-
ICMag with help from Phlizon, Landrace Warden and The Vault is running a NEW contest for Christmas! You can check it here. Prizes are: full spectrum led light, seeds & forum premium access. Come join in!
CANNABIS DNA PROJECT
- Thread starter Sam_Skunkman
- Start date
Lets stick to the DNA project or I will just remove the off topic posts in my thread.
Forum Facism takes many forms, you violate the TOU and yet call others names, yes why don't you look up the USA Cannabis laws having to do with seeds, it only takes a few minutes to find the truth. What was your truth that I am patenting Cannabis? How about the patent # with my name on it?
Until then I guess I will shun you, not for being inquisitive, but for being just so wrong.
-SamS
Forum Facism takes many forms, you violate the TOU and yet call others names, yes why don't you look up the USA Cannabis laws having to do with seeds, it only takes a few minutes to find the truth. What was your truth that I am patenting Cannabis? How about the patent # with my name on it?
Until then I guess I will shun you, not for being inquisitive, but for being just so wrong.
-SamS
Will this end up with a nice and easy table to show relations of different genotypes or will it be more of a list? I am easier reading something like a chart than 50 + pages of findings, I will still read them but the chart usually helps it sink in a bit more.
It will be both and more, we have software that makes the data point of each sample a different point in a constellation, you can touch any point and see the data from that point, zoom in and see families and relationships, back away and see every data point. As soon as I can I will try and have the info available to be viewed, we have not yet analyzed the landrace populations, we started with local USA hybrids and have done maybe 1000 of these, we have also started on the 250 samples, mostly landraces I have collected up till now, I also have my personal collections to go through and find more to test. We have not yet started with samples that are hundreds or thousands of years old, we have quite a few, but want to be sure that our ability to get the DNA in good shape from antique samples is as good as possible before we try with the very old and very rare samples I have collect during my life. Should be interesting, but it does take time, we will be at this for several years.
-SamS
-SamS
This would be pretty cool. I'm betting you have some old exotic possibly extinct lines in seed form Sam. Colombian wacky weed perhaps?
The D3 lib has great ways to show the data, here are some examples similar to constellation...
http://bl.ocks.org/paulovn/9686202
http://www.brightpointinc.com/interactive/political_influence/index.html?source=d3js
http://mbostock.github.io/d3/talk/20111116/force-collapsible.html
http://bl.ocks.org/paulovn/9686202
http://www.brightpointinc.com/interactive/political_influence/index.html?source=d3js
http://mbostock.github.io/d3/talk/20111116/force-collapsible.html
The purpose is to discover Cannabis family relationships and Cannabis evolution as we build a Cannabis family tree.
While I personally am interested in old lines, the DNA project just wants the info we can gather from old seeds and old leaf samples, the project does not grow or maintain anything other then the database of info from the DNA. That does not equal a seed line, you need a live seed or seeds to keep that maintained, and we only keep the DNA info. We also plan to share that info.
The data base will be useful in the future to see if a given sample is what it is supposed to be, if two samples are the same clone or variety, and things like that.
It is the potential to reveal evolution that I find the most interesting, where did Cannabis originate, what populations were taken to India, Africa, the new world? From where?
I personally am interested in NLD varieties that can be grown nearer the equator and are up, clear, psychedelic, euphoric, cerebral, energetic, speedy, tasty with good vigor and pest & disease resistance, and strong as hell. I want to have more NLD lines that can be grown outdoors, be used to make dry sift hash, and are just great Ganja. To me WLD, Indicas, now dominate the drug gene pool, it would be nice to have more choices?
But the two wants are separate, DNA is pretty easy to collect, NLD varieties that are what I want personally is more work, and they need to be grown by me outdoors to understand their potential as is or as a NLDxNLD hybrid. I will need to GC the mature flowers to understand the Cannabinoids and terpene profiles. Colombian is a good place to start, I love the best of them.
-SamS
I will try and start to make a list of what would be interesting for us, as well anyone can make suggestions here of materials we should looking at and I will add it.
Older samples are better because they are less likely to have foreign genes?
We can use dead seeds, dead leaves, live seeds, live leaves, dead pollen, live pollen, from:
--------------------
All landrace seeds/leaves/pollen, drug, hemp or wild or Ruderalis. WLD or NLD, WLH or NLH,
(Wide Leaf, Narrow Leaf, Drug or Hemp)
Maybe hybrids made with one or more of the above, if we also get the parents used to make the
hybrids, so we can do multi-generational studies.
Sterile plants, you will need to have a leaf.
Almost sterile plants to see why most seeds do not form. check the few seeds/and a leaf.
Intersex plants, various kinds, some with genes for intersex and some with genes that allow
intersex expression with the right conditions.
Monoecious plants, like some hemp varieties.
All female plants XX
All male plants YY
Varigated plants, if possible leaves both variegated and not from same plant?
Anything Auto, even if no THC.
Ruderalis seeds pure Ruderalis and F1 hybrids nice if we also get both parents used in the hybrid
Multi generational samples like F1, F2, F3, F4, including the original parents if possible.
Samples from a female clone and the selfed S1 offspring
Male fasciated plants, or Female
Whorled Phyloxy, Male or Female
Ducksfoot
Polyploids, tetraploid, triploid, diploid, aneuploid
ABC Australian Bastard Cannabis, Mongy, Dizzy, Bindy, Rasta?
Any thing more? Like Pollen? Hemp textiles, Cannabis stalks.
-SamS
Older samples are better because they are less likely to have foreign genes?
We can use dead seeds, dead leaves, live seeds, live leaves, dead pollen, live pollen, from:
--------------------
All landrace seeds/leaves/pollen, drug, hemp or wild or Ruderalis. WLD or NLD, WLH or NLH,
(Wide Leaf, Narrow Leaf, Drug or Hemp)
Maybe hybrids made with one or more of the above, if we also get the parents used to make the
hybrids, so we can do multi-generational studies.
Sterile plants, you will need to have a leaf.
Almost sterile plants to see why most seeds do not form. check the few seeds/and a leaf.
Intersex plants, various kinds, some with genes for intersex and some with genes that allow
intersex expression with the right conditions.
Monoecious plants, like some hemp varieties.
All female plants XX
All male plants YY
Varigated plants, if possible leaves both variegated and not from same plant?
Anything Auto, even if no THC.
Ruderalis seeds pure Ruderalis and F1 hybrids nice if we also get both parents used in the hybrid
Multi generational samples like F1, F2, F3, F4, including the original parents if possible.
Samples from a female clone and the selfed S1 offspring
Male fasciated plants, or Female
Whorled Phyloxy, Male or Female
Ducksfoot
Polyploids, tetraploid, triploid, diploid, aneuploid
ABC Australian Bastard Cannabis, Mongy, Dizzy, Bindy, Rasta?
Any thing more? Like Pollen? Hemp textiles, Cannabis stalks.
-SamS
Last edited:
Sam Leaves will being drying tonight ! will send you a complete collection of everything in the garden Auto's also .
I don't have many landraces growing now but I will fully cooperate with you over the next year or so as I begin to pop the collection .
I have some of the Chinese from Yunan 2005 here also will pm you with the full list of what I have for you now .
Charles
I don't have many landraces growing now but I will fully cooperate with you over the next year or so as I begin to pop the collection .
I have some of the Chinese from Yunan 2005 here also will pm you with the full list of what I have for you now .
Charles
If anyone has DNA samples for us to test please PM me.
Even dead seeds, can have their DNA extracted and sequenced.
Post 67 in this thread is some of what we want, LANDRACES is number one.
Post #1 and #45 in the other thread explain a little more.
https://www.icmag.com/ic/showthread.php?t=296538
Any questions you can PM me.
-Sams
Even dead seeds, can have their DNA extracted and sequenced.
Post 67 in this thread is some of what we want, LANDRACES is number one.
Post #1 and #45 in the other thread explain a little more.
https://www.icmag.com/ic/showthread.php?t=296538
Any questions you can PM me.
-Sams
S
sourpuss
Here we go homies... send in your elite clone only dna samples plzzzzzzzz....
We can now discover whats real and whats not. Whats the true lineage on ecsd??? Og kush??? Blueberry og(original), the truth behind nl 5, chemdawg, romulan.... whos cut is real???
Really gonna open our eyes to the whole industry....
S
sourpuss
I have access to a bag of romulan. Prob a bx from ogstock. Will see if I can send in a sample....
I can use a dead dry leaf. Or dead seeds.
I don't need or want the clones.
So just PM me.
-SamS
I don't need or want the clones.
So just PM me.
-SamS
to Sam. Is there gonna be a website in the future with say a list of landraces and bread strains and there roots on this planet? how long till u start getting some info worth sharing?
The Lebanese community of South America was recently mentioned in a thread here. Maybe youll find some leb genes in some of those strains?
The Lebanese community of South America was recently mentioned in a thread here. Maybe youll find some leb genes in some of those strains?
Yes we will share the data with the contributors and the public. We will post the info on a Phylos or Cannabis Evolution Project www site. I hope real soon we will have data posted.
-SamS
-SamS
Hi Sam,I only just saw this thread as I've been away from IC and the web in general for a while again(usual reasons-I won't bore you). If you are interested I have more dead-collected seed's than I like to be reminded of TBH. I'm not back at my place until next week,but I will gladly sort through my pips then and let you know what's interesting:
I definitely have a bunch of African lines I collected(1 from Transkei I think you might find interesting especially),2 from Brazil,lots of Thai's and other Asian lines old and recent,1 oldie from Mysore and 1 from near Kerala,an Afghan family strain(sent to me) from '81 that's not germinated ever,a couple of old Jamaican lines.......There's other stuff too,I will message you when I have a better Idea and see if they are useful. JBo
