Bf clones labelled Bf1-Bf5
3) Making the A and the BA seedlines Take the 5 Am and 1 copy of each of the Bf and Af clones and flower them out (15 total) allowing random pollination. You should end up with 10 seedlots divided into two groups of 5, the A group labelled A1-A5 and the BA group labelled BA1-BA5
4) Making the B and the AB seedlines Take the 5 Bm and 1 copy of each of the Bf and Af clones and flower them out (15 total) allowing random pollination. You should end up with 10 seedlots divided into two groups of 5, the B group labelled B1-B5 and the AB group labelled AB1-AB5.
Be sure to make sure that the end numbers correspond to the same initial female clones. For instance, be sure that AB1 and A1 seedlines are from the same A1 mother. And that the BA1 and B1 seedlines came from the same B1 mother.
The clone bench should be empty now and the breeder should have 5 packs of A seedline, 5 packs of the B seedline, 5 packs of AB seedline and 5 packs of the BA seedline making 20 packs of seed.
5) Grow out and rate the 5 packs of AB seedline. One could do them all at ounce, or divide it up over 6 sinse crops. Nothing wrong with a few sinse crops is there? For this model I will divide the task into the 6 sinse crops.
5a) Grow out as many of the AB1 seeds as your flower room can handle. This usually means planting a little over more than double the number of plants your room can handle and then just selecting the best females for flowering. Make backup clones of each female to be flowered and store them on the flo bench. Now take the number of plants your flowering room can hold (say 20 in this model) and divide this number by 5 and this is the number of keeper plants you want to identify from this crop (say 4). Use a standard score sheet system to score this crop and make notes for future reference if necessary. Identify and select the clones representing these 4 plants and toss the rest. Be sure to label them AB1.
5b-5e) repeat the 5a process for each of the seedlots labelled AB2 to AB5. You have now enjoyed 7 sinse crops and should end up with 20 clones on your clone bench if your flower room can hold 20 plants. There should be 4 of each group, be sure each group is properly labelled from AB1 - AB5.
5f) This is your eighth sinse crop and is where you grow the best of the best of the AB seedline. Randomly arrange these plants in your grow room and then flower them out. Score each plant and use the totals of each group of 4 to score each of the 5 groups. Rank the AB seedlines from best to worst. You can help your decision by referring to the data you also collected in the previous 5 grows. The point is to determine which of the 5 seedlots provided the best OVERALL results, not the best individuals
Your clone bench should be empty again.
6a-6f) repeat the entire process from 5) above, but using the BA1 - BA5 seedlines instead.
7) with reference to steps 5a-5f, use the data data collected to determine your two top A seedlines, lets say that A5 ranked tops and A3 ranked second. This means that you can discard lots A1, A2 and lot A4
7a) Grow out as many plants of the A5 seedbatch as you can and select your top 3 males and top 3 females. Maintain clones of these 6 plants on your clone bench, cleartly labelled as Af1-Af3 and Am1-Am3.
7b) Grow out as many plants of the A3 seedline and select the best 2 males and best 2 females, clearly labelling them Af4-Af5 and Am4-Am5.
Now be sure to have two copies of each Af clone for a total of 15 clones on the clone bench. Sound familiar?
8) Now repeat the processes in step 7 but with using the data collected in step6 to choose the top two B seedlines.
Now you should have 30 clones on your clone bench representing:
5 Am clones labelled Am1 - Am5
2 sets of 5-Af clones labelled Af1-Af5
5 Bm clones labelled Bm1 - Bm5
2 sets of 5-Bf clones labelled Bf1-Bf5
Repeat the process in steps 3) and 4) so that you once again have seedlines labelled at A, B, AB, and BA.
You have finished the first cycle of the breeding program and ready to start a second. It hasn't been quick but you have enjoyed alot of sinse along the way and the combined AB and BA seedlines represent a superior F1 seed product. Each step of RRS will just keep making it better.
Now it's time to post, proof, and go back and edit the mistakes, haha.
figure1.jpg.0bfdea55422fc2240b43fb0bb750877f.jpg
figure2.jpg.ec9514833b521cd13567ba2a666d5b69.jpg
I've spent a goodly portion of the past 24 hours thinking about this, googling reciprocal recurrent selection, digging into an old plant breeding genetics textbook, and trying to figure out how to adapt this to my modest grow space. But I reckon its doable, especially if I split some of the stages into more than one grow. 
Thanks again zif for suggesting this, and also MagillaG for the detailed example. Awesome stuff.
