Doubled haploids

[GMT]

post 97, is that due to the costs, or the practicalities?

 

[Tom Hill]

you have not one but TWO true breeding lines - to be explored across an unlimited hosts of possibilities?

 

[Tom Hill]

you're just pissed you can't do it in your closet....

 

[GMT]

hmmmm, the businessman who leaves the problem of costs to the customers is overlooking the principles of price elasticity ie. its all well and good until you realise you need to charge £500 per 10 seeds and sell 10k of them to recover your costs. (numbers exagerated to prove the train of thought)

 

[Tom Hill]

post 97 was about math, and project management skills

 

[GMT]

I'm not pissed at all, I'm intrigued.
can you explain post 102?

 

[GMT]

you know its all well and good to make general statements, but its like saying, hey I'm patenting a time machine, not sure how it works yet, but that's what the scientists I employ are for.

 

[Tom Hill]

who said anything about customers? not me... many folks want to see how it pans out, for their amusement and the betterment of canna-kind. I'd have no problems keeping all efforts from this type of project, as you do, from that thing you've been doing.

 

[Tom Hill]

nobody is patenting anything, we are exploring, without regard to any of that other, it may be difficult for some to understand, but people actually do it anyway.

 

[Tom Hill]

I know a guy who was convinced that humans first came from Scandinavia, spent all his sinse dough trying to prove it too. It didn't pan out. But if for a second you think that is some hole in my thinking that might give me pause I would politely suggest you think again.

 

[Tom Hill]

post 102 was about the splitting of the heterozgote into two parents, two truly homozygous DH parents, to be used against each other, or to be used in a number of other scenarios as well.

 

[GMT]

ok, I'll take my hat off to ya if you're saying that you are going to use your time and cash to do this, purely for the enjoyment and achievement of doing it. I hope you share some details once you've worked them out. I don't agree with your statement that you create 2 true breeding lines though. If you start with a standard diploid, and try to re-create it, once you have achieved that, given the misgivings I have just stated, I still only see 1 line. Where is the other? Unless you are talking about selfing the 2 newly created P1s. In which case it could be argued 3 lines I guess. But still you don't have any males in the mix, given your insistance that anything bred should be able to survive in the wild, how do you see this situation?


No correction, I'm wrong, the re=created F1 wouldn't be true breeding would it, under any definition, I see only the P1s would be

 

[Tom Hill]

all of your answers you seek re how many homozygous "lines" we have created have been right there in figure 2, the whole time man.. Males? Brother please! lol

 

[Tom Hill]

who have you been talking to?
in the wild? lmao, cannabis is anything but a weed at this point, While I do endevour to broaden its environmental niche, I am not an idiot... The cream off the top has a very narrow range of environment in which it thrives.

 

[Tom Hill]

my wish for it to thrive in the wild is completely and absolutely foreshadowed by reality.

 

[bajangreen]

You 2 ever meet in real life? sounds like you would get along quite nicely!

 

[Stoxx]

First two pages, great info by Mofeta. I'm not following the last page at all, but hello Tom, anyone that knows your work that I have come across loves your work. Which has led to my interest in deep chunk and monkey balls. Keep up the good work.

I also wanted to ask people with good scientific books-- do you have PDF versions and/or links to scribd versions? My background is not in plant botany or plant biochemistry or statistics, but rather chemistry, physics (u. grad), and engineering. I would say what engineering but I would rather not provide information that helps identify myself.

My point being, I probably can pick up a graduate level text and get up to speed very quickly. I also have a few years growing experience and have more recently started my own home biotech experiments, with mixed results.

I'm looking for good resources outside what is easily found via search. Thanks in advance.

Stoxx
CA215/SB420 compliant. Not legal, medical or grow advice.

 

[Tonygreen]

I really believe your misgivings are misplaced. We can do most of this no problem. The real trick is regenerating plantlets from spores.... As those scientists just accomplished with the Siberian permafrost fruit. It's been done, is being done, now it is a matter of protocol to fit. We begin by copying, as usual, and flex as need be. But no, this is by no means out of reach imo. And I am quite willing to bet on it.

This post gave me shivers.

 

[Tom Hill]

My brother Stoxx,

I come from a long line of engineers, math men, back before we felt the need to separate them, you want a rocket, a bridge? Name your poison says the math guy. All of this is simple statistics/probability, a light lunch for a student of calculus where he may be asked to accept that .9999, barred, = one. You sold your age out, thinking type of engineer had relevance, that was just some bs way to spread out your academia into many jobs.

Waded through it? Good, Bos and Caligari's "Selection Methods in Plant Breeding" is my favorite text currently on the market. you'll have to pay to separate yourself from the pack cheap chalie mofo looking for free crap, but hey, then we'll have someone to talk to.

 

[Tonygreen]

Printing that bad boy as we speak.