Chocolate Thai Revival

[Zanddar]

Something like this or more specific?

Veg Boom Plus
1-1-2
The main ingredient in Veg Boom Plus is derived from purified seaweed meal. Plants under stress are unable to produce sufficient cytokinins, the natural plant growth hormones which are necessary for plant growth, nutrient mobilization and distribution, germination, cell division, root development, and flowering. Natural cytokinins such as those found in Veg Boom Plus will help control and regulate: germination, root development, nutrition uptake, plant tissue composition, flowering, seed and fruit set.

 

[Only Ornamental]

Though I'm a fan of natural things, for your purpose I wouldn't use a seaweed extract because they claim whatever they want. Like any plant extract, it does contain other plant hormones as well (under others contra-productive ones too) and as you know, it's the ratios and the absolute amounts that count. Even if we were to know even one of the two in said product, their composition due uncontrolled degradation will change over time and what you actually buy isn't in any way what it was when (if) they tested it.
Sure, you can try it. Seaweed extracts have a lot of benefits but seldom those mentioned in the advertisements.

 

[idiit]

^ that is not an exposed embryonic "tap" showing in the above third pic. it is actually an adjacent part of the embryonic cotyledons.

unfurling germination attempt. black embryonic sheath very carefully removed and root tip placed downward into sterilized fresh potting mix for seedlings (fafard #2), (1/1/1 ferts added by mfg.). the root tip actually curls back up towards the white embryonic cotyledons so actually very little potting mix covering the root tip. under very cool cfls. two actual seedlings developed enough to undergo this germination attempt. the root tails do not manifest in these seed embryos while in paper towels. these old seeds don't have the mojo left in them to develop normal root tails. this is why they are helped manually. when an older seed pops and just "unfurls" you don't get a root tail. they can and have germinated into healthy seedlings for me using this technique which is why i'm posting pics of the stages. will continue to update with photo documentation.

zanddar's ct's haven't even shown any seeds to unfurl white, healthy "un-furled stunted taps" yet. in his example unless we get a few to at least unfurl white healthy "taps" he will need to investigate sterile "petri dish" style germination attempts imo. samtheskunkman has done a lot of older seed germination runs and might be a valuable source of info.

 

[neongreen]

Though I'm a fan of natural things, for your purpose I wouldn't use a seaweed extract because they claim whatever they want. Like any plant extract, it does contain other plant hormones as well (under others contra-productive ones too) and as you know, it's the ratios and the absolute amounts that count. Even if we were to know even one of the two in said product, their composition due uncontrolled degradation will change over time and what you actually buy isn't in any way what it was when (if) they tested it.
Sure, you can try it. Seaweed extracts have a lot of benefits but seldom those mentioned in the advertisements.

I always soak some seaweed meal in water for a few hours/overnight, and add some of the resulting seaweed extract to my "seed soak". It seems to work a treat, and I get close to 100% germ on my decade old seeds.

 

[idiit]

^ as stated above, 2 seedlings developed white stunted "taps" enough to undergo "un-furl technique". the second embryo was placed into the potting mix after the above pic. in post #145. the critical item here imo is to gently tamp down the potting mix around the embryonic meristem and cotyledons so that they don't 'touch rot' from contact with the moist potting mix substrate.. because the stunted "tap" curls back up towards the surface it is a touchy stage keeping the wet potting mix off the green above ground growth and at the same time keeping the 'tap' under ground level so it can develop a root tail. greening up nicely in the past 24 hours.

 

[wegobigupnorth]

I smoked some amazing chocolate thai in high school. Only came around once and I bought a ton of it and I actually think of it often. It is one strain that REALLY stood out. It for sure was imported.... You could tell right away why the shit is called chocolate thai...It was brown but a chocolate brown. Came bricked with seeds, was very dense and sticky and smoke was soo dense and strong tasted like coco...Yes, like fucking chocolate and had that amazing coco chocolate smell. Just lovely.

 

[wegobigupnorth]

.

 

[idiit]

unfurl technique progress 5 days after being placed in potting mix. been re-potted one time due to original potting mix looking a little fungus prone. very tricky stage. the seedlings have almost no vigor which is the very reason imo they can't establish root tails.which is the very reason they are being helped manually. mold/fungus issues can be deadly and this is not in a sterilized environment. i have had some plants make it using this technique in the past which is why i'm photo-documenting this run as it happens, so ppl can see. none of the other seeds made it. they just unfurled a little or showed some white without any establishing tap roots. then they died. at least these two have a chance. that's all they got; a chance.

 

[Zanddar]

What are you trying to germ there idiit?
You can only work with what you got.


Just waiting on my new cc and have to order some things.
GA3 has arrived and want some other stuff to work in a sterile environment with.
Will do some different tests on what I have not tried yet.

Nothing has worked so far.

 

[idiit]

^ this strain i've been posting the unfurl technique on is an old "elvis" seedstock.

what needs to happen for the very old seedstock is a sterile, high end scientific method imo. i had posted that ppl were not photo-documenting their positive results with various suggestions so i am really just documenting the "unfurl technique" so ppl can see for themselves as it proceeds. don't know if either of these plants will make it or not. some have in the past.

i've spent some time this morning thinking about how to get your ct beans to successfully sprout. we need to also investigate a method that will revitalize the seed embryos as it's the lost vitality that's keeping them from doing anything other than a few/ocassional feeble "unfurls".

i'm thinking that sea-crop is high in monoatomic ormus and rich in chelated micro-nutrients. i use a little magnet gadget that nevil actually recommended first that activates ormus into a more dynamic state. ormus can penetrate the seed cover and possibly revitalize the embryo. i know it's way woo woo but i know of no other element other than monoatomic ormus that is purported to have this revitalizing quality.

run an ormus solution (sea-crop diluted per instructions) through the magnetic gadget along with the seeds. perhaps even try germinating the seeds after being run thru the magnets in a sea-crop solution in paper towels using a very diluted distilled water/sea-crop solution to wet the paper towels.

^ the key here is how do we revitalize the seed embryos so that they have the mojo to develop normal root tails and get along with the entire germination gig.

so two separate paths; one sterile petri dish type germination tech., one woo woo revitalize the actual embryo inside the seed.

 

[idiit]

one of the two 'unfurls' looks like it'll make it.

notice the extreme lack of vigor, low, ebbing vitality, life force weak.

when ppl talk about germinating old seed stock the vitality issue needs to b addressed imo.

what means do we have to rejuvenate the embryo's vitality?

 

[neongreen]

what means do we have to rejuvenate the embryo's vitality?

Apart from moly-coddling/praying, I think the only option would be tissue-culture.

 

[idiit]

I think the only option would be tissue-culture.

^ now we are on to something possibly. can we get tissue cultures from cracked embryos?

zanddar, you up to the tc challenge?

 

[Only Ornamental]

...can we get tissue cultures from cracked embryos?...

Yes, it's possible, feasible, and a good idea if you have the equipment for it. The good thing with using whole seedlings is that you don't necessarily need hormones.

 

[TangieFanatic]

Hi,
Those new embryonic leaves should be enough plant material for tissue culture. I suggest contacting a few of the specialists in tissue culture to see what they have to say. Here is one you might contact https://www.agrimabotanicals.com/cart/agrima-blog/exploring-marijuana-and-tissue-culture.html

I would highly suggest keeping all the seeds that are dead or don't germ and saving them. Some people seem to think that dead seeds will never work with tissue culture, but I think in the near future there will be a way.

I am subbed and excited for you!!! Good luck on your chocolate thai journey!!!

^ now we are on to something possibly. can we get tissue cultures from cracked embryos?

zanddar, you up to the tc challenge?

 

[Zanddar]

Only have the GA3, but have not tried it yet. Found a site with some other hormones and stuff, but have not ordered anything yet.
Willing to try whatever you think will work. I know they need more than what I have here.
Soaking the seeds briefly in a cleaning agent has stopped the rotting. Have some sterile containers now too.

 

[TangieFanatic]

Oops, I confused another users picture of a sprout as yours.

I have faith in you!!! Remember to save any dead seeds and of course save all plant matter, stems, leaves, and flowers. Since you may not have time to contact some tissue culture experts, I will contact some of them tomorrow and find out more information that could help your situation. This is going to be so much fun.

Only have the GA3, but have not tried it yet. Found a site with some other hormones and stuff, but have not ordered anything yet.
Willing to try whatever you think will work. I know they need more than what I have here.
Soaking the seeds briefly in a cleaning agent has stopped the rotting. Have some sterile containers now too.

 

[idiit]

This is going to be so much fun

^ yes, it is fun. better than bass fishing imo .

the good news is zanddar has talent and many of us here are not only along for the ride but willing to help him source the proper technique for him to successfully sprout the beans instead of wanting him to outsource. could be a very educational ride for all of us.

 

[TangieFanatic]

Yeah, fishing can get a bit boring, unless you have plenty of dank and you're fishing in a fjord up in B.C or somewhere nice.

I contacted 3 or 4 of those tissue culture specialists on the net. Not a single one ever replied to me.

After looking at how much the tissue culture kits cost on Amazon, I think they are out of most people's price range. You can buy all the materials and stuff separately for much cheaper but you have to really understand what you're doing. After watching tutorials on the process, it seems daunting to say the least.

I think it's very likely the process can be done with a live cracked seed with a tap root. I think if you dissected the cracked seed you could use the microscopic embryonic leaves for culture. I wish I had a kit and some beans to play with.....

^ yes, it is fun. better than bass fishing imo .

the good news is zanddar has talent and many of us here are not only along for the ride but willing to help him source the proper technique for him to successfully sprout the beans instead of wanting him to outsource. could be a very educational ride for all of us.

 

[GoodTimeHealing]

Love seeing new strains emerge---best of luck, how long u think she will go 10-11 weeks