Ok I'm in.
I'll toss a pack of f2's in a shot glass and see if we can get a worthy male. I have 2 or 3 packs and can get more. Im thinking a male from the f2 would be better would it not?
Pal correct me if im wrong but aren't the parents of the f2's both gg leaners?
Yep thats perfect, just select the closest glue lookin male you find and then go back to the cut. Rinse repeat.
I'll have Gb BX4 waiting to meet ya.
It's a little work but well worth it. More than half the work is already done.
Possibly could be done by about this time next year, 4 runs of DN bxes then we'd be ready to do the RIL.
Recombinant Inbred Line, we're on some serious shit yall.
This paper shows evidence to support that in a 2 way RIL 99% fixation can be achieved in 8 generations complete genome wide fixation can be achieved in 10.5 generations, based on a 10,000 trial computer simulation they did.
http://www.complextrait.org/archive/2005/pdf/rigenome.pdf