Cannabis Seed Storage

[chilliwilli]

@chilliwilli Took me awhile to get back to this think you gave me the info?
Do you have more details
Best>>>

no i can't remember that i gave you that info.
Haven't used it for germination but as a boost in early veg(~20ml/liter). Still have to try it as late flower boost.

 

[acespicoli]

A sustainable approach in agricultural chemistry towards alleviation of plant stress through chitosan and nano-chitosan priming. Discov. Chem. 1, 44 (2024). https://doi.org/10.1007/s44371-024-00046-2

 

[acespicoli]

Optimizing cannabis cultivation: an efficient in vitro system for flowering induction - Plant Methods

Background Cannabis sativa L. is a versatile medicinal plant known for its therapeutic properties, derived from its diverse array of secondary metabolites synthesized primarily in female flower organs. Breeding cannabis is challenging due to its dioecious nature, strict regulatory requirements...

plantmethods.biomedcentral.com

 

[acespicoli]

Results​

Analysis of Cannabis seed endophyte composition and diversity across 46 plant genotypes revealed 813 different bacterial genera with a predominance of Gammaproteobacteria, Bacilli, Actinobacteria and Alphaproteobacteria but a genotype-specific microbiome. The assessment of domestication and breeding on microbial assembly revealed a higher bacterial diversity in low domestication genotypes (Shannon index, H′: 1.21 vs. 1.05) and a higher homogeneity in bacterial composition caused by line development. Further, a seed bacterial isolate (Bacillus frigoritolerans C1141) associated with low domestication genotypes, and with genes associated with bio-fertilization, bioremediation and phytohormone production, increased plant growth by 42.3% at the time of harvest, under field conditions.

Lobato, C., de Freitas, J.M., Habich, D. et al.
Wild again: recovery of a beneficial Cannabis seed endophyte from low domestication genotypes.
Microbiome 12, 239 (2024). https://doi.org/10.1186/s40168-024-01951-5

 

[acespicoli]

First on the graph in the seed fridge you will see the govee sensor left on the shelf
The last part of the graph is the govee sensor reading from inside the double wall insulated stainless steel seed locker

The other is the cheap fridge thermometer in degrees F

Note: We dont want freeze and thaw cycles that destroy seeds we want 36-38F ...
Once frozen do not refreeze ask your pollen chucker and seed jockeys how they were stored...

 

[acespicoli]

During seed storage, metabolism slows down significantly, but not completely, with seeds relying on stored reserves like lipids, carbohydrates, and proteins for energy and building blocks, while environmental factors like temperature and moisture greatly influence seed viability and the rate of deterioration. [1, 2, 3, 4, 5]
Here's a more detailed explanation:
Metabolic Processes in Seed Storage: [1, 3]

• Reduced Metabolism: Seeds in storage are in a state of metabolic dormancy, meaning their metabolic activity is greatly reduced compared to germinating or growing seeds. [1, 3]
• Storage Reserves: Seeds store various compounds as reserves, including: [4, 6]
• Lipids: Primarily in the form of triacylglycerols (TAGs). [4, 6]
• Carbohydrates: Including starch and other sugars. [4, 7]
• Proteins: Including seed storage proteins (SSPs). [8, 9, 10]

• Metabolic Activity During Storage: [2, 3, 9]
• Minimal Activity: While metabolism is reduced, some metabolic processes continue, including those related to antioxidant defense and repair mechanisms. [2, 3, 9]
• Deterioration: Under unfavorable storage conditions (high temperature, moisture, etc.), deterioration processes accelerate, leading to loss of viability. [1, 2, 3]
• Reactive Oxygen Species (ROS): During storage, ROS, such as superoxide and hydrogen peroxide, can be generated, causing damage to seed components. [2]
• Antioxidant Defense: Seeds possess antioxidant systems (enzymatic and non-enzymatic) to protect themselves against ROS damage. [2]
• Seed Storage Proteins (SSPs): Some SSPs play a role in seed longevity, potentially buffering seeds from oxidative stress. [9]

• Seed Storage Oil Mobilization: During germination, stored oil reserves are mobilized, with TAGs being broken down into fatty acids, which are then converted to 4-carbon compounds that provide energy for seedling growth. [4, 6]

Factors Affecting Seed Storage: [1, 5]

• Temperature: Lower temperatures slow down metabolic processes and reduce deterioration. [1, 5]
• Moisture: Dry conditions are crucial for long-term storage, as high moisture content promotes fungal growth and deterioration. [1, 5, 11]
• Oxygen: While seeds need oxygen for respiration, excessive oxygen can lead to oxidative stress. [1, 2]
• Storage Containers: Using airtight containers helps prevent moisture uptake and maintain seed viability. [11, 12]

Generative AI is experimental.

[1] https://onlinelibrary.wiley.com/doi/10.1111/rec.13174[2] https://www.scielo.br/j/brag/a/C5PvJHYNV4PhZMXGB4d93vw/[3] https://pmc.ncbi.nlm.nih.gov/articles/PMC8869448/[4] https://www.sciencedirect.com/science/article/pii/S2773126X22000405[5] https://www.starkeayres.com/news/a-guide-to-seed-storage-and-maintenance-for-farmers[6] https://pubmed.ncbi.nlm.nih.gov/18444898/[7] https://www.intechopen.com/chapters/56975[8] https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/seed-storage-protein[9] https://pubmed.ncbi.nlm.nih.gov/26184996/[10] https://pubmed.ncbi.nlm.nih.gov/21614590/[11] https://www.southernexposure.com/notes-on-seed-storage/[12]

 

[acespicoli]

• Oxygen: While seeds need oxygen for respiration, excessive oxygen can lead to oxidative stress. [1, 2]


Seeds are mini alive seeds laying in a state of semi dormancy sipping on reserves till they hit the right conditions or empty
Seeds love % viabilty every year
Year one 100% germ
Year five 10-20% viable
Year five - ten maybe 10% or less you need alot of seeds for room temp storage

Fridge is much better for longevity it slows the metabolism down considerably

 

[Osama Bong Loadin']

MjPassion, lol You find any rez v3 in there?

What are the oldest seeds you have germinated and what % grew ?
Just interested because like you said, its not difficult to save seed.
Oldest I have germinated are probably 7 years old.

100% germination last fall on 21+. year old seeds. Keep in jars with rice in a fridge. Had to move them twice last year. I hope they are ok. I don't always get 100%. I hope my next ones do as well.

 

[acespicoli]

sensor temp and humidity in two states one
outside insulated container
other last two days inside insulated container

*for comparison and recommendation purposes

100% germination last fall on 21+. year old seeds. Keep in jars with rice in a fridge. Had to move them twice last year. I hope they are ok. I don't always get 100%. I hope my next ones do as well.

refrigerated you should be good frozen id go to fridge not back to freezer if there was a hiccup

Todd & Mel Frank (not too bad for the wear?)

Hope thats been inspiring and helpful

 

[Osama Bong Loadin']

Heck pot seeds have lived through at least a 100 million ice ages, where most places on earth are covered over by huge glaciers that take roughly 40,000 - 70,000 years to melt so they can survive pretty much anywhere. But because I know that one thing I also know that any dark and cold place is the perfect place to store all your best pot seeds. Heck, I store all my pot seeds in those old screw-on 35MM film canisters in the cellar of my old house built in 1749, and I pop 30+ year old pot seeds that are stored in that cellar without much troubles all the time!!! Sure I don't always get 100%, but there is no such thing as 100% anything, so if (1) don't pop I germinate another one... It sort of like that Jimmi song... if that baby don't love me no more,
I know her sister will, LoL!

I use the old film containers too. I never had 100% germination on old seeds before. I am worried moving them around started a degradation process. They definitely were exposed to warmer temps several times while in transit. I never open the jars unless I have to.

 

[acespicoli]

Front. Nutr. , 06 June 2021

Sec. Food Chemistry

Volume 8 - 2021 | https://doi.org/10.3389/fnut.2021.678421

Identification and Characterization of the Seed Storage Proteins and Related Genes of Cannabis sativa L.

I use the old film containers too. I never had 100% germination on old seeds before. I am worried moving them around started a degradation process. They definitely were exposed to warmer temps several times while in transit. I never open the jars unless I have to.

Had mine in a storage unit for awhile during a move... it was the most secure option at the time...wide temp swings (they made it) summer winter
Buried in the ground below your local frost line is a great option

Your seeds should be fine tho

 

[acespicoli]

Temperature and Moisture Content for Storage Maintenance of Germination Capacity of Seeds of Industrial Hemp, Marijuana, and Ditchweed Forms of Cannabis sativa
Ernest Small &Brenda Brookes
Pages 240-255 | Published online: 11 Dec 2012
Cite this article https://doi.org/10.1080/15440478.2012.737179
Oct 22, 2024 — Storing seed with a moisture content of 11% at 20 °C reduced germinability of hemp seed to zero in less than 18 months (Small and Brookes 2012) ...

Abstract
Cannabis sativa seeds of three industrial hemp cultivars, a medicinal strain of marijuana, and a ruderal strain were subjected to combinations of four temperatures (20°C, 5°C, −20°C, and −80°C) and three seed moisture contents (approximately 11%, 6%, and 4%) for 66 months. Storage of seeds with a moisture content of 11% at 20°C reduced the germinability of seeds of all varieties to zero in less than 18 months. Either reducing the temperature to at least 5°C or reducing the seed moisture content to at least 6% had a huge beneficial effect on maintaining seed viability. Additional reduction of temperature, but not additional reduction of moisture content had a small supplementary beneficial effect. No apparent benefit was noticed from oxygen-free seed storage.

 

[@hempy]

Seed stored in Mylar seed packs that are air./moisture/lightproof that you seal up with a hot iron is a cheap and a very affective way to preserve seed long term.

I put them in a container and seal that with tape as an extra barrier.

Store in the fridge at around 3c to 4c, seed will remain viable for up to 20 years.

I have germinated seed that was 23/24 years old, but that's pushing it, you should also check the viability of the seed after a set amount of years as the germ % will lessen over time.

 

[acespicoli]

View attachment 19166507

Seed stored in Mylar seed packs that are air./moisture/lightproof that you seal up with a hot iron is a cheap and a very affective way to preserve seed long term.

I put them in a container and seal that with tape as an extra barrier.

Store in the fridge at around 3c to 4c, seed will remain viable for up to 20 years.

I have germinated seed that was 23/24 years old, but that's pushing it, you should also check the viability of the seed after a set amount of years as the germ % will lessen over time.

I have germinated seed that was 23/24 years old, but that's pushing it,
Thats AMAZING and inspiring

Agree 10 years is a good repro point under fridge
Thanks for sharing your knowledge @HEMPY