Bigcrawdaddy2.0
Well-known member
Thank you both! I’ll be testing my stuff as soon as I can
-
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Are there any reputable clone vendors in USA??????????
- Thread starter Loc Dog
- Start date
acespicoli
Well-known member
Some cuts gonna be viroid resistant and never show a sign and still be carriers
thru meristem culture they can be cleaned up, long slow process...
S1's of strains that show can be selected clean
time and money ...
thru meristem culture they can be cleaned up, long slow process...
S1's of strains that show can be selected clean
time and money ...
Bigcrawdaddy2.0
Well-known member
I’m in an illegal state. That tissue culture stuff would be nice but not feasible for a guy like me. I heard it’s super expensive too, is that true?Some cuts gonna be viroid resistant and never show a sign and still be carriers
thru meristem culture they can be cleaned up, long slow process...
S1's of strains that show can be selected clean
acespicoli
Well-known member
If you get pro lab work done for sure $$$$
First book I got on it was like 20 years ago
- Publisher : Timber Press; 3rd edition (October 28, 1996)
- Language : English
- Hardcover : 240 pages
- ISBN-10 : 0881923613
- ISBN-13 : 978-0881923612
Murashige and Skoog medium - Wikipedia
Buy agar
save old baby food jars canning jars etc...
make a diy air flow hood wash with dilute bleach work in boxers...
Plants From Test Tubes Holly Scoggins : Free Download, Borrow, and Streaming : Internet Archive
Tissue culture
archive.org
Dont buy these seperate get a premix of it just add distilled water and agar
Ingredients
Mammillaria miegiana on liquid MS mediaMajor salts (macronutrients) per litre
- Ammonium nitrate (NH4NO3) 1650 mg/l
- Calcium chloride (CaCl2 · 2H2O) 440 mg/l
- Magnesium sulfate (MgSO4 · 7H2O) 180.7 mg/l
- Monopotassium phosphate (KH2PO4) 170 mg/l
- Potassium nitrate (KNO3) 1900 mg/l.
Minor salts (micronutrients) per litre
- Boric acid (H3BO3) 6. 2 mg/l
- Cobalt chloride (CoCl2 · 6H2O) 0.025 mg/l
- Ferrous sulfate (FeSO4 · 7H2O) 27.8 mg/l
- Manganese(II) sulfate (MnSO4 · 4H2O) 22.3 mg/l
- Potassium iodide (KI) 0.83 mg/l
- Sodium molybdate (Na2MoO4 · 2H2O) 0.25 mg/l
- Zinc sulfate (ZnSO4·7H2O) 8.6 mg/l
- Ethylenediaminetetraacetic acid ferric sodium (FeNaEDTA) 36.70 mg/L
- Copper sulfate (CuSO4 · 5H2O) 0.025 mg/l
Vitamins and organic compounds per litre
- Myo-Inositol 100 mg/l
- Nicotinic Acid 0.5 mg/l
- Pyridoxine · HCl 0.5 mg/l
- Thiamine · HCl 0.1 mg/l
- Glycine 2 mg/l
- Tryptone 1 g/l (optional)
- Indole Acetic Acid 1-30 mg/l (optional)
- Kinetin 0.04-10 mg/l (optional)
could you imagine getting your clone in a cryo tube used for seeds?
>Best>ibes>>>
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compoundcoaltrain
New member
I don't sell clones this is one flower areas, room picture taken mid way in it , of a ....few. It takes money to hold onto genetics and pump them out , properly. My point was as I stated, i dont have a huge setup i know what my bill is imagine a real Nursery. I'll show you a pic of a light bill if you say pretty please
Attachments
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Deleted User 0283844
Well-known member
I don't sell clones this is one flower areas, room picture taken mid way in it , of a ....few. It takes money to hold onto genetics and pump them out , properly. My point was as I stated, i dont have a huge setup i know what my bill is imagine a real Nursery. I'll show you a pic of a light bill if you say pretty please
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Do u just dm farmer joe on ig for list n pricing?
Bigcrawdaddy2.0
Well-known member
Are you a
?
Can lophophora williamsii be grown faster through tissue culture?If you get pro lab work done for sure $$$$
First book I got on it was like 20 years ago
View attachment 18959559
Every hobby I get into ends up costing me money some how...
- Publisher : Timber Press; 3rd edition (October 28, 1996)
- Language : English
- Hardcover : 240 pages
- ISBN-10 : 0881923613
- ISBN-13 : 978-0881923612
Murashige and Skoog medium - Wikipedia
en.wikipedia.org
Buy agar
save old baby food jars canning jars etc...
make a diy air flow hood wash with dilute bleach work in boxers...
Plants From Test Tubes Holly Scoggins : Free Download, Borrow, and Streaming : Internet Archive
Tissue culturearchive.org
Dont buy these seperate get a premix of it just add distilled water and agar
Ingredients
Mammillaria miegiana on liquid MS media
Major salts (macronutrients) per litre
- Ammonium nitrate (NH4NO3) 1650 mg/l
- Calcium chloride (CaCl2 · 2H2O) 440 mg/l
- Magnesium sulfate (MgSO4 · 7H2O) 180.7 mg/l
- Monopotassium phosphate (KH2PO4) 170 mg/l
- Potassium nitrate (KNO3) 1900 mg/l.
Minor salts (micronutrients) per litre
- Boric acid (H3BO3) 6. 2 mg/l
- Cobalt chloride (CoCl2 · 6H2O) 0.025 mg/l
- Ferrous sulfate (FeSO4 · 7H2O) 27.8 mg/l
- Manganese(II) sulfate (MnSO4 · 4H2O) 22.3 mg/l
- Potassium iodide (KI) 0.83 mg/l
- Sodium molybdate (Na2MoO4 · 2H2O) 0.25 mg/l
- Zinc sulfate (ZnSO4·7H2O) 8.6 mg/l
- Ethylenediaminetetraacetic acid ferric sodium (FeNaEDTA) 36.70 mg/L
- Copper sulfate (CuSO4 · 5H2O) 0.025 mg/l
Vitamins and organic compounds per litre
View attachment 18959560
- Myo-Inositol 100 mg/l
- Nicotinic Acid 0.5 mg/l
- Pyridoxine · HCl 0.5 mg/l
- Thiamine · HCl 0.1 mg/l
- Glycine 2 mg/l
- Tryptone 1 g/l (optional)
- Indole Acetic Acid 1-30 mg/l (optional)
- Kinetin 0.04-10 mg/l (optional)
could you imagine getting your clone in a cryo tube used for seeds?
>Best>ibes>>>
acespicoli
Well-known member
Thats a good question im not sure about faster, although if thats you cup of tea there are faster growing sacraments in the cacti family.
San Pedro, Peruvian Torch, etc
... Google Peyote Church....
Best you have a guide for the journey
Maybe your decide not to travel
Either way personal choice >Best>>>ibes
Propagation is immensely increased via numbers.
If you just want to enhance one cacti's growth then graft.
Cactus Squatter
Well-known member
No. Tissue culturing won’t speed up the growth rate. Lophophora grows extremely slowly, the only way to speed it up a little is grafting it to other root stock.
Honestly, the only reason to actually grow it at home would be from a conservation standpoint. To grow enough of it to use takes so long, and requires so much of them that it’s not really worth it to do. It could take 40 years for one to reach roughly 3 inches in size, and it takes several to get you there.
In terms of expansion of plant mass, tissue culture would be advantageous.
PS You can certainly get three inch Lophophora within ten years
PS You can certainly get three inch Lophophora within ten years
In fascist corporate cannabis state, so no local access. Might have to drive a few states away with list of several sellers and inspect in person before buying. These miscreants sell plants that are heavily infected and trim of all the lower growth that is rotting, and has obvious stem cankers rotting right through the stem.
Strainly, mg1brands, and others are sleazebag scammers. Probably selling fake genetics, and counting on them dying before flowering.
Strainly is the worst of the worst. Besides designing site so that you have to contact seller to get any deals offered, and paying by invoice, then strainly says you went around them and can not leave feedback when clones arrive crawling with spider mites or on verge of death from mold, they gave my e-mail to hackers who have sent over 200 malware infected emails, and just had credit card that I rarely use, and used with them had fraudulent purchases.
On RIU website about 4000 posts, mostly negative about strainly padded with BS fake accounts saying they are great, like has happened in this thread also, with new accounts popping up just to post here, and bury the facts.
MG1brands website is as bad as his clones. Total mess with generic about page not even about them.
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Saw on reddit that someone bought 12 from clone bank and 8 came back testing positive for HLVD.
xtsho
Well-known member
I'm not surprised. I don't understand why people feel the need to purchase clones from shady people. 90% of the time the clones are not going to be what's described and are diseased and infested with bugs. The other 10% are not worth what's being charged. Just get a couple packs of seeds and grow some pot without all the drama. You don't need to go through dozens of plants pheno hunting like some think.
Cannabis growers overcomplicate everything. People without any knowledge of Plant Science are expert horticulturists because they got a plant to flower and then they think they know it all. If they knew it all they wouldn't be paying $500 for a clone and then justifying it by claiming it's this and that special pheno of something. A pack of ten seeds of any decent strain is going to produce some decent plants as good as any of these so called "Fire" cuts some scammer is selling on social media.
xtsho
Well-known member
Just buy seeds off of a reputable seed bank. Why in the hell did you bother with strainly in the first place?
Nothing beats a top shelf clone if healthy. People tried backcrossing GG4 for years, and no one beat it for potency, production, and ease of trimming. Seeds are a crap shoot, and will get different phenos. First post I made when starting this thread was this is not a debate about seeds versus clones. Like indoor/outdoor, hydro/dirt, seeds;clones is personal choice, and not worth debating. If you enjoy playing the seed lottery, that is great. I grew GG4 many times and had various results. Would rather have something that won an award or general consensus from people whose opinions I trust, that, that exact genetic is capable of something exceptional.
Forgot to mention what has already been mentioned in thread. Seeds can also have HLVD and Fusarium, plus other diseases mentioned by acespicoli. According to Tumi, 30% chance of getting infected seeds if either parent infected.
Would love to open a reputable clone company, but need clean or somewhat clean cuts. If not completely clean takes a year to produce tissue cultured mother.
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Will check some of them, but buds and roses sent bum cuts. If someone sends crap, and refunds money, most of the time people will not make a negative post. Buds and roses was very professional, wasted a month of my time letting them grow out to see exactly what I knew was going to happen, happen.View attachment 18889951
The Strainbank · 1440 Hotel Cir N #146, San Diego, CA 92108, United States
★★★★★ · Association / Organizationgoo.gl
The other question I had to answer was how to verify on one was is it a legit Trainwreck cut
Clone Group: OG Kush GAL303 (92 Cuts)
Clone Group: Sour Diesel GAL306 (51 Cuts)
Clone Group: Grand Daddy Purple (34 Cuts)
Clone Group: Bubba Kush GAL301 (19 Cuts)
Guess that puts the popular old cuts in order
You may want to email them for details
- Trainwreck
Harborside- Trainwreck
Lux Cultivars- Trainwreck
HPRC- Trainwreck
California Care Collective- PggSac107
Presidential Garden Group Inc.
Harborside and California Care Collective were some of the top sources on my list
CCC it appears has permanently closed
This company is legit definitely quarantine any new plant material you receiveClones
www.breedersdirectseedco.com
Let us know how you make out love to see the pictures
View attachment 18889958
View attachment 18889959
Had to jog my brain a little
>>>best>ibes
