If you are able to you should remove one. It'll just make things easier especially if one is a male. If not no big deal I've done as many as 4 plants in one pot.
Ah well being autos changes things a bit. How big are they and how long have they been in the pot, and is this the final container they will go into?
Be very gentle, but yes I'd definitely separate them. They aren't even close to being established in that pot.