Meristem Culture for a brighter tomorrow

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Meristem culture is a lab technique that involves culturing plant tissues to produce disease-free plants and propagate them quickly. It's a modern method of plant cloning that uses actively dividing plant tissues called meristems.


Here are some steps in the meristem culture process:

1. Excise the meristem: Remove a small piece of the growing tip of a plant's shoot or root, which contains meristematic cells.
2. Sterilize: Surface sterilize the excised piece.
3. Culture: Place the meristem in a liquid or solid culture medium.
4. Regenerate: Grow the meristem into a plantlet, then divide it and add plant hormones to regenerate more plants.

Meristem culture has many benefits, including:

• Eliminating viruses and parasites: Meristems are the only plant tissues that can be free of viruses from an infected plant.
• Preserving germplasm: Meristem culture can preserve germplasm characters.

Murashige and Skoog medium - Wikipedia

en.wikipedia.org

Im going to thoroughly explore this topic and hopefully share some success stories with you!
Hope you enjoy the ride and find some usefulness in this thread as well

 

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The good, we can get a single healthy plant x-times infinity

 

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References​

1. Nehra N.S., Kartha K.K. (1994) Meristem and Shoot Tip Culture: Requirements and Applications. In: Vasil I.K., Thorpe T.A. (eds) Plant Cell and Tissue Culture. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2681-8_3.
2. Varveri, C., Maliogka, V. I., & Kapari-Isaia, T. (2015). Principles for Supplying Virus-Tested Material. Control of Plant Virus Diseases - Vegetatively-Propagated Crops, 1–32. doi:10.1016/bs.aivir.2014.10.004.
3. Bhatia, S., & Sharma, K. (2015). Micropropagation. Modern Applications of Plant Biotechnology in Pharmaceutical Sciences, 361–368. doi:10.1016/b978-0-12-802221-4.00011-x.
4. Spangenberg, G., Wang, Z.-Y., & Potrykus, I. (1998). Meristem Culture. Biotechnology in Forage and Turf Grass Improvement, 7–17. doi:10.1007/978-3-642-72051-2_2
5. https://byjus.com/biology/meristematic-tissue/
6. https://www.slideshare.net/sumanrawte/meristem-cul...