Cannabis grafting

[St. Phatty]

Seems like it might work better if you have 2 rooted plants, then trim the outside of the stem on one side and join them there. Once the plant that is borrowing the root system is joined to the new plant, it can be cut off from its root system.

I would only expect about 20% yield.

It's just a SWA Guess, never tried it.

 

[Darpa]

Hi folk,
just trying Something different. On the master crooping multi graft plant were a FaceOffxWTC male scion that I used to polinate the BikerKush x (Original Glue4(GSC x TrainWreck)…



So this time I'll try to graft 3 seedling to create a unique plant for genetic selection.








Darpa

 

[bsgospel]

Cool, what's going into this new 3-strain mix? Faceoff x biker x ?

 

[zif]

So this time I'll try to graft 3 seedling to create a unique plant for genetic selection.

Are you going to let forced proximity graft them (as with the braided ficus sometimes sold as houseplants), or are you actively joining them? Can’t tell if you’ve exposed any cambium where they meet.

Easy seedling grafting feels like the next frontier for lowering plant counts.

 

[Darpa]

Cool, what's going into this new 3-strain mix? Faceoff x biker x ?

Hi Bsgospel!

This is a cross of my BKGW x The Sweeties from Archive.

Detail:

BKGW is a cross I made of Karma Biker Kush x Cannaventure Gorilla Wreck. I made a selection over hundred of seed grow over the last few years.

Cannaventure Gorilla Wreck is a cross of Original Glue4 x Cookie Wreck

Cookies Wreck is a cross of Girl Scout Cookies x Arcata TrainWreck

Archive Sweeties is a cross of FaceOff Og x White tahoe Cookies

So this grow is:

((Biker Kush x ((Original Glue4 (GSC x TrainWreck))) x (FaceOff x WTC))

My BKGW is a monster and perform real good in my cold climate, but I wanted to add a twist from the Sweeties

Darpa

 

[Darpa]

Are you going to let forced proximity graft them (as with the braided ficus sometimes sold as houseplants), or are you actively joining them? Can’t tell if you’ve exposed any cambium where they meet.

Easy seedling grafting feels like the next frontier for lowering plant counts.

Hi Zif,

At this stage, I am going to let the seedling grow a little bit taller and perform a second braiding as the shoots are soft and malleable. But when the trunks are going to be about 2 to 3 mm thick, I'll expose the cambium and to a temporary wrapping for a solid graft. The purpose of this is to make a unique plant completly fused together.

Darpa

 

[bsgospel]

How will you know which rootstock is present? Or is it important?

 

[Darpa]

How will you know which rootstock is present? Or is it important?

The rootstock was a female BKGW and the male scion was The Swetties. But It's Nothing different than breeding two different speciment.

The offspring are a cross of BKGW and The Swetties

 

[Hookahhead]

Thanks for sharing Darpa, always great to see your experiments and results. I still need to get my feet wet with cannabis grafting, I just haven’t found a proper application for my setup just yet. Have you tried to graft a flowering scion (week 3-6) on a vegetative stock, effectively monster cropping the scion?

 

[Darpa]

Thanks for sharing Darpa, always great to see your experiments and results. I still need to get my feet wet with cannabis grafting, I just haven’t found a proper application for my setup just yet. Have you tried to graft a flowering scion (week 3-6) on a vegetative stock, effectively monster cropping the scion?

Hi Hookahhead! Thank for your interest! Unfortunately I didn't had the chance to experiment grafting with autoflower scion or auto in flowering stage yet….

I'll let you know when I'll experiment grafting auto scion!

Cheer


Darpa

 

[Darpa]

Hi Folk,
here is a quick update.

I didn't to expose the cambium yet…. I will be waiting at least 2 weeks…






Darpa

 

[Darpa]

Hi folk,
Here is a quick update of the 3 seedlings graft. The smell of that cross is amazing. I can't wait to see the final result! Let's call it the Sweet BKGW.









Cheer!


Darpa

 

[F2F]

@Darpa_which method is best?

@Darpa_which method is best?

Hi Darpa,

So I read through the entire thread again simply amazing work and contribution to the community by you. Sincere thanks.

Also have to shout out to to SamS. Obviously he's light years ahead of many of us on this and other advanced techniques.

In summary, what is the best method you used to graft? Is it the scion notched/wedged into a matching cut on a branch of the rootstock? Other? Also, have you learned any particular treatment or prep of the donor or acceptor plant helps with success?

Thanks in advance!
F2F

 

[Darpa]

Hi Darpa,

So I read through the entire thread again simply amazing work and contribution to the community by you. Sincere thanks.

Also have to shout out to to SamS. Obviously he's light years ahead of many of us on this and other advanced techniques.

In summary, what is the best method you used to graft? Is it the scion notched/wedged into a matching cut on a branch of the rootstock? Other? Also, have you learned any particular treatment or prep of the donor or acceptor plant helps with success?

Thanks in advance!
F2F

Hi F2F! Thanks for the kind words! I realy appreciate that you enjoy my post.

To answer you question, there is not a best method to use graft, each method have it's advantange and inconvenient... It all depend on your goal. For someone looking to create a grafted plant in order to preserve a genetic library, all methods work just fine. For someone who is looking to grow several genetic with a limited plant count number, the best by far is the technique use in post 179 (several graf on a naked rootstock)

Personnaly I prefer to work with micro scion and smaler rootstock plant since the bagging technique use in post 254 is time consuming and you don't have a perfect control of the graft environment…

I prefer to work with auxilary buds micro scion as in post 113 (chip graft) and the method use in post 161 show great advantage since it allow you to make 2 news branch in one single graft.

I didn't start this thread, but If I did, I wouldn't wrote about grafting in the Advance Growing section, since grafting is a fairly easy technique. I cannot wait to post about Tissue Culture Experiment .

As I said, it all depend of your goal! If you have a grafting planning, do not hesitate to post about it and I'll let you know which technique I would use.... It's always nice to share knolwedge.

Unfortunately, most of my experiments do not follow proper scientific method as I'm use to do (control speciment, Quality control...) but I guess that's better than nothing..

Here is an example of a grafting I would do for this FaceOff Og plant. FO OG seem to respond pretty bad to topping and express extra long internode compare to the strains I use to work with.

Here is a comparaison with a Karma Biker Kush (on the right).



So for this FO OG plant that got topped several time, I would take auxilary buds from the upper top (instead of just pinching and tipping) and graft them within the long internode in order to make a a bushier plant. (see post 161).






Yellow line: long internode
Red: Where I woud make a longitudinal cut (with a scalpel blade, through the branch and then cut down… you could use anything to keep the cut open as you work (paper clip))
Pink: Where the auxilary bud would make new branch

As I said, I all depend of your goal!

Concerning your question : have you learned any particular treatment or prep of the donor or acceptor plant helps with success?: Grafting is prety simple and the rootstock and scion do not need a special treatment. I only use basic aseptical technique to make the cut, and use sterile blades but it's not necessary... you could probably use an X-acto blade without wearing glove and everything would be just fine....

Cheer and thanks for you interest!!!

Darpa

 

[Darpa]

Hi folk!
Here is an update on the seedling graft…

Before I post picture, I want to apologize for the quality of the pictures. I realy need to upgrade my camera

So here's the plant (3 seedling)



Close up on the threding that was performed.



I used a scalpel blade to expose the cambium at the base of each plant.



Since I didn't have much space to work, I decided to perform a truck graft combine with a rooting propagation in order to make a single fused plant.

 

[Darpa]

I use a jiffy to wrap the treated area in order to promote roots grow and trunk fusion.



Here is the final result:



I'll unwrap the base on the plant in about 1 Week… I'll post the result…

Cheer

Darpa

 

[Darpa]

Hi Folk, I Don't want to be off topic, since this thread is about grafting… If the moderator or the folk here want me to create a thread Darpa Experiment… Let me know…

But this post could probably answer a question from F2F about scion preparation.

Here's a contaminated scion-cut sterilisation protocol that I'm presently testing (my own protocol).

Aphnid contaminated plant got from an elite plant grown outside. (see thread Forums > Marijuana Growing > Indoor Grows - Soil > Cloning, late flower??? to get my tutorial on monster crooping from late flowering cuts used for clonning)



Plant material selection:



Cuts preparation:



Insecticidal soap - 3 washes then a quick wash before bleach wash...

 

[Darpa]

Bleach wash X3-10%



Now your cuts are ready to be used for grafting or for clonning.

Here is an example of what I did... in order to preserve the cultivar bugs free... Roots hormones on sterilyzed plant explants.



The rest is just basic cloning techniques....





Cheer


Darpa

 

[Darpa]

Hi Folk,
This is a pre-test for the upcoming cold storage of cannabis scion for a grafting purpose.

I just wanted to make sure that my desinfection protocol would be sufficient for cold scion preservation. I order to test it, I used heavy contaminated plant material from the Bud to clone experiment on an other thread (https://www.icmag.com/ic/showthread.php?t=249407&page=3)

8 scions were collected. I would have prefer t use auxiliary bud from a bigger steem instead of terminal apical buds with dormant lateral bud, but we will see how it turn out.

(I wanted to test it on 100 scions, with different formulation but I'll have to wait before the real experiment... this is just a preliminary test)

My intention is to test protocol that every body interested in grafting could do in their kitchen (DIY Kitchen style). That been said, I've bid on a cryobiological storage system that would be quite usefull for my TC experiment.





For those of you in Canada looking for afordable lab equipment, I get all my gear from GCSurplus, of Public Services and Procurement Canada (sells a wide selection of Government surplus assets to the general public)

So for this experiment, I've search over few scientific publication available as:

Effect of scion stick storage on growth and success softwood grafts of sapota cv. KAUPATTI.
Author(s) : Tandel, Y. N. ; Patel, C. B.
Author Affiliation : Department of Horticulture, Soil and Water Management Research Unit, Navsari Agricultural University, Navsari (Gujarat), India.
Journal article : Asian Journal of Horticulture 2009 Vol.4 No.1 pp.198-201 ref.8

I don't want it to be repetitive but here is what i'Ve done:

Scion selection (on available material)



Scions preparation:

 

[Darpa]

Scion desinfection: Soap - bleach





Hald of the scion were sealed with parafin (left), as recommended on some scientific publication. I would have prefer to use formulated arginate but I didn't have it on hand at my house (in my lab)...



Treated scion were wraped papertowel (instead of newpaper as refered in SP) and then in plastic bag



I stored them in the fridge (light deprivation).
I'll check on them in 7 days...

Cheer

Dapra