http://www.ncbi.nlm.nih.gov/pubmed/23354583
Anal Bioanal Chem. 2013 Jan 27. [Epub ahead of print]
Cloud point extraction of Δ(9)-tetrahydrocannabinol from cannabis resin.
Ameur S, Haddou B, Derriche Z, Canselier JP, Gourdon C.
Source
Faculté des Sciences, Département de Chimie, Laboratoire de Physico Chimie des Matériaux, U. S. T. Oran, BP 1505, M'Nouar, Oran, Algeria.
Abstract
A cloud point extraction coupled with high performance liquid chromatography (HPLC/UV) method was developed for the determination of Δ(9)-tetrahydrocannabinol (THC) in micellar phase. The nonionic surfactant "Dowfax 20B102" was used to extract and pre-concentrate THC from cannabis resin, prior to its determination with a HPLC-UV system (diode array detector) with isocratic elution. The parameters and variables affecting the extraction were investigated. Under optimum conditions (1 wt.% Dowfax 20B102, 1 wt.% Na(2)SO(4), T = 318 K, t = 30 min), this method yielded a quite satisfactory recovery rate (~81 %). The limit of detection was 0.04 μg mL(-1), and the relative standard deviation was less than 2 %. Compared with conventional solid-liquid extraction, this new method avoids the use of volatile organic solvents, therefore is environmentally safer.
Anal Bioanal Chem. 2013 Jan 27. [Epub ahead of print]
Cloud point extraction of Δ(9)-tetrahydrocannabinol from cannabis resin.
Ameur S, Haddou B, Derriche Z, Canselier JP, Gourdon C.
Source
Faculté des Sciences, Département de Chimie, Laboratoire de Physico Chimie des Matériaux, U. S. T. Oran, BP 1505, M'Nouar, Oran, Algeria.
Abstract
A cloud point extraction coupled with high performance liquid chromatography (HPLC/UV) method was developed for the determination of Δ(9)-tetrahydrocannabinol (THC) in micellar phase. The nonionic surfactant "Dowfax 20B102" was used to extract and pre-concentrate THC from cannabis resin, prior to its determination with a HPLC-UV system (diode array detector) with isocratic elution. The parameters and variables affecting the extraction were investigated. Under optimum conditions (1 wt.% Dowfax 20B102, 1 wt.% Na(2)SO(4), T = 318 K, t = 30 min), this method yielded a quite satisfactory recovery rate (~81 %). The limit of detection was 0.04 μg mL(-1), and the relative standard deviation was less than 2 %. Compared with conventional solid-liquid extraction, this new method avoids the use of volatile organic solvents, therefore is environmentally safer.
