Tutorial DIY STS Mixing/Using Guide for Feminized Pollen/Seeds R.C.Clarke Method as Base

[LostTribe]

Plants that I've reversed do not always drop pollen as we see with a normal male. Most FEM pollen is much more tacky/wet, with very little airborne pollen. It doesn't fall easily. Collection can be time consuming. I pick off any open male clusters and put them into a kief box to dry for 24hrs. It's then screened and collected. Most plants I've reversed had to be done like this. The amount of pollen is far less than what a male would drop. I have had some reversed plants drop normally but it is not something I see often.


I use a STS mix of 1:7. It works most of the time. Much better than 1:9. Some plants are hard to reverse. I've never seen any burn using 1:7. These were sprayed 4x from the start of 12/12. I got 3x more pollen from APPSS than I did NEM. I had to put NEM in with the females to get a good pollination. She won't give up a lot of pollen for later use.. If you use IPM with any oils you must!!!! wash that off before treating with STS!
View attachment 18996979 View attachment 18996978

Much thanks @Hammerhead I'll up to 1:7 immediately and if that dont work I'll give the CS a go with asprin. I havent even been getting much in the way of opening going on. Not watering with silica this time was worried it was hardening them too much or something. The time I did STS on a pair of FDM C99 they both dumped pretty hardcore dusty roads and then some. Thanks again! I did get a tiny bit of Trinity that I forgot aobut off open sacks last time so I tried it on a Trin top and marked the top.

 

[Guy Brush]

I'm throwing in the term cobalt chloride. I think I saw it on overgrow.com in a thread about Humboldt CSI and that they use it for hard to reverse plants. I didn't google it. So lazy today...

 

[LostTribe]

I'm throwing in the term cobalt chloride. I think I saw it on overgrow.com in a thread about Humboldt CSI and that they use it for hard to reverse plants. I didn't google it. So lazy today...

You be the man brother you be the man.

Cobalt Hexachloride 100mg applied to females just the very tips of branches causes tip death and auxillary branching elongation with male flowers. Seems its much more effective at inhibiting ethylene production than silver. I found them trying it on OG and heres the link to the scientific paper.

10mg per liter

Production of Feminized Seeds of High CBD Cannabis sativa L. by Manipulation of Sex Expression and Its Application to Breeding

The use of the cannabis plant as a source of therapeutic compounds is gaining great importance since restrictions on its growth and use are gradually reduced throughout the world. Intensification of medical (drug type) cannabis production stimulated breeding ...

www.ncbi.nlm.nih.gov

 

[Guy Brush]

You be the man brother you be the man.

Cobalt Hexachloride 100mg applied to females just the very tips of branches causes tip death and auxillary branching elongation with male flowers. Seems its much more effective at inhibiting ethylene production than silver. I found them trying it on OG and heres the link to the scientific paper.

10mg per liter

Production of Feminized Seeds of High CBD Cannabis sativa L. by Manipulation of Sex Expression and Its Application to Breeding

The use of the cannabis plant as a source of therapeutic compounds is gaining great importance since restrictions on its growth and use are gradually reduced throughout the world. Intensification of medical (drug type) cannabis production stimulated breeding ...

www.ncbi.nlm.nih.gov

Let us know how it worked if you plan to try the method.

 

[ojd]

I've kept spraying about every 5 days. I'll keep on doing it a bit more and just give it time. I have time. Don't see necessarily new pistils just some on the top. The nanan's are poking out between the sacks too.

Spraying to often gives no pollen sometimes , more than twice is not needed with STS , once when flipped and again a week or 2 later if needed.

I've not had luck with ECSD when tried several times years ago so can't advise specifically for ECSD as it's know to be 1 if not the hardest to reverse

 

[ojd]

I manually pick the flowers off and hand pollinate straight from plant to plant, as stated you get way less viable pollen than a normal Male

 

[LostTribe]

I applied ~30ppm CS yesterday. Will do that for a few days and see. The sacks looks fatter than the other plant I am reversing. They seem to open much slower than a real male and the flowers dont drop down as they are all smashed together. Green bananas show protruding first then seem to sort of age maybe openning slightly in there smashed area without dropping down like a normal male would.

 

[LostTribe]

Spraying to often gives no pollen sometimes , more than twice is not needed with STS , once when flipped and again a week or 2 later if needed.

I've not had luck with ECSD when tried several times years ago so can't advise specifically for ECSD as it's know to be 1 if not the hardest to reverse

Did it used to be keep spraying CS until you get pollen? Thats what I just read but seem to recall that the plants might need you to stop spraying so they can produce pollen, like how you said only spray twice.

The tread post 1 mentioned spraying every 5 days which is what I did this time. Only twice the other 2 tries. Did have a sack open up and fall off which is a good sign. That didnt happen last 2 tries.

 

[Hammerhead]

I've had more failures using 1-2 applications at 1:9. Some with 4 at 1:9 still didn't work well. 1:7 using 4 applications is what works best for me. I make a lot of fem seeds. You guys can play around with this to find what works for you.

 

[ojd]

Did it used to be keep spraying CS until you get pollen? Thats what I just read but seem to recall that the plants might need you to stop spraying so they can produce pollen, like how you said only spray twice.

The tread post 1 mentioned spraying every 5 days which is what I did this time. Only twice the other 2 tries. Did have a sack open up and fall off which is a good sign. That didnt happen last 2 tries.

Yes Colloidal silver you spray every day until pollen drops , but STS spraying doesn't need more than 3 sprays or it can stop it from dropping pollen in my opinion, especially if a strong dose.

Colloidal Silver and STS are different.

If trying to Reverse the ECSD I would stick with STS ( much stronger), I tried many times reversing the ECSD with CS with no luck getting viable pollen.

 

[LostTribe]

I started with 3 sprays STS 1:9 this time, yes its the Sour D. I've just sprayed CS to the same plant 3 days in a row as well ~30ppm. So far looks maybe larger sacks than last time and one of the mature ones sort of openned and fell off without pollen dropping. Only got a little CS left so not sure I'll apply any more this time.

Better lighting this time. Will report back if it works. Fingers crossed.

 

[Miraculous Meds]

Getting some green bananas coming out of the "reverse pollen sacks" with no pollen. Pollen sacks are plentiful and not many hairs. Been using ration from post #1, suggestions? Trinity, Albany, Sour D and one other are all doing the same thing almost zero pollen. Tried a couple times on the sours. Increase the ratio or decrease? I dried a few of the plants before and didnt find any dust inside the sacks.

Suggestions for a higher STS concentration? Whats the highest mix anyones had success with? Measurements if you can help me out, have a and b on hand.

Full strength, no dilution. Just mix them together and use. I never get burning. I make sure they are saturated before spraying. Then spray at night cycle to let dry completely and place in low light (300 to 500 ppfd).

 

[Guy Brush]

Full strength, no dilution. Just mix them together and use. I never get burning. I make sure they are saturated before spraying. Then spray at night cycle to let dry completely and place in low light (300 to 500 ppfd).

Saturated with what?

 

[LostTribe]

Sour D

 

[Hammerhead]

I would not recommend using STS undiluted. Most people will get fried and damaged plants doing this. I've had bad results happen using 5:1 mix.. Its also a waste to use STS like that..

 

[LostTribe]

What is everyone's method for collecting these pollen sacks off the plant when they don't drop pollen? Last one I sort of plucked them one at a time and later used tweezers to agitate and carefully scrape them off into a tiny jar but the later caused the plant to end its lifecycle taking no more water and dying off within a week. I am waiting for this one to give me as many open sacks as possible. Sacks are over 50% open and mostly are turning lighter in color and no longer green so I hope the extra time will provide some result.

The Trinity pollen I collected this way and froze worked to finally s1 it a bit more on an untreated plant painted with brush a la Bob Ross, happy little trees.

I will add that when collecting these sacks it is important not to enclose them in a sealed container until they are dried properly. I did that with another batch off a different cultivar and the sample molded. You want all pollen collection to be dry. I had another batch of fresh screened male pollen that I bagged and forgot to put back out which turned to playdough all stuck together when I went to use it.

 

[LostTribe]

Alot goes a little ways...

 

[Douglas.Curtis]

Number one reason I recommend using multiple clones with multiple schedules and dilution rates, because it's the best way to ensure a high probability of one of them working out. Here's hoping you get more usable pollen the next run.

 

[ojd]

Alot goes a little ways... View attachment 19013664 View attachment 19013665

ECSD is 1 of the worst clones ever to obtain viable pollen , plenty of sacs just empty or with non viable pollen.

Same with the Original Diesel clone
Im reversing an Original Diesel S1 clone now to see if it gives more viable pollen than the ECSD or Original Diesel clones

 

[Hammerhead]

Alot goes a little ways... View attachment 19013664 View attachment 19013665

I use a kief box to sift the pollen from the leaf matter. You should see pollen. If not unlikely there will be any viable pollen. I use folded parchment paper bundles to store pollen.