-
ICMag with help from Landrace Warden and The Vault is running a NEW contest in November! You can check it here. Prizes are seeds & forum premium access. Come join in!
Who is our resident Tissue culture master?
- Thread starter grod31
- Start date
Thank you!
It’s always something I’ve wanted to get into. Now that I see others have done it, I know it’s possible.
The DKW idea came from this paper: https://cdnsciencepub.com/doi/10.1139/cjb-2020-0179
This actually worked out well for me, because I didn’t really want to buy MS media and I already had the DKW raw ingredient and vitamins for gamborg vitamins.
I read another paper somewhere that mentioned silicate (6um I think?) improved shoot rooting. I’m not sure where to start on the hormone side. I have tdz, gib, naa, kin, and bap. I was thinking tdz or tdz + gaa for shoot generation. Then perhaps naa + silicate for the shoot rooting, using DKW/gamborg and sucrose as the basal media for both. I still need to get some ppm, so I can minimize contaminates. Any recommendations on rooting? It seems like that is where things can get tricky. I also was spit balling the idea of adding a ppm or two i of nickel to the medium to help with nitrogen metabolism.
I expect to fail a lot, but it will be fun either way!
That's exactly the paper I read ,,
Your on fire ,,,this is where you develop some special potions,,,
It pains me to drop research in open fourm for companies to soak up , im sat in a shit legal climate and just can't do the work on my own to develop this shit properly
Your on fire ,,,this is where you develop some special potions,,,
It pains me to drop research in open fourm for companies to soak up , im sat in a shit legal climate and just can't do the work on my own to develop this shit properly
I recently got prescribed medical cannabis here in the uk,,,the company is https://lyphe.com/ im considering asking for a job,,,back in 2010 i had started a pharmaceutical company but the world wasn't ready,,,medical cannabis was a pipedream at the time,,
The doctor told me to contact the team to look at being a consultant,,,anyone know who's running it??
The doctor told me to contact the team to look at being a consultant,,,anyone know who's running it??
Thanks Rick, that’s an awesome paper! I also completely understand about not wanting to divulge too much, for the exact reasons you stated. I think I have some polyploid GH cheese buds forming (will need to double check the stoma to confirm). If I do, I will start testing out things and keep the group up to date as I go along!
@englishrick et al Greetings!
This stuff is very interesting. Is there a book or site that has the basic info? Or recommended reading and resources? Is one of those hepa hoods required or can a simplified clean box suffice?
Once this is started can these be kept in stasis for storage? If so what environmental conditions are required? I read about artificial seeds wondering the same for that aspect?
The cleaning aspect seems like the place to begin reading. PM me with some reading recommendations! Following along!
This stuff is very interesting. Is there a book or site that has the basic info? Or recommended reading and resources? Is one of those hepa hoods required or can a simplified clean box suffice?
Once this is started can these be kept in stasis for storage? If so what environmental conditions are required? I read about artificial seeds wondering the same for that aspect?
The cleaning aspect seems like the place to begin reading. PM me with some reading recommendations! Following along!
Rico Swazi
Active member
Anyone incorporating activated charcoal in their media?
The paper I posted earlier (post 49) had this on the subject-
Here's a how to MS , White, Gamborg, Heller et al
sharebiology.com
The paper I posted earlier (post 49) had this on the subject-
Here's a how to MS , White, Gamborg, Heller et al
MS media (Murashige – Skoog) composition and preparation
Table Of Contents Introduction The composition of the growing medium is crucial in every experiment containing living organisms. The need for a well-balanced plant medium arose at the beginning of hydroponic cultivation and plant tissue cultures. The first recipes for plant tissue cultures are...
sharebiology.com
How long does it take to get rooted clones this way? I have been doing a great deal of reading this week on some of the basic aspects. Mainly components, media, still air box and kits.
The microcloner kit seems expensive when you can buy the media packets and vials seperately.
The microcloner kit seems expensive when you can buy the media packets and vials seperately.
You can buy everything you need to make media and vita/hormones much cheaper. The salts used in these media are quite cheap. Problem is, unless you wanna make 100L of media, measuring less than a mg of a micro is really tough. That being said, if you make the basal salts for 100l, then mix well, you can just take what you need from the bulk mix. You pay for convenience with the commercial stuff, but you can certainly do it all yourself if you are the tinkering type!
If you look next to the far right jar I'm this picture you can see a tub of activated carbon,,,Anyone incorporating activated charcoal in their media?
The paper I posted earlier (post 49) had this on the subject-
Here's a how to MS , White, Gamborg, Heller et al
MS media (Murashige – Skoog) composition and preparation
Table Of Contents Introduction The composition of the growing medium is crucial in every experiment containing living organisms. The need for a well-balanced plant medium arose at the beginning of hydroponic cultivation and plant tissue cultures. The first recipes for plant tissue cultures are...
I promise il explain everything I've been working on soon as I've got a chunk of money to put into this properly,,,
Attachments
