LAB CULTURE: {Lactic Acid Bacteria} How to Guide.

[Maple_Flail]

Thanks for that link, it's invaluable for LAB, the recipes especially.

..... one thing is important and since you're not using sterile technique yer gonna want to wash your hands well and then wash them again to prevent contamination of you LAB culture. No need to be growing anything that you don't want that will mess up or take over your culture or potentially harm you.

Any metal type transfer instrument you use (knife, spoon, etc. ) can be heat sterilized by running it through an open flame.

One thing I noticed on that site was a pic's of LAB being sprayed on lemon/lime tree leaves and fruit, presumably to treat Powdery Mildew and in the report that stashpot posted it noted:

Over-application of LAB Culture to fruit crops [foliar spray to leaves] may result in the loss of sweetness (lowered brix) of fruits. Apply sparingly in the latter stages of the fruiting season.

Does anyone have any thoughts/experience as to how this would affect the finished cannabis flower if sprayed directly on or as residue left on leaves?

well, its a living bacteria that eats sugars to survive. seeing as alot bud boosters for finishing revolve around sugars and carbs I would see over application near the end of bloom as a potential negative.

however I've haven't got a clue where that over-application line would be for pot.

 

[Radicle Rye]

Thanks for posting all the info Stashpot. I just drenched my outdoor smart pots with LABs last week. Trying to get the living soil ready for the upcoming season. I am looking forward to using this as a foliar spray during flower as a mold preventive. This is my first year using LABs, but I was thinking at a minimum one spray during early flower to culture the inside of the nugs with the beneficial microbes. From there on out just using as spot treatment for mold flare ups and rainy weather periods? How are others using LABs for flower foliar feeding? Again, thanks everyone for sharing!!!

 

[CosmicGiggle]

but .... can LAB get you high???

I've got my free, KISS LAB culture going but in the meantime continue to do research online on the different members of the Lactobacillus family, what they do in the human body and how they interact with each other.

It looks like they can modulate our perception of pain in a positive way by influencing the opioid and cannabinoid receptors in our gut/brain!

A simple Google search of lactobacillus and cannabinoid receptors in the stomach brings up lots of research, (mainly on L. acidophilus) some easy to understand and some not so easy!

High and Mighty? Cannabinoids and the microbiome in pain

Abstract


Within the human gut, we each harbour a unique ecosystem represented by trillions of microbes that contribute to our health and wellbeing. These gut microbiota form part of a complex network termed the microbiota-gut-brain axis along with the enteric nervous system, sympathetic and parasympathetic divisions of the autonomic nervous system, and neuroendocrine and neuroimmune components of the central nervous system. Through endocrine, immune and neuropeptide/neurotransmitter systems, the microbiota can relay information about health status of the gut. This in turn can profoundly impact neuronal signalling not only in the periphery, but also in the brain itself and thus impact on emotional systems and behavioural responses. This may be true for pain, as the top-down facilitation or inhibition of pain processing occurs at a central level, while ascending afferent nociceptive information from the viscera and systemic areas travel through the periphery and spinal cord to the brain. The endogenous cannabinoid receptors are ubiquitously expressed throughout the gut, periphery and in brain regions associated with pain responding, and represent targets for endogenous and exogenous manipulation. In this review, we will focus on the potential role of the endogenous cannabinoids in modulating microbiota-driven changes in peripheral and central pain processing. We also focus on the overlap in mechanisms whereby commensal gut microbiota and endocannabinoid ligands can regulate inflammation and further aim to exploit our understanding of their role in microbiota-gut-brain axis communication in pain processing.

Keywords: Endocannabinoids; Gut; Inflammation; Microbiota; Pain.

I just love the symbiotic relationship between the Lactobacillus species feeding the cannabis plant and then sneaking into the human body when we ingest the plant, and all the while, us thinking that it was our idea all along - the inter relatedness of all things!!!!

 

[stashpot]

but .... can LAB get you high???

I've got my free, KISS LAB culture going but in the meantime continue to do research online on the different members of the Lactobacillus family, what they do in the human body and how they interact with each other.

It looks like they can modulate our perception of pain in a positive way by influencing the opioid and cannabinoid receptors in our gut/brain!

A simple Google search of lactobacillus and cannabinoid receptors in the stomach brings up lots of research, (mainly on L. acidophilus) some easy to understand and some not so easy!

High and Mighty? Cannabinoids and the microbiome in pain

Abstract


Within the human gut, we each harbour a unique ecosystem represented by trillions of microbes that contribute to our health and wellbeing. These gut microbiota form part of a complex network termed the microbiota-gut-brain axis along with the enteric nervous system, sympathetic and parasympathetic divisions of the autonomic nervous system, and neuroendocrine and neuroimmune components of the central nervous system. Through endocrine, immune and neuropeptide/neurotransmitter systems, the microbiota can relay information about health status of the gut. This in turn can profoundly impact neuronal signalling not only in the periphery, but also in the brain itself and thus impact on emotional systems and behavioural responses. This may be true for pain, as the top-down facilitation or inhibition of pain processing occurs at a central level, while ascending afferent nociceptive information from the viscera and systemic areas travel through the periphery and spinal cord to the brain. The endogenous cannabinoid receptors are ubiquitously expressed throughout the gut, periphery and in brain regions associated with pain responding, and represent targets for endogenous and exogenous manipulation. In this review, we will focus on the potential role of the endogenous cannabinoids in modulating microbiota-driven changes in peripheral and central pain processing. We also focus on the overlap in mechanisms whereby commensal gut microbiota and endocannabinoid ligands can regulate inflammation and further aim to exploit our understanding of their role in microbiota-gut-brain axis communication in pain processing.

Keywords: Endocannabinoids; Gut; Inflammation; Microbiota; Pain.

I just love the symbiotic relationship between the Lactobacillus species feeding the cannabis plant and then sneaking into the human body when we ingest the plant, and all the while, us thinking that it was our idea all along - the inter relatedness of all things!!!!

I actually have the L.acidophilus in tablet form its good has millions of beneficial bacteria, i actually put some in water before and fed them to plants haha! i didn't see any side effects i wonder if they worked similar to the LAB.

great post cosmic interesting read and I'm sure will benefits somebody's health.
I too love the symbolic relationships these plants most likely formed way before humans even got here.


Here's an opinion of mine on cannabis plants relationships with interactions with the stuff we give it and natural occurrences.
The cannabis plant can deny things from getting into it that will make it sick, kill it, etc.
Just like a dog or animal or human when it smells and inspects things before it eats it, in order to figure out if it will poison them or make them sick etc, that's why dogs have great senses they are a survival mechanism, i feel like plants have that same filter in the plant world, they filter out the bad and only take whats good.

If you put something into the water feedings that the plant doesn't like or doesn't benefit from or makes it sick the plant will understand this and try filter and or discard it, or lock itself out in order to survive so its left in the soil at which point needs to be leeched.

That's why if i ever try anything new like the L/acid. on a plant and soil, i'll always do it without food teas or lab or fpj etc etc, so that i can see the effects and if it locks it out.

So yeah i think cannabis has relationships with stuff that we never knew about and probably never will know about.

Like coconuts for example who knew coconuts and the water could turn your plant into a monster then you learn why and it all makes sense. Peace