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A Guide to Fishsticks

Dr. Purpur

Dr. Purpur

Custom Haze crosses
Veteran
My older son is stuck in Oregon pressing over 100 lbs of bud and trim. He couldn't break away but sent the trim crew. They just arrived from Oregon. They are going to work here and the grow house
 
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unregistered190

Senior
Veteran
I remembered seeing Turkey Tails in the woods close by a couple years ago, I think I posted a pic in Waxi's thread even for identification.

So now we have been speaking of mushrooms I decided to take the dogs for a walk to that area and picked a few to bring home.

After reading up on them I believe I have the False Turkey Tail and not the desired Trametes versicolor.

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unclefishstick

unclefishstick

Fancy Janitor
ICMag Donor
Veteran
be very cautious about collecting wild mushrooms...learn to make spore prints and have a good reference book...




mornin campers...


raining and grey today...i guess this is proper fall weather for most people...makes me want to eat soup and take a nap...i'm still waiting for nm fall weather...cool and bone dry...


gotta go on a grocery run....i have monkey butt over for a couple of days so normally i just do house work while she's here and keep her entertained between her numerous daily naps...
 
I

Ichabod Crane

Well-known member
Veteran
unclefishstick said:
be very cautious about collecting wild mushrooms...learn to make spore prints and have a good reference book...




mornin campers...


raining and grey today...i guess this is proper fall weather for most people...makes me want to eat soup and take a nap...i'm still waiting for nm fall weather...cool and bone dry...


gotta go on a grocery run....i have monkey butt over for a couple of days so normally i just do house work while she's here and keep her entertained between her numerous daily naps...
Click to expand...

Just about fall weather here to. 41 degrees right now. Almost time to let my back hair down.
 
Hammerhead

Hammerhead

Disabled Farmer
ICMag Donor
Veteran
Vanilla Phoenix said:
Been trying to germ some old seeds using a paper towel. No luck in getting them to pop. Anyone got a good method to pop old seeds?
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If the seeds are still viable you can help getting them going. Soak the seeds for 36 hrs. If no tap root has cracked the shell you will need to do that for it. I use my teeth it's the easiest way. Make sure you crack the pointy end not the flat end where it was attached to the plant. If its viable it will grow.
 
unclefishstick

unclefishstick

Fancy Janitor
ICMag Donor
Veteran
:badday: doesn't look like it's going to stop raining anytime soon...best get rolling to the grocery store though,got garden chores again today...


we need the rain,only up to 6 inches for the whole year,that's slim even for here...
 
O

[omerta]

Vanilla Phoenix said:
Been trying to germ some old seeds using a paper towel. No luck in getting them to pop. Anyone got a good method to pop old seeds?
Click to expand...


This can help.

Originally Posted by Chimera, poping old seeds
The protocol I use is pretty simple.

5% bleach in autoclaved, distilled water with a couple of drops of Tween-20 per liter of solution. I sterilize the water in Duran jars (loose lids) in a pressure cooker @ 20 PSI for 20 minutes, and then allow the jars to cool under my flow hood and tighten the lids when the water has cooled. This is probably overkill for most purposes, but it's a variation of the protocol I use for sterilizing explants for tissue culture, and it seems to work with minimal contamination. You can get away with a drop of dish detergent instead of the Tween, I just have the tween-20 in the lab so I use it. If you are going to be doing a lot, you can order a few ml of Tween-20 on Amazon if you like.

First I rinse the seeds in a tight mesh strainer to remove any leaf debris that might be harboring bacteria or spores. These seeds were a little dirtier than most having been field grown, so you definitely want to wash away any superfluous materials. This can be up to a 5 minute rinse in lukewarm water. Cannabis achenes have a lot of little crevices where spores and bacteria can hide, so a thorough rinse is a wise step.

Then I take the seeds and drop them in maybe 100-200 mls of the bleach/Tween-20 solution in an Erlenmeyer flask, but any clean glass vessel will do -the seeds should be fully immersed, maybe 2x the volume of water as seed. You want to keep stirring and swishing the seeds around gently to ensure the current is washing away any contaminants. After a minute of swishing I remove the bleach and strain the seeds from the liquid and start again. No more than 3 minutes total, and if I see bleaching on the small test batch (always do a test batch to seed how the seeds react), sometimes I'll only do 2 minutes.

The next part is key. Immediately rinse 3-5 times (for 5 minutes per rinse) in the same sterilized distilled water (baby water / distilled water from Walmart or the local pharmacy is fine), again using a tight mesh strainer, or simply rinse the seeds in a different vessel with the same volume of water, at least 3 times to ensure the bleach is removed. I use a labratory orbita shaker, but you can do it by hand it's just a little tedious. Once the rinses are completed, soak the seeds in the distilled water as before, for 2-24 hours depending on the age of the seed and the medium you will be growing in. Sometimes I'll use agar plates, but most often I'll just sprout them in paper towels and transfer to a light dirt (like Light Warrior for you Americans) or surface sow on a .peat based planting substrate. You can use whatever medium you prefer, it's not that big of a deal as long as the seeds remain moist.
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or you can use something like this:

seedbooster-plus-10ml-plagron-1-1.jpg


seedbooster-plus-10ml-plagron-4-1.jpg
 
Grizz

Grizz

Active member
Veteran
Hey you yards I was talking with Joe and we are setting up a seed auction for funds for Dan. If you want to help contact Joe or me. Probably thru GLG and IG
 
unclefishstick

unclefishstick

Fancy Janitor
ICMag Donor
Veteran
been meaning to go pheno hunting again in the gorilla berry...the c99 have all basically been clones of one another in the 30 or so i have popped...
 
Dr. Purpur

Dr. Purpur

Custom Haze crosses
Veteran
unclefishstick said:
packed in rice and silica gel packs in the fridge in a sealed container...
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Those are likely still alive.

There is nothing quite like strain hunting, and doing crosses. Most of my current run is a search right now. Everything is cloned, and in the house. Lots of new stuff this year.

Some company sent me free seeds labeled GG4. I had a good laugh. I didn't pop them
 
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