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Increase your trichome production, without stressing your plants

Hundred Gram Oz

Our Work is Never Over
Veteran
We have heard tons of stories on how to produce more trichomes ranging from watering your plants with freezing cold water a few days before harvest, to stripping all of the leaves from your plants a week before harvest...what do all these crazy sounding methods mean/do? Well they all go on the basic principle that when a plant is under attack it will defend itself by whatever defence mechanism it holds, cannabis uses its resin glands for defence so the theory is that when you attack your cannabis plants they will defend themselves by producing more trichomes, does this work? Even if it did would the plants still produce more THC to these new resin glands or regulate it through all the new trichomes? Well I don't know, all I can do is give out the information for those that have the time to test these methods

Once a plant is under attack by bugs and pests it produces a chemical known as 'jasmonate' this chemical is produced by the plant that is under attack, it is a warning chemical, it warns all plants in the area that it is under attack so they have time to put up their defences. Jasmonate isn't just produced by cannabis plants, it's produced by all plants. If you think of it like this, when you experience pain, pain is a chemical in your head that is released and you feel the sensation of pain, with this pain you know that you have a problem so you can take it easy for a while not to do anymore damage. Well jasmonate works in the same principal, its a warning chemical only in this case it's produced by one plant into the air to warn all plants in the area that there are attackers nearby.

What if there was a way to get the substance jasmonate into your growroom, you could get your plants to go into defensive mode without hacking them to bits, pouring freezing cold water on them, or putting them in darkness for prolonged periods of time, etc yes these methods will put your plants into defensive mode but they will also stress your plants out to the max! Which will slow growth, give the opportunity of hermaphrodites, kill your plants and so on.

Jasmonate can be bought but it is very expensive, many of our cosmetic products hold Jasmonate, if a way of exposing our plants to jasmonate is found, it would need to be efficient or the ends would not justify the means, after all were trying to produce more thc by being as efficient as we can. An aromatherapy method would be a good way to go.

Here is an article that was written by the laboratory news that you should look at:

UPTON, NY - Scientists at the U.S. Department of Energy's Brookhaven National Laboratory have adapted radiotracer tools and imaging techniques pioneered at Brookhaven for medical science, exploiting the technology to discover new aspects of the way plants respond to stresses such as environmental pollutants, microorganisms, or grazing insects.

Using radioactively "tagged" molecules and sensitive detectors to produce high-resolution images, the scientists demonstrate that jasmonate, a hormone produced by plants in response to stress, moves quickly throughout the plant. This provides evidence that jasmonate may be responsible for "broadcasting" an "attack" warning to trigger widespread defensive action. The studies, which have been published by the journal Planta, also demonstrate that jasmonate affects the movement of sugar, the basic commodity for plant growth.
"It's like an adrenaline rush," said Brookhaven plant scientist Richard Ferrieri, corresponding author on the paper, suggesting that the jasmonate-triggered rush of sugar may help fuel the plant's defensive response.
The scientists were particularly impressed with the detail of the images, which allowed them to discern directional movement within the plant's vascular system. "We were able to 'see' jasmonate move within both the phloem, which delivers sugars from the leaves to places where it is used for storage or growth, and the xylem, which delivers water and mineral nutrients from the soil. The findings could have major ecological and evolutionary implications for our broad understanding of signaling in plants," he said.
Scientists have known for some time that, after one part of a plant is damaged, defensive responses soon occur in other regions, showing that information of the attack is somehow transmitted to warn other regions to be on the defence. Jasmonate has been implicated in signaling this information.
To examine the role of this hormone, the Brookhaven scientists developed a method to "label" jasmonate with a radioactive form of carbon (carbon-11), so that its location and movement within a plant can be "imaged" using a technique known as positron autoradiography. This approach for plant studies grew out of basic radiochemistry tools developed at Brookhaven for imaging the movement of drugs and other substances in humans and animals using positron emission tomography (PET), a technique now commonly used in medical diagnosis and research.


Two physical properties of carbon-11 make this method attractive for such studies. First, it emits radiation that can be detected non-invasively in living organisms, so the plants can be studied in their fully functional condition. Second, the radioactivity lasts for only a short time - it has a half-life of about 20 minutes. So the plants are not radioactive after an experiment, and can therefore be tested again and again. Previously it was only possible to measure radioactivity in a plant if it was cut into small pieces and dried, therefore destroying the organism. This severely restricted opportunities to observe dynamic metabolic processes while they are operating within plants.
As the current research demonstrates, this is not the case using short-lived radiotracers as imaging biomarkers for plant studies. Using autoradiography to take snapshots in time, the Brookhaven scientists were able to observe the flow of radioactive jasmonate throughout the plant's vascular transport system.
"The ability of jasmonate to rapidly exchange between both xylem and phloem, enabling it to reach places within the whole plant that would not be accessible if only one system were involved, provides significant evidence to support its role as a general 'attack' signaler, and may have further implications for understanding plant signaling in general," Ferrieri said.
As part of the study, the scientists also tracked the movement of radiolabeled sugar, produced by plants as a product of photosynthesis, using the same radioactive isotope, carbon-11. This technique has been used for plant science at only three other laboratories worldwide. Using radiolabeled sucrose, scientists can study the effects of jasmonate and of pharmaceuticals that change specific processes within experimental plants. In the Brookhaven study, jasmonate applied to certain focal leaves increased the active loading of sugar into the vasculature of those tissues, as well as the movement of sugar to all parts of the plant.
"We believe that jasmonate enables more chemical energy to become available to the plant's transport-system, resulting in more sugar delivered to other parts of the organism that are acting defensively," Ferrieri said.
Extending beyond the current study, these new, non-invasive imaging techniques have greatly advanced scientists' understanding of how plants respond to environmental stresses, and have many potential applications. "Obviously, the techniques could have significant implications for improving agriculture in this country," Ferrieri said. "But we also see opportunities for improving plant performance in other roles: for example, in using plants to clean up environmental pollutants, a process known as phytoremediation, or improving the production of biofuels from crops to enhance this country's future sustainability and energy security."
This research was funded by a Laboratory Directed Research and Development grant, by the Office of Biological and Environmental Research within the U.S. Department of Energy's Office of Science, and by the German Academic Exchange Service.

Link
http://www.bnl.gov/bnlweb/pubaf/pr/PR_display.asp?prID=07-29

So there we have it now lets discuss...
 

Texicannibus

noob
Veteran
First thing that comes to mind instead of buying the chemical if the plant uses it to communicate stress... at the least could only use your stressing technique on one plant and let it tell the others ... just a idea
 

Hundred Gram Oz

Our Work is Never Over
Veteran
First thing that comes to mind instead of buying the chemical if the plant uses it to communicate stress... at the least could only use your stressing technique on one plant and let it tell the others ... just a idea

Thats a great idea Tex, I was thinking about that last night but forgot to enter it in here, thank you Mrs Sativa :D. But what if we could enhance the defencive response by using higher levels of jasmonate than any plant could create? If you think how far we have come in the past 30 years in creating higher yields and shorter growth times thanks to the indoor growing culture and superb beding, it leaves the door right open.
HGO
 

durgamata

Member
If it comes in several plants, find a few plants that dont harm your garden or take up too much space and beat them with a plastic bat every few days.
 

Hundred Gram Oz

Our Work is Never Over
Veteran
If it comes in several plants, find a few plants that dont harm your garden or take up too much space and beat them with a plastic bat every few days.

I can hear them poor little plants scream now, tut tut.... Well I was thinking more in the lines of the aromatherapy method, the candles with jasmonate in them could be burned inside the growroom, only think is that I don't know A.) How much jasmonate is used to trigger a defensive reaction by the plants. And B.) How much jasmonate is in the candles...a little but more study is needed but that's why this thread is here.

HGO
 
There are ways to get jasmonates into your garden. I believe Harpin (sold as Messenger) activates the jasmonic acid pathway. When I was doing research to try to figure out what is in Canna Boost, I think I found some literature about supplementing or triggering jasmonates (specifically jasmonic acid, I believe). I am a bit medicated, so I might have to do the search later :)
 

Texicannibus

noob
Veteran
Is a excellent point that chemically Im sure we could send a stronger signal than a single plant could . Maybe someone will be willing to test the candles out.
 
G

good drown

does it matter where the jasmonates come from(like what kind of plants)
if not, i would imagine one could start a business collecting and extracting the stuff into very potent concentrates, then sell it to hydro stores. do a couple of tradeshows first, and within a year it'd be in stores
 
does it matter where the jasmonates come from(like what kind of plants)
if not, i would imagine one could start a business collecting and extracting the stuff into very potent concentrates, then sell it to hydro stores. do a couple of tradeshows first, and within a year it'd be in stores
Probably not, but you never know. It's also possible that the most effective way to absorb them is not through the roots. It's very likely that foliar or trunk application would be more effective since that's where jasmonates (or at least the messengers that trigger them) originate in a stem break.
 
BTW, jasmonic acid stunts growth and increases flowering, so it may be present in products we already have available (this is my hazy memory talking right now, I'm almost positive I saw it in at least one product, I just can't remember which).
 
G

good drown

i imagined it would be like applying pollen almost, but thats in my head, and alot of times wrong
 

Dank-j

Active member
They are on to something me thinks. User OldMan&theWeed had some BAD ASS threads about what's in additives and a thread on jasmonic acid that everyone should read. Makes a person think about what really is in that bottle of voodoo-magic-secret-sauce that all the nutrient companies slap a label on.
 

Jellyfish

Invertebrata Inebriata
Veteran
Take some dandelions in there and torture them, all for the benefit of your ~other~ weeds.
 
It's very possible that Canna boost contains jasmonates of some sort, same with maui lcf (I'm experimenting with this stuff next run), and as mentioned previously, harpin protein encourages production.
 
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