RollmeoneKenobi
Member
Thank You Sam and Gypsy for this wonderfull plant
-
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question for sam the skunkman on the original haze
- Thread starter bigherb
- Start date
Thank You Sam and Gypsy for this wonderfull plant
I got a real Hazy pheno. Skinney as hell and tall. It has terps and odor
Ok I moved it outside. It got rained on but looks very happy. it has red circular stripes on the stems. real cool looking.
H
Heathinv
"Orginal Haze" is claimed to be StSM's creation.
the "real haze", is what you ask for and is anybody's guess what you mean.
what he said
Or if youve smoked too much Haze, and say " Im in a real Haze today"
RollmeoneKenobi
Member
Sam could you give me your thought on this please.
Originally Posted by ScienceDaily
A reliable method for producing plants that carry genetic material from only one of their parents has been discovered by plant biologists at UC Davis. The technique, to be published March 25 in the journal Nature, could dramatically speed up the breeding of crop plants for desirable traits.
The discovery came out of a chance observation in the lab that could easily have been written off as an error.
"We were doing completely 'blue skies' research, and we discovered something that is immediately useful," said Simon Chan, assistant professor of plant biology at UC Davis and co-author on the paper.
Like most organisms that reproduce through sex, plants have paired chromosomes, with each parent contributing one chromosome to each pair. Plants and animals with paired chromosomes are called diploid. Their eggs and sperm are haploid, containing only one chromosome from each pair.
Plant breeders want to produce plants that are homozygous -- that carry the same trait on both chromosomes. When such plants are bred, they will pass the trait, such as pest resistance, fruit flavor or drought tolerance, to all of their offspring. But to achieve this, plants usually have to be inbred for several generations to make a plant that will "breed true."
The idea of making a haploid plant with chromosomes from only one parent has been around for decades, Chan said. Haploid plants are immediately homozygous, because they contain only one version of every gene. This produces true-breeding lines instantly, cutting out generations of inbreeding.
Existing techniques to make haploid plants are complicated, require expensive tissue culture and finicky growing conditions for different varieties, and only work with some crop species or varieties. The new method discovered by Chan and postdoctoral scholar Ravi Maruthachalam should work in any plant and does not require tissue culture.
Ravi and Chan were studying a protein called CENH3 in the laboratory plant Arabidopsis thaliana. CENH3 belongs to a group of proteins called histones, which package DNA into chromosomes. Among the histones, CENH3 is found only in the centromere, the part of the chromosome that controls how it is passed to the next generation.
When cells divide, microscopic fibers spread from each end of the cell and attach at the centromeres, then pull the chromosomes apart into new cells. That makes CENH3 essential for life.
Ravi had prepared a modified version of CENH3 tagged with a fluorescent protein, and was trying to breed the genetically modified plants with regular Arabidopsis. According to theory, the cross should have produced offspring containing one mutant gene (from the mother) and one normal gene (from the father). Instead, he got only plants with the normal gene.
"At first we threw them away," Chan said. Then it happened again.
Ravi, who has a master's degree in plant breeding, looked at the plants again and realized that the offspring had only five chromosomes instead of 10, and all from the same parent.
The plants appear to have gone through a process called genome elimination, Chan said. When plants from two different but related species are bred, chromosomes from one of the parents are sometimes eliminated.
Genome elimination is already used to make haploid plants in a few species such as maize and barley. But the new method should be much more widely applicable, Ravi said, because unlike the process for maize and barley, its molecular basis is firmly understood.
"We should be able to create haploid-inducing lines in any crop plant," Ravi said. Once the haploid-inducing lines are created, the technique is easy to use and requires no tissue culture -- breeders could start with seeds. The method would also be useful for scientists trying to study genes in plants, by making it faster to breed genetically pure lines.
After eliminating half the chromosomes, Chan and Ravi had to stimulate the plants to double their remaining chromosomes so that they would have the correct diploid number. Plants with the haploid number of chromosomes are sterile.
The research also casts some interesting light on how species form in plants. CENH3 plays the same crucial role in cell division in all plants and animals. Usually, such important genes are highly conserved -- their DNA is very similar from yeast to whales. But instead, CENH3 is among the fastest-evolving sequences in the genome.
"It may be that centromere differences create barriers to breeding between species," Chan said. Ravi and Chan plan to test this idea by crossing closely-related species.
The work was supported by a grant from the Hellman Family Foundation.
http://www.sciencedaily.com/releases/2010/03/100324142012.htm
Originally Posted by ScienceDaily
A reliable method for producing plants that carry genetic material from only one of their parents has been discovered by plant biologists at UC Davis. The technique, to be published March 25 in the journal Nature, could dramatically speed up the breeding of crop plants for desirable traits.
The discovery came out of a chance observation in the lab that could easily have been written off as an error.
"We were doing completely 'blue skies' research, and we discovered something that is immediately useful," said Simon Chan, assistant professor of plant biology at UC Davis and co-author on the paper.
Like most organisms that reproduce through sex, plants have paired chromosomes, with each parent contributing one chromosome to each pair. Plants and animals with paired chromosomes are called diploid. Their eggs and sperm are haploid, containing only one chromosome from each pair.
Plant breeders want to produce plants that are homozygous -- that carry the same trait on both chromosomes. When such plants are bred, they will pass the trait, such as pest resistance, fruit flavor or drought tolerance, to all of their offspring. But to achieve this, plants usually have to be inbred for several generations to make a plant that will "breed true."
The idea of making a haploid plant with chromosomes from only one parent has been around for decades, Chan said. Haploid plants are immediately homozygous, because they contain only one version of every gene. This produces true-breeding lines instantly, cutting out generations of inbreeding.
Existing techniques to make haploid plants are complicated, require expensive tissue culture and finicky growing conditions for different varieties, and only work with some crop species or varieties. The new method discovered by Chan and postdoctoral scholar Ravi Maruthachalam should work in any plant and does not require tissue culture.
Ravi and Chan were studying a protein called CENH3 in the laboratory plant Arabidopsis thaliana. CENH3 belongs to a group of proteins called histones, which package DNA into chromosomes. Among the histones, CENH3 is found only in the centromere, the part of the chromosome that controls how it is passed to the next generation.
When cells divide, microscopic fibers spread from each end of the cell and attach at the centromeres, then pull the chromosomes apart into new cells. That makes CENH3 essential for life.
Ravi had prepared a modified version of CENH3 tagged with a fluorescent protein, and was trying to breed the genetically modified plants with regular Arabidopsis. According to theory, the cross should have produced offspring containing one mutant gene (from the mother) and one normal gene (from the father). Instead, he got only plants with the normal gene.
"At first we threw them away," Chan said. Then it happened again.
Ravi, who has a master's degree in plant breeding, looked at the plants again and realized that the offspring had only five chromosomes instead of 10, and all from the same parent.
The plants appear to have gone through a process called genome elimination, Chan said. When plants from two different but related species are bred, chromosomes from one of the parents are sometimes eliminated.
Genome elimination is already used to make haploid plants in a few species such as maize and barley. But the new method should be much more widely applicable, Ravi said, because unlike the process for maize and barley, its molecular basis is firmly understood.
"We should be able to create haploid-inducing lines in any crop plant," Ravi said. Once the haploid-inducing lines are created, the technique is easy to use and requires no tissue culture -- breeders could start with seeds. The method would also be useful for scientists trying to study genes in plants, by making it faster to breed genetically pure lines.
After eliminating half the chromosomes, Chan and Ravi had to stimulate the plants to double their remaining chromosomes so that they would have the correct diploid number. Plants with the haploid number of chromosomes are sterile.
The research also casts some interesting light on how species form in plants. CENH3 plays the same crucial role in cell division in all plants and animals. Usually, such important genes are highly conserved -- their DNA is very similar from yeast to whales. But instead, CENH3 is among the fastest-evolving sequences in the genome.
"It may be that centromere differences create barriers to breeding between species," Chan said. Ravi and Chan plan to test this idea by crossing closely-related species.
The work was supported by a grant from the Hellman Family Foundation.
http://www.sciencedaily.com/releases/2010/03/100324142012.htm
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That's cool.
Hey folks, I just found the SKUNKMAN, look for yourself.
He's riding a horse.
http://www.youtube.com/watch?v=eEaizuryPu0
Hey folks, I just found the SKUNKMAN, look for yourself.
He's riding a horse.
http://www.youtube.com/watch?v=eEaizuryPu0
It may interest you Hammerhead.
Here's a way to make plants from
only a piece of fresh stalk.
http://www.icmag.com/ic/showthread.php?t=166408
jyme
Member
thanks dave for the advice.i havent seen them yet i guess i just dint look hard enough.but im having second thoughts now. the original haze x skunk1 i recived as freebies had me all pumped up and have let me down its been almost 7 days and no life yet.im not able to get them to germinate.i dont know why ive always had high rates with all my seeds.i put five in a shop towl and five in the dirt with a heating mat to maintain a constan 70f and still nothing.i was wandering what have others ran into with these strains?are they slow at germination or what.there solid beans i always give one or two of my beans a nice squeeze before i plant them to check for weakness.can some one give me some feed back.
montehierba
Member
DANK HAZE THREAD
DANK HAZE THREAD
BEAUTIFUL THREAD
DANK HAZE THREAD
BEAUTIFUL THREAD
i believe som low germ rates have been reported but many have also had success with recent germanations of that stock
i suggest if you havnt already maybe try soaking a few seeds ,it might help softening the outer shell.but be patient im sure somthing should sprout
1luvbigherb
i suggest if you havnt already maybe try soaking a few seeds ,it might help softening the outer shell.but be patient im sure somthing should sprout
1luvbigherb
So what does that tell us about selfing?
That's why I was hoping Sam would chime in if this holds true the time savings what be tremendous you would never have to inbreed.
tedlarrrge
Member
YO SKUNKMAN!! im in cali, but ive got kin in the midwest...Where for over twenty years, a parent plant of your Skunk #1 still produces the most road kill, stable yielding ass kind ever.... Im a veteran and snob to quality, i see skunks so mis advertised and represented, that it drives me nuts on the west coast...However, i spent alot of time on seedbay, and among many of my freebies i got blessed with from orders.. are five packs of your.... T*skunk Haze, and the south african durban poison X skunk #1...... any insight you have on either of those two varieties i would gladly take and hold onto.... while the Kush craze rages on, diezel and purps i do enjoy in abundance.... sometimes i just feel like turning the clock back with some good ol KIND BUD!!! much love and respect for the work you have done for and to the game over the decades!!! T LARRRGE
